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The map label for this gene is cls [H]

Identifier: 78045571

GI number: 78045571

Start: 15368

End: 16828

Strand: Reverse

Name: cls [H]

Synonym: XCV0015

Alternate gene names: 78045571

Gene position: 16828-15368 (Counterclockwise)

Preceding gene: 78045574

Following gene: 78045570

Centisome position: 0.32

GC content: 66.67

Gene sequence:

>1461_bases
ATGCTCGCACTTGCACAGGGCGCGTGGGAGTGGCTGGCCACCATTCCCCACCTCGAAGCGCTCCTCCTGGCCGCCTATGT
GCTGTACCTGCTATGGATCGCCGGCTGGATCGTGCTGCAGAAGCGCGAACCGGTGGCCACGCTGAGCTGGGTGTTGTCGC
TGGCGGCGCTGCCGTACCTGGGGCTGCTGATCTACTACTGGCTGGGCCCGCAAAAGGTCAAACGGCAGCGGCTGCGGCGC
GGGCGCTCGCGCTCGGGCATGGAGAGCTACAGCAGCGTCTGCCCGCCGGATGCCGACTGTACCGAGCTGGCCAAGATCGC
CCAGTCCACCACCGGGCTGGCGCCCAGCAGCGCCACCGAAGTGCACTGGCTGGTGGATGGCGCTGCCACGTATGCCGCCA
TCATCGAAGCGATCCGGGGCGCACGCGACCATATCCATCTGGAGTACTACATCTTCCAGCCCGACCACAGCGGTACCGCG
ATCTGCGCTGCGCTGATGGAACGTGCCCGTGCCGGAGTCAAGGTGCGGCTGCTGATGGATGCGATCGGCTCATCGGCAAT
GACCCGACGCGCCCTGCGCACGCTGCGCGAGGCCGGGGTGGAAACCGCCTGGTTCCATCCCTCGCAATTGCTCAAGCCAT
TCAAGCGGCCGTGGTTGAACCTGCGCACCCACCGCAAGGTGATCGTCATCGATGGCCGCGTGGGTTTCACCGGCGGCATC
AACGTCACCGACGACGAGAACGAACAGGTGCGCAAGCAGGCGTATCGCGATCTGCACGTGCGCCTGCAAGGCCATGTGGT
GCGCAGTCTGCAGCTGGTGTTCCTGGAGGACTGGCTCTATGCCACGGGCCAGGGCCGCGCGGCCTTCCACGGCCAGCAGC
TGTGGCCGGACGATGTGCCCACCCGCGCGCAAGGCACGGTGGATGCGCAAGTGCTGGTGTCCGGCCCGGATTCGTCGTGG
GAAGCGATCCACCGCCTGATGGTCGCCGCCATCCATGAAGCCAAGCATCGCGTGTGGCTGGTCACTCCGTACTTCGTGCC
CGGCGAAGCAGCGCGCATGGCGCTGACCTCCGCCGCACTCGGCGGGCTGGACGTGCGCCTGCTGGTGCCGCGCGTGAGCG
ATTCGCGCCTGGTCACCTACGCGGCGCGTTCGTATTTCGACGAGTTGCTGGAAGCCGGTGTCCGCATCTACGAGTACGGC
CCGCGCATGCTGCATACCAAGGCGCTGCTGGCCGACGACGATGTCTGCATCGTCGGCAGTGCCAATTTCGACAGCCGCAG
CTTCCGGCTGAATTTCGAGTTGTCGATGCTGTTCCGCGACCAGGCCGTGGCAGCAGAGATGGCCGGGCTGATCGGCACCG
ATCTGCAGCAGGCGCAGGAGGTGCAATTCGCGCGGCATCGGCCGTTATGGCGCTCGCGCCTGCCGGAGGCCTTCGCCCGG
CTGTTGTCGCCGCTGCTCTGA

Upstream 100 bases:

>100_bases
AGCATCCTGCGAACAATCACGCGCGATCATCGGAGCTCGAGCAGCACTGCGGCAGTCCAGGCGCCGGTCAGACCGCTGCT
GCGGCTCTTAGAATCCGCGC

Downstream 100 bases:

>100_bases
CCAACGGTGAGGCGCCCACGTGGAACCGCGCCGGGCGCGGCGCTACACTGCCGCCACTCAACGGGGGAGCGCGTCATGCA
CTGGCTGTTTCTGCTTATGG

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MLALAQGAWEWLATIPHLEALLLAAYVLYLLWIAGWIVLQKREPVATLSWVLSLAALPYLGLLIYYWLGPQKVKRQRLRR
GRSRSGMESYSSVCPPDADCTELAKIAQSTTGLAPSSATEVHWLVDGAATYAAIIEAIRGARDHIHLEYYIFQPDHSGTA
ICAALMERARAGVKVRLLMDAIGSSAMTRRALRTLREAGVETAWFHPSQLLKPFKRPWLNLRTHRKVIVIDGRVGFTGGI
NVTDDENEQVRKQAYRDLHVRLQGHVVRSLQLVFLEDWLYATGQGRAAFHGQQLWPDDVPTRAQGTVDAQVLVSGPDSSW
EAIHRLMVAAIHEAKHRVWLVTPYFVPGEAARMALTSAALGGLDVRLLVPRVSDSRLVTYAARSYFDELLEAGVRIYEYG
PRMLHTKALLADDDVCIVGSANFDSRSFRLNFELSMLFRDQAVAAEMAGLIGTDLQQAQEVQFARHRPLWRSRLPEAFAR
LLSPLL

Sequences:

>Translated_486_residues
MLALAQGAWEWLATIPHLEALLLAAYVLYLLWIAGWIVLQKREPVATLSWVLSLAALPYLGLLIYYWLGPQKVKRQRLRR
GRSRSGMESYSSVCPPDADCTELAKIAQSTTGLAPSSATEVHWLVDGAATYAAIIEAIRGARDHIHLEYYIFQPDHSGTA
ICAALMERARAGVKVRLLMDAIGSSAMTRRALRTLREAGVETAWFHPSQLLKPFKRPWLNLRTHRKVIVIDGRVGFTGGI
NVTDDENEQVRKQAYRDLHVRLQGHVVRSLQLVFLEDWLYATGQGRAAFHGQQLWPDDVPTRAQGTVDAQVLVSGPDSSW
EAIHRLMVAAIHEAKHRVWLVTPYFVPGEAARMALTSAALGGLDVRLLVPRVSDSRLVTYAARSYFDELLEAGVRIYEYG
PRMLHTKALLADDDVCIVGSANFDSRSFRLNFELSMLFRDQAVAAEMAGLIGTDLQQAQEVQFARHRPLWRSRLPEAFAR
LLSPLL
>Mature_486_residues
MLALAQGAWEWLATIPHLEALLLAAYVLYLLWIAGWIVLQKREPVATLSWVLSLAALPYLGLLIYYWLGPQKVKRQRLRR
GRSRSGMESYSSVCPPDADCTELAKIAQSTTGLAPSSATEVHWLVDGAATYAAIIEAIRGARDHIHLEYYIFQPDHSGTA
ICAALMERARAGVKVRLLMDAIGSSAMTRRALRTLREAGVETAWFHPSQLLKPFKRPWLNLRTHRKVIVIDGRVGFTGGI
NVTDDENEQVRKQAYRDLHVRLQGHVVRSLQLVFLEDWLYATGQGRAAFHGQQLWPDDVPTRAQGTVDAQVLVSGPDSSW
EAIHRLMVAAIHEAKHRVWLVTPYFVPGEAARMALTSAALGGLDVRLLVPRVSDSRLVTYAARSYFDELLEAGVRIYEYG
PRMLHTKALLADDDVCIVGSANFDSRSFRLNFELSMLFRDQAVAAEMAGLIGTDLQQAQEVQFARHRPLWRSRLPEAFAR
LLSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=485, Percent_Identity=29.6907216494845, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1787007, Length=355, Percent_Identity=29.8591549295775, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 54419; Mature: 54419

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALAQGAWEWLATIPHLEALLLAAYVLYLLWIAGWIVLQKREPVATLSWVLSLAALPYL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHH
GLLIYYWLGPQKVKRQRLRRGRSRSGMESYSSVCPPDADCTELAKIAQSTTGLAPSSATE
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCE
VHWLVDGAATYAAIIEAIRGARDHIHLEYYIFQPDHSGTAICAALMERARAGVKVRLLMD
EEEEECCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEHHH
AIGSSAMTRRALRTLREAGVETAWFHPSQLLKPFKRPWLNLRTHRKVIVIDGRVGFTGGI
HHCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCC
NVTDDENEQVRKQAYRDLHVRLQGHVVRSLQLVFLEDWLYATGQGRAAFHGQQLWPDDVP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
TRAQGTVDAQVLVSGPDSSWEAIHRLMVAAIHEAKHRVWLVTPYFVPGEAARMALTSAAL
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHH
GGLDVRLLVPRVSDSRLVTYAARSYFDELLEAGVRIYEYGPRMLHTKALLADDDVCIVGS
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCEEEEEC
ANFDSRSFRLNFELSMLFRDQAVAAEMAGLIGTDLQQAQEVQFARHRPLWRSRLPEAFAR
CCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHH
LLSPLL
HHHCCC
>Mature Secondary Structure
MLALAQGAWEWLATIPHLEALLLAAYVLYLLWIAGWIVLQKREPVATLSWVLSLAALPYL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHH
GLLIYYWLGPQKVKRQRLRRGRSRSGMESYSSVCPPDADCTELAKIAQSTTGLAPSSATE
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCE
VHWLVDGAATYAAIIEAIRGARDHIHLEYYIFQPDHSGTAICAALMERARAGVKVRLLMD
EEEEECCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEHHH
AIGSSAMTRRALRTLREAGVETAWFHPSQLLKPFKRPWLNLRTHRKVIVIDGRVGFTGGI
HHCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCC
NVTDDENEQVRKQAYRDLHVRLQGHVVRSLQLVFLEDWLYATGQGRAAFHGQQLWPDDVP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
TRAQGTVDAQVLVSGPDSSWEAIHRLMVAAIHEAKHRVWLVTPYFVPGEAARMALTSAAL
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHH
GGLDVRLLVPRVSDSRLVTYAARSYFDELLEAGVRIYEYGPRMLHTKALLADDDVCIVGS
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCEEEEEC
ANFDSRSFRLNFELSMLFRDQAVAAEMAGLIGTDLQQAQEVQFARHRPLWRSRLPEAFAR
CCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHH
LLSPLL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9443601 [H]