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The map label for this gene is hyuA [H]

Identifier: 78043707

GI number: 78043707

Start: 226975

End: 228657

Strand: Direct

Name: hyuA [H]

Synonym: CHY_0262

Alternate gene names: 78043707

Gene position: 226975-228657 (Clockwise)

Preceding gene: 78044591

Following gene: 78043060

Centisome position: 9.45

GC content: 45.22

Gene sequence:

>1683_bases
ATGATTGTAGGCATTGATGTAGGGGGGACGTATACCGACGGAGTTGCTTTAGATCAGGGCAGGGTTGTGGCTAAGGCTAA
AGTGGTTACGAAAAAAAACCTGCCGGAAACCTTTGCTGAAGTATTAGCGAAGCTTATAGCCCAGCACAATCAGCCTATTA
AAAGGGTGGTTTTGAGCACTACCCTAATTACCAATTTGGTGGCGGAGCGAAAATACGACCGGGCGGCGGCCTTGTTGCTT
CCGGGGGCGGGGATGGATTATGAAAAAATAAACTTTCCCTTTCCGGTGAAGGTAGTTTCCGGAATGGTCGATTTTCGCGG
TCGGGTTTTAGAAGAAATTAACGAAAAGGAAGTAGAACAAGCTTTACAGGAATTTTTGGCTCAGGGGTTTAGGAAAGTAG
CGCTGGTGGGGAAGTTTTCGGTAAGAAATCCCGAACTTGAACGAAAGCTTTACCAGATTGTCAAAAAAATCAACCCGCAA
ATAAAAACCGCTCTTGGGCATAAGACCGGGGGGCGTTTGAATTATCTCCGGCGGATTTATACTTCGGTTTTATACCTGAT
GATAAAAGATGAGTTTAACCGCTTTGTTGAGGAATTAAAGGTAATCTTTTATGCTTTAAAGATCCCCTTAAACGTAGTTT
TTATCATGAAAGGAGATGGGGGGATTGTACCACTTCTTGAAGTTGAGCGTTACCCGCTGGCTACTCTTTATTCCGGCCCG
GCGGCAAGCGCATTAGGGGCTTATGCCCTCTGCAACTGCCAAAACATAGTCGTTTTGGATATTGGAGGTACCACCACCGA
TATTGCTTTAGTTTTAAACCAGCGTCCGCTTCTTTCCCATAAAGGGGCGGATATTGGGGAATTTTTAACTTCGGTACCGA
GTTTAGCAGTTACATCAGTTCCTTTTGGTGGTGATACGGCGGTTACCGTTTTGGACGGAGAAATTACCTTTACCGGACAG
CGCCAGGGTCCGGCCTACTGTCTGGGCGGTCCGGCGGTTACCGTTACCGACGCTTTAAGGTTTTTAAATTTAATAGATTA
CGGGGATTTCCAGCGGGCCAAGCGGGGAATCCACAAGCTTGCCCAGGAAACCGGGTTAACTTCCCTTGATGTGGCCCAAA
AAATTGTTGATATATTTACCGATACCGTGGTCCAGGAAGTAAAAAATACTTTTAAGCGCTGGGAAAACGAACCTCGCTAC
CGGATTTATGAAATTATGACGGGGAAGAAATTTGAACCTGAATTGCTAAGTGGAGTAGGGGGAGGAGCTTACCCGCTCAC
CTTGCTGATAGCTAAAAAACTTGGAATGAAAGGGGTAATTTATGAAAATTACGAAGTGGCCAATGCTTTAGGAGCAGCCC
TGGCCCGGGATAATCTTTCCATTACCGTTTATATCGATACCGAGCAAAAATATGTGGCCATACCGGAATGGGGTTACAAA
GAGGCCATTCCCAAACTAAAAAATTACAGCGAAGATGAGGTTAAAGAATTTGCTCTGTTTGTTTTTCGCATTGGTGCTAA
AAGGATGAACCTGCGTTATCCTCCCGGCAGTGAAGAACTAATCTACTTTGAGCAGTTTAATGTGGTGCGGGATATGGTGA
CGGAAAACAAAATATTTATTCTGGAAATAGCGGTGCCGGCCGGGATTTTACGGCCGGTAGAAGGGAGGATTTTGTATGAA
TAA

Upstream 100 bases:

>100_bases
AAAATATAAAATCTTAGAAACATTCATGTAAATTTTATTTAAGTCTTGAAAGTTTAAGTGATAAAATAAAGAAAAAAGTT
TTGTTACGGGGGAGAAAGGA

Downstream 100 bases:

>100_bases
AACCGGTCTTTTGTTTTTCCCCGCCTTTGATTGGGCCATATCCCCTACCCATCCGGAAAGGGAAGAACGGCTGTTATATA
CCCGGGACCAGATAATGGAG

Product: hydantoinase/oxoprolinase family protein

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MIVGIDVGGTYTDGVALDQGRVVAKAKVVTKKNLPETFAEVLAKLIAQHNQPIKRVVLSTTLITNLVAERKYDRAAALLL
PGAGMDYEKINFPFPVKVVSGMVDFRGRVLEEINEKEVEQALQEFLAQGFRKVALVGKFSVRNPELERKLYQIVKKINPQ
IKTALGHKTGGRLNYLRRIYTSVLYLMIKDEFNRFVEELKVIFYALKIPLNVVFIMKGDGGIVPLLEVERYPLATLYSGP
AASALGAYALCNCQNIVVLDIGGTTTDIALVLNQRPLLSHKGADIGEFLTSVPSLAVTSVPFGGDTAVTVLDGEITFTGQ
RQGPAYCLGGPAVTVTDALRFLNLIDYGDFQRAKRGIHKLAQETGLTSLDVAQKIVDIFTDTVVQEVKNTFKRWENEPRY
RIYEIMTGKKFEPELLSGVGGGAYPLTLLIAKKLGMKGVIYENYEVANALGAALARDNLSITVYIDTEQKYVAIPEWGYK
EAIPKLKNYSEDEVKEFALFVFRIGAKRMNLRYPPGSEELIYFEQFNVVRDMVTENKIFILEIAVPAGILRPVEGRILYE

Sequences:

>Translated_560_residues
MIVGIDVGGTYTDGVALDQGRVVAKAKVVTKKNLPETFAEVLAKLIAQHNQPIKRVVLSTTLITNLVAERKYDRAAALLL
PGAGMDYEKINFPFPVKVVSGMVDFRGRVLEEINEKEVEQALQEFLAQGFRKVALVGKFSVRNPELERKLYQIVKKINPQ
IKTALGHKTGGRLNYLRRIYTSVLYLMIKDEFNRFVEELKVIFYALKIPLNVVFIMKGDGGIVPLLEVERYPLATLYSGP
AASALGAYALCNCQNIVVLDIGGTTTDIALVLNQRPLLSHKGADIGEFLTSVPSLAVTSVPFGGDTAVTVLDGEITFTGQ
RQGPAYCLGGPAVTVTDALRFLNLIDYGDFQRAKRGIHKLAQETGLTSLDVAQKIVDIFTDTVVQEVKNTFKRWENEPRY
RIYEIMTGKKFEPELLSGVGGGAYPLTLLIAKKLGMKGVIYENYEVANALGAALARDNLSITVYIDTEQKYVAIPEWGYK
EAIPKLKNYSEDEVKEFALFVFRIGAKRMNLRYPPGSEELIYFEQFNVVRDMVTENKIFILEIAVPAGILRPVEGRILYE
>Mature_560_residues
MIVGIDVGGTYTDGVALDQGRVVAKAKVVTKKNLPETFAEVLAKLIAQHNQPIKRVVLSTTLITNLVAERKYDRAAALLL
PGAGMDYEKINFPFPVKVVSGMVDFRGRVLEEINEKEVEQALQEFLAQGFRKVALVGKFSVRNPELERKLYQIVKKINPQ
IKTALGHKTGGRLNYLRRIYTSVLYLMIKDEFNRFVEELKVIFYALKIPLNVVFIMKGDGGIVPLLEVERYPLATLYSGP
AASALGAYALCNCQNIVVLDIGGTTTDIALVLNQRPLLSHKGADIGEFLTSVPSLAVTSVPFGGDTAVTVLDGEITFTGQ
RQGPAYCLGGPAVTVTDALRFLNLIDYGDFQRAKRGIHKLAQETGLTSLDVAQKIVDIFTDTVVQEVKNTFKRWENEPRY
RIYEIMTGKKFEPELLSGVGGGAYPLTLLIAKKLGMKGVIYENYEVANALGAALARDNLSITVYIDTEQKYVAIPEWGYK
EAIPKLKNYSEDEVKEFALFVFRIGAKRMNLRYPPGSEELIYFEQFNVVRDMVTENKIFILEIAVPAGILRPVEGRILYE

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322634, Length=538, Percent_Identity=22.6765799256506, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 62262; Mature: 62262

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVGIDVGGTYTDGVALDQGRVVAKAKVVTKKNLPETFAEVLAKLIAQHNQPIKRVVLST
CEEEEECCCCCCCCEEECCCCEEEEEEEHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
TLITNLVAERKYDRAAALLLPGAGMDYEKINFPFPVKVVSGMVDFRGRVLEEINEKEVEQ
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
ALQEFLAQGFRKVALVGKFSVRNPELERKLYQIVKKINPQIKTALGHKTGGRLNYLRRIY
HHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHH
TSVLYLMIKDEFNRFVEELKVIFYALKIPLNVVFIMKGDGGIVPLLEVERYPLATLYSGP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCEEEEECCC
AASALGAYALCNCQNIVVLDIGGTTTDIALVLNQRPLLSHKGADIGEFLTSVPSLAVTSV
CHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCEEEEC
PFGGDTAVTVLDGEITFTGQRQGPAYCLGGPAVTVTDALRFLNLIDYGDFQRAKRGIHKL
CCCCCEEEEEEECEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
AQETGLTSLDVAQKIVDIFTDTVVQEVKNTFKRWENEPRYRIYEIMTGKKFEPELLSGVG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHCCC
GGAYPLTLLIAKKLGMKGVIYENYEVANALGAALARDNLSITVYIDTEQKYVAIPEWGYK
CCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCCHH
EAIPKLKNYSEDEVKEFALFVFRIGAKRMNLRYPPGSEELIYFEQFNVVRDMVTENKIFI
HHHHHHHCCCHHHHHHHHHHHHHHCHHHEEEECCCCCCCEEEHHHHHHHHHHHCCCCEEE
LEIAVPAGILRPVEGRILYE
EEEECCCCCCCCCCCCEECC
>Mature Secondary Structure
MIVGIDVGGTYTDGVALDQGRVVAKAKVVTKKNLPETFAEVLAKLIAQHNQPIKRVVLST
CEEEEECCCCCCCCEEECCCCEEEEEEEHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
TLITNLVAERKYDRAAALLLPGAGMDYEKINFPFPVKVVSGMVDFRGRVLEEINEKEVEQ
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
ALQEFLAQGFRKVALVGKFSVRNPELERKLYQIVKKINPQIKTALGHKTGGRLNYLRRIY
HHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHH
TSVLYLMIKDEFNRFVEELKVIFYALKIPLNVVFIMKGDGGIVPLLEVERYPLATLYSGP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCEEEEECCC
AASALGAYALCNCQNIVVLDIGGTTTDIALVLNQRPLLSHKGADIGEFLTSVPSLAVTSV
CHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCEEEEC
PFGGDTAVTVLDGEITFTGQRQGPAYCLGGPAVTVTDALRFLNLIDYGDFQRAKRGIHKL
CCCCCEEEEEEECEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
AQETGLTSLDVAQKIVDIFTDTVVQEVKNTFKRWENEPRYRIYEIMTGKKFEPELLSGVG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHCCC
GGAYPLTLLIAKKLGMKGVIYENYEVANALGAALARDNLSITVYIDTEQKYVAIPEWGYK
CCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCCHH
EAIPKLKNYSEDEVKEFALFVFRIGAKRMNLRYPPGSEELIYFEQFNVVRDMVTENKIFI
HHHHHHHCCCHHHHHHHHHHHHHHCHHHEEEECCCCCCCEEEHHHHHHHHHHHCCCCEEE
LEIAVPAGILRPVEGRILYE
EEEECCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]