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The map label for this gene is ksgA [H]

Identifier: 78046456

GI number: 78046456

Start: 1028290

End: 1029078

Strand: Reverse

Name: ksgA [H]

Synonym: XCV0900

Alternate gene names: 78046456

Gene position: 1029078-1028290 (Counterclockwise)

Preceding gene: 78046457

Following gene: 78046455

Centisome position: 19.87

GC content: 66.03

Gene sequence:

>789_bases
ATGAATTCTTCTTTCAGCGCACCGGCCAAGAAGTCGCTTGGCCAGCACTTTCTTGCCGACCGGTATTACATCGACCGCAT
CGTGCAGGCGGTAGACCCGCGCGCCGGCCAGCATCTGGTCGAGATCGGCCCGGGCCAGGGTGCCATCACCTTTCCGCTGC
TGCGCAGGCATGGCGCGCTGACCGTGATCGAATTCGATCGCGACCTGATCGCGCCGTTGACCGAAGTGGCGGCACCGATC
GGCGCGCTGAGCATCATCCACCGCGATGTGCTCAGCGTGGATTTCACTGCACTGGCCAACGGCACGCCGATCCGCCTGGT
CGGCAACCTGCCCTACAACATCTCCTCGCCGATCCTGTTCCATGCGCTGGACCATGCCGCTGCCGTGGCCGACATGCATT
TCATGCTGCAAAAGGAAGTGGTCGACCGCATGGCCGCCGGCCCCGGCAGCAAGGTGTACGGACGGCTCAGCGTGATGCTG
CAGGCGTATTGCGAGGTGACTGCGCTGTTCGTGGTGCCGCCGGGCGCATTCCGGCCGCCGCCGAAGGTGGACTCGGCCGT
CGTGCGGCTGGTGCCGCGCGACCCGGCCACGGTGCAGATCAACGACCGCCGCCGCTTCGCCGATGTGGTGCGCGCCGGGT
TCGGGCAGCGCCGCAAGACCCTGCGCAATGCCTTGTCCACCATTTGCGAACCGGCCCATTTCGACGCCGCGCAGGTGCGC
CCGGATGCACGCGCCGAACAACTGGAGGTCGCGGATTTCATCCGGCTGGCCAATGTCGAGCTGGCTTGA

Upstream 100 bases:

>100_bases
CGGCGCGGGCATGCCGCATGGCGCACGCCGCCGATCCGCCAGGCCAGGCGGGCACGCGAACGACACCGCGCGCAGGCGCG
GCCTCCGTTAAACTGCGCAG

Downstream 100 bases:

>100_bases
GCGCCGCTCCCACACCGTTTTATTTAGACTTTCGCCATGCAAGATGATCCGCGCTACCGCGTCGAGGTCGAGGTATCGCC
CCGCTTCCTCGCCCATCAAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MNSSFSAPAKKSLGQHFLADRYYIDRIVQAVDPRAGQHLVEIGPGQGAITFPLLRRHGALTVIEFDRDLIAPLTEVAAPI
GALSIIHRDVLSVDFTALANGTPIRLVGNLPYNISSPILFHALDHAAAVADMHFMLQKEVVDRMAAGPGSKVYGRLSVML
QAYCEVTALFVVPPGAFRPPPKVDSAVVRLVPRDPATVQINDRRRFADVVRAGFGQRRKTLRNALSTICEPAHFDAAQVR
PDARAEQLEVADFIRLANVELA

Sequences:

>Translated_262_residues
MNSSFSAPAKKSLGQHFLADRYYIDRIVQAVDPRAGQHLVEIGPGQGAITFPLLRRHGALTVIEFDRDLIAPLTEVAAPI
GALSIIHRDVLSVDFTALANGTPIRLVGNLPYNISSPILFHALDHAAAVADMHFMLQKEVVDRMAAGPGSKVYGRLSVML
QAYCEVTALFVVPPGAFRPPPKVDSAVVRLVPRDPATVQINDRRRFADVVRAGFGQRRKTLRNALSTICEPAHFDAAQVR
PDARAEQLEVADFIRLANVELA
>Mature_262_residues
MNSSFSAPAKKSLGQHFLADRYYIDRIVQAVDPRAGQHLVEIGPGQGAITFPLLRRHGALTVIEFDRDLIAPLTEVAAPI
GALSIIHRDVLSVDFTALANGTPIRLVGNLPYNISSPILFHALDHAAAVADMHFMLQKEVVDRMAAGPGSKVYGRLSVML
QAYCEVTALFVVPPGAFRPPPKVDSAVVRLVPRDPATVQINDRRRFADVVRAGFGQRRKTLRNALSTICEPAHFDAAQVR
PDARAEQLEVADFIRLANVELA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=275, Percent_Identity=32.3636363636364, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI156415992, Length=280, Percent_Identity=31.4285714285714, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1786236, Length=252, Percent_Identity=49.2063492063492, Blast_Score=243, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI25146882, Length=193, Percent_Identity=35.7512953367876, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI25141369, Length=246, Percent_Identity=29.2682926829268, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324989, Length=212, Percent_Identity=30.188679245283, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357273, Length=281, Percent_Identity=33.8078291814947, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21358017, Length=217, Percent_Identity=34.5622119815668, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 28585; Mature: 28585

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSFSAPAKKSLGQHFLADRYYIDRIVQAVDPRAGQHLVEIGPGQGAITFPLLRRHGAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEHHHHHCCCCE
TVIEFDRDLIAPLTEVAAPIGALSIIHRDVLSVDFTALANGTPIRLVGNLPYNISSPILF
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHH
HALDHAAAVADMHFMLQKEVVDRMAAGPGSKVYGRLSVMLQAYCEVTALFVVPPGAFRPP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCC
PKVDSAVVRLVPRDPATVQINDRRRFADVVRAGFGQRRKTLRNALSTICEPAHFDAAQVR
CCCCCHHEEEECCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHCC
PDARAEQLEVADFIRLANVELA
CCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNSSFSAPAKKSLGQHFLADRYYIDRIVQAVDPRAGQHLVEIGPGQGAITFPLLRRHGAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEHHHHHCCCCE
TVIEFDRDLIAPLTEVAAPIGALSIIHRDVLSVDFTALANGTPIRLVGNLPYNISSPILF
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHH
HALDHAAAVADMHFMLQKEVVDRMAAGPGSKVYGRLSVMLQAYCEVTALFVVPPGAFRPP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCC
PKVDSAVVRLVPRDPATVQINDRRRFADVVRAGFGQRRKTLRNALSTICEPAHFDAAQVR
CCCCCHHEEEECCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHCC
PDARAEQLEVADFIRLANVELA
CCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]