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The map label for this gene is fleQ [H]

Identifier: 78047570

GI number: 78047570

Start: 2295491

End: 2296975

Strand: Reverse

Name: fleQ [H]

Synonym: XCV2014

Alternate gene names: 78047570

Gene position: 2296975-2295491 (Counterclockwise)

Preceding gene: 78047571

Following gene: 78047569

Centisome position: 44.36

GC content: 66.67

Gene sequence:

>1485_bases
ATGAGTGAGTCCCGCATTCTGTTGATCGACAGTGACGCCGTGCGCGCCGAGCGCACCGTGTCGCTGCTCGAGTTCATGGA
CTTCAATCCCCGCTGGGTCACCGACGGCGCCGACATCAATCCTGGCCGTCACCGCCACGATGAGTGGATGGCGGTGATGG
TGGGCTCGGCGCAGGACGCCGCACAGGCCGACAAATTCTTCGACTGGCTGGCCGATGCCAAACTGCCGCCACCGGTACTG
CTGATGGAGGGCAGCCCGAGCGCGTTTGCGCAATCCCACGGCCTGCACGAAGCCAATGTGTGGGCGTTGGACACTCCCCT
GCGGCATGCGCAGCTGGAAGCGTTGCTGCGCCGTGCCAGCCTCAAGCGGCTGGACGCCGAGCACCAGGCCGGTGTGCAGC
AGGACTCCGGCCCGACCGGCAACAGCGAGGCGGTGACCCGCCTGCGCCGGCTGATCGACCAGGTGGCCGCGTTCGACACC
ACCGTGCTGGTGCTGGGCGAATCCGGCACCGGCAAGGAAGTGGTGGCGCGCGCCATCCACCAGCATTCGCCGCGGCGCGA
CGGGCCGTTTGTGGCAATCAATTGCGGCGCGATCCCGCCGGATCTGCTGGAAAGCGAACTGTTCGGCCACGAAAAAGGCG
CCTTCACGGGCGCGCTGACCACCCGCAAGGGTCGCTTCGAGATGGCCGAGGGCGGCACGCTGCTGCTGGACGAAATTGGC
GACATGAGCCTGCCGATGCAGGTCAAGCTGCTGCGCGTGCTGCAGGAGCGCAGCTTCGAGCGCGTTGGCGGCGGGCAGAC
CATCCGCTGCAACGTGCGCGTGATCGCCGCGACCCACCGCAATCTGGAATCGCGCATCAGCGATGGCCAGTTCCGCGAGG
ACCTGTTCTATCGCCTGAATGTGTTTCCGATCGAAATGCCGGCACTGCGCGAGCGCGTGGACGATCTGGCCATGCTGGTA
CAGACCATTGCGGGCCAGCTGGCACGCACCGGTCGCGGCGAAGTGCGCTTTGCCGACGAGGCCCTGCAGGCGCTGCGCAG
TTACGATTGGCCGGGCAATGTGCGCGAGCTGACCAATTTGGTCGAGCGCCTGGCGGTGCTGCATCCCGGCGGGCTGGTGC
GGGTGCAGGATCTGCCGGCGCGTTATCGCGGCGATTTCGCTTCGGCCAGTCCGGTCGAGCTGCCGCCGGAGCCGGCGTTG
GTGGCCGCACCGGTCGAAGTCAGCGCCCTGCCGAGCAATGTGGTGACGCTGCAACCAAAGGACGCCGATGCCGAACCGGC
CGCCACGTCCAGCCTGCCGGACGATGGCATCGATTTGCGCGGGCATATGGCCAATATCGAGTTGGCGTTGATCAACGAAG
CACTGGAACGCACGCAGGGCGTGGTGGCGCATGCCGCTCAGCTGCTGGGCCTGCGCCGCACCACGTTGGTGGAGAAACTG
CGCAAGTACGGCATCGATCGCGAGCAGACCGAGCTGGCCAACTGA

Upstream 100 bases:

>100_bases
GAACACGCTCTTCGCGCGGGTGCTGACAATTTTGTCAGCAAGCTGTCCTATATCCAGGAAGTCATGCCAATCCTGGAGGG
CCTGACGGAAGGAGCCAGGA

Downstream 100 bases:

>100_bases
GCCAGCACACGCTCGGGTTCCGCTCGCTGGCATAGCGCTTGCTTCAGCAGGATCACTCCGTTCGCAGCTGCCGGCTGCGG
CGGAATCCCGACATCCACCC

Product: flagellar sigma-54 dependent transcriptional activator FleQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSESRILLIDSDAVRAERTVSLLEFMDFNPRWVTDGADINPGRHRHDEWMAVMVGSAQDAAQADKFFDWLADAKLPPPVL
LMEGSPSAFAQSHGLHEANVWALDTPLRHAQLEALLRRASLKRLDAEHQAGVQQDSGPTGNSEAVTRLRRLIDQVAAFDT
TVLVLGESGTGKEVVARAIHQHSPRRDGPFVAINCGAIPPDLLESELFGHEKGAFTGALTTRKGRFEMAEGGTLLLDEIG
DMSLPMQVKLLRVLQERSFERVGGGQTIRCNVRVIAATHRNLESRISDGQFREDLFYRLNVFPIEMPALRERVDDLAMLV
QTIAGQLARTGRGEVRFADEALQALRSYDWPGNVRELTNLVERLAVLHPGGLVRVQDLPARYRGDFASASPVELPPEPAL
VAAPVEVSALPSNVVTLQPKDADAEPAATSSLPDDGIDLRGHMANIELALINEALERTQGVVAHAAQLLGLRRTTLVEKL
RKYGIDREQTELAN

Sequences:

>Translated_494_residues
MSESRILLIDSDAVRAERTVSLLEFMDFNPRWVTDGADINPGRHRHDEWMAVMVGSAQDAAQADKFFDWLADAKLPPPVL
LMEGSPSAFAQSHGLHEANVWALDTPLRHAQLEALLRRASLKRLDAEHQAGVQQDSGPTGNSEAVTRLRRLIDQVAAFDT
TVLVLGESGTGKEVVARAIHQHSPRRDGPFVAINCGAIPPDLLESELFGHEKGAFTGALTTRKGRFEMAEGGTLLLDEIG
DMSLPMQVKLLRVLQERSFERVGGGQTIRCNVRVIAATHRNLESRISDGQFREDLFYRLNVFPIEMPALRERVDDLAMLV
QTIAGQLARTGRGEVRFADEALQALRSYDWPGNVRELTNLVERLAVLHPGGLVRVQDLPARYRGDFASASPVELPPEPAL
VAAPVEVSALPSNVVTLQPKDADAEPAATSSLPDDGIDLRGHMANIELALINEALERTQGVVAHAAQLLGLRRTTLVEKL
RKYGIDREQTELAN
>Mature_493_residues
SESRILLIDSDAVRAERTVSLLEFMDFNPRWVTDGADINPGRHRHDEWMAVMVGSAQDAAQADKFFDWLADAKLPPPVLL
MEGSPSAFAQSHGLHEANVWALDTPLRHAQLEALLRRASLKRLDAEHQAGVQQDSGPTGNSEAVTRLRRLIDQVAAFDTT
VLVLGESGTGKEVVARAIHQHSPRRDGPFVAINCGAIPPDLLESELFGHEKGAFTGALTTRKGRFEMAEGGTLLLDEIGD
MSLPMQVKLLRVLQERSFERVGGGQTIRCNVRVIAATHRNLESRISDGQFREDLFYRLNVFPIEMPALRERVDDLAMLVQ
TIAGQLARTGRGEVRFADEALQALRSYDWPGNVRELTNLVERLAVLHPGGLVRVQDLPARYRGDFASASPVELPPEPALV
AAPVEVSALPSNVVTLQPKDADAEPAATSSLPDDGIDLRGHMANIELALINEALERTQGVVAHAAQLLGLRRTTLVEKLR
KYGIDREQTELAN

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=348, Percent_Identity=47.4137931034483, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788550, Length=347, Percent_Identity=42.0749279538905, Blast_Score=271, Evalue=9e-74,
Organism=Escherichia coli, GI1790299, Length=415, Percent_Identity=40.9638554216867, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1789087, Length=352, Percent_Identity=43.75, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1790437, Length=408, Percent_Identity=40.6862745098039, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI1788905, Length=351, Percent_Identity=42.4501424501425, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI1789233, Length=350, Percent_Identity=41.1428571428571, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082152, Length=229, Percent_Identity=51.528384279476, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1786524, Length=242, Percent_Identity=48.7603305785124, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI87081872, Length=351, Percent_Identity=35.6125356125356, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1787583, Length=399, Percent_Identity=32.8320802005013, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789828, Length=372, Percent_Identity=30.9139784946237, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI87081858, Length=344, Percent_Identity=30.5232558139535, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54230; Mature: 54099

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESRILLIDSDAVRAERTVSLLEFMDFNPRWVTDGADINPGRHRHDEWMAVMVGSAQDA
CCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEECCCHHH
AQADKFFDWLADAKLPPPVLLMEGSPSAFAQSHGLHEANVWALDTPLRHAQLEALLRRAS
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHH
LKRLDAEHQAGVQQDSGPTGNSEAVTRLRRLIDQVAAFDTTVLVLGESGTGKEVVARAIH
HHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
QHSPRRDGPFVAINCGAIPPDLLESELFGHEKGAFTGALTTRKGRFEMAEGGTLLLDEIG
HCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCEEEEECCC
DMSLPMQVKLLRVLQERSFERVGGGQTIRCNVRVIAATHRNLESRISDGQFREDLFYRLN
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHCHHHHCCCCHHHHHHHEEEE
VFPIEMPALRERVDDLAMLVQTIAGQLARTGRGEVRFADEALQALRSYDWPGNVRELTNL
EEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHH
VERLAVLHPGGLVRVQDLPARYRGDFASASPVELPPEPALVAAPVEVSALPSNVVTLQPK
HHHHHHHCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCEEECCCEEECCCCCEEEECCC
DADAEPAATSSLPDDGIDLRGHMANIELALINEALERTQGVVAHAAQLLGLRRTTLVEKL
CCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKYGIDREQTELAN
HHHCCCHHHHHCCC
>Mature Secondary Structure 
SESRILLIDSDAVRAERTVSLLEFMDFNPRWVTDGADINPGRHRHDEWMAVMVGSAQDA
CCCEEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEECCCHHH
AQADKFFDWLADAKLPPPVLLMEGSPSAFAQSHGLHEANVWALDTPLRHAQLEALLRRAS
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHH
LKRLDAEHQAGVQQDSGPTGNSEAVTRLRRLIDQVAAFDTTVLVLGESGTGKEVVARAIH
HHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
QHSPRRDGPFVAINCGAIPPDLLESELFGHEKGAFTGALTTRKGRFEMAEGGTLLLDEIG
HCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCEEEEECCC
DMSLPMQVKLLRVLQERSFERVGGGQTIRCNVRVIAATHRNLESRISDGQFREDLFYRLN
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHCHHHHCCCCHHHHHHHEEEE
VFPIEMPALRERVDDLAMLVQTIAGQLARTGRGEVRFADEALQALRSYDWPGNVRELTNL
EEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHH
VERLAVLHPGGLVRVQDLPARYRGDFASASPVELPPEPALVAAPVEVSALPSNVVTLQPK
HHHHHHHCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCEEECCCEEECCCCCEEEECCC
DADAEPAATSSLPDDGIDLRGHMANIELALINEALERTQGVVAHAAQLLGLRRTTLVEKL
CCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKYGIDREQTELAN
HHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2881918; 11481430; 8478331 [H]