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The map label for this gene is rlmH

Identifier: 78045158

GI number: 78045158

Start: 47337

End: 47816

Strand: Direct

Name: rlmH

Synonym: CHY_0058

Alternate gene names: 78045158

Gene position: 47337-47816 (Clockwise)

Preceding gene: 78044578

Following gene: 78043316

Centisome position: 1.97

GC content: 43.33

Gene sequence:

>480_bases
ATGGAAATTACAATTGTTGCGGTTGGCAAAATCAAAGAAAGGTATTTAAAAGAGGGGATAGCCGAGTATTTAAAACGGCT
ATCCCCTTATGCCCGTTTAGCGGTAATTGAGGTGGACGATGAAAACGCTCCGGAAAACCTATCGCCGGCGGAAGCCGAAA
AGGTAGTAAAAAAGGAAGGGGAGCGAATTTTAGCTAAAATTACCCCTTCTTCTTTTGTTATTGCCCTTGATCTTAAAGGA
AAAAATCTCTCATCTGAAGAGTTTGCCCATTTTATTTCGGAAAAGAATCTTTATGGGCAAAGTAAGCTTACTTTTATTAT
CGGCGGCTCTCTTGGCCTTTCCCGTGAGGTCCTGGAACGGGCGGATTTTAAGCTTTCTTTTGGCAGGATGACCTATCCCC
ACCAGCTGATGCGCTTAATCCTTTTAGAGCAGATCTACCGGGCCTTTAAAATAATCCGGGGCGAGCCTTATCATAAGTGA

Upstream 100 bases:

>100_bases
GACCTTTAAAGTTAAACTTGGCGAAATGCCGGCGGAGTAAGGGATGGTACCGGCAACCTGTGACTTTTGCGACCTTATAC
CCGTTTATCTGGTGGTGTAA

Downstream 100 bases:

>100_bases
TAAAGACAAAAATCTAGATAAAATAAAAAATATAACAAGATTTGGGTTTCTTCCAACTCCCCATGGTTTAACAGGGATAA
TTTTGAAAGGGGAGTTAACC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MEITIVAVGKIKERYLKEGIAEYLKRLSPYARLAVIEVDDENAPENLSPAEAEKVVKKEGERILAKITPSSFVIALDLKG
KNLSSEEFAHFISEKNLYGQSKLTFIIGGSLGLSREVLERADFKLSFGRMTYPHQLMRLILLEQIYRAFKIIRGEPYHK

Sequences:

>Translated_159_residues
MEITIVAVGKIKERYLKEGIAEYLKRLSPYARLAVIEVDDENAPENLSPAEAEKVVKKEGERILAKITPSSFVIALDLKG
KNLSSEEFAHFISEKNLYGQSKLTFIIGGSLGLSREVLERADFKLSFGRMTYPHQLMRLILLEQIYRAFKIIRGEPYHK
>Mature_159_residues
MEITIVAVGKIKERYLKEGIAEYLKRLSPYARLAVIEVDDENAPENLSPAEAEKVVKKEGERILAKITPSSFVIALDLKG
KNLSSEEFAHFISEKNLYGQSKLTFIIGGSLGLSREVLERADFKLSFGRMTYPHQLMRLILLEQIYRAFKIIRGEPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=30.188679245283, Blast_Score=84, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_CARHZ (Q3AG04)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_358930.1
- ProteinModelPortal:   Q3AG04
- SMR:   Q3AG04
- STRING:   Q3AG04
- GeneID:   3728284
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0058
- NMPDR:   fig|246194.3.peg.571
- TIGR:   CHY_0058
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- BioCyc:   CHYD246194:CHY_0058-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18114; Mature: 18114

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITIVAVGKIKERYLKEGIAEYLKRLSPYARLAVIEVDDENAPENLSPAEAEKVVKKEG
CEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEECCCCCCCCCCHHHHHHHHHHCC
ERILAKITPSSFVIALDLKGKNLSSEEFAHFISEKNLYGQSKLTFIIGGSLGLSREVLER
CEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHH
ADFKLSFGRMTYPHQLMRLILLEQIYRAFKIIRGEPYHK
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEITIVAVGKIKERYLKEGIAEYLKRLSPYARLAVIEVDDENAPENLSPAEAEKVVKKEG
CEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEECCCCCCCCCCHHHHHHHHHHCC
ERILAKITPSSFVIALDLKGKNLSSEEFAHFISEKNLYGQSKLTFIIGGSLGLSREVLER
CEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHH
ADFKLSFGRMTYPHQLMRLILLEQIYRAFKIIRGEPYHK
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA