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The map label for this gene is smaIM [H]

Identifier: 78046666

GI number: 78046666

Start: 1243600

End: 1244499

Strand: Reverse

Name: smaIM [H]

Synonym: XCV1110

Alternate gene names: 78046666

Gene position: 1244499-1243600 (Counterclockwise)

Preceding gene: 78046667

Following gene: 78046658

Centisome position: 24.03

GC content: 51.67

Gene sequence:

>900_bases
ATGCTTCTGATTGTTAACGAAAACGAACCGGACACATCCACACATACGGATGAGCCAATCCAAGAGCCTGCGAGAGGACG
CCTTGTCGTCGGCGATGCCCGTAAGGTCTTGGCAGGCATGGCTGACAAGAGCTTCCAATGCTGCATCACCTCTCCCCCGT
ATTGGGGACTGCGGGACTATGGAGTAGACGGTCAGATCGGCGCAGAACCGACGTTGGAAGCATACATAGAAGACTTGGTT
GCTCTATTTCGAGAAGTGCGTCGCACCTTACGTGACGATGGAACGCTGTGGCTTAACATTGGGGACGGCTTCACAAGTGG
CGGTCGCACTTGGCGAGCACCAGACAAAAAGAACAAAGGTCGCGCAATGGAGTATCGAGCGCCGACTCCTGATGGACTCA
AGCCAAAAGATTTGATCGGCGTTCCCTGGCGGCTGGCCATCGCGCTTCAGGCAGATGGCTGGTATTTACGCGCCGATATC
ATTTGGCAGAAGCCTAACTGCCAGCCGGAATCTGTGAAAGATAGGCCAACCCGATCGCACGAGTATGTTTTTCTACTTAC
CAAATCGGAACGCTATTATTACAACAGCGATGCTGTGAAAGAGCTTACAGCCGATGGAAAGCATACAAAAAATCGTCGAT
CGGTCTGGGCTATCAATACAGAACCCTACGGGGGCAACCACTTTGCGGTGTTTCCCACGGAGCTTGTGCGTCTATGCATG
GAAGCTGGATCCAAAAAAGAGGAGGTAGTGCTTGACCCTTTCTTTGGATCCGGAACCGTCGGTCAAGTCGCTCGTGATAA
TGGCCGGAAATTTGTAGGCATTGAACTATCACCAGATTACGCAGAGCTAGCAAGAAAGAGACTGGCTTCGAAAAGCAACT
CCCGAAAGTTACAACGTTGA

Upstream 100 bases:

>100_bases
CATCTGGCCCATCCAGCCTTGGTCGTTCCTCTTGGCACCCTGCTTGACGTAAAGGCAAATGCACGGGAACCTTTGCACTG
CGGTCTTCCGACGAACATCT

Downstream 100 bases:

>100_bases
ACTTTCGAATTGCCAAGTGGCCACAACCGAACACAGGTTCTTCTTTAGATGCTTAATCTGAAGTTTCCGCTCCCCGCCAC
CATCGAAGTAGAGCGCGAAC

Product: site-specific DNA-methyltransferase (cytosine-N(4)-specific)

Products: NA

Alternate protein names: M.SmaI; N-4 cytosine-specific methyltransferase SmaI [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLLIVNENEPDTSTHTDEPIQEPARGRLVVGDARKVLAGMADKSFQCCITSPPYWGLRDYGVDGQIGAEPTLEAYIEDLV
ALFREVRRTLRDDGTLWLNIGDGFTSGGRTWRAPDKKNKGRAMEYRAPTPDGLKPKDLIGVPWRLAIALQADGWYLRADI
IWQKPNCQPESVKDRPTRSHEYVFLLTKSERYYYNSDAVKELTADGKHTKNRRSVWAINTEPYGGNHFAVFPTELVRLCM
EAGSKKEEVVLDPFFGSGTVGQVARDNGRKFVGIELSPDYAELARKRLASKSNSRKLQR

Sequences:

>Translated_299_residues
MLLIVNENEPDTSTHTDEPIQEPARGRLVVGDARKVLAGMADKSFQCCITSPPYWGLRDYGVDGQIGAEPTLEAYIEDLV
ALFREVRRTLRDDGTLWLNIGDGFTSGGRTWRAPDKKNKGRAMEYRAPTPDGLKPKDLIGVPWRLAIALQADGWYLRADI
IWQKPNCQPESVKDRPTRSHEYVFLLTKSERYYYNSDAVKELTADGKHTKNRRSVWAINTEPYGGNHFAVFPTELVRLCM
EAGSKKEEVVLDPFFGSGTVGQVARDNGRKFVGIELSPDYAELARKRLASKSNSRKLQR
>Mature_299_residues
MLLIVNENEPDTSTHTDEPIQEPARGRLVVGDARKVLAGMADKSFQCCITSPPYWGLRDYGVDGQIGAEPTLEAYIEDLV
ALFREVRRTLRDDGTLWLNIGDGFTSGGRTWRAPDKKNKGRAMEYRAPTPDGLKPKDLIGVPWRLAIALQADGWYLRADI
IWQKPNCQPESVKDRPTRSHEYVFLLTKSERYYYNSDAVKELTADGKHTKNRRSVWAINTEPYGGNHFAVFPTELVRLCM
EAGSKKEEVVLDPFFGSGTVGQVARDNGRKFVGIELSPDYAELARKRLASKSNSRKLQR

Specific function: This methylase recognizes the double-stranded sequence CCCGGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the SmaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 33721; Mature: 33721

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLIVNENEPDTSTHTDEPIQEPARGRLVVGDARKVLAGMADKSFQCCITSPPYWGLRDY
CEEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHCCCCCCEEEECCCCCCCCEEC
GVDGQIGAEPTLEAYIEDLVALFREVRRTLRDDGTLWLNIGDGFTSGGRTWRAPDKKNKG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCCCCC
RAMEYRAPTPDGLKPKDLIGVPWRLAIALQADGWYLRADIIWQKPNCQPESVKDRPTRSH
CEEEECCCCCCCCCCHHEECCCEEEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCC
EYVFLLTKSERYYYNSDAVKELTADGKHTKNRRSVWAINTEPYGGNHFAVFPTELVRLCM
CEEEEEEECCCEEECCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEECHHHHHHHHH
EAGSKKEEVVLDPFFGSGTVGQVARDNGRKFVGIELSPDYAELARKRLASKSNSRKLQR
HCCCCCCEEEEECCCCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLLIVNENEPDTSTHTDEPIQEPARGRLVVGDARKVLAGMADKSFQCCITSPPYWGLRDY
CEEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHCCCCCCEEEECCCCCCCCEEC
GVDGQIGAEPTLEAYIEDLVALFREVRRTLRDDGTLWLNIGDGFTSGGRTWRAPDKKNKG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCCCCC
RAMEYRAPTPDGLKPKDLIGVPWRLAIALQADGWYLRADIIWQKPNCQPESVKDRPTRSH
CEEEECCCCCCCCCCHHEECCCEEEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCC
EYVFLLTKSERYYYNSDAVKELTADGKHTKNRRSVWAINTEPYGGNHFAVFPTELVRLCM
CEEEEEEECCCEEECCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEECHHHHHHHHH
EAGSKKEEVVLDPFFGSGTVGQVARDNGRKFVGIELSPDYAELARKRLASKSNSRKLQR
HCCCCCCEEEEECCCCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2690008; 2251121 [H]