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The map label for this gene is pmbA [H]

Identifier: 78043700

GI number: 78043700

Start: 568255

End: 569616

Strand: Direct

Name: pmbA [H]

Synonym: CHY_0656

Alternate gene names: 78043700

Gene position: 568255-569616 (Clockwise)

Preceding gene: 78044916

Following gene: 78043390

Centisome position: 23.66

GC content: 47.21

Gene sequence:

>1362_bases
ATGGTGGCTGTAAGTACCGTCCCTGGAGGAGGTATTTTAGTGGAGATTCTGGAACTGGCGTTAGCGGAAATTAAAGCGAA
AAAGGCTGATGGGGAAGTTTGTTATACCCGGGGCGAAGAGCTTTCGGTGGAAGTGCGGGAAGGACGGGTGGAGACGGTTA
AGCAGGCGGTAGAAGAAGGGGTAGGGGTACGGGTCTTTTACGAGGGAAGGCTTGGCTTTGCCTATACCACGGTAATGACT
CCGGAAAAAATCAAGGAAACGGTGGAAAAAGCAGTCCAGGCGGCAAAGATTACCGATAGCGATCCTTACTTACGTTTGGC
AAAACCTTCTACCTACCCAACGCCGGAGTTAACCGACCCGGAAATAACCACTGCTTCCTTGGAGGAAAAGGTTAATTTAG
CACTTACCATGGAAAGAGAAGCGTTAAATTTCAGCCCCAAAATTAAGGGGATTGAAACTGCGGCCTATGATGACTACCAT
TATCAATGGCATTTAGCCAATACCGAAGGTTTTTTCGGAAGTTTTTCGGGAAATTATGCTTCAATTTATATTTATTTAGC
TTCGGAAGATGAATCGGGCTCGGAGACCGCTTTTTCCTATAAAGTAACCCGCAAGTTTAAGGAAATAGACCCGAAAGCTG
TAGGCCGGGAAGCGGCGGAAAAAGCGGTAGCCAAACTTGGGGCCGGTAAGGTTCCCTCCGGACGGTATATGGCACTTTTA
GACCCGTATGTGGCCGTAAATCTTTTAGGGGTGTTAAAAGCCTCGTTCTTAGCGGAAAATGTGCAAAAAGGTAAAAGCCT
TTTAAAAGGGAAACTTGGCGAAAAAGTGTTTAGCCCCGTTTTCAGCTTAATTGATGATGGTACCCTTAAAGATGGCCTGG
CTACTGCACCGTTTGACGGGGAAGGGGTGCCTACCCGGAAAACCGTTTTGGTAGAAAACGGTACTGTTAAAAGCTTTCTT
TATAATTTATACACCGCCGCCAAAGAAGGAGCGGTTTCAACCGGCAATGCCGTTCGCTCTTCCTTTAAAGCTTTACCGGA
GCTGGGGACCACTAATTTCTACATTCCTCGGGGAGGAGTTAGCCGGGAAAGTTTATTTAAACAAACCAGCCGGGGGGTTT
ATATTACCGAAGTGCTCGGGATGCATACCGCAAACCCCGTTTCCGGTGATTTTTCGGTAGGAGCGACCGGCTTTTTAGTA
GAAAATGGCGAAATTATCCGGCCGGTCAGGGGGATAACCATTGCCGGGAATATAAAAGATTTATTTGGAAAATTAATTGC
TGTGGGCGATGACCTGCGCTTTTACGGAGCTAAAGGAAGTCCGACCCTGTTAATTGAAGAGGTGGCGGTAAGCGGAAGTT
AG

Upstream 100 bases:

>100_bases
GGCAAAAAATATACCTTAGAAGTAACCTTGGGCGAAAAACCAAGAGAATAACGCTGGAGGGACGGTTCTGAGAACCACCA
CTTTCGACGATGAATAGAAA

Downstream 100 bases:

>100_bases
AGGGTTGAAACCCTCTTTTTTTTAAATTCTTTCCCGGTATAAAAATTTGTTTTTTCCTCAAAAAAAATGCATATTTATTT
TTTTAAATTTTAAATTATAA

Product: putative pmbA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MVAVSTVPGGGILVEILELALAEIKAKKADGEVCYTRGEELSVEVREGRVETVKQAVEEGVGVRVFYEGRLGFAYTTVMT
PEKIKETVEKAVQAAKITDSDPYLRLAKPSTYPTPELTDPEITTASLEEKVNLALTMEREALNFSPKIKGIETAAYDDYH
YQWHLANTEGFFGSFSGNYASIYIYLASEDESGSETAFSYKVTRKFKEIDPKAVGREAAEKAVAKLGAGKVPSGRYMALL
DPYVAVNLLGVLKASFLAENVQKGKSLLKGKLGEKVFSPVFSLIDDGTLKDGLATAPFDGEGVPTRKTVLVENGTVKSFL
YNLYTAAKEGAVSTGNAVRSSFKALPELGTTNFYIPRGGVSRESLFKQTSRGVYITEVLGMHTANPVSGDFSVGATGFLV
ENGEIIRPVRGITIAGNIKDLFGKLIAVGDDLRFYGAKGSPTLLIEEVAVSGS

Sequences:

>Translated_453_residues
MVAVSTVPGGGILVEILELALAEIKAKKADGEVCYTRGEELSVEVREGRVETVKQAVEEGVGVRVFYEGRLGFAYTTVMT
PEKIKETVEKAVQAAKITDSDPYLRLAKPSTYPTPELTDPEITTASLEEKVNLALTMEREALNFSPKIKGIETAAYDDYH
YQWHLANTEGFFGSFSGNYASIYIYLASEDESGSETAFSYKVTRKFKEIDPKAVGREAAEKAVAKLGAGKVPSGRYMALL
DPYVAVNLLGVLKASFLAENVQKGKSLLKGKLGEKVFSPVFSLIDDGTLKDGLATAPFDGEGVPTRKTVLVENGTVKSFL
YNLYTAAKEGAVSTGNAVRSSFKALPELGTTNFYIPRGGVSRESLFKQTSRGVYITEVLGMHTANPVSGDFSVGATGFLV
ENGEIIRPVRGITIAGNIKDLFGKLIAVGDDLRFYGAKGSPTLLIEEVAVSGS
>Mature_453_residues
MVAVSTVPGGGILVEILELALAEIKAKKADGEVCYTRGEELSVEVREGRVETVKQAVEEGVGVRVFYEGRLGFAYTTVMT
PEKIKETVEKAVQAAKITDSDPYLRLAKPSTYPTPELTDPEITTASLEEKVNLALTMEREALNFSPKIKGIETAAYDDYH
YQWHLANTEGFFGSFSGNYASIYIYLASEDESGSETAFSYKVTRKFKEIDPKAVGREAAEKAVAKLGAGKVPSGRYMALL
DPYVAVNLLGVLKASFLAENVQKGKSLLKGKLGEKVFSPVFSLIDDGTLKDGLATAPFDGEGVPTRKTVLVENGTVKSFL
YNLYTAAKEGAVSTGNAVRSSFKALPELGTTNFYIPRGGVSRESLFKQTSRGVYITEVLGMHTANPVSGDFSVGATGFLV
ENGEIIRPVRGITIAGNIKDLFGKLIAVGDDLRFYGAKGSPTLLIEEVAVSGS

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=445, Percent_Identity=28.7640449438202, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1789640, Length=422, Percent_Identity=23.2227488151659, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48716; Mature: 48716

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVSTVPGGGILVEILELALAEIKAKKADGEVCYTRGEELSