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The map label for this gene is psrB [H]

Identifier: 78044751

GI number: 78044751

Start: 413699

End: 414295

Strand: Direct

Name: psrB [H]

Synonym: CHY_0477

Alternate gene names: 78044751

Gene position: 413699-414295 (Clockwise)

Preceding gene: 78044357

Following gene: 78042668

Centisome position: 17.23

GC content: 49.08

Gene sequence:

>597_bases
ATGGCACGCTACGGAATGGTAATTGACCTTCGAACCTGCGTCGGCTGTCAGGCCTGCAGTGCTGCCTGTGCCACCGAAAA
TCAGACGCCATACTGGTCCGATAAGTGGCGCACCCGGGTGCTGGATATCGAAAAAGGTGAATATCCCAATACCGGCCGGC
ATTTTGTACCAACAATTTGTATGCACTGCGAAGAACCGGCTTGTTTAACCATCTGTCCATCTCAGGCAACTTTCCGGGAT
GAAAACGGCAGCATTCTGGTAAATTATGATACCTGTCTTGGTTGTAAAGCCTGCATGGCGGCCTGCCCCTATGGGGCCAG
GTATGTTTACGATAAGAACGATGTTGCCAAAAACCGGGAAATTCACGGGGAACTTTCACAACATAAAGTGGTGCATATTG
ACAAGTGTACTTTTTGCTTTGACCGGGTAGAAAGAGGCCTGGAACCGGCTTGCGTGTCAACCTGTCCGGCTCATGCTAGG
ATTTTCGGGGACTTAGATGACCCGCATAGTGAAGTTGCCAGACTGGTGGCATCAGGAATTGCCAAGCCTTTAAGACCGGA
TTTAAATACCAAACCCAAGGTCTTTTACATTTACTAA

Upstream 100 bases:

>100_bases
TACCTTTAAATAATTTAGTTCCGTATAAAGTAGAACCTTTTGTGACCGGTTTTAAGTCTCAGGAATTTACCGTTAGGATA
ATAAAGTAAGGAGGAGAACA

Downstream 100 bases:

>100_bases
GGGGGGATGGCCGATGTCGGTGGAAAATAGTCTGCCTTTTGAACGTCAGGAAGCTTATGAATGGCCTTTAGCGGTATACT
TGTTGCTGGGAGGTATTGGC

Product: molybdopterin oxidoreductase, iron-sulfur cluster-binding subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Sulfur reductase chain B [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MARYGMVIDLRTCVGCQACSAACATENQTPYWSDKWRTRVLDIEKGEYPNTGRHFVPTICMHCEEPACLTICPSQATFRD
ENGSILVNYDTCLGCKACMAACPYGARYVYDKNDVAKNREIHGELSQHKVVHIDKCTFCFDRVERGLEPACVSTCPAHAR
IFGDLDDPHSEVARLVASGIAKPLRPDLNTKPKVFYIY

Sequences:

>Translated_198_residues
MARYGMVIDLRTCVGCQACSAACATENQTPYWSDKWRTRVLDIEKGEYPNTGRHFVPTICMHCEEPACLTICPSQATFRD
ENGSILVNYDTCLGCKACMAACPYGARYVYDKNDVAKNREIHGELSQHKVVHIDKCTFCFDRVERGLEPACVSTCPAHAR
IFGDLDDPHSEVARLVASGIAKPLRPDLNTKPKVFYIY
>Mature_197_residues
ARYGMVIDLRTCVGCQACSAACATENQTPYWSDKWRTRVLDIEKGEYPNTGRHFVPTICMHCEEPACLTICPSQATFRDE
NGSILVNYDTCLGCKACMAACPYGARYVYDKNDVAKNREIHGELSQHKVVHIDKCTFCFDRVERGLEPACVSTCPAHARI
FGDLDDPHSEVARLVASGIAKPLRPDLNTKPKVFYIY

Specific function: Component of the phosphorylative electron transport system with polysulfide as the terminal acceptor [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=197, Percent_Identity=36.5482233502538, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI226510944, Length=167, Percent_Identity=35.9281437125749, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1787122, Length=208, Percent_Identity=32.6923076923077, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787872, Length=209, Percent_Identity=30.622009569378, Blast_Score=99, Evalue=1e-22,
Organism=Escherichia coli, GI1787749, Length=199, Percent_Identity=32.6633165829146, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1789370, Length=198, Percent_Identity=29.7979797979798, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790326, Length=193, Percent_Identity=29.5336787564767, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1787740, Length=121, Percent_Identity=32.2314049586777, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1787478, Length=120, Percent_Identity=32.5, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789067, Length=169, Percent_Identity=33.7278106508876, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI2367245, Length=171, Percent_Identity=32.7485380116959, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI87082114, Length=206, Percent_Identity=31.5533980582524, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082180, Length=167, Percent_Identity=31.1377245508982, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI1789079, Length=106, Percent_Identity=33.9622641509434, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI87082179, Length=177, Percent_Identity=27.683615819209, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788811, Length=188, Percent_Identity=30.3191489361702, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 22164; Mature: 22032

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
10.1 %Cys+Met (Translated Protein)
8.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
9.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYGMVIDLRTCVGCQACSAACATENQTPYWSDKWRTRVLDIEKGEYPNTGRHFVPTIC
CCCCCEEEEHHHHHCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHH
MHCEEPACLTICPSQATFRDENGSILVNYDTCLGCKACMAACPYGARYVYDKNDVAKNRE
HCCCCCCEEEECCCCCEEECCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCCCCH
IHGELSQHKVVHIDKCTFCFDRVERGLEPACVSTCPAHARIFGDLDDPHSEVARLVASGI
HCCCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHCCCCHHEEECCCCCHHHHHHHHHHHHC
AKPLRPDLNTKPKVFYIY
CCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
ARYGMVIDLRTCVGCQACSAACATENQTPYWSDKWRTRVLDIEKGEYPNTGRHFVPTIC
CCCCEEEEHHHHHCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHH
MHCEEPACLTICPSQATFRDENGSILVNYDTCLGCKACMAACPYGARYVYDKNDVAKNRE
HCCCCCCEEEECCCCCEEECCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCCCCH
IHGELSQHKVVHIDKCTFCFDRVERGLEPACVSTCPAHARIFGDLDDPHSEVARLVASGI
HCCCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHCCCCHHEEECCCCCHHHHHHHHHHHHC
AKPLRPDLNTKPKVFYIY
CCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1597189; 14500908 [H]