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The map label for this gene is patA [H]

Identifier: 78043849

GI number: 78043849

Start: 1282379

End: 1283752

Strand: Direct

Name: patA [H]

Synonym: CHY_1436

Alternate gene names: 78043849

Gene position: 1282379-1283752 (Clockwise)

Preceding gene: 78044518

Following gene: 78044275

Centisome position: 53.4

GC content: 41.92

Gene sequence:

>1374_bases
ATGGAACTTATTACCTTGGAGCAAGCTTTAACCTTAACCCGTCAGGATATCCGGGACCTCTACCGTGAGTACGTTAACGA
AAGTTTAGCAACCCTTTTAAGTTTATTGGAATTTGATAAACAGTTTGTTAAAGCTAAGGGAACAAAAGTTTGGGACAATG
AGGGAAATATTTATCTCGACTTTTTAGGAGGTTATGGTAGCTTAAACTTCGGTCACAATCCAGAGGAAATCTGGGAAGCG
GTAAAAATGGTGGAAGAACTGCCCAATCTTTTGCAGGCCTCGATAGGTCAGTTAATTGGAGCAGCTGCCTACAATTTAGC
AAAAATAACTCCTGGTAATTTAAAGCGGGTATTTTTTGGTAATTCTGGAGCAGAAGCTGTGGAAGGAGCGCTGAAGCTCG
CCAGAATTTATACCGGACGTCCGGGCATTGTTTATGCTCACAATTCTTTTCACGGAAAATCTTTTGGCGCCCTTTCCGTT
ACCGGTAGGCAAAAGTACCAAACACCTTTTACCCCCCTCTTACCCGAATGCTATCCCGTAACATACGGAGATTTAGACGA
ATTGGAGAATATCTTAAAATCCAAACCCATCGCAGCCTTTATTGTAGAACCAATTCAAGGTGAGGGTGGCGTTGTTGTTC
CTCCGCAAGGCTATTTAAAAAATGCCTTAGAACTTTGCCATAAATACGGAGCACTGTTAATAGTGGATGAAATCCAAACC
GGTTTTGGTAGAACTGGTAAAGTCTTTGCGGTAGAATATGACGAAATTGTCCCCGATATCATGTGTGTGGCTAAATCCCT
GGGCGGTGGAGTTATGCCTGTGGGTGCGTATATAACCACTGATACTATCTGGAAAAAAGCTTACGGTTCCACCGATAAAG
CAACCCTGCATACTTCTACTTTTGGGGGAAATACTAAGGCAATGGCAGCAGTGATCAAAGCAATTGAGCTTTTAGTGAAG
TGGGATTTAGCAAAAAAAGCCAAAGAACTTGGGGATTACTTATTGAGCCAGTTAAGTTCACTGCAAACCTCCTATCCCCT
GATTAAAGAAGTCCGAGGTAAAGGTCTTTTAATTGGGCTCGAATTCAACGAACCCCAGGGCTTATTAAATAAAATTCCCG
GGCTAACAAATCTCGCCCGGGAGTATACCGGCAGCTTTGTCGCTGGACTGTTAATGAATAAGCACAGGATTATTACCGCA
TATACCCTGAATAATCCTAATGTAATTCGCTTAGAACCACCCCTTATTGTAGAAAAAGAAGAATTGGACAGGTTAATCTA
TGCTTTAAAGGATATTTTTGAAAGCCACAGTAGTTTTTTAGGGGTAGCATCGGCCAACCTTAAAACGGTTATCGGCTCCC
TTTTTAAGAGATAA

Upstream 100 bases:

>100_bases
TTCATCAATCATTTTTTCACCTGTCAAGTATTTAACCTTTACAGTAGTATTTTATTTATATATACTTAAATTGTCAAGGA
AAAAAGAAGGAGGCAAGAAA

Downstream 100 bases:

>100_bases
CTTGTAAAATGAAAAACTTCAAATAATACGTTTCGGGTGAGGAGAGGAGCATCGGGTGATCTTTAGCTTGCCGCCGGGCT
TCAATAATCCTCACTCTTTT

Product: aminotransferase, class III

Products: NA

Alternate protein names: Putrescine--2-oxoglutaric acid transaminase; PAT; PATase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MELITLEQALTLTRQDIRDLYREYVNESLATLLSLLEFDKQFVKAKGTKVWDNEGNIYLDFLGGYGSLNFGHNPEEIWEA
VKMVEELPNLLQASIGQLIGAAAYNLAKITPGNLKRVFFGNSGAEAVEGALKLARIYTGRPGIVYAHNSFHGKSFGALSV
TGRQKYQTPFTPLLPECYPVTYGDLDELENILKSKPIAAFIVEPIQGEGGVVVPPQGYLKNALELCHKYGALLIVDEIQT
GFGRTGKVFAVEYDEIVPDIMCVAKSLGGGVMPVGAYITTDTIWKKAYGSTDKATLHTSTFGGNTKAMAAVIKAIELLVK
WDLAKKAKELGDYLLSQLSSLQTSYPLIKEVRGKGLLIGLEFNEPQGLLNKIPGLTNLAREYTGSFVAGLLMNKHRIITA
YTLNNPNVIRLEPPLIVEKEELDRLIYALKDIFESHSSFLGVASANLKTVIGSLFKR

Sequences:

>Translated_457_residues
MELITLEQALTLTRQDIRDLYREYVNESLATLLSLLEFDKQFVKAKGTKVWDNEGNIYLDFLGGYGSLNFGHNPEEIWEA
VKMVEELPNLLQASIGQLIGAAAYNLAKITPGNLKRVFFGNSGAEAVEGALKLARIYTGRPGIVYAHNSFHGKSFGALSV
TGRQKYQTPFTPLLPECYPVTYGDLDELENILKSKPIAAFIVEPIQGEGGVVVPPQGYLKNALELCHKYGALLIVDEIQT
GFGRTGKVFAVEYDEIVPDIMCVAKSLGGGVMPVGAYITTDTIWKKAYGSTDKATLHTSTFGGNTKAMAAVIKAIELLVK
WDLAKKAKELGDYLLSQLSSLQTSYPLIKEVRGKGLLIGLEFNEPQGLLNKIPGLTNLAREYTGSFVAGLLMNKHRIITA
YTLNNPNVIRLEPPLIVEKEELDRLIYALKDIFESHSSFLGVASANLKTVIGSLFKR
>Mature_457_residues
MELITLEQALTLTRQDIRDLYREYVNESLATLLSLLEFDKQFVKAKGTKVWDNEGNIYLDFLGGYGSLNFGHNPEEIWEA
VKMVEELPNLLQASIGQLIGAAAYNLAKITPGNLKRVFFGNSGAEAVEGALKLARIYTGRPGIVYAHNSFHGKSFGALSV
TGRQKYQTPFTPLLPECYPVTYGDLDELENILKSKPIAAFIVEPIQGEGGVVVPPQGYLKNALELCHKYGALLIVDEIQT
GFGRTGKVFAVEYDEIVPDIMCVAKSLGGGVMPVGAYITTDTIWKKAYGSTDKATLHTSTFGGNTKAMAAVIKAIELLVK
WDLAKKAKELGDYLLSQLSSLQTSYPLIKEVRGKGLLIGLEFNEPQGLLNKIPGLTNLAREYTGSFVAGLLMNKHRIITA
YTLNNPNVIRLEPPLIVEKEELDRLIYALKDIFESHSSFLGVASANLKTVIGSLFKR

Specific function: Catalyzes the aminotransferase reaction from putrescine to 2-oxoglutarate, leading to glutamate and 4-aminobutanal, which spontaneously cyclizes to form 1-pyrroline. Is also able to transaminate cadaverine and, in lower extent, spermidine, but not ornithi

