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The map label for this gene is lutB [H]

Identifier: 78044095

GI number: 78044095

Start: 378076

End: 380196

Strand: Direct

Name: lutB [H]

Synonym: CHY_0436

Alternate gene names: 78044095

Gene position: 378076-380196 (Clockwise)

Preceding gene: 78044955

Following gene: 78044245

Centisome position: 15.74

GC content: 45.87

Gene sequence:

>2121_bases
ATGGCGAATAAAGAGAAAATTAAAAAAGCCCTTGGTAATCCTACCTTACGGCGTGCTCTAACCAATTTTGGAAATAATTA
TGTTTTGGCTCGGGAAAAAGTTTTTGCCGGCCTTGATTTTGAGGAACTACGCCGGGAGATCAAGGCGGCCAGAACCGAGG
TTAGAAAAAAGCTTGATTTTTACGTGGAACAATTCAGGATAAATGCCGAAAAAAATGGTGCCAGGGTAATAATTGTCCAC
TCGGCCGCTGAAGCAAAAAAAGCAATAGTTGATATTTTAAAGGCCAAAAACGCCAAAACAGTGGTAAAATCAAAATCCAT
GGCATCTGAAGAAATTCACCTGAATGAAGAGCTTGTAAAACACGGCATTGAAGTAGTGGAAACCGACCTCGGGGAGTGGA
TTTTACAGCTTGACGGGCAAAAGCCCTCCCACATGGTAATGCCGGCAATCCACCTTACCCGTGAAGAAGTGGCTGCTATT
TTTTCCAGGGCACTGGACCGGGAAGTAGCTCCGGATATTGCCCAAATGGTTAAGCTTGCCCGACAGGAATTAAGGGATAA
GTTTTTAAAAGCCGATGTCGGGATTACCGGTGGAAATATTGCGGTGGCAGAAACCGGTTCGATTTTTCTCTTTACCAATG
AAGGGAATGCCCGGTTAACAACTACCTTGCCCAGAGTACGGATTAGCTTGGTGGGGGTTGAAAAAATTGTACCAACGTTA
AAGGATGCCATCCCAATCATGCGGGCTCTTCCCCGAAACGCTACTGCTCAGAAAATTACCAGCTACGTGACCGTGATGAC
TGCGGGAGCCGGTCAGGAACATTATGTAATTTTAATGGATAATGGACGCATTGAGCTAAAAGACGACCCGGTGTTCGCAG
AACTTTTCCAGTGTATCCGCTGCGCCGGTTGTTTAAATGTGTGTCCCGTCTACCAGCAAGTAGGGGGCCATGTCTTTGGG
CATGTTTATACCGGACCTATCGGTACCCTTTTGACCGCTTTCTTTCACGGATTGGATGCAGCCAAAGACCCTCAGGCCCT
CTGCGGCAGTTGCCTTAGATGTGCTACCGTTTGCCCTGCGGGCATAAATTTACCGGAATTGTTGTTAAAATTACGTCAAA
GGGTTGTTGAAAGTTATGGACAACCGTTTGTCCAAAAATTTATCTTTGAACGAATTCTGGCCAATCCCAAGCTTTTCAAC
TCTCTTTTAGACCTTGGTCGAAAATTTCAGGGGTTAATCGCGGAAAACGGCGAGATTAAATCTTTACCCTTTGGTTTGGA
TAATCTTACTTCTTTCAGGGTGTTTCCAGCTCTTGCCAAGGAGCGACTTTATAAAAAATATCGGGTGCGTCCCAAGAAAA
AAGGTACCAGGGGCAAAGTGCTGTTTTACAGCGGTTGCTTAATTGAACACATGTATCCCGATATTGGTGAAGATGTTTTC
TTGGTTTTAGAGAAAAACGGTTATGAAGTGGTTTTACCGGAAGATCTGGGATGTTGCGGGGCTCCAGCATATTACTCCGG
AGACTTACAGGCTGCCGTGAGGATGGCTAAAAACAACATTGAGGCGTTTTTAAAGCACGAGTATGATTATATAGTCACTG
CCTGTCCTACCTGCAGCGAAACCCTAATGCATTATGAAAAATATTTAGAGGATGACGAAGAGTACCGGGAGAAAGCCAGG
GAAGTATCGAGGAAGGTAATCGATTTTACCGATTTTGCTTATAAGTATTTGGCGGTGGGTGAAGGAAAACTGGCGGGGAG
CTATACCCTTCATGACTCCTGTCATGCCCGCCGGGGCCTGGGTCAGGTAAAAGAGCCCCGGGAGCTAATTGTTAAAACCG
GGGCAAACCTGGTGGAGATGGCCAATTCCGACCAGTGCTGCGGTTTTGGCGGTTCCTTCTCCATAAAGTATCCGGAAATC
TCCGAAAAAGTGTTTCAGAATAAATACCAAAGCATCATGGAAACCGGTGCAGAAAAAGTAGCGGTTACCTGTCCGGGGTG
TTTAATGCAAATTGCCGGCGGCCTGAAAAAACAGGGAGCTCCGGTAAAGGTAGAGCACTTAGCTACCATCCTGGCTAAAA
CCTTTAGGGACGGTTCTGAAAACCACCACTTTCTACGATAG

Upstream 100 bases:

>100_bases
TCACCGGACCTTCCAGAACGGCCGATATTGAAAGGGTTTTGACCATCGGGGTTCATGGACCGGGCCGACTTATAGTATTT
ATAAAAGAGGGTGAGGAATA

Downstream 100 bases:

>100_bases
ATAGAAAGTGGTGGCTGGTAGAACCGTCCCTTCCGGTTACTTTTTTGCTTCTTCCCGGAGAGCCCGCCGTAAAACTTTAC
CTACGGTAGTTTTGGGGAGT

Product: iron-sulfur cluster binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 706; Mature: 705

Protein sequence:

>706_residues
MANKEKIKKALGNPTLRRALTNFGNNYVLAREKVFAGLDFEELRREIKAARTEVRKKLDFYVEQFRINAEKNGARVIIVH
SAAEAKKAIVDILKAKNAKTVVKSKSMASEEIHLNEELVKHGIEVVETDLGEWILQLDGQKPSHMVMPAIHLTREEVAAI
FSRALDREVAPDIAQMVKLARQELRDKFLKADVGITGGNIAVAETGSIFLFTNEGNARLTTTLPRVRISLVGVEKIVPTL
KDAIPIMRALPRNATAQKITSYVTVMTAGAGQEHYVILMDNGRIELKDDPVFAELFQCIRCAGCLNVCPVYQQVGGHVFG
HVYTGPIGTLLTAFFHGLDAAKDPQALCGSCLRCATVCPAGINLPELLLKLRQRVVESYGQPFVQKFIFERILANPKLFN
SLLDLGRKFQGLIAENGEIKSLPFGLDNLTSFRVFPALAKERLYKKYRVRPKKKGTRGKVLFYSGCLIEHMYPDIGEDVF
LVLEKNGYEVVLPEDLGCCGAPAYYSGDLQAAVRMAKNNIEAFLKHEYDYIVTACPTCSETLMHYEKYLEDDEEYREKAR
EVSRKVIDFTDFAYKYLAVGEGKLAGSYTLHDSCHARRGLGQVKEPRELIVKTGANLVEMANSDQCCGFGGSFSIKYPEI
SEKVFQNKYQSIMETGAEKVAVTCPGCLMQIAGGLKKQGAPVKVEHLATILAKTFRDGSENHHFLR

Sequences:

>Translated_706_residues
MANKEKIKKALGNPTLRRALTNFGNNYVLAREKVFAGLDFEELRREIKAARTEVRKKLDFYVEQFRINAEKNGARVIIVH
SAAEAKKAIVDILKAKNAKTVVKSKSMASEEIHLNEELVKHGIEVVETDLGEWILQLDGQKPSHMVMPAIHLTREEVAAI
FSRALDREVAPDIAQMVKLARQELRDKFLKADVGITGGNIAVAETGSIFLFTNEGNARLTTTLPRVRISLVGVEKIVPTL
KDAIPIMRALPRNATAQKITSYVTVMTAGAGQEHYVILMDNGRIELKDDPVFAELFQCIRCAGCLNVCPVYQQVGGHVFG
HVYTGPIGTLLTAFFHGLDAAKDPQALCGSCLRCATVCPAGINLPELLLKLRQRVVESYGQPFVQKFIFERILANPKLFN
SLLDLGRKFQGLIAENGEIKSLPFGLDNLTSFRVFPALAKERLYKKYRVRPKKKGTRGKVLFYSGCLIEHMYPDIGEDVF
LVLEKNGYEVVLPEDLGCCGAPAYYSGDLQAAVRMAKNNIEAFLKHEYDYIVTACPTCSETLMHYEKYLEDDEEYREKAR
EVSRKVIDFTDFAYKYLAVGEGKLAGSYTLHDSCHARRGLGQVKEPRELIVKTGANLVEMANSDQCCGFGGSFSIKYPEI
SEKVFQNKYQSIMETGAEKVAVTCPGCLMQIAGGLKKQGAPVKVEHLATILAKTFRDGSENHHFLR
>Mature_705_residues
ANKEKIKKALGNPTLRRALTNFGNNYVLAREKVFAGLDFEELRREIKAARTEVRKKLDFYVEQFRINAEKNGARVIIVHS
AAEAKKAIVDILKAKNAKTVVKSKSMASEEIHLNEELVKHGIEVVETDLGEWILQLDGQKPSHMVMPAIHLTREEVAAIF
SRALDREVAPDIAQMVKLARQELRDKFLKADVGITGGNIAVAETGSIFLFTNEGNARLTTTLPRVRISLVGVEKIVPTLK
DAIPIMRALPRNATAQKITSYVTVMTAGAGQEHYVILMDNGRIELKDDPVFAELFQCIRCAGCLNVCPVYQQVGGHVFGH
VYTGPIGTLLTAFFHGLDAAKDPQALCGSCLRCATVCPAGINLPELLLKLRQRVVESYGQPFVQKFIFERILANPKLFNS
LLDLGRKFQGLIAENGEIKSLPFGLDNLTSFRVFPALAKERLYKKYRVRPKKKGTRGKVLFYSGCLIEHMYPDIGEDVFL
VLEKNGYEVVLPEDLGCCGAPAYYSGDLQAAVRMAKNNIEAFLKHEYDYIVTACPTCSETLMHYEKYLEDDEEYREKARE
VSRKVIDFTDFAYKYLAVGEGKLAGSYTLHDSCHARRGLGQVKEPRELIVKTGANLVEMANSDQCCGFGGSFSIKYPEIS
EKVFQNKYQSIMETGAEKVAVTCPGCLMQIAGGLKKQGAPVKVEHLATILAKTFRDGSENHHFLR

Specific function: Is essential for L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate. May also allow cells to utilize an alternative carbon source during biofi

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1786498, Length=470, Percent_Identity=35.9574468085106, Blast_Score=286, Evalue=5e-78,
Organism=Escherichia coli, GI1786497, Length=235, Percent_Identity=35.7446808510638, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI48994913, Length=399, Percent_Identity=24.5614035087719, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1788576, Length=419, Percent_Identity=24.3436754176611, Blast_Score=96, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825 [H]

Pfam domain/function: PF02589 DUF162 [H]

EC number: NA

Molecular weight: Translated: 78628; Mature: 78497

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00028 ZINC_FINGER_C2H2_1 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKEKIKKALGNPTLRRALTNFGNNYVLAREKVFAGLDFEELRREIKAARTEVRKKLDF
CCCHHHHHHHHCCHHHHHHHHHCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
YVEQFRINAEKNGARVIIVHSAAEAKKAIVDILKAKNAKTVVKSKSMASEEIHLNEELVK
HHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHCCHHHHH
HGIEVVETDLGEWILQLDGQKPSHMVMPAIHLTREEVAAIFSRALDREVAPDIAQMVKLA
CCHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RQELRDKFLKADVGITGGNIAVAETGSIFLFTNEGNARLTTTLPRVRISLVGVEKIVPTL
HHHHHHHHHHHCCCCCCCCEEEEECCEEEEEECCCCCEEEEECCCCEEEEHHHHHHHHHH
KDAIPIMRALPRNATAQKITSYVTVMTAGAGQEHYVILMDNGRIELKDDPVFAELFQCIR
HHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCEEEEEEECCEEEECCCCHHHHHHHHHH
CAGCLNVCPVYQQVGGHVFGHVYTGPIGTLLTAFFHGLDAAKDPQALCGSCLRCATVCPA
HHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
GINLPELLLKLRQRVVESYGQPFVQKFIFERILANPKLFNSLLDLGRKFQGLIAENGEIK
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEE
SLPFGLDNLTSFRVFPALAKERLYKKYRVRPKKKGTRGKVLFYSGCLIEHMYPDIGEDVF
ECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCEE
LVLEKNGYEVVLPEDLGCCGAPAYYSGDLQAAVRMAKNNIEAFLKHEYDYIVTACPTCSE
EEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
TLMHYEKYLEDDEEYREKAREVSRKVIDFTDFAYKYLAVGEGKLAGSYTLHDSCHARRGL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCHHHHHHCC
GQVKEPRELIVKTGANLVEMANSDQCCGFGGSFSIKYPEISEKVFQNKYQSIMETGAEKV
CCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEE
AVTCPGCLMQIAGGLKKQGAPVKVEHLATILAKTFRDGSENHHFLR
EEECCHHHHHHHCCHHCCCCCEEHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ANKEKIKKALGNPTLRRALTNFGNNYVLAREKVFAGLDFEELRREIKAARTEVRKKLDF
CCHHHHHHHHCCHHHHHHHHHCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
YVEQFRINAEKNGARVIIVHSAAEAKKAIVDILKAKNAKTVVKSKSMASEEIHLNEELVK
HHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHCCHHHHH
HGIEVVETDLGEWILQLDGQKPSHMVMPAIHLTREEVAAIFSRALDREVAPDIAQMVKLA
CCHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RQELRDKFLKADVGITGGNIAVAETGSIFLFTNEGNARLTTTLPRVRISLVGVEKIVPTL
HHHHHHHHHHHCCCCCCCCEEEEECCEEEEEECCCCCEEEEECCCCEEEEHHHHHHHHHH
KDAIPIMRALPRNATAQKITSYVTVMTAGAGQEHYVILMDNGRIELKDDPVFAELFQCIR
HHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCEEEEEEECCEEEECCCCHHHHHHHHHH
CAGCLNVCPVYQQVGGHVFGHVYTGPIGTLLTAFFHGLDAAKDPQALCGSCLRCATVCPA
HHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
GINLPELLLKLRQRVVESYGQPFVQKFIFERILANPKLFNSLLDLGRKFQGLIAENGEIK
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEE
SLPFGLDNLTSFRVFPALAKERLYKKYRVRPKKKGTRGKVLFYSGCLIEHMYPDIGEDVF
ECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCEE
LVLEKNGYEVVLPEDLGCCGAPAYYSGDLQAAVRMAKNNIEAFLKHEYDYIVTACPTCSE
EEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
TLMHYEKYLEDDEEYREKAREVSRKVIDFTDFAYKYLAVGEGKLAGSYTLHDSCHARRGL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCHHHHHHCC
GQVKEPRELIVKTGANLVEMANSDQCCGFGGSFSIKYPEISEKVFQNKYQSIMETGAEKV
CCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEE
AVTCPGCLMQIAGGLKKQGAPVKVEHLATILAKTFRDGSENHHFLR
EEECCHHHHHHHCCHHCCCCCEEHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]