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The map label for this gene is pobB [H]

Identifier: 78045932

GI number: 78045932

Start: 429684

End: 430667

Strand: Reverse

Name: pobB [H]

Synonym: XCV0376

Alternate gene names: 78045932

Gene position: 430667-429684 (Counterclockwise)

Preceding gene: 78045933

Following gene: 78045931

Centisome position: 8.32

GC content: 67.28

Gene sequence:

>984_bases
ATGCGTAAAGACACCCAGTGGCATCGTGCCCGCGTCGTCAGCATTGCCGACGCCTGCCCGGGCGTGCGCGAAATCGTGCT
CGATCCCGGCGCCGCTGCACGCAGCTTCGAGGTGGGCAGCCATGTGGATTTTCGTGTGCAGCTGCATGGTCGCGACGATG
TGCGCTCGTATTCGCTGGTGGGCGAACCGCGTGCCGACGGGTACTACCAGATCGCAGTGCGGCAAATGCCCGACAGCCGC
GGCGGCTCGCTGCATATGTGGACGCTTGCGCCGGGCGATGTGGTGGAGATGTCGCCGCCCAGCAACAACTTCGCGCTGGA
TGAAAGCGGCGAGGAAATCCTGCTGATTGCCGGCGGCATCGGCATCACCCCGATCGTCGGCATGGCGCAGCGGCTGGCGC
GTGGCCACCGCGCGTTCCGTCTGCTGTACGCCGGCCGCTCGCGCAGCGCAATGGCGTATGTCGACACGCTGGAAGCACTG
CTAGGCGAACGTTTGCAACTGCAATGCGACGACAGCGCCGGCCCGCCGGATCTGGCTGCCGAACTGGCGCGCCTGTCGCC
CAATGCCGAGGTGTATGTGTGCGGGCCGCTGGGCATGCTCGAGGCGGTACGCCAGCACTGGCACGCCGCCGGCCGGCCGC
GTGCGCGCCTGCACTTCGAAACCTTCGGCAATAGCGGGCGGGTGCCGGCGCAGGCGTTCGTGGTGAAATTGCCGGGGCTG
GGCCTGGAAGTGCCGGTCGCTGAAAACGAATCGATGCTGGACGCACTGGCCGACGCCGGGGTGGAGCTGATCGCCGAGTG
CCGCCGTGGCGAATGCGGGCTGTGCGCGGTGGACGTGCTGGACAGCTCCGCCGATATCGATCACCGCGACGTGTTCTTCA
GCGACGAGCAGCGCCGGGAGAATCGCAAGCTCTGTGCCTGCGTGTCGCGTGCGGTAGGCGGCAGCATCAGCATCGACACC
GGCTATCGCAGCAAGCTGGGCTGA

Upstream 100 bases:

>100_bases
GCACTTCGCATGCGGCCAGCCATCTCATCGCTCACAGCGCCGCATTGCGGCGCCGTCACAGCCGGCGCCGGCCGGCAGAC
CTCATTGCCCGGAGCCCTCC

Downstream 100 bases:

>100_bases
GCCGGCATCCGGCCGGCTGCGCATCGTCCGGCGGTTGCGGTCGATACTGGGCTTCAGCGAGCAACGCGTTTGCGGCGCCC
GCGTTTCATCGGCACGACCC

Product: putative vanillate o-demethylase oxidoreductase

Products: NA

Alternate protein names: 4-carboxydiphenyl ether; phenoxybenzoate dioxygenase subunit beta [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MRKDTQWHRARVVSIADACPGVREIVLDPGAAARSFEVGSHVDFRVQLHGRDDVRSYSLVGEPRADGYYQIAVRQMPDSR
GGSLHMWTLAPGDVVEMSPPSNNFALDESGEEILLIAGGIGITPIVGMAQRLARGHRAFRLLYAGRSRSAMAYVDTLEAL
LGERLQLQCDDSAGPPDLAAELARLSPNAEVYVCGPLGMLEAVRQHWHAAGRPRARLHFETFGNSGRVPAQAFVVKLPGL
GLEVPVAENESMLDALADAGVELIAECRRGECGLCAVDVLDSSADIDHRDVFFSDEQRRENRKLCACVSRAVGGSISIDT
GYRSKLG

Sequences:

>Translated_327_residues
MRKDTQWHRARVVSIADACPGVREIVLDPGAAARSFEVGSHVDFRVQLHGRDDVRSYSLVGEPRADGYYQIAVRQMPDSR
GGSLHMWTLAPGDVVEMSPPSNNFALDESGEEILLIAGGIGITPIVGMAQRLARGHRAFRLLYAGRSRSAMAYVDTLEAL
LGERLQLQCDDSAGPPDLAAELARLSPNAEVYVCGPLGMLEAVRQHWHAAGRPRARLHFETFGNSGRVPAQAFVVKLPGL
GLEVPVAENESMLDALADAGVELIAECRRGECGLCAVDVLDSSADIDHRDVFFSDEQRRENRKLCACVSRAVGGSISIDT
GYRSKLG
>Mature_327_residues
MRKDTQWHRARVVSIADACPGVREIVLDPGAAARSFEVGSHVDFRVQLHGRDDVRSYSLVGEPRADGYYQIAVRQMPDSR
GGSLHMWTLAPGDVVEMSPPSNNFALDESGEEILLIAGGIGITPIVGMAQRLARGHRAFRLLYAGRSRSAMAYVDTLEAL
LGERLQLQCDDSAGPPDLAAELARLSPNAEVYVCGPLGMLEAVRQHWHAAGRPRARLHFETFGNSGRVPAQAFVVKLPGL
GLEVPVAENESMLDALADAGVELIAECRRGECGLCAVDVLDSSADIDHRDVFFSDEQRRENRKLCACVSRAVGGSISIDT
GYRSKLG

Specific function: Degrades exclusively diarylether compounds having carboxyl groups in the 3- or 4-position. Yields a hemiacetal that spontaneously hydrolyzes to phenol and protocatechuate [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=326, Percent_Identity=34.6625766871166, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1787658, Length=288, Percent_Identity=26.3888888888889, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787098, Length=275, Percent_Identity=25.8181818181818, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788903, Length=240, Percent_Identity=26.25, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 35433; Mature: 35433

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKDTQWHRARVVSIADACPGVREIVLDPGAAARSFEVGSHVDFRVQLHGRDDVRSYSLV
CCCCCCHHHHEEEEHHHCCCCCCEEEECCCCCCCEEECCCCEEEEEEECCCCCCCEEEEE
GEPRADGYYQIAVRQMPDSRGGSLHMWTLAPGDVVEMSPPSNNFALDESGEEILLIAGGI
CCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCEEEECCCCCCEEECCCCCEEEEEECCC
GITPIVGMAQRLARGHRAFRLLYAGRSRSAMAYVDTLEALLGERLQLQCDDSAGPPDLAA
CHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
ELARLSPNAEVYVCGPLGMLEAVRQHWHAAGRPRARLHFETFGNSGRVPAQAFVVKLPGL
HHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEECCCC
GLEVPVAENESMLDALADAGVELIAECRRGECGLCAVDVLDSSADIDHRDVFFSDEQRRE
CEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCHHHHH
NRKLCACVSRAVGGSISIDTGYRSKLG
HHHHHHHHHHHCCCEEEECCCCCCCCC
>Mature Secondary Structure
MRKDTQWHRARVVSIADACPGVREIVLDPGAAARSFEVGSHVDFRVQLHGRDDVRSYSLV
CCCCCCHHHHEEEEHHHCCCCCCEEEECCCCCCCEEECCCCEEEEEEECCCCCCCEEEEE
GEPRADGYYQIAVRQMPDSRGGSLHMWTLAPGDVVEMSPPSNNFALDESGEEILLIAGGI
CCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCEEEECCCCCCEEECCCCCEEEEEECCC
GITPIVGMAQRLARGHRAFRLLYAGRSRSAMAYVDTLEALLGERLQLQCDDSAGPPDLAA
CHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
ELARLSPNAEVYVCGPLGMLEAVRQHWHAAGRPRARLHFETFGNSGRVPAQAFVVKLPGL
HHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEECCCC
GLEVPVAENESMLDALADAGVELIAECRRGECGLCAVDVLDSSADIDHRDVFFSDEQRRE
CEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCHHHHH
NRKLCACVSRAVGGSISIDTGYRSKLG
HHHHHHHHHHHCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7710319 [H]