COG id: COG4992

COG function: function code E; Ornithine/acetylornithine aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. Putrescine aminotransferase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=392, Percent_Identity=34.6938775510204, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI284507298, Length=310, Percent_Identity=35.8064516129032, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI13994255, Length=471, Percent_Identity=27.8131634819533, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI37574042, Length=422, Percent_Identity=27.9620853080569, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI226442705, Length=422, Percent_Identity=27.7251184834123, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI226442709, Length=394, Percent_Identity=28.4263959390863, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI24119277, Length=417, Percent_Identity=26.8585131894484, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI188536080, Length=240, Percent_Identity=26.6666666666667, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI38679950, Length=240, Percent_Identity=26.6666666666667, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI38679946, Length=240, Percent_Identity=26.6666666666667, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI145693181, Length=445, Percent_Identity=39.3258426966292, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1788044, Length=408, Percent_Identity=34.5588235294118, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1789759, Length=413, Percent_Identity=31.2348668280872, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1787560, Length=411, Percent_Identity=33.3333333333333, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1789016, Length=341, Percent_Identity=31.0850439882698, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786991, Length=422, Percent_Identity=28.1990521327014, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1786349, Length=342, Percent_Identity=29.5321637426901, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25144271, Length=431, Percent_Identity=33.1786542923434, Blast_Score=207, Evalue=9e-54,
Organism=Caenorhabditis elegans, GI25144274, Length=414, Percent_Identity=30.4347826086957, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI32564660, Length=430, Percent_Identity=30.9302325581395, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71992977, Length=452, Percent_Identity=26.1061946902655, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17541228, Length=398, Percent_Identity=25.3768844221106, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71981843, Length=203, Percent_Identity=29.064039408867, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=409, Percent_Identity=37.1638141809291, Blast_Score=213, Evalue=7e-56,
Organism=Saccharomyces cerevisiae, GI6324432, Length=418, Percent_Identity=33.4928229665072, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324386, Length=379, Percent_Identity=25.8575197889182, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321456, Length=447, Percent_Identity=25.2796420581655, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357415, Length=394, Percent_Identity=32.48730964467, Blast_Score=197, Evalue=9e-51,
Organism=Drosophila melanogaster, GI161085790, Length=448, Percent_Identity=30.8035714285714, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI21356575, Length=422, Percent_Identity=29.6208530805687, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI28574759, Length=427, Percent_Identity=31.1475409836066, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI281366494, Length=385, Percent_Identity=24.1558441558442, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24667139, Length=385, Percent_Identity=24.1558441558442, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24667143, Length=385, Percent_Identity=24.1558441558442, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR010164
- InterPro:   IPR017747
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.82 [H]

Molecular weight: Translated: 50199; Mature: 50199

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELITLEQALTLTRQDIRDLYREYVNESLATLLSLLEFDKQFVKAKGTKVWDNEGNIYLD
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEE
FLGGYGSLNFGHNPEEIWEAVKMVEELPNLLQASIGQLIGAAAYNLAKITPGNLKRVFFG
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEC
NSGAEAVEGALKLARIYTGRPGIVYAHNSFHGKSFGALSVTGRQKYQTPFTPLLPECYPV
CCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCC
TYGDLDELENILKSKPIAAFIVEPIQGEGGVVVPPQGYLKNALELCHKYGALLIVDEIQT
CCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCHHHHHHHHHHHHCCCEEEEECHHC
GFGRTGKVFAVEYDEIVPDIMCVAKSLGGGVMPVGAYITTDTIWKKAYGSTDKATLHTST
CCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEECCCEEEHHHHHHHHCCCCCCCEEEEEC
FGGNTKAMAAVIKAIELLVKWDLAKKAKELGDYLLSQLSSLQTSYPLIKEVRGKGLLIGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEE
EFNEPQGLLNKIPGLTNLAREYTGSFVAGLLMNKHRIITAYTLNNPNVIRLEPPLIVEKE
ECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCCEECHH
ELDRLIYALKDIFESHSSFLGVASANLKTVIGSLFKR
HHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MELITLEQALTLTRQDIRDLYREYVNESLATLLSLLEFDKQFVKAKGTKVWDNEGNIYLD
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEE
FLGGYGSLNFGHNPEEIWEAVKMVEELPNLLQASIGQLIGAAAYNLAKITPGNLKRVFFG
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEC
NSGAEAVEGALKLARIYTGRPGIVYAHNSFHGKSFGALSVTGRQKYQTPFTPLLPECYPV
CCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCC
TYGDLDELENILKSKPIAAFIVEPIQGEGGVVVPPQGYLKNALELCHKYGALLIVDEIQT
CCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCHHHHHHHHHHHHCCCEEEEECHHC
GFGRTGKVFAVEYDEIVPDIMCVAKSLGGGVMPVGAYITTDTIWKKAYGSTDKATLHTST
CCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEECCCEEEHHHHHHHHCCCCCCCEEEEEC
FGGNTKAMAAVIKAIELLVKWDLAKKAKELGDYLLSQLSSLQTSYPLIKEVRGKGLLIGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEE
EFNEPQGLLNKIPGLTNLAREYTGSFVAGLLMNKHRIITAYTLNNPNVIRLEPPLIVEKE
ECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCCEECHH
ELDRLIYALKDIFESHSSFLGVASANLKTVIGSLFKR
HHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA