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The map label for this gene is rpfF [H]

Identifier: 78047476

GI number: 78047476

Start: 2170961

End: 2171830

Strand: Reverse

Name: rpfF [H]

Synonym: XCV1920

Alternate gene names: 78047476

Gene position: 2171830-2170961 (Counterclockwise)

Preceding gene: 78047477

Following gene: 78047469

Centisome position: 41.94

GC content: 61.72

Gene sequence:

>870_bases
ATGTCTGCAGTTCAACCCTTCATTCGCACCAACATCGGCTCGACCCTACGCATCATCGAAGAACCGCAGCGTGACGTGTA
CTGGATCCATATGCATGCCGACCTGGCCACAAACCCGGGGCGTGCCTGCTTTTCGACGCGCCTGGTCGACGACATTACCG
GCTATCAGACCAACCTTGGTCAACGCCTGAACAACGCCGGCGTGCTGGCTCCGCACGTGGTGCTGGCATCGGACAGCGAT
GTGTTCAACCTGGGCGGCGATCTGGCGCTGTTCTGTCAACTCATTCGCGAAGGCGACCGTGCTCGACTGCTTAATTACGC
CCAACGCTGTGTACGCGGTGTGCACGCGTTCCATGTCGGCCTGGGCGCACGCGCGCACAGCATCGCCCTGGTGCAGGGCA
ATGCCCTCGGCGGCGGATTCGAAGCGGCGTTGAGCTGCCACACCATCGTCGCCGAGGAAGGGGTGATGATGGGATTGCCC
GAAGTGCTGTTCGACCTGTTCCCGGGGATGGGCGCCTACTCTTTCATGTGCCAGCGTGTCAGTGCGCAACTGGCGCAAAA
AATCATGCTCGAAGGCAATCTGTATTCTGCCGAGGAGTTGCTCGGCATGGGCCTAGTCGATCGCGTCGTGCCGCGCGGTC
AGGGCGTGGCGGCGGTCGAACAAGTGATCCGCGAAAGCAAGCGCACGCCGCATGCCTGGGCGGCAATGCAGCAAGTGCGC
GAAATGACCACGGCGGTGCCGCTGCAGGAAATGATGCGCATCACCGAAATCTGGGTCGATACGGCAATGCAGTTGGGCGA
AAAGTCATTGCGCACCATGGACCGTCTGGTTCGCGCGCAATCGCGCCGCTCAGGGCTTGACGCCGGCTGA

Upstream 100 bases:

>100_bases
CTTTTGCACGCATAGAATCCGATGTCGGCGCACGCTCACGCGTTCAACCTCATCTCCGTCCGAAAGGCGGCCGGCCGGAC
GGTCATCACGGAGAACCACC

Downstream 100 bases:

>100_bases
TGCGTCGGCGGGCTATTCACTACTCCGGGGGGAGCGCTCGTCGTCGTTGGCGCTTTGTACGCGCGCGGCGAGTGCATCCT
TTCCGGTCTGCAATTGGTCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSAVQPFIRTNIGSTLRIIEEPQRDVYWIHMHADLATNPGRACFSTRLVDDITGYQTNLGQRLNNAGVLAPHVVLASDSD
VFNLGGDLALFCQLIREGDRARLLNYAQRCVRGVHAFHVGLGARAHSIALVQGNALGGGFEAALSCHTIVAEEGVMMGLP
EVLFDLFPGMGAYSFMCQRVSAQLAQKIMLEGNLYSAEELLGMGLVDRVVPRGQGVAAVEQVIRESKRTPHAWAAMQQVR
EMTTAVPLQEMMRITEIWVDTAMQLGEKSLRTMDRLVRAQSRRSGLDAG

Sequences:

>Translated_289_residues
MSAVQPFIRTNIGSTLRIIEEPQRDVYWIHMHADLATNPGRACFSTRLVDDITGYQTNLGQRLNNAGVLAPHVVLASDSD
VFNLGGDLALFCQLIREGDRARLLNYAQRCVRGVHAFHVGLGARAHSIALVQGNALGGGFEAALSCHTIVAEEGVMMGLP
EVLFDLFPGMGAYSFMCQRVSAQLAQKIMLEGNLYSAEELLGMGLVDRVVPRGQGVAAVEQVIRESKRTPHAWAAMQQVR
EMTTAVPLQEMMRITEIWVDTAMQLGEKSLRTMDRLVRAQSRRSGLDAG
>Mature_288_residues
SAVQPFIRTNIGSTLRIIEEPQRDVYWIHMHADLATNPGRACFSTRLVDDITGYQTNLGQRLNNAGVLAPHVVLASDSDV
FNLGGDLALFCQLIREGDRARLLNYAQRCVRGVHAFHVGLGARAHSIALVQGNALGGGFEAALSCHTIVAEEGVMMGLPE
VLFDLFPGMGAYSFMCQRVSAQLAQKIMLEGNLYSAEELLGMGLVDRVVPRGQGVAAVEQVIRESKRTPHAWAAMQQVRE
MTTAVPLQEMMRITEIWVDTAMQLGEKSLRTMDRLVRAQSRRSGLDAG

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1788682, Length=180, Percent_Identity=28.8888888888889, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787659, Length=179, Percent_Identity=31.8435754189944, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790281, Length=170, Percent_Identity=28.8235294117647, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17558304, Length=186, Percent_Identity=26.8817204301075, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006180
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR012802
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =1.1.1.35 [H]

Molecular weight: Translated: 31676; Mature: 31545

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVQPFIRTNIGSTLRIIEEPQRDVYWIHMHADLATNPGRACFSTRLVDDITGYQTNLG
CCCCHHHHHHCCCCHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
QRLNNAGVLAPHVVLASDSDVFNLGGDLALFCQLIREGDRARLLNYAQRCVRGVHAFHVG
HHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LGARAHSIALVQGNALGGGFEAALSCHTIVAEEGVMMGLPEVLFDLFPGMGAYSFMCQRV
CCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHH
SAQLAQKIMLEGNLYSAEELLGMGLVDRVVPRGQGVAAVEQVIRESKRTPHAWAAMQQVR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
EMTTAVPLQEMMRITEIWVDTAMQLGEKSLRTMDRLVRAQSRRSGLDAG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SAVQPFIRTNIGSTLRIIEEPQRDVYWIHMHADLATNPGRACFSTRLVDDITGYQTNLG
CCCHHHHHHCCCCHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
QRLNNAGVLAPHVVLASDSDVFNLGGDLALFCQLIREGDRARLLNYAQRCVRGVHAFHVG
HHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LGARAHSIALVQGNALGGGFEAALSCHTIVAEEGVMMGLPEVLFDLFPGMGAYSFMCQRV
CCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHH
SAQLAQKIMLEGNLYSAEELLGMGLVDRVVPRGQGVAAVEQVIRESKRTPHAWAAMQQVR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
EMTTAVPLQEMMRITEIWVDTAMQLGEKSLRTMDRLVRAQSRRSGLDAG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA