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The map label for this gene is rne [H]

Identifier: 78044652

GI number: 78044652

Start: 313214

End: 314392

Strand: Direct

Name: rne [H]

Synonym: CHY_0364

Alternate gene names: 78044652

Gene position: 313214-314392 (Clockwise)

Preceding gene: 78042957

Following gene: 78044148

Centisome position: 13.04

GC content: 43.34

Gene sequence:

>1179_bases
ATGACTAAGACTTTAGTTTTTCACCGACAGCCGGAATACGATTTAGCGGCGGTAATGGTTGACGGAAGTCCTTTTTCGCT
TTTTGCGAAACGGCGAGACGAACAAACGCAGATTAACGATATTTATTTGGGGAAAGTAGAGAAAGTAGTTCCGGGGTTAC
AGGCGGCTTTTGTAAATTTAGGTTTGGCTAAAAACGGCTATTTACCTCTTGATGAGATAAACGTTAAGCCCGGGGATGTA
GTGTTGGTGAGGGTAGTTAAAAACCCCACAGCCCAAAAAGGCGCCAAGCTGTCTACGGTGATTTCCCTTCCGGGGCGAAA
CCTTGTTTATTTTCCCCAGAAAGAGATGAAAGGTATTTCCAGGAAACTTACGGAAGACAAAAGGGAGTATTATCATAGGG
TATTAGAAAACTTGAATCTTCCCGGCGGGTTTATTGTTAGAACTTTAGCCGGTGAGGAAGAAGACCTTTTGCAGGAAGCC
AAACGACTTTATGAAGAATGGCAGGGAATTTTAGAGCGAAGTAAAACTGCTCAAGCCCCGGCTTTACTTTATAAAGAAGA
TGACCTTTTTACCTGGTGTCTTAGGGAATTACTGGACAAAGACTTTACTGCCCTTTATGTGGATGATTTTGAACTTTTGG
AGATCGGGCAGAAGTTTCGCGAAAGGTTTCGCGTGTTGCTGCCGCCGATTTATAAAAGCTTTAATGTGATGGAAGAATTT
AACCTGATAAGGACAATGGAAAAACTTACCAGGGAGCGGGTCTGGCTGAAAAACGGCGGTATTTTAGTCATCGAAAAAAC
CGAGGCTTTAACCGCCATTGATGTAAACTCTGGAAAATTCACCGGAAAAAAAGATTTAAAAGAAACCGCTTTTAAAATAA
ATTTAGAAGCGGCCCGGGAAATTGCCAAGCAGATTAGGTTAAGAAATATCGGTGGAATTATCGTGGTAGATTTTATTTCC
CTGTTGGAGGAGGAACGGTGGGAAGAGATTTTAAGGGTTATGGAAGAAGAGTTTTTGCGGGATAAAGCCAAAGTAAAGAT
TACCGGGAAAACTCCTTCGGGGCTGGTGGAAATTGCCCGGCAGAAAATAGGGCTTTCCATCGGCGAGCTTTTTACCCACG
AGTGCAGTGTCTGCCAGGGCACCGGGAGAATAACAGATATTACTGTCAAGCGTCTCTGA

Upstream 100 bases:

>100_bases
CCTTGCGTCCGGAAGAGGTAGCTAAAATTTTTTTGGGTTATTTGCCCGAGGATATTGAATATATTCGGGAAGAAATTATT
TTAAGTTAAAGGGAGCTATC

Downstream 100 bases:

>100_bases
CTTTATCACCTTTATGTGTCTCGACTTTGACCCGTTGAGCGGATACAAGAGTGACAATATCACAGATTAATAACAATCGC
CAACCTTATTGATTGACTAT

Product: ribonuclease

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MTKTLVFHRQPEYDLAAVMVDGSPFSLFAKRRDEQTQINDIYLGKVEKVVPGLQAAFVNLGLAKNGYLPLDEINVKPGDV
VLVRVVKNPTAQKGAKLSTVISLPGRNLVYFPQKEMKGISRKLTEDKREYYHRVLENLNLPGGFIVRTLAGEEEDLLQEA
KRLYEEWQGILERSKTAQAPALLYKEDDLFTWCLRELLDKDFTALYVDDFELLEIGQKFRERFRVLLPPIYKSFNVMEEF
NLIRTMEKLTRERVWLKNGGILVIEKTEALTAIDVNSGKFTGKKDLKETAFKINLEAAREIAKQIRLRNIGGIIVVDFIS
LLEEERWEEILRVMEEEFLRDKAKVKITGKTPSGLVEIARQKIGLSIGELFTHECSVCQGTGRITDITVKRL

Sequences:

>Translated_392_residues
MTKTLVFHRQPEYDLAAVMVDGSPFSLFAKRRDEQTQINDIYLGKVEKVVPGLQAAFVNLGLAKNGYLPLDEINVKPGDV
VLVRVVKNPTAQKGAKLSTVISLPGRNLVYFPQKEMKGISRKLTEDKREYYHRVLENLNLPGGFIVRTLAGEEEDLLQEA
KRLYEEWQGILERSKTAQAPALLYKEDDLFTWCLRELLDKDFTALYVDDFELLEIGQKFRERFRVLLPPIYKSFNVMEEF
NLIRTMEKLTRERVWLKNGGILVIEKTEALTAIDVNSGKFTGKKDLKETAFKINLEAAREIAKQIRLRNIGGIIVVDFIS
LLEEERWEEILRVMEEEFLRDKAKVKITGKTPSGLVEIARQKIGLSIGELFTHECSVCQGTGRITDITVKRL
>Mature_391_residues
TKTLVFHRQPEYDLAAVMVDGSPFSLFAKRRDEQTQINDIYLGKVEKVVPGLQAAFVNLGLAKNGYLPLDEINVKPGDVV
LVRVVKNPTAQKGAKLSTVISLPGRNLVYFPQKEMKGISRKLTEDKREYYHRVLENLNLPGGFIVRTLAGEEEDLLQEAK
RLYEEWQGILERSKTAQAPALLYKEDDLFTWCLRELLDKDFTALYVDDFELLEIGQKFRERFRVLLPPIYKSFNVMEEFN
LIRTMEKLTRERVWLKNGGILVIEKTEALTAIDVNSGKFTGKKDLKETAFKINLEAAREIAKQIRLRNIGGIIVVDFISL
LEEERWEEILRVMEEEFLRDKAKVKITGKTPSGLVEIARQKIGLSIGELFTHECSVCQGTGRITDITVKRL

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=419, Percent_Identity=33.1742243436754, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI87082235, Length=424, Percent_Identity=33.7264150943396, Blast_Score=201, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 44895; Mature: 44764

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLVFHRQPEYDLAAVMVDGSPFSLFAKRRDEQTQINDIYLGKVEKVVPGLQAAFVNL
CCCEEEEECCCCCCEEEEEECCCCHHHHHHCCCCCHHHHHEEHHHHHHHHCCHHHHHHHC
GLAKNGYLPLDEINVKPGDVVLVRVVKNPTAQKGAKLSTVISLPGRNLVYFPQKEMKGIS
CCCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCEEECCHHHHHHHH
RKLTEDKREYYHRVLENLNLPGGFIVRTLAGEEEDLLQEAKRLYEEWQGILERSKTAQAP
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ALLYKEDDLFTWCLRELLDKDFTALYVDDFELLEIGQKFRERFRVLLPPIYKSFNVMEEF
EEEEECCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLIRTMEKLTRERVWLKNGGILVIEKTEALTAIDVNSGKFTGKKDLKETAFKINLEAARE
HHHHHHHHHHHHHEEEECCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHEEEHHHHHH
IAKQIRLRNIGGIIVVDFISLLEEERWEEILRVMEEEFLRDKAKVKITGKTPSGLVEIAR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
QKIGLSIGELFTHECSVCQGTGRITDITVKRL
HHHCCHHHHHHHHHHHHHCCCCCEEEEEEECC
>Mature Secondary Structure 
TKTLVFHRQPEYDLAAVMVDGSPFSLFAKRRDEQTQINDIYLGKVEKVVPGLQAAFVNL
CCEEEEECCCCCCEEEEEECCCCHHHHHHCCCCCHHHHHEEHHHHHHHHCCHHHHHHHC
GLAKNGYLPLDEINVKPGDVVLVRVVKNPTAQKGAKLSTVISLPGRNLVYFPQKEMKGIS
CCCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCEEECCHHHHHHHH
RKLTEDKREYYHRVLENLNLPGGFIVRTLAGEEEDLLQEAKRLYEEWQGILERSKTAQAP
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ALLYKEDDLFTWCLRELLDKDFTALYVDDFELLEIGQKFRERFRVLLPPIYKSFNVMEEF
EEEEECCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLIRTMEKLTRERVWLKNGGILVIEKTEALTAIDVNSGKFTGKKDLKETAFKINLEAARE
HHHHHHHHHHHHHEEEECCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHEEEHHHHHH
IAKQIRLRNIGGIIVVDFISLLEEERWEEILRVMEEEFLRDKAKVKITGKTPSGLVEIAR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
QKIGLSIGELFTHECSVCQGTGRITDITVKRL
HHHCCHHHHHHHHHHHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]