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The map label for this gene is dsbA [H]

Identifier: 78046335

GI number: 78046335

Start: 891707

End: 892357

Strand: Reverse

Name: dsbA [H]

Synonym: XCV0779

Alternate gene names: 78046335

Gene position: 892357-891707 (Counterclockwise)

Preceding gene: 78046336

Following gene: 78046334

Centisome position: 17.23

GC content: 65.28

Gene sequence:

>651_bases
ATGAAATTGCTTTCCCGTCTGCCCCTGCTGTTGATGTTCCTGTTGCCGCTGGTGGCCTGCGCCGCCGACAAGAAAGCCGC
GCCGGTCGAAGGCGAGGACTACACCCTGATCGACGGCGGCCAGCCGTACGCCCCGCTGGCCGGCAAGGTCGAAGTGACCG
AAGTCTTCGGCTACACCTGCCCGCATTGCGCACATTTCGAACCAGTGCTGGAAGCCTGGGTGGCCAAGCAGCCGGCATAC
GTACGCTTCACCCCGGTACCGGCCGCCTTCGGTGGCTTCTGGGACGCGTTTGCGCGCGCCTACTTCGCCGCCGACATCCT
GGGCGTGGCCAAGCGCAGCCATCGCGCCATGTTCGACGCCATCCATGAAAAGCAGACCGTGCCCACCCAGAACGTGGCGC
CCGAAGAACTGGCCGCGTTCTATGCCGGTTACGGAATCCCGCAGCAGCGCTTCATCGAGACCTACAAGAGCGAGGCGGTG
GAGGCAAAGCTCAAGGCCGCGCGCGAGTTCGCGCTGCGCAGCAAGATTCCCGGCACGCCGGCCATCATCGTCAACGGCCG
CTACCTGATCGGCGCACGCAATTACCCGGACATGTTGCGCATTGCCGACTACCTGATCGCCCGCGAGCACGCCGCGCCGG
CCAAGCGCTGA

Upstream 100 bases:

>100_bases
TTCGTGATCTGATTCCTGCCGTCGCCACGCCGCTTCACGACGCCGGTCCCGCGACCGGCGTCGTCGTTTACCGCTCTCCC
CTCGCCCCCACGGAGCCCGC

Downstream 100 bases:

>100_bases
ACCGCATAACCGCCAGCGACGGCCGCGCGTGGCATCATGCGCCCCGCGGCCGTCCGGCCCTTTTCTCGCCCCACGCTGGA
GATCCAATCCGATGAAGACA

Product: putative disulfide oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKLLSRLPLLLMFLLPLVACAADKKAAPVEGEDYTLIDGGQPYAPLAGKVEVTEVFGYTCPHCAHFEPVLEAWVAKQPAY
VRFTPVPAAFGGFWDAFARAYFAADILGVAKRSHRAMFDAIHEKQTVPTQNVAPEELAAFYAGYGIPQQRFIETYKSEAV
EAKLKAAREFALRSKIPGTPAIIVNGRYLIGARNYPDMLRIADYLIAREHAAPAKR

Sequences:

>Translated_216_residues
MKLLSRLPLLLMFLLPLVACAADKKAAPVEGEDYTLIDGGQPYAPLAGKVEVTEVFGYTCPHCAHFEPVLEAWVAKQPAY
VRFTPVPAAFGGFWDAFARAYFAADILGVAKRSHRAMFDAIHEKQTVPTQNVAPEELAAFYAGYGIPQQRFIETYKSEAV
EAKLKAAREFALRSKIPGTPAIIVNGRYLIGARNYPDMLRIADYLIAREHAAPAKR
>Mature_216_residues
MKLLSRLPLLLMFLLPLVACAADKKAAPVEGEDYTLIDGGQPYAPLAGKVEVTEVFGYTCPHCAHFEPVLEAWVAKQPAY
VRFTPVPAAFGGFWDAFARAYFAADILGVAKRSHRAMFDAIHEKQTVPTQNVAPEELAAFYAGYGIPQQRFIETYKSEAV
EAKLKAAREFALRSKIPGTPAIIVNGRYLIGARNYPDMLRIADYLIAREHAAPAKR

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=167, Percent_Identity=25.748502994012, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23842; Mature: 23842

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLSRLPLLLMFLLPLVACAADKKAAPVEGEDYTLIDGGQPYAPLAGKVEVTEVFGYTC
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCEEHHHHHCCCC
PHCAHFEPVLEAWVAKQPAYVRFTPVPAAFGGFWDAFARAYFAADILGVAKRSHRAMFDA
CCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHEKQTVPTQNVAPEELAAFYAGYGIPQQRFIETYKSEAVEAKLKAAREFALRSKIPGTP
HHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AIIVNGRYLIGARNYPDMLRIADYLIAREHAAPAKR
EEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLLSRLPLLLMFLLPLVACAADKKAAPVEGEDYTLIDGGQPYAPLAGKVEVTEVFGYTC
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCEEHHHHHCCCC
PHCAHFEPVLEAWVAKQPAYVRFTPVPAAFGGFWDAFARAYFAADILGVAKRSHRAMFDA
CCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHEKQTVPTQNVAPEELAAFYAGYGIPQQRFIETYKSEAVEAKLKAAREFALRSKIPGTP
HHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AIIVNGRYLIGARNYPDMLRIADYLIAREHAAPAKR
EEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10051655 [H]