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The map label for this gene is thiD [H]

Identifier: 78047340

GI number: 78047340

Start: 2007224

End: 2008111

Strand: Reverse

Name: thiD [H]

Synonym: XCV1784

Alternate gene names: 78047340

Gene position: 2008111-2007224 (Counterclockwise)

Preceding gene: 78047341

Following gene: 78047339

Centisome position: 38.78

GC content: 67.79

Gene sequence:

>888_bases
ATGTCGCCTACCTGCAGTGGCGCCGCTGGCGCACAGGATGATGCGCACATCCTCCGGCCAGCAGTGCAGAATCGCCACAT
GACTACGCCCAGTACGCTGTCCGCATTGAGCATCGCCGGCTCCGACTCCGGGGGCGGCGCCGGCATCCAGGCCGACCTCA
AGACCTTCGCCGCGCACCGCGTGCACGGCTTGTCGGCGATTACCGCATTGACCGCGCAACACACCCGCGCCGTCACCGCC
GTGCATCTTCCACCGTTGTCGTTTTTGCAGGCGCAGGTGGACGCCTGCTTTGCCGATTTCGAGATCCAGGCGGTCAAGCT
CGGCATGCTGGCCAATGCCGACGTCATTCATTGCGTGGCCGACCTGCTTGAGCGATATCGCCCACCGTTCGTGGTGCTCG
ACCCGGTGATGGTGTCCACCAGCGGCGCGCGCCTGCTCGAAGATGCGGCACTGGATGCGCTGCGCACGCGCTTGCTGCCG
CTGGCCACCCTGGTCACCCCCAACACGCCCGAGGCCGAGTTGCTGACCGGCCGCCGCATCGACAGCGCCGACGCCGCCGA
ACATGCCACCGCCGCCTTGCTCGACCTAGGCGCCAACGCCGTGCTGCTCAAGGGCGGACATCTGCACGAAGGCGCACGCG
TGCTCGACCGCTTCGATGACGGCGTGACCCAGGATGTGTTCATGCACCCGCGCCTGCAGGTCGATGCGCACGGCACCGGC
TGCAGCCTGTCGGCAGCCATCGCCGCGCAGCTGTGCCAGGGCCTGTCGCTGCTCAATGCCTGCGAGGCAGCGATCGATTA
CGTTGCACGCGCCATCCGCATTGGCCAACGCCCCGGCCACAGCGATGTGCTGGTGCTCGACCACTTCGGCGCCGCGCCCG
GCGCATGA

Upstream 100 bases:

>100_bases
TTTCTCGGCGGCAGCATCCTCGGAACCTTGCTGTTCCAGCGCTGGCAGTACGACGCGCTCTACCTGCCCGCCAGCCTCAC
CGGATTGACCGGCGCCGGTT

Downstream 100 bases:

>100_bases
GCGTGCAGACCAACACCCTGCTCGCCAGCGCTGCAGATGGGCATCGCTGGCAGGTCATCCGTTGCGCACCAACGCAGCCA
GCTGCAACGCTGCTGTGGCT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSPTCSGAAGAQDDAHILRPAVQNRHMTTPSTLSALSIAGSDSGGGAGIQADLKTFAAHRVHGLSAITALTAQHTRAVTA
VHLPPLSFLQAQVDACFADFEIQAVKLGMLANADVIHCVADLLERYRPPFVVLDPVMVSTSGARLLEDAALDALRTRLLP
LATLVTPNTPEAELLTGRRIDSADAAEHATAALLDLGANAVLLKGGHLHEGARVLDRFDDGVTQDVFMHPRLQVDAHGTG
CSLSAAIAAQLCQGLSLLNACEAAIDYVARAIRIGQRPGHSDVLVLDHFGAAPGA

Sequences:

>Translated_295_residues
MSPTCSGAAGAQDDAHILRPAVQNRHMTTPSTLSALSIAGSDSGGGAGIQADLKTFAAHRVHGLSAITALTAQHTRAVTA
VHLPPLSFLQAQVDACFADFEIQAVKLGMLANADVIHCVADLLERYRPPFVVLDPVMVSTSGARLLEDAALDALRTRLLP
LATLVTPNTPEAELLTGRRIDSADAAEHATAALLDLGANAVLLKGGHLHEGARVLDRFDDGVTQDVFMHPRLQVDAHGTG
CSLSAAIAAQLCQGLSLLNACEAAIDYVARAIRIGQRPGHSDVLVLDHFGAAPGA
>Mature_294_residues
SPTCSGAAGAQDDAHILRPAVQNRHMTTPSTLSALSIAGSDSGGGAGIQADLKTFAAHRVHGLSAITALTAQHTRAVTAV
HLPPLSFLQAQVDACFADFEIQAVKLGMLANADVIHCVADLLERYRPPFVVLDPVMVSTSGARLLEDAALDALRTRLLPL
ATLVTPNTPEAELLTGRRIDSADAAEHATAALLDLGANAVLLKGGHLHEGARVLDRFDDGVTQDVFMHPRLQVDAHGTGC
SLSAAIAAQLCQGLSLLNACEAAIDYVARAIRIGQRPGHSDVLVLDHFGAAPGA

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=242, Percent_Identity=42.5619834710744, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1788758, Length=193, Percent_Identity=29.0155440414508, Blast_Score=61, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6324997, Length=254, Percent_Identity=36.2204724409449, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6325378, Length=254, Percent_Identity=31.8897637795276, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324517, Length=254, Percent_Identity=34.251968503937, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 30679; Mature: 30548

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTCSGAAGAQDDAHILRPAVQNRHMTTPSTLSALSIAGSDSGGGAGIQADLKTFAAHR
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHEEECCCCCCCCCCHHHHHHHHHHHH
VHGLSAITALTAQHTRAVTAVHLPPLSFLQAQVDACFADFEIQAVKLGMLANADVIHCVA
HHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHCCEEEEEEECHHCCCHHHHHHH
DLLERYRPPFVVLDPVMVSTSGARLLEDAALDALRTRLLPLATLVTPNTPEAELLTGRRI
HHHHHCCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHCCCCC
DSADAAEHATAALLDLGANAVLLKGGHLHEGARVLDRFDDGVTQDVFMHPRLQVDAHGTG
CCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHEECCCEEEECCCCC
CSLSAAIAAQLCQGLSLLNACEAAIDYVARAIRIGQRPGHSDVLVLDHFGAAPGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
SPTCSGAAGAQDDAHILRPAVQNRHMTTPSTLSALSIAGSDSGGGAGIQADLKTFAAHR
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHEEECCCCCCCCCCHHHHHHHHHHHH
VHGLSAITALTAQHTRAVTAVHLPPLSFLQAQVDACFADFEIQAVKLGMLANADVIHCVA
HHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHCCEEEEEEECHHCCCHHHHHHH
DLLERYRPPFVVLDPVMVSTSGARLLEDAALDALRTRLLPLATLVTPNTPEAELLTGRRI
HHHHHCCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHCCCCC
DSADAAEHATAALLDLGANAVLLKGGHLHEGARVLDRFDDGVTQDVFMHPRLQVDAHGTG
CCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHEECCCEEEECCCCC
CSLSAAIAAQLCQGLSLLNACEAAIDYVARAIRIGQRPGHSDVLVLDHFGAAPGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA