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The map label for this gene is dnaG [H]

Identifier: 78044503

GI number: 78044503

Start: 397519

End: 399246

Strand: Direct

Name: dnaG [H]

Synonym: CHY_0454

Alternate gene names: 78044503

Gene position: 397519-399246 (Clockwise)

Preceding gene: 78045165

Following gene: 78044024

Centisome position: 16.55

GC content: 37.56

Gene sequence:

>1728_bases
GTGTCCTTTCAAGAGGTTGTAGAAAAAATTAGGAACCGGGTCGATTTAGTTGATTTAGTTAGCGAATATACCAGGCTGAA
AAAGTCGGGAAGGAACTATGTAGGATTATGTCCCTTTCATAATGAAAAAACCCCTTCCTTTTCGGTTTCACCGGAAAAAA
ACCTGTATTACTGCTTTGGTTGCCAAAAAGGCGGCGATATTTTTAGTTTTATCATGGAAAAAGAAAGGTTGAATTTTAAA
GAAGCCGTCTTTTATTTGGCGAAAAAATATAACATTGAACTTCCGGAAACGGGAAAAGCGTATAATAAAAACGAACATCT
GTACCGAATGTTACTTTTAGCGGCAAAATTTTACCATGAAATTTTAAAAACCGAAGCGGCCGGGAAAGCCCGGGATTACC
TGCAAAGCAGGGGTATTACCGAGGAAACGGTTAAAGATTTTATGTTAGGCTTTGCTCCATCCGCCAAAGCTTTGCCGGCT
TTTTTAAGCAGTAAAGGTTATAATCCGGGGGAACTGGTTAAAATAGGAATTGTGACAAAAAGTTATAGCGGAAACTTACA
TGACCGTTTTGCTGGAAGGCTTATCTTTCCCATATTCGACCGGGAGGGCCGGGTGGTAGGTTTTGGGGGAAGAGCCCTGG
GCGAGGAAAAGCCCAAGTATTTTAATTCACCCCAATCGGTGATTTTTGACAAGGGTAAGCTTTTTTACGGTTTTAAACAA
GCTCGAACCACAATCAGGGAAAAAAGACAGGCCATTTTAGTTGAAGGATATTTTGACGTGATTTCTTTACATCAGGCAGG
AGTTAAAAATGCTATTGCGTGTTTGGGTACGGCATTTACCGAAGAACATTTAAATTTTTTAAAAAACTACGGTTATATTG
AGGAAATAATCTTAGCTTACGATAGCGACCAGGCGGGAGAAAAAGCAGCCTATAGAGCACTGGAAATGCTGGTTCGGGCC
AGTGCTGATTTAAGCATTAAAATCATTCGTCTTGGAGAATTTAAAGATCCCGATGAGCTTATTAAAAATCGGGGAGCAAC
TGCTTTTTTAGAAGAAATAGAAAAAGCCAAAACTCCTGCGGAATTTTTATTAGAATTAGAGCTTAAAGAAAAGAGCAGTT
TAAATCTTAACGAACAAAGGGCGCTGTTAAAAAAGCTTATCCCTTATCTGGTTTTAGAATACGAAAAAACTCCGGCTTTG
TTTGCCGAAAGAATAGGCGGCATTGCCAGCAAGCTTAAAATGAGCGAAGGGGCATTAGTGGACGAAATTCGCCGAGAGAT
TTATAAAAATGAAGAAAAATCTTTAAATAATTCAAGTTTTGGAGAAAATATAACTAATGAACACAAAAATTATGGCCTTT
TAAAAGTTGAAAGTGAGTTATTGGGTTTGTTATTAGAATATCCTGAGTATCAAAAAGAATTTTTAAATGACTTTTTTTTC
TATGAGTTTCAGGGAAAAAACCAGGAAGTCTTTAATTACCTAAAGGAAAATAAGTTCCCTGACCGGGAAGATCCATTATA
TAGTTACATGACCTATTTAAGGATTAACTGCCCGGAAGGGGATCCGAAGACCCTTTATGAAGGGTATGTTCGCTATCTTG
AAGAACATTATAAAAAGCAGCAAGTTCAAAGGTTAACATTGGAAATATCTTTAGCGGAAAAAGAAGGAAACTGGGAAAAA
CTCCAGTTATTATTAAACAAACTGCAGGAACTTACCCAAAAAAACTAA

Upstream 100 bases:

>100_bases
TGTTTATACCTCATCCTTTGACCTAAAAACTTATTTGTCGAAGCAGCAGGAAAAAGCGAAAAAAAAGAGAATAACTATTA
TTAAGAGAGCGGTGAAAACC

Downstream 100 bases:

>100_bases
CGAAGAAAGGGGGGAATGCGGCATGAAGGAAATAATGCAAAATGAAAAGCTTATGGAAATTGTTGAAAAAGGTAAAAAGC
GCGGCGTTTTAACCTATAAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MSFQEVVEKIRNRVDLVDLVSEYTRLKKSGRNYVGLCPFHNEKTPSFSVSPEKNLYYCFGCQKGGDIFSFIMEKERLNFK
EAVFYLAKKYNIELPETGKAYNKNEHLYRMLLLAAKFYHEILKTEAAGKARDYLQSRGITEETVKDFMLGFAPSAKALPA
FLSSKGYNPGELVKIGIVTKSYSGNLHDRFAGRLIFPIFDREGRVVGFGGRALGEEKPKYFNSPQSVIFDKGKLFYGFKQ
ARTTIREKRQAILVEGYFDVISLHQAGVKNAIACLGTAFTEEHLNFLKNYGYIEEIILAYDSDQAGEKAAYRALEMLVRA
SADLSIKIIRLGEFKDPDELIKNRGATAFLEEIEKAKTPAEFLLELELKEKSSLNLNEQRALLKKLIPYLVLEYEKTPAL
FAERIGGIASKLKMSEGALVDEIRREIYKNEEKSLNNSSFGENITNEHKNYGLLKVESELLGLLLEYPEYQKEFLNDFFF
YEFQGKNQEVFNYLKENKFPDREDPLYSYMTYLRINCPEGDPKTLYEGYVRYLEEHYKKQQVQRLTLEISLAEKEGNWEK
LQLLLNKLQELTQKN

Sequences:

>Translated_575_residues
MSFQEVVEKIRNRVDLVDLVSEYTRLKKSGRNYVGLCPFHNEKTPSFSVSPEKNLYYCFGCQKGGDIFSFIMEKERLNFK
EAVFYLAKKYNIELPETGKAYNKNEHLYRMLLLAAKFYHEILKTEAAGKARDYLQSRGITEETVKDFMLGFAPSAKALPA
FLSSKGYNPGELVKIGIVTKSYSGNLHDRFAGRLIFPIFDREGRVVGFGGRALGEEKPKYFNSPQSVIFDKGKLFYGFKQ
ARTTIREKRQAILVEGYFDVISLHQAGVKNAIACLGTAFTEEHLNFLKNYGYIEEIILAYDSDQAGEKAAYRALEMLVRA
SADLSIKIIRLGEFKDPDELIKNRGATAFLEEIEKAKTPAEFLLELELKEKSSLNLNEQRALLKKLIPYLVLEYEKTPAL
FAERIGGIASKLKMSEGALVDEIRREIYKNEEKSLNNSSFGENITNEHKNYGLLKVESELLGLLLEYPEYQKEFLNDFFF
YEFQGKNQEVFNYLKENKFPDREDPLYSYMTYLRINCPEGDPKTLYEGYVRYLEEHYKKQQVQRLTLEISLAEKEGNWEK
LQLLLNKLQELTQKN
>Mature_574_residues
SFQEVVEKIRNRVDLVDLVSEYTRLKKSGRNYVGLCPFHNEKTPSFSVSPEKNLYYCFGCQKGGDIFSFIMEKERLNFKE
AVFYLAKKYNIELPETGKAYNKNEHLYRMLLLAAKFYHEILKTEAAGKARDYLQSRGITEETVKDFMLGFAPSAKALPAF
LSSKGYNPGELVKIGIVTKSYSGNLHDRFAGRLIFPIFDREGRVVGFGGRALGEEKPKYFNSPQSVIFDKGKLFYGFKQA
RTTIREKRQAILVEGYFDVISLHQAGVKNAIACLGTAFTEEHLNFLKNYGYIEEIILAYDSDQAGEKAAYRALEMLVRAS
ADLSIKIIRLGEFKDPDELIKNRGATAFLEEIEKAKTPAEFLLELELKEKSSLNLNEQRALLKKLIPYLVLEYEKTPALF
AERIGGIASKLKMSEGALVDEIRREIYKNEEKSLNNSSFGENITNEHKNYGLLKVESELLGLLLEYPEYQKEFLNDFFFY
EFQGKNQEVFNYLKENKFPDREDPLYSYMTYLRINCPEGDPKTLYEGYVRYLEEHYKKQQVQRLTLEISLAEKEGNWEKL
QLLLNKLQELTQKN

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=389, Percent_Identity=38.560411311054, Blast_Score=274, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 66420; Mature: 66289

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFQEVVEKIRNRVDLVDLVSEYTRLKKSGRNYVGLCPFHNEKTPSFSVSPEKNLYYCFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEE
CQKGGDIFSFIMEKERLNFKEAVFYLAKKYNIELPETGKAYNKNEHLYRMLLLAAKFYHE
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ILKTEAAGKARDYLQSRGITEETVKDFMLGFAPSAKALPAFLSSKGYNPGELVKIGIVTK
HHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEEEE
SYSGNLHDRFAGRLIFPIFDREGRVVGFGGRALGEEKPKYFNSPQSVIFDKGKLFYGFKQ
CCCCCCHHHHCCEEEEEEECCCCCEEEECCHHCCCCCCCCCCCCHHHEEECCCEEEHHHH
ARTTIREKRQAILVEGYFDVISLHQAGVKNAIACLGTAFTEEHLNFLKNYGYIEEIILAY
HHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
DSDQAGEKAAYRALEMLVRASADLSIKIIRLGEFKDPDELIKNRGATAFLEEIEKAKTPA
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHCCCH
EFLLELELKEKSSLNLNEQRALLKKLIPYLVLEYEKTPALFAERIGGIASKLKMSEGALV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHH
DEIRREIYKNEEKSLNNSSFGENITNEHKNYGLLKVESELLGLLLEYPEYQKEFLNDFFF
HHHHHHHHCCHHHCCCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHHHHEE
YEFQGKNQEVFNYLKENKFPDREDPLYSYMTYLRINCPEGDPKTLYEGYVRYLEEHYKKQ
EEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHH
QVQRLTLEISLAEKEGNWEKLQLLLNKLQELTQKN
HHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFQEVVEKIRNRVDLVDLVSEYTRLKKSGRNYVGLCPFHNEKTPSFSVSPEKNLYYCFG
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEE
CQKGGDIFSFIMEKERLNFKEAVFYLAKKYNIELPETGKAYNKNEHLYRMLLLAAKFYHE
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ILKTEAAGKARDYLQSRGITEETVKDFMLGFAPSAKALPAFLSSKGYNPGELVKIGIVTK
HHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEEEE
SYSGNLHDRFAGRLIFPIFDREGRVVGFGGRALGEEKPKYFNSPQSVIFDKGKLFYGFKQ
CCCCCCHHHHCCEEEEEEECCCCCEEEECCHHCCCCCCCCCCCCHHHEEECCCEEEHHHH
ARTTIREKRQAILVEGYFDVISLHQAGVKNAIACLGTAFTEEHLNFLKNYGYIEEIILAY
HHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
DSDQAGEKAAYRALEMLVRASADLSIKIIRLGEFKDPDELIKNRGATAFLEEIEKAKTPA
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHCCCH
EFLLELELKEKSSLNLNEQRALLKKLIPYLVLEYEKTPALFAERIGGIASKLKMSEGALV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHH
DEIRREIYKNEEKSLNNSSFGENITNEHKNYGLLKVESELLGLLLEYPEYQKEFLNDFFF
HHHHHHHHCCHHHCCCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHHHHEE
YEFQGKNQEVFNYLKENKFPDREDPLYSYMTYLRINCPEGDPKTLYEGYVRYLEEHYKKQ
EEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHH
QVQRLTLEISLAEKEGNWEKLQLLLNKLQELTQKN
HHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]