Click here to switch to the map view.

The map label for this gene is ycbU [H]

Identifier: 78045724

GI number: 78045724

Start: 200646

End: 201827

Strand: Reverse

Name: ycbU [H]

Synonym: XCV0168

Alternate gene names: 78045724

Gene position: 201827-200646 (Counterclockwise)

Preceding gene: 162138546

Following gene: 78045719

Centisome position: 3.9

GC content: 68.36

Gene sequence:

>1182_bases
ATGTCATCGCCATCTCTGTCCAGTACGCTTGCTGCGCCCCTCGCGTGGGCCCAGCGTCAGCAGGCGTTCGCGCTGCCTTC
CGGGGTCATCCATCTGGATGCGGCGTCGCGTGGTCCGTTGTTGCGCGCCATGCAGACCGTTGCGCACCAGGCGGTCGATG
CAATGGCCACACCATGGCGGTTGCCGTTCGATACCTGGTTACAGCAGATCGAGCAGGTGCGCGGACTTGCGGCCGCGCTA
TTGGACGGCGATGCCGATGCGGTGGCCATGGTGCCGTCGGCCGCGCACGGCCTGGCCACGGCCGCGCGCAATCTGCCTGT
GCAAGCCGGCGACGCAGTGCTGGTGCTGGAAGGGCAATTTCCGTCCAATCTGTTGATCTGGCAGCGCCGCTGCGCAGAGA
CCGGCGCGCATCTGGTGGCGGTGCCCGCCACCGCTGGCGGCAGCTTGACCGATGCAGTGCTGCAGACCATTGCCGACACG
CCTGCGCTGCGGATTGCCAGCTTGCCGCATGCGTACTGGCTGGACGGGCGAATGCTGGATCTGGACCGCATCAGTGCCGC
CATGCAGGCACGCGGCGCTGCCCTGGTGCTGGACTTGAGCCAGAGCCTTGGGGTGTTGCCACCGGATCTGCCGCGCTGGC
AACCGGATTTCGTGGTCAGCGTCGGGCACAAATGGTTGCTCGGGCCGATGGGATTGGCATGGCTGTGGGTCGCGCCGCAG
TGGCGCACGGACGGGCTGCCGATCGAAGAACACTGGATCGGCCGCGATGCGGGCAGCAGTTGGGAATTCCCGGTGGCCAG
CGCGCCGCGGTATCGCAGCGGTGCACGCCGATTCGATGCGGGCGGGGTTGCCGACCCGTTGCGGGTCGCAACGGCCACGG
TGGCCCTGCAACAACTCACCGCGTGGCAGCCGGCGCGCATCGCAGCGGCGTTGGGCGAGTTGACTGCGCATTGGGACGCC
GCCCTGCAGGCGCGCGGCGTGGGCAGCTGGTGCACGCCCGGACATGCGCCGCATCTCACCGCACTGCAGCCGCCGGCAGC
GGCATTGGATGCGGTGGCAACGGCGCTCGGCGCGAGTGGCGTGATCTGCACGCGTCGGCATGGACGTCTGCGCATCGCGC
CACATTTGAGCGTCACCGACCACGCGTTGTCACAGGTCATTGCAGCGCTGCCGGATGGGTGA

Upstream 100 bases:

>100_bases
TGGCCCAGCAGAGGTGAGAGTGCAGACATCGCCCTATGCTTGGGGAGCGCCCGTGAACGTGCCGTGTGGGCAGCGTGGCA
GCCAGCGGTAAGCTGTGGGC

Downstream 100 bases:

>100_bases
GGCGGCGCGCAGGCATCGCGGGCCAGGCATCTGCAGCGGTACTGGTTGCAACACATGTACGCAGGCCAGCGCGTCATCAG
GCAGCATCGGCAAGGCTCCA

Product: putative secreted protein

Products: hydrogen selenide; @ALAN01.txt*l-alanine!; acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSSPSLSSTLAAPLAWAQRQQAFALPSGVIHLDAASRGPLLRAMQTVAHQAVDAMATPWRLPFDTWLQQIEQVRGLAAAL
LDGDADAVAMVPSAAHGLATAARNLPVQAGDAVLVLEGQFPSNLLIWQRRCAETGAHLVAVPATAGGSLTDAVLQTIADT
PALRIASLPHAYWLDGRMLDLDRISAAMQARGAALVLDLSQSLGVLPPDLPRWQPDFVVSVGHKWLLGPMGLAWLWVAPQ
WRTDGLPIEEHWIGRDAGSSWEFPVASAPRYRSGARRFDAGGVADPLRVATATVALQQLTAWQPARIAAALGELTAHWDA
ALQARGVGSWCTPGHAPHLTALQPPAAALDAVATALGASGVICTRRHGRLRIAPHLSVTDHALSQVIAALPDG

Sequences:

>Translated_393_residues
MSSPSLSSTLAAPLAWAQRQQAFALPSGVIHLDAASRGPLLRAMQTVAHQAVDAMATPWRLPFDTWLQQIEQVRGLAAAL
LDGDADAVAMVPSAAHGLATAARNLPVQAGDAVLVLEGQFPSNLLIWQRRCAETGAHLVAVPATAGGSLTDAVLQTIADT
PALRIASLPHAYWLDGRMLDLDRISAAMQARGAALVLDLSQSLGVLPPDLPRWQPDFVVSVGHKWLLGPMGLAWLWVAPQ
WRTDGLPIEEHWIGRDAGSSWEFPVASAPRYRSGARRFDAGGVADPLRVATATVALQQLTAWQPARIAAALGELTAHWDA
ALQARGVGSWCTPGHAPHLTALQPPAAALDAVATALGASGVICTRRHGRLRIAPHLSVTDHALSQVIAALPDG
>Mature_392_residues
SSPSLSSTLAAPLAWAQRQQAFALPSGVIHLDAASRGPLLRAMQTVAHQAVDAMATPWRLPFDTWLQQIEQVRGLAAALL
DGDADAVAMVPSAAHGLATAARNLPVQAGDAVLVLEGQFPSNLLIWQRRCAETGAHLVAVPATAGGSLTDAVLQTIADTP
ALRIASLPHAYWLDGRMLDLDRISAAMQARGAALVLDLSQSLGVLPPDLPRWQPDFVVSVGHKWLLGPMGLAWLWVAPQW
RTDGLPIEEHWIGRDAGSSWEFPVASAPRYRSGARRFDAGGVADPLRVATATVALQQLTAWQPARIAAALGELTAHWDAA
LQARGVGSWCTPGHAPHLTALQPPAAALDAVATALGASGVICTRRHGRLRIAPHLSVTDHALSQVIAALPDG

Specific function: Cysteine Desulfurases Mobilize The Sulfur From L- Cysteine To Yield L-Alanine, An Essential Step In Sulfur Metabolism For Biosynthesis Of A Variety Of Sulfur-Containing Biomolecules. Component Of The Suf Operon, Which Is Activated And Required Under Speci

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI1787970, Length=297, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.8.1.7 [C]

Molecular weight: Translated: 41437; Mature: 41306

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPSLSSTLAAPLAWAQRQQAFALPSGVIHLDAASRGPLLRAMQTVAHQAVDAMATPWR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
LPFDTWLQQIEQVRGLAAALLDGDADAVAMVPSAAHGLATAARNLPVQAGDAVLVLEGQF
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCC
PSNLLIWQRRCAETGAHLVAVPATAGGSLTDAVLQTIADTPALRIASLPHAYWLDGRMLD
CCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCEEECCCEEEC
LDRISAAMQARGAALVLDLSQSLGVLPPDLPRWQPDFVVSVGHKWLLGPMGLAWLWVAPQ
HHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCCCCEEEECCCCEEECCCCEEEEEECCC
WRTDGLPIEEHWIGRDAGSSWEFPVASAPRYRSGARRFDAGGVADPLRVATATVALQQLT
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AWQPARIAAALGELTAHWDAALQARGVGSWCTPGHAPHLTALQPPAAALDAVATALGASG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
VICTRRHGRLRIAPHLSVTDHALSQVIAALPDG
EEEECCCCEEEEECCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSPSLSSTLAAPLAWAQRQQAFALPSGVIHLDAASRGPLLRAMQTVAHQAVDAMATPWR
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
LPFDTWLQQIEQVRGLAAALLDGDADAVAMVPSAAHGLATAARNLPVQAGDAVLVLEGQF
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCC
PSNLLIWQRRCAETGAHLVAVPATAGGSLTDAVLQTIADTPALRIASLPHAYWLDGRMLD
CCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCEEECCCEEEC
LDRISAAMQARGAALVLDLSQSLGVLPPDLPRWQPDFVVSVGHKWLLGPMGLAWLWVAPQ
HHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCCCCEEEECCCCEEECCCCEEEEEECCC
WRTDGLPIEEHWIGRDAGSSWEFPVASAPRYRSGARRFDAGGVADPLRVATATVALQQLT
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AWQPARIAAALGELTAHWDAALQARGVGSWCTPGHAPHLTALQPPAAALDAVATALGASG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
VICTRRHGRLRIAPHLSVTDHALSQVIAALPDG
EEEECCCCEEEEECCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-selenocysteine; reduced acceptor [C]

Specific reaction: L-selenocysteine + reduced acceptor = hydrogen selenide + @ALAN01.txt*l-alanine! + acceptor [C]

General reaction: Elimination of H2S or RSH [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377; 7704254; 1353026 [H]