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The map label for this gene is opuE [H]

Identifier: 78045189

GI number: 78045189

Start: 100981

End: 102459

Strand: Direct

Name: opuE [H]

Synonym: CHY_0119

Alternate gene names: 78045189

Gene position: 100981-102459 (Clockwise)

Preceding gene: 78044391

Following gene: 78044039

Centisome position: 4.2

GC content: 46.32

Gene sequence:

>1479_bases
ATGGACAGGATGGTTAAGTTCTGGTTTTTGGGAATTTTTGTGTTTATTATGCTTGTTTCAGGATTTTTTGGGTTCAAGAA
AACGAAAACGGTGGATGATTTCATCTTAGGAGGCCGCAAGGTGGGTCCGTGGCTGTCGGCGTTTGCTTATGGCACGACCT
ACTTTTCTGCGGTTCTTTTTATTGGTTACGCCGGCAAAGTGGGCTTTAGCTTTGGGCTTCCGGCCCTCTGGATTGCTTTT
GGTAATGCGGTAATAGGAAGTTTTCTTGCCTGGAAGATATTAGCAAAACCTACCCGGTACATGACCCAGCAGTTAAATGC
CCGGACAATGCCGGAGTTTTTAGCAAAAAGGTATGATTTTCCCCTTTATAAGCCTCTGGCTGCTTTAATCATTTTCATTT
TTCTCGTACCCTACTCGGCGTCGGTATATATGGGCCTTAGTTACCTGTTTGAAGCGGTGTTTGGCTTGTCCTATCTTTTT
GCCCTTCTATTAATGGCCGGACTGACCGCAATCTATCTTTTACTGGGAGGTTATTTTGCCGTAGCTCTTACCGACTTTAT
CCAGGGAACAATTATGCTGTTAGGTGTTTTTCTCATGGTGGGGTACCTGGTAAATACGCCGGAAGTGGGGGGAATCAGCA
GCATTGCTTTTAAGCTTTCCCAGATTAATCCGGACTTTACTTCCCTCTGGGGTGGGCAGGGCTTCAGCTCTCTCATGTTT
TTAATGCTTTTAACCAGCTTCGGGAGCTGGGGTTTACCGCAAATGGTACAAAAATTTTACGCGATAAGGGATGAAAAGGC
AATAACACCGGCTACCTGGGTATCGACCCTCTTTGCTTTAGTTATTGCCGGGGGAGCGTATTTTACCGGAGCTTTAACGC
CTTTATTTTTTAAATCCCTGCCGGTAGATCCGGCAACAGGCAAACCTACCCCCGACCTTTTAGTTCCCCAGTTAATTGCC
CAGCACCTGCCGGAATGGGTGGCGGCGTTGATCTTACTCCTGGTTTTATCGGCATCGATGTCTACTTTGTCTTCCCTGGT
GCTGGTTTCAAGCTCGGCGGTAGCGGTAGACCTTTTGGGAGATACGGGCTTACTCAAAGATAAAAGTGGTCGCCAGATTT
TAGTGTTACGTATCTTAACGATAGTGTTTATTGCGTTTTCAGTGGTTATTGCCCTTTTAAAACCGGCTATTATTTTATCG
TTAATGGCCATATCCTGGGGAGCCGTGGCGGGAAGCTTTTTAGCTCCGTATTTCTGTGGCTTATTTTTCCGGCGGGTAAA
TGCGGTGGGCGCCCTGGCGGGAACCTTTACCGGCTTATTAACGGTGGTAACTTTAGCGGTAAAGCACGGCTTTGATGCCA
AAGTTCTGCCGGTAGCCGGGGTTTACGGCATTTTCTTACCCTTTGTGGTTGTACCTCTGGTAAGTTTATTAGTGCCGCTA
AAAGAAAGCAAGACGGTGAAACTTTTATTTGAAGAATAG

Upstream 100 bases:

>100_bases
GACAAAAAATTATCAATTTTGACAGAAAAGTTTATAGAAAATAATTTTAAAATAGTTTAAAATAGTAAGAAATTTAACTA
AATCTTTTAAGGACGGGAGG

Downstream 100 bases:

>100_bases
CGGGATGGAAGGGGAGGAGAACTTTGGGGTTTATCTGGAACAGAGAAAGAGAATGCATGTCCAGGAAGGACCTGGAAGAA
CTCCAGCTTGCGCGATTGAA

Product: osmoregulated proline transporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MDRMVKFWFLGIFVFIMLVSGFFGFKKTKTVDDFILGGRKVGPWLSAFAYGTTYFSAVLFIGYAGKVGFSFGLPALWIAF
GNAVIGSFLAWKILAKPTRYMTQQLNARTMPEFLAKRYDFPLYKPLAALIIFIFLVPYSASVYMGLSYLFEAVFGLSYLF
ALLLMAGLTAIYLLLGGYFAVALTDFIQGTIMLLGVFLMVGYLVNTPEVGGISSIAFKLSQINPDFTSLWGGQGFSSLMF
LMLLTSFGSWGLPQMVQKFYAIRDEKAITPATWVSTLFALVIAGGAYFTGALTPLFFKSLPVDPATGKPTPDLLVPQLIA
QHLPEWVAALILLLVLSASMSTLSSLVLVSSSAVAVDLLGDTGLLKDKSGRQILVLRILTIVFIAFSVVIALLKPAIILS
LMAISWGAVAGSFLAPYFCGLFFRRVNAVGALAGTFTGLLTVVTLAVKHGFDAKVLPVAGVYGIFLPFVVVPLVSLLVPL
KESKTVKLLFEE

