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The map label for this gene is ligT [C]

Identifier: 78044190

GI number: 78044190

Start: 395202

End: 395735

Strand: Direct

Name: ligT [C]

Synonym: CHY_0451

Alternate gene names: 78044190

Gene position: 395202-395735 (Clockwise)

Preceding gene: 78044782

Following gene: 78042953

Centisome position: 16.46

GC content: 32.77

Gene sequence:

>534_bases
ATGCGTTTATTTTTTGCAGTGGATTTTCCCGAGGAATTAAAAGAAAAAATTCATCAGGATATTACACCTTTATTTAAATA
CAACGGGGTTAAGTGGGTAGCAAAAGAGAACTACCACTTAACTTTAGCTTTTTTGGGGGAAAAAGAGGAAAAGGAGCTTT
TGCAAATTGACCGGGCGGCAAAGAGCGTACCTCAAGTCAAAGAGTCATTAATCAAAGTATTAGATTTTGGGGCTTTTCCC
AATTTAAAAAGGCCTAAAGTTTTATTTTTAAAAATCGAAAGTCCCGGGCTTTTAACTTTAGCCGAGAATTTACGTCAAAA
TTTAAAAGAAAATAATATTTCTTTTGACGAAAAGCCGTTTGTGCCCCATTTAACCATAGGCAGGGTAAAAGGGGAAATAA
AAAATTTAGCTTTACCAAAGTTTAACCCCAAAGAATTTTCTTTTAAAAACTTTTACTTAATAAAAAGCATTTTAACCCCT
GAAGGACCGATTTACGAAAAAATAAAAAGCTACGAAATATTACAAAATGCTTAA

Upstream 100 bases:

>100_bases
CATTGGCAGGATTTTTCTTTTCTTTAGGGAGAAAAAAAGGATTTTTTACTTTTGCGGCAAATAATAAATATACTTATTTT
ATTGACAGTGGGGGATAAAG

Downstream 100 bases:

>100_bases
GAATTTGTTAAAATTAAATTTTATGAAGGAAAAAGCCAATTAAAAAGCGTATTAGGAAGGTATAAAGGAGGCATATAAAT
GAAGGTTGTTGTTTTAAATG

Product: putative 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MRLFFAVDFPEELKEKIHQDITPLFKYNGVKWVAKENYHLTLAFLGEKEEKELLQIDRAAKSVPQVKESLIKVLDFGAFP
NLKRPKVLFLKIESPGLLTLAENLRQNLKENNISFDEKPFVPHLTIGRVKGEIKNLALPKFNPKEFSFKNFYLIKSILTP
EGPIYEKIKSYEILQNA

Sequences:

>Translated_177_residues
MRLFFAVDFPEELKEKIHQDITPLFKYNGVKWVAKENYHLTLAFLGEKEEKELLQIDRAAKSVPQVKESLIKVLDFGAFP
NLKRPKVLFLKIESPGLLTLAENLRQNLKENNISFDEKPFVPHLTIGRVKGEIKNLALPKFNPKEFSFKNFYLIKSILTP
EGPIYEKIKSYEILQNA
>Mature_177_residues
MRLFFAVDFPEELKEKIHQDITPLFKYNGVKWVAKENYHLTLAFLGEKEEKELLQIDRAAKSVPQVKESLIKVLDFGAFP
NLKRPKVLFLKIESPGLLTLAENLRQNLKENNISFDEKPFVPHLTIGRVKGEIKNLALPKFNPKEFSFKNFYLIKSILTP
EGPIYEKIKSYEILQNA

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

Organism=Escherichia coli, GI87081692, Length=176, Percent_Identity=30.6818181818182, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20546; Mature: 20546

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFFAVDFPEELKEKIHQDITPLFKYNGVKWVAKENYHLTLAFLGEKEEKELLQIDRAA
CEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHHH
KSVPQVKESLIKVLDFGAFPNLKRPKVLFLKIESPGLLTLAENLRQNLKENNISFDEKPF
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCC
VPHLTIGRVKGEIKNLALPKFNPKEFSFKNFYLIKSILTPEGPIYEKIKSYEILQNA
CCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLFFAVDFPEELKEKIHQDITPLFKYNGVKWVAKENYHLTLAFLGEKEEKELLQIDRAA
CEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHHH
KSVPQVKESLIKVLDFGAFPNLKRPKVLFLKIESPGLLTLAENLRQNLKENNISFDEKPF
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCC
VPHLTIGRVKGEIKNLALPKFNPKEFSFKNFYLIKSILTPEGPIYEKIKSYEILQNA
CCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]