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The map label for this gene is paaF [C]

Identifier: 78046474

GI number: 78046474

Start: 1048334

End: 1049161

Strand: Reverse

Name: paaF [C]

Synonym: XCV0918

Alternate gene names: 78046474

Gene position: 1049161-1048334 (Counterclockwise)

Preceding gene: 78046475

Following gene: 78046473

Centisome position: 20.26

GC content: 63.77

Gene sequence:

>828_bases
TTGAACGAGCATGACGTGGTATCGGTTCGCATCGAAAACCGCATCGCCTGGGTGAAGTTCGCGCGCCCCGACAAGCGCAA
CGCGATGAGCCCGGCGCTCAACCGCCGCATGATGGACGTGCTGGACGAGCTGGAGTTCGACGACAACGTCGGCGTGCTGG
TGCTCGGCGGCGAAGGCACCGCCTGGTCGGCGGGCATGGACCTGAAGGAATATTTCCGCGAAACCGAAGCGCAGGGCCTG
CGTGGCGTGCGGCGCTCGCAGCGCGAATCGTATGGCTGGTTCCGTCGCCTGCGCTGGTATCAGAAGCCGACCATCGCCAT
GGTCAACGGCTGGTGTTTCGGCGGCGGCTTCGGCCCGCTGTTTGCCTGCGACCTGGCGATTGCCGCCGATGAGGCGCAGT
TTGGCCTGTCGGAAATCAACTGGGGCATCCTGCCCGGCGGTGGCGTCACCAAGGTCGCGGTGGAACTGCTGTCGATGCGC
GATGCGATGTGGATGACGCTGACCGGCGAAATGGTGGATGGCAAGAAGGCTGCCGAATGGCGCCTGGTCAACGAAAGCGT
GCCGCTGGAACGCCTGGAGGCACGCACCCGCGAAGTGGCCGAACTGTTGCTGCGCAAGAACCCGGTCGCGCTCAAGTACG
CCAAGGATGCGGTGCGCCGCGTCGGCACCATGACCTACGACGAAGCCGAAGATTACCTGGTGCGCATGCAGGAAGCGGCC
AATTCGTTCGACAACAACGCCCGCAAGGAAGGCATCCGTCAGTTCATCGACGAGAAGAGCTACAAGCCCGGCCTGGGCGA
GTACGACCTCAGCAAACACCGAGCGTAA

Upstream 100 bases:

>100_bases
CGCTGCACTGGAGCGCGCCGCCGCGCCAGACACCGCGTTGGCCGAAGCACTCTGAGCCCATCAACACACCAGCACTCTCA
CGACTCGAAGGAGACGCCGC

Downstream 100 bases:

>100_bases
GCGCGTCCTACGGCGTCCGAAGCGGTGCGCGTTGTGCGTGCATCGCTGGCGCTGCCAGTGGTTTTCTGCACCGAGGAGGG
AGTATGGAAGCCTTATTCGA

Product: p-hydroxycinnamoyl CoA hydratase/lyase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MNEHDVVSVRIENRIAWVKFARPDKRNAMSPALNRRMMDVLDELEFDDNVGVLVLGGEGTAWSAGMDLKEYFRETEAQGL
RGVRRSQRESYGWFRRLRWYQKPTIAMVNGWCFGGGFGPLFACDLAIAADEAQFGLSEINWGILPGGGVTKVAVELLSMR
DAMWMTLTGEMVDGKKAAEWRLVNESVPLERLEARTREVAELLLRKNPVALKYAKDAVRRVGTMTYDEAEDYLVRMQEAA
NSFDNNARKEGIRQFIDEKSYKPGLGEYDLSKHRA

Sequences:

>Translated_275_residues
MNEHDVVSVRIENRIAWVKFARPDKRNAMSPALNRRMMDVLDELEFDDNVGVLVLGGEGTAWSAGMDLKEYFRETEAQGL
RGVRRSQRESYGWFRRLRWYQKPTIAMVNGWCFGGGFGPLFACDLAIAADEAQFGLSEINWGILPGGGVTKVAVELLSMR
DAMWMTLTGEMVDGKKAAEWRLVNESVPLERLEARTREVAELLLRKNPVALKYAKDAVRRVGTMTYDEAEDYLVRMQEAA
NSFDNNARKEGIRQFIDEKSYKPGLGEYDLSKHRA
>Mature_275_residues
MNEHDVVSVRIENRIAWVKFARPDKRNAMSPALNRRMMDVLDELEFDDNVGVLVLGGEGTAWSAGMDLKEYFRETEAQGL
RGVRRSQRESYGWFRRLRWYQKPTIAMVNGWCFGGGFGPLFACDLAIAADEAQFGLSEINWGILPGGGVTKVAVELLSMR
DAMWMTLTGEMVDGKKAAEWRLVNESVPLERLEARTREVAELLLRKNPVALKYAKDAVRRVGTMTYDEAEDYLVRMQEAA
NSFDNNARKEGIRQFIDEKSYKPGLGEYDLSKHRA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=29.5546558704453, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI31542718, Length=270, Percent_Identity=24.8148148148148, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI70995211, Length=154, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI20127408, Length=214, Percent_Identity=26.1682242990654, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI68989263, Length=192, Percent_Identity=27.0833333333333, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=29.3650793650794, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788597, Length=208, Percent_Identity=32.2115384615385, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI221142681, Length=221, Percent_Identity=30.7692307692308, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI87082183, Length=269, Percent_Identity=26.3940520446097, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788682, Length=205, Percent_Identity=25.8536585365854, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=25.7936507936508, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790281, Length=185, Percent_Identity=28.1081081081081, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=30.5555555555556, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=212, Percent_Identity=29.7169811320755, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=27.0161290322581, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=191, Percent_Identity=29.3193717277487, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=166, Percent_Identity=31.3253012048193, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17508951, Length=166, Percent_Identity=31.3253012048193, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17508953, Length=166, Percent_Identity=31.3253012048193, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=210, Percent_Identity=25.7142857142857, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=219, Percent_Identity=26.4840182648402, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17536985, Length=148, Percent_Identity=29.7297297297297, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=29.4354838709677, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=29.4354838709677, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=27.6, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=23.8461538461538, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21357171, Length=214, Percent_Identity=25.7009345794392, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24650670, Length=258, Percent_Identity=24.8062015503876, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 31267; Mature: 31267

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEHDVVSVRIENRIAWVKFARPDKRNAMSPALNRRMMDVLDELEFDDNVGVLVLGGEGT
CCCCCEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
AWSAGMDLKEYFRETEAQGLRGVRRSQRESYGWFRRLRWYQKPTIAMVNGWCFGGGFGPL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECEEECCCCHHH
FACDLAIAADEAQFGLSEINWGILPGGGVTKVAVELLSMRDAMWMTLTGEMVDGKKAAEW
HHHHHHHCCCHHHCCHHHCCCCEECCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHE
RLVNESVPLERLEARTREVAELLLRKNPVALKYAKDAVRRVGTMTYDEAEDYLVRMQEAA
EEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NSFDNNARKEGIRQFIDEKSYKPGLGEYDLSKHRA
HHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MNEHDVVSVRIENRIAWVKFARPDKRNAMSPALNRRMMDVLDELEFDDNVGVLVLGGEGT
CCCCCEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
AWSAGMDLKEYFRETEAQGLRGVRRSQRESYGWFRRLRWYQKPTIAMVNGWCFGGGFGPL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECEEECCCCHHH
FACDLAIAADEAQFGLSEINWGILPGGGVTKVAVELLSMRDAMWMTLTGEMVDGKKAAEW
HHHHHHHCCCHHHCCHHHCCCCEECCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHE
RLVNESVPLERLEARTREVAELLLRKNPVALKYAKDAVRRVGTMTYDEAEDYLVRMQEAA
EEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NSFDNNARKEGIRQFIDEKSYKPGLGEYDLSKHRA
HHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA