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The map label for this gene is holB [H]

Identifier: 78043743

GI number: 78043743

Start: 41486

End: 42289

Strand: Direct

Name: holB [H]

Synonym: CHY_0051

Alternate gene names: 78043743

Gene position: 41486-42289 (Clockwise)

Preceding gene: 78044441

Following gene: 78042958

Centisome position: 1.73

GC content: 41.42

Gene sequence:

>804_bases
ATGGATAATCTAATTATAGAAGCTTTAAATCAGGATAAGCTAAGTCATGCGTATTTATTAACTTCACCGGTTCTTGAGGC
GGTAGTGGATTTTGCCCGAAGGACCGCCCGGGAAATCTTAATAAAATCGGCCAATGACCCCGTGGAGGCAGCAAACCTTT
TTGAGGCGGGAACTCATCCGGATTTTCTCTGGCTTGCACCCGATGGGAGCTCCTTTAAAATCTGGCAGGTCAATGAAGTG
TTGATTCCCAAAGTTTACCTTACTCCCCAAAAGGGCAGCCGCAAGGTAATTGTTTTAGAAGAAGTAGATAAAATGACCCT
TGATGCCGCCAATGCTTTTTTAAAGACTTTAGAGGAGCCGCCGGCAGGGGTTACTTACCTTTTAATTGCTACCATGCCGG
AAAAAGTGCTTCCGACAATTCATTCCCGTTGCCAAAAAGTTAAAGTTTCAGCCGAAGATTTTACAGGCCAGTATAAAGGG
TATGCGGAAAAGTTTTATGAGCTTTTAACTTTGCCCTGGGAGGAGTTTTATAAAAAGACGGAAAAGATTACCCGGGAAGA
GCTAAATAATCTTTTGCTGGCAGGAGAATTAATTCTCCGGGATGCGCTGATTGTTGATAAAGGATCGGAAAAATTGTTTT
ATCCGGATCTTTTCGATAAAGCACGGGAATTAAACCGTTTAGGTGAAGAGGAAATTTACCGTTTACTTAATCGTTTGGAA
GATTTTTTAGCCAAAACCCGTTTAAATTTAAATCCCAGGCTTGTGTTAGAAAACCTCTACCTGAACTTTAGCTTTTACAA
GTAG

Upstream 100 bases:

>100_bases
TTTTTACAACTTGCCAGAGATCAGCGTTTTCGAGTGGTGGATGGCCGGAAAAAGCCCGAGGAAATTTTTTGGGAAATCAA
AGGGTTTTTGGAGGGGCTAA

Downstream 100 bases:

>100_bases
TAAACTTTGAAAAAATAATTTATAATAAAAACAATTATAAATTTGGAGGAGTAATATGCCAAAAGTAGTTGGGATTCGCT
TTAAAAGAGCGGGGAAGATT

Product: putative DNA polymerase III, delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MDNLIIEALNQDKLSHAYLLTSPVLEAVVDFARRTAREILIKSANDPVEAANLFEAGTHPDFLWLAPDGSSFKIWQVNEV
LIPKVYLTPQKGSRKVIVLEEVDKMTLDAANAFLKTLEEPPAGVTYLLIATMPEKVLPTIHSRCQKVKVSAEDFTGQYKG
YAEKFYELLTLPWEEFYKKTEKITREELNNLLLAGELILRDALIVDKGSEKLFYPDLFDKARELNRLGEEEIYRLLNRLE
DFLAKTRLNLNPRLVLENLYLNFSFYK

Sequences:

>Translated_267_residues
MDNLIIEALNQDKLSHAYLLTSPVLEAVVDFARRTAREILIKSANDPVEAANLFEAGTHPDFLWLAPDGSSFKIWQVNEV
LIPKVYLTPQKGSRKVIVLEEVDKMTLDAANAFLKTLEEPPAGVTYLLIATMPEKVLPTIHSRCQKVKVSAEDFTGQYKG
YAEKFYELLTLPWEEFYKKTEKITREELNNLLLAGELILRDALIVDKGSEKLFYPDLFDKARELNRLGEEEIYRLLNRLE
DFLAKTRLNLNPRLVLENLYLNFSFYK
>Mature_267_residues
MDNLIIEALNQDKLSHAYLLTSPVLEAVVDFARRTAREILIKSANDPVEAANLFEAGTHPDFLWLAPDGSSFKIWQVNEV
LIPKVYLTPQKGSRKVIVLEEVDKMTLDAANAFLKTLEEPPAGVTYLLIATMPEKVLPTIHSRCQKVKVSAEDFTGQYKG
YAEKFYELLTLPWEEFYKKTEKITREELNNLLLAGELILRDALIVDKGSEKLFYPDLFDKARELNRLGEEEIYRLLNRLE
DFLAKTRLNLNPRLVLENLYLNFSFYK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 30729; Mature: 30729

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNLIIEALNQDKLSHAYLLTSPVLEAVVDFARRTAREILIKSANDPVEAANLFEAGTHP
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
DFLWLAPDGSSFKIWQVNEVLIPKVYLTPQKGSRKVIVLEEVDKMTLDAANAFLKTLEEP
CEEEECCCCCCEEEEEECCEEECEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCC
PAGVTYLLIATMPEKVLPTIHSRCQKVKVSAEDFTGQYKGYAEKFYELLTLPWEEFYKKT
CCCEEEEEEECCCHHHHHHHHHHHHHEECCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHH
EKITREELNNLLLAGELILRDALIVDKGSEKLFYPDLFDKARELNRLGEEEIYRLLNRLE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEECCHHHHHHHHHHHCCHHHHHHHHHHHH
DFLAKTRLNLNPRLVLENLYLNFSFYK
HHHHHHHCCCCHHEEEEEEEEEEEECC
>Mature Secondary Structure
MDNLIIEALNQDKLSHAYLLTSPVLEAVVDFARRTAREILIKSANDPVEAANLFEAGTHP
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
DFLWLAPDGSSFKIWQVNEVLIPKVYLTPQKGSRKVIVLEEVDKMTLDAANAFLKTLEEP
CEEEECCCCCCEEEEEECCEEECEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCC
PAGVTYLLIATMPEKVLPTIHSRCQKVKVSAEDFTGQYKGYAEKFYELLTLPWEEFYKKT
CCCEEEEEEECCCHHHHHHHHHHHHHEECCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHH
EKITREELNNLLLAGELILRDALIVDKGSEKLFYPDLFDKARELNRLGEEEIYRLLNRLE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEECCHHHHHHHHHHHCCHHHHHHHHHHHH
DFLAKTRLNLNPRLVLENLYLNFSFYK
HHHHHHHCCCCHHEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]