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The map label for this gene is dosP [H]

Identifier: 78047485

GI number: 78047485

Start: 2182300

End: 2184600

Strand: Reverse

Name: dosP [H]

Synonym: XCV1929

Alternate gene names: 78047485

Gene position: 2184600-2182300 (Counterclockwise)

Preceding gene: 78047486

Following gene: 78047480

Centisome position: 42.19

GC content: 65.58

Gene sequence:

>2301_bases
GTGGGCGCTGGCGTGGATGCAGCGATGGACCGCACGCCGCTGACGGCTGAGCCGATCCATGCCAACGCCCCAGGCACGGG
CCAGATCAGGCCCGGCCCGGTCACCGCCGGCATGGCCAAGGCCGGCGAACAGCCCGACCAACGCCGCGCGCGTCTGCTCG
CCGAAGCCCTGGATGGCAGCAGCAGCGCCATTCTCATCTGCGACCTGCAATCGCGCCTGCTGTACGCCAACGACGGCTTC
CAGCGCATGTTCGGCTACACCGAAGCCGAGCTGGTCGGTCTGCGTCCCTCCGATGTCCTGACCCGCGCGCAAAGCGACCA
AAGCGAGGTCGCTCGCGTTCGCGATCGCGCCGATGCCTTGCAGCACACCCGTGCCGACCTGCTGGTCTATCGCAAGGACG
GCACGCCGCTGTGGATCTCGCTGAACTTCAACCCGATCTACGACGCCAACCACGCTCCCTCGCATTACGTGGCGGTACTG
ACCGACATCACCGACACCAAGATGCACGAGGTGTTGCAGCACCGCGTGCTGGAGGCCCTGGTGCAGGAACGCCCGTTGAT
CGAGGTGATGACGCTGATTTGCACCGAGGTCGAACGCATCGCACCGGATGTGCTGGCCACGGTGATGAGTGCGGACAATC
AAGGTCGCCTGCATTCGCTGGCTGCGCCCAGCCTGCCGCCCGAATACGCCGACGCAGTCAACGGCTTGAAGATCGGCCCG
GGCACCGGCGCCTGCGGCACCGCGGCCTGGCGCGGTCGCGCGGTGATGGTGGAAGACATCGCCACCGACCCATTGTTCGA
GGCGTATCGCGACACGCTCTTGCCTTGGGGCCTGCGCGCCTGCTGGAGCACGCCGATCCGCTCCAACAGCGCGCGCGTGC
TCGGCACCTTCGCGCTGTATTACCGCAAGCCGCAACGTGCCGATGCCTGGCATATGCGGCTCACCGATTTGTGCCAGCAG
CTGTGCACATTGGCGCTGGAACGCGAGCAGATCCGCCAGCGCGTGCATCAACTGGCGTTCTACGACACGCTCACGGGCCT
TCCCAACCGCATCATGTTCAGCGCGCGTGCCGAGCAGGCACTGACCCAGGCCGAATACGCCGGCGAACCGGCCGCGTTGA
TGTTCATCAACATCGATCGCTTCAAGCTCATCAACGATAGCCAGGGGCACGCCGCAGGCGATGGCCTGCTGCGCGATATC
GCCTTGCGCGTCAACGACCAGATCACCGCCACGGCGATGCTGGCGCGGCTATCCGGCGACGAGTTCGCGCTCGCGCTGCC
GCAATGCAGCGCAGAGCAGGCCAGTGCCGCCGCCGAGCGTTTGTTGGTCAGCATCGCCAGCCCGCTGCCGGTTGGCCATA
TGACCGTGCATCCCTCGGCCAGTATCGGCGTGGCGATGTTTCCCGAGGATGGCCACGACATCAACATCCTGCTGCGCCAT
GCCGACTTGGCGATGAATCGCGCCAAGAAGGAAGGCGGTGGGCGCTTCCGCTTTTTCAGTTCCGACATGAACCGCATGGC
GCAGGAACGCGTGGCCTTGGAGGCCGAGCTGCGCCAGGCCATTGGCCGCGATCAATTGCAGCTGCACTACCAGCCGCAGA
TGCACAGCGCCGCGCCGCATGCCCTGTATGGCGTGGAAGCGCTGCTGCGCTGGAATCATCCGCAACTGGGCGCGGTATCG
CCGGCGCGGTTTCTGCCGGTGGCTGAAGAGCAAGGCATGATGCCGCAGCTCAACGCCTGGGTACTGCGACGTGCCTGCGA
ACAGGTCGCGGACTGGCGCCAGCGTGGCGTGCCGGTCGCGCGCGTGTCGGTGAATCTGTCGGCAAGCGGCTTCGAAAGCC
CGCGCATGCTTCCCGACCTGCTGAAGCTGATCGCCGAACTGGGCGTGCAGCCGTCCGATCTCACCCTGGAACTGACCGAA
AGCGTGATGCTGTCCGGGCAACCGGGCGTGCTGGAAAACCTGCACGCCATTCGCGAAGCCGGCATCTCGCTGTCGCTGGA
CGATTTCGGCACTGGCTATTCCAGCCTGAGCCACTTGCATCGTCTGCCGATCGACGAACTCAAACTGGACATGAGCTTCG
TCCGCGATATCGAACACAGCGAAGACGCACGCGCGCTCACCACCTCGGTGCTGCGCATCGGCGAGCACCTGCGCAAGCAC
GTGGTCGCCGAAGGTGTGGAAAACGAAGCGCAGCGCCTGTTCCTCGCCGACCTGGGTTGCGAGGTGCTGCAAGGCTATCT
GTTCTCGCGGCCGCTACCGGCCCAGGCACTGGAAGCCTGGCTGGGCGCGCAACCGTCATAA

Upstream 100 bases:

>100_bases
GCGCTTGCTCGCATTGTCGGCGCAGGCGCGCTGATCCGGCATGGCCGCGCATCCCTCGCACCCGACCAACCCAACGCTTC
CTCCGGCACCGGGAAGCGTG

Downstream 100 bases:

>100_bases
CGCAACCGCCGAACGATCACCGCTTCGAACTTCCTTCCAGCCAGGAAAGGCGGGCCATGGCGTGGCAAGTCATGAGCCTC
CTCCTGCAGAAGAGCAGCGC

Product: putative c-di-GMP phosphodiesterase or signal transduction protein

Products: NA

Alternate protein names: Direct oxygen sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, [H]

Number of amino acids: Translated: 766; Mature: 765

Protein sequence:

>766_residues
MGAGVDAAMDRTPLTAEPIHANAPGTGQIRPGPVTAGMAKAGEQPDQRRARLLAEALDGSSSAILICDLQSRLLYANDGF
QRMFGYTEAELVGLRPSDVLTRAQSDQSEVARVRDRADALQHTRADLLVYRKDGTPLWISLNFNPIYDANHAPSHYVAVL
TDITDTKMHEVLQHRVLEALVQERPLIEVMTLICTEVERIAPDVLATVMSADNQGRLHSLAAPSLPPEYADAVNGLKIGP
GTGACGTAAWRGRAVMVEDIATDPLFEAYRDTLLPWGLRACWSTPIRSNSARVLGTFALYYRKPQRADAWHMRLTDLCQQ
LCTLALEREQIRQRVHQLAFYDTLTGLPNRIMFSARAEQALTQAEYAGEPAALMFINIDRFKLINDSQGHAAGDGLLRDI
ALRVNDQITATAMLARLSGDEFALALPQCSAEQASAAAERLLVSIASPLPVGHMTVHPSASIGVAMFPEDGHDINILLRH
ADLAMNRAKKEGGGRFRFFSSDMNRMAQERVALEAELRQAIGRDQLQLHYQPQMHSAAPHALYGVEALLRWNHPQLGAVS
PARFLPVAEEQGMMPQLNAWVLRRACEQVADWRQRGVPVARVSVNLSASGFESPRMLPDLLKLIAELGVQPSDLTLELTE
SVMLSGQPGVLENLHAIREAGISLSLDDFGTGYSSLSHLHRLPIDELKLDMSFVRDIEHSEDARALTTSVLRIGEHLRKH
VVAEGVENEAQRLFLADLGCEVLQGYLFSRPLPAQALEAWLGAQPS

Sequences:

>Translated_766_residues
MGAGVDAAMDRTPLTAEPIHANAPGTGQIRPGPVTAGMAKAGEQPDQRRARLLAEALDGSSSAILICDLQSRLLYANDGF
QRMFGYTEAELVGLRPSDVLTRAQSDQSEVARVRDRADALQHTRADLLVYRKDGTPLWISLNFNPIYDANHAPSHYVAVL
TDITDTKMHEVLQHRVLEALVQERPLIEVMTLICTEVERIAPDVLATVMSADNQGRLHSLAAPSLPPEYADAVNGLKIGP
GTGACGTAAWRGRAVMVEDIATDPLFEAYRDTLLPWGLRACWSTPIRSNSARVLGTFALYYRKPQRADAWHMRLTDLCQQ
LCTLALEREQIRQRVHQLAFYDTLTGLPNRIMFSARAEQALTQAEYAGEPAALMFINIDRFKLINDSQGHAAGDGLLRDI
ALRVNDQITATAMLARLSGDEFALALPQCSAEQASAAAERLLVSIASPLPVGHMTVHPSASIGVAMFPEDGHDINILLRH
ADLAMNRAKKEGGGRFRFFSSDMNRMAQERVALEAELRQAIGRDQLQLHYQPQMHSAAPHALYGVEALLRWNHPQLGAVS
PARFLPVAEEQGMMPQLNAWVLRRACEQVADWRQRGVPVARVSVNLSASGFESPRMLPDLLKLIAELGVQPSDLTLELTE
SVMLSGQPGVLENLHAIREAGISLSLDDFGTGYSSLSHLHRLPIDELKLDMSFVRDIEHSEDARALTTSVLRIGEHLRKH
VVAEGVENEAQRLFLADLGCEVLQGYLFSRPLPAQALEAWLGAQPS
>Mature_765_residues
GAGVDAAMDRTPLTAEPIHANAPGTGQIRPGPVTAGMAKAGEQPDQRRARLLAEALDGSSSAILICDLQSRLLYANDGFQ
RMFGYTEAELVGLRPSDVLTRAQSDQSEVARVRDRADALQHTRADLLVYRKDGTPLWISLNFNPIYDANHAPSHYVAVLT
DITDTKMHEVLQHRVLEALVQERPLIEVMTLICTEVERIAPDVLATVMSADNQGRLHSLAAPSLPPEYADAVNGLKIGPG
TGACGTAAWRGRAVMVEDIATDPLFEAYRDTLLPWGLRACWSTPIRSNSARVLGTFALYYRKPQRADAWHMRLTDLCQQL
CTLALEREQIRQRVHQLAFYDTLTGLPNRIMFSARAEQALTQAEYAGEPAALMFINIDRFKLINDSQGHAAGDGLLRDIA
LRVNDQITATAMLARLSGDEFALALPQCSAEQASAAAERLLVSIASPLPVGHMTVHPSASIGVAMFPEDGHDINILLRHA
DLAMNRAKKEGGGRFRFFSSDMNRMAQERVALEAELRQAIGRDQLQLHYQPQMHSAAPHALYGVEALLRWNHPQLGAVSP
ARFLPVAEEQGMMPQLNAWVLRRACEQVADWRQRGVPVARVSVNLSASGFESPRMLPDLLKLIAELGVQPSDLTLELTES
VMLSGQPGVLENLHAIREAGISLSLDDFGTGYSSLSHLHRLPIDELKLDMSFVRDIEHSEDARALTTSVLRIGEHLRKHV
VAEGVENEAQRLFLADLGCEVLQGYLFSRPLPAQALEAWLGAQPS

Specific function: In association with DosC is involved in the production and removal of the second messenger c-di-GMP in response to changing O(2) levels. Has phosphodiesterase (PDE) activity with c- di-GMP (PubMed:15995192), very poor activity on cAMP (PubMed:15995192) bu

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=721, Percent_Identity=28.9875173370319, Blast_Score=337, Evalue=2e-93,
Organism=Escherichia coli, GI1787541, Length=434, Percent_Identity=36.8663594470046, Blast_Score=298, Evalue=9e-82,
Organism=Escherichia coli, GI226510982, Length=451, Percent_Identity=30.1552106430155, Blast_Score=186, Evalue=5e-48,
Organism=Escherichia coli, GI1790496, Length=265, Percent_Identity=31.6981132075472, Blast_Score=147, Evalue=4e-36,
Organism=Escherichia coli, GI87081845, Length=264, Percent_Identity=31.4393939393939, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI87081743, Length=245, Percent_Identity=35.1020408163265, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI1788502, Length=249, Percent_Identity=32.9317269076305, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081980, Length=263, Percent_Identity=33.0798479087452, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1788849, Length=459, Percent_Identity=25.7080610021786, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1787055, Length=251, Percent_Identity=29.4820717131474, Blast_Score=123, Evalue=5e-29,
Organism=Escherichia coli, GI1786507, Length=222, Percent_Identity=29.7297297297297, Blast_Score=122, Evalue=9e-29,
Organism=Escherichia coli, GI1788381, Length=203, Percent_Identity=33.9901477832512, Blast_Score=106, Evalue=7e-24,
Organism=Escherichia coli, GI87082096, Length=247, Percent_Identity=27.9352226720648, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI145693134, Length=195, Percent_Identity=29.7435897435897, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87081977, Length=225, Percent_Identity=28, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=34.1040462427746, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=33.5329341317365, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=197, Percent_Identity=29.4416243654822, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789650, Length=478, Percent_Identity=21.3389121338912, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=32.0987654320988, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1787262, Length=269, Percent_Identity=25.6505576208178, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=29.8136645962733, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788956, Length=134, Percent_Identity=36.5671641791045, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=28.0487804878049, Blast_Score=68, Evalue=3e-12,
Organism=Escherichia coli, GI1787410, Length=150, Percent_Identity=27.3333333333333, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR012226
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00989 PAS; PF08448 PAS_4 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 84126; Mature: 83995

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAGVDAAMDRTPLTAEPIHANAPGTGQIRPGPVTAGMAKAGEQPDQRRARLLAEALDGS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCC
SSAILICDLQSRLLYANDGFQRMFGYTEAELVGLRPSDVLTRAQSDQSEVARVRDRADAL
CCEEEEEECCCCEEECCCCHHHHHCCCCCEEECCCCHHHHHHCCCCHHHHHHHHHHHHHH
QHTRADLLVYRKDGTPLWISLNFNPIYDANHAPSHYVAVLTDITDTKMHEVLQHRVLEAL
HHCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
VQERPLIEVMTLICTEVERIAPDVLATVMSADNQGRLHSLAAPSLPPEYADAVNGLKIGP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEECC
GTGACGTAAWRGRAVMVEDIATDPLFEAYRDTLLPWGLRACWSTPIRSNSARVLGTFALY
CCCCCCCHHHCCCEEEEEHHCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHH
YRKPQRADAWHMRLTDLCQQLCTLALEREQIRQRVHQLAFYDTLTGLPNRIMFSARAEQA
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHH
LTQAEYAGEPAALMFINIDRFKLINDSQGHAAGDGLLRDIALRVNDQITATAMLARLSGD
HHHHHHCCCCEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC
EFALALPQCSAEQASAAAERLLVSIASPLPVGHMTVHPSASIGVAMFPEDGHDINILLRH
CEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEECCCCCCCEEEEEEH
ADLAMNRAKKEGGGRFRFFSSDMNRMAQERVALEAELRQAIGRDQLQLHYQPQMHSAAPH
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCH
ALYGVEALLRWNHPQLGAVSPARFLPVAEEQGMMPQLNAWVLRRACEQVADWRQRGVPVA
HHHHHHHHHHCCCCCCCCCCHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
RVSVNLSASGFESPRMLPDLLKLIAELGVQPSDLTLELTESVMLSGQPGVLENLHAIREA
EEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHCCCCCCHHHHHHHHHHC
GISLSLDDFGTGYSSLSHLHRLPIDELKLDMSFVRDIEHSEDARALTTSVLRIGEHLRKH
CCEEEEHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VVAEGVENEAQRLFLADLGCEVLQGYLFSRPLPAQALEAWLGAQPS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
GAGVDAAMDRTPLTAEPIHANAPGTGQIRPGPVTAGMAKAGEQPDQRRARLLAEALDGS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCC
SSAILICDLQSRLLYANDGFQRMFGYTEAELVGLRPSDVLTRAQSDQSEVARVRDRADAL
CCEEEEEECCCCEEECCCCHHHHHCCCCCEEECCCCHHHHHHCCCCHHHHHHHHHHHHHH
QHTRADLLVYRKDGTPLWISLNFNPIYDANHAPSHYVAVLTDITDTKMHEVLQHRVLEAL
HHCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
VQERPLIEVMTLICTEVERIAPDVLATVMSADNQGRLHSLAAPSLPPEYADAVNGLKIGP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEECC
GTGACGTAAWRGRAVMVEDIATDPLFEAYRDTLLPWGLRACWSTPIRSNSARVLGTFALY
CCCCCCCHHHCCCEEEEEHHCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHH
YRKPQRADAWHMRLTDLCQQLCTLALEREQIRQRVHQLAFYDTLTGLPNRIMFSARAEQA
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHH
LTQAEYAGEPAALMFINIDRFKLINDSQGHAAGDGLLRDIALRVNDQITATAMLARLSGD
HHHHHHCCCCEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC
EFALALPQCSAEQASAAAERLLVSIASPLPVGHMTVHPSASIGVAMFPEDGHDINILLRH
CEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEECCCCCCCEEEEEEH
ADLAMNRAKKEGGGRFRFFSSDMNRMAQERVALEAELRQAIGRDQLQLHYQPQMHSAAPH
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCH
ALYGVEALLRWNHPQLGAVSPARFLPVAEEQGMMPQLNAWVLRRACEQVADWRQRGVPVA
HHHHHHHHHHCCCCCCCCCCHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
RVSVNLSASGFESPRMLPDLLKLIAELGVQPSDLTLELTESVMLSGQPGVLENLHAIREA
EEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHCCCCCCHHHHHHHHHHC
GISLSLDDFGTGYSSLSHLHRLPIDELKLDMSFVRDIEHSEDARALTTSVLRIGEHLRKH
CCEEEEHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VVAEGVENEAQRLFLADLGCEVLQGYLFSRPLPAQALEAWLGAQPS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]