Sequences:

>Translated_492_residues
MDRMVKFWFLGIFVFIMLVSGFFGFKKTKTVDDFILGGRKVGPWLSAFAYGTTYFSAVLFIGYAGKVGFSFGLPALWIAF
GNAVIGSFLAWKILAKPTRYMTQQLNARTMPEFLAKRYDFPLYKPLAALIIFIFLVPYSASVYMGLSYLFEAVFGLSYLF
ALLLMAGLTAIYLLLGGYFAVALTDFIQGTIMLLGVFLMVGYLVNTPEVGGISSIAFKLSQINPDFTSLWGGQGFSSLMF
LMLLTSFGSWGLPQMVQKFYAIRDEKAITPATWVSTLFALVIAGGAYFTGALTPLFFKSLPVDPATGKPTPDLLVPQLIA
QHLPEWVAALILLLVLSASMSTLSSLVLVSSSAVAVDLLGDTGLLKDKSGRQILVLRILTIVFIAFSVVIALLKPAIILS
LMAISWGAVAGSFLAPYFCGLFFRRVNAVGALAGTFTGLLTVVTLAVKHGFDAKVLPVAGVYGIFLPFVVVPLVSLLVPL
KESKTVKLLFEE
>Mature_492_residues
MDRMVKFWFLGIFVFIMLVSGFFGFKKTKTVDDFILGGRKVGPWLSAFAYGTTYFSAVLFIGYAGKVGFSFGLPALWIAF
GNAVIGSFLAWKILAKPTRYMTQQLNARTMPEFLAKRYDFPLYKPLAALIIFIFLVPYSASVYMGLSYLFEAVFGLSYLF
ALLLMAGLTAIYLLLGGYFAVALTDFIQGTIMLLGVFLMVGYLVNTPEVGGISSIAFKLSQINPDFTSLWGGQGFSSLMF
LMLLTSFGSWGLPQMVQKFYAIRDEKAITPATWVSTLFALVIAGGAYFTGALTPLFFKSLPVDPATGKPTPDLLVPQLIA
QHLPEWVAALILLLVLSASMSTLSSLVLVSSSAVAVDLLGDTGLLKDKSGRQILVLRILTIVFIAFSVVIALLKPAIILS
LMAISWGAVAGSFLAPYFCGLFFRRVNAVGALAGTFTGLLTVVTLAVKHGFDAKVLPVAGVYGIFLPFVVVPLVSLLVPL
KESKTVKLLFEE

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=450, Percent_Identity=27.3333333333333, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI14140236, Length=475, Percent_Identity=22.5263157894737, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI110835708, Length=493, Percent_Identity=24.3407707910751, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1787251, Length=441, Percent_Identity=29.2517006802721, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI87082237, Length=436, Percent_Identity=27.9816513761468, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI115533094, Length=423, Percent_Identity=23.6406619385343, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53396; Mature: 53396

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRMVKFWFLGIFVFIMLVSGFFGFKKTKTVDDFILGGRKVGPWLSAFAYGTTYFSAVLF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
IGYAGKVGFSFGLPALWIAFGNAVIGSFLAWKILAKPTRYMTQQLNARTMPEFLAKRYDF
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
PLYKPLAALIIFIFLVPYSASVYMGLSYLFEAVFGLSYLFALLLMAGLTAIYLLLGGYFA
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALTDFIQGTIMLLGVFLMVGYLVNTPEVGGISSIAFKLSQINPDFTSLWGGQGFSSLMF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCHHHHHH
LMLLTSFGSWGLPQMVQKFYAIRDEKAITPATWVSTLFALVIAGGAYFTGALTPLFFKSL
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
PVDPATGKPTPDLLVPQLIAQHLPEWVAALILLLVLSASMSTLSSLVLVSSSAVAVDLLG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DTGLLKDKSGRQILVLRILTIVFIAFSVVIALLKPAIILSLMAISWGAVAGSFLAPYFCG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFFRRVNAVGALAGTFTGLLTVVTLAVKHGFDAKVLPVAGVYGIFLPFVVVPLVSLLVPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
KESKTVKLLFEE
CCCCEEEEEECC
>Mature Secondary Structure
MDRMVKFWFLGIFVFIMLVSGFFGFKKTKTVDDFILGGRKVGPWLSAFAYGTTYFSAVLF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
IGYAGKVGFSFGLPALWIAFGNAVIGSFLAWKILAKPTRYMTQQLNARTMPEFLAKRYDF
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
PLYKPLAALIIFIFLVPYSASVYMGLSYLFEAVFGLSYLFALLLMAGLTAIYLLLGGYFA
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALTDFIQGTIMLLGVFLMVGYLVNTPEVGGISSIAFKLSQINPDFTSLWGGQGFSSLMF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCHHHHHH
LMLLTSFGSWGLPQMVQKFYAIRDEKAITPATWVSTLFALVIAGGAYFTGALTPLFFKSL
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
PVDPATGKPTPDLLVPQLIAQHLPEWVAALILLLVLSASMSTLSSLVLVSSSAVAVDLLG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DTGLLKDKSGRQILVLRILTIVFIAFSVVIALLKPAIILSLMAISWGAVAGSFLAPYFCG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFFRRVNAVGALAGTFTGLLTVVTLAVKHGFDAKVLPVAGVYGIFLPFVVVPLVSLLVPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
KESKTVKLLFEE
CCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]