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The map label for this gene is metF [H]

Identifier: 78046398

GI number: 78046398

Start: 960921

End: 961748

Strand: Reverse

Name: metF [H]

Synonym: XCV0842

Alternate gene names: 78046398

Gene position: 961748-960921 (Counterclockwise)

Preceding gene: 78046399

Following gene: 78046397

Centisome position: 18.57

GC content: 64.61

Gene sequence:

>828_bases
ATGACGCATCTCAGTTTCGAGTTCTACCCGCCCAAGACCGATGATCAGCGCGCGCAACTGGACCGCACTGCAGCCAGGTT
GAAAGGCTACGCGCCGGAGTACGTGTCGTGCACCTTCGGCGCCGGCGGCTCCACGCTCAGCTACACCTCCGAAACGGTAC
GCCACCTCAAGCAGAAGCATGGCTTCGAAGCGGCGCCGCACCTGTCCTGCGTCGGCGGCAGCCGGCAGGAAATCCGCGAG
CTGCTCAAGCTGTATCGCGCCATCGGTTGCACCCGCATCGTTGCGCTGCGTGGCGACCTGCCGTCGGGCATGGGCCATCC
CGGGGACCTGCGTTACGCCTCGGACCTGATCGCCTTCATCCGCGCCGAGCACGGCGCCGCGTTCCGGATCGAGGTCGGCG
CCTATCCGGAAACCCATCCGCAGGCGGCCGACGCGCTCAGCGACCTGCGCTATTTCAAGGCCAAGGTCGATGCCGGCGCC
GATGCGGCCATTACCCAGTATTTCTACAACGCCGATGCCTACTTTCATTTCCGCGACGCAGTGCAGCGGATGGGCGTGGA
GATACCGATCATTCCCGGCATCATGCCGATCTCCAATTTCTCGCAGCTGCGGCGGTTTTCCGAGCAATGCGGCGCAGAGA
TTCCACGCTGGATCGGCAAGAAGATGCAGTCCTACGGCGACGACGCCGACGCAGTGCGCGCCTTCGGCGCAGAGGTCGTG
GCGGCCTTGTGCCAGCGCCTGATCGCAGGTGGCGCGCCGGCATTGCATTTCTACACCCTCAATCTGGCCAAGCCGACCAC
GCAGGTGTTGCAGCTGCTCGGACGCTGA

Upstream 100 bases:

>100_bases
ACCGGCCCTGCGACAGGGCGCATCGGAACCGTCGCAACCTGATATGCATTCATCGCGATGAAGCGATATTATCGCCAGAC
GATCAGCCGGAACCGCCGCG

Downstream 100 bases:

>100_bases
GCACCTGCTTCGCATTCCAGTCGTCGCCCTCGCACACCCGCGCGCATGTATGTCGCCAGCTCACGCTTACCCGGCTACGC
TGCGCCGATGCATCGCCTTC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTHLSFEFYPPKTDDQRAQLDRTAARLKGYAPEYVSCTFGAGGSTLSYTSETVRHLKQKHGFEAAPHLSCVGGSRQEIRE
LLKLYRAIGCTRIVALRGDLPSGMGHPGDLRYASDLIAFIRAEHGAAFRIEVGAYPETHPQAADALSDLRYFKAKVDAGA
DAAITQYFYNADAYFHFRDAVQRMGVEIPIIPGIMPISNFSQLRRFSEQCGAEIPRWIGKKMQSYGDDADAVRAFGAEVV
AALCQRLIAGGAPALHFYTLNLAKPTTQVLQLLGR

Sequences:

>Translated_275_residues
MTHLSFEFYPPKTDDQRAQLDRTAARLKGYAPEYVSCTFGAGGSTLSYTSETVRHLKQKHGFEAAPHLSCVGGSRQEIRE
LLKLYRAIGCTRIVALRGDLPSGMGHPGDLRYASDLIAFIRAEHGAAFRIEVGAYPETHPQAADALSDLRYFKAKVDAGA
DAAITQYFYNADAYFHFRDAVQRMGVEIPIIPGIMPISNFSQLRRFSEQCGAEIPRWIGKKMQSYGDDADAVRAFGAEVV
AALCQRLIAGGAPALHFYTLNLAKPTTQVLQLLGR
>Mature_274_residues
THLSFEFYPPKTDDQRAQLDRTAARLKGYAPEYVSCTFGAGGSTLSYTSETVRHLKQKHGFEAAPHLSCVGGSRQEIREL
LKLYRAIGCTRIVALRGDLPSGMGHPGDLRYASDLIAFIRAEHGAAFRIEVGAYPETHPQAADALSDLRYFKAKVDAGAD
AAITQYFYNADAYFHFRDAVQRMGVEIPIIPGIMPISNFSQLRRFSEQCGAEIPRWIGKKMQSYGDDADAVRAFGAEVVA
ALCQRLIAGGAPALHFYTLNLAKPTTQVLQLLGR

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=279, Percent_Identity=37.6344086021505, Blast_Score=178, Evalue=4e-45,
Organism=Escherichia coli, GI1790377, Length=272, Percent_Identity=39.7058823529412, Blast_Score=222, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=33.0935251798561, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=33.0935251798561, Blast_Score=160, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6321313, Length=279, Percent_Identity=33.6917562724014, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6325234, Length=284, Percent_Identity=32.0422535211268, Blast_Score=149, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24662658, Length=222, Percent_Identity=25.6756756756757, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30262; Mature: 30131

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHLSFEFYPPKTDDQRAQLDRTAARLKGYAPEYVSCTFGAGGSTLSYTSETVRHLKQKH
CCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
GFEAAPHLSCVGGSRQEIRELLKLYRAIGCTRIVALRGDLPSGMGHPGDLRYASDLIAFI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHH
RAEHGAAFRIEVGAYPETHPQAADALSDLRYFKAKVDAGADAAITQYFYNADAYFHFRDA
HHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHH
VQRMGVEIPIIPGIMPISNFSQLRRFSEQCGAEIPRWIGKKMQSYGDDADAVRAFGAEVV
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AALCQRLIAGGAPALHFYTLNLAKPTTQVLQLLGR
HHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure 
THLSFEFYPPKTDDQRAQLDRTAARLKGYAPEYVSCTFGAGGSTLSYTSETVRHLKQKH
CCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
GFEAAPHLSCVGGSRQEIRELLKLYRAIGCTRIVALRGDLPSGMGHPGDLRYASDLIAFI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHH
RAEHGAAFRIEVGAYPETHPQAADALSDLRYFKAKVDAGADAAITQYFYNADAYFHFRDA
HHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHH
VQRMGVEIPIIPGIMPISNFSQLRRFSEQCGAEIPRWIGKKMQSYGDDADAVRAFGAEVV
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AALCQRLIAGGAPALHFYTLNLAKPTTQVLQLLGR
HHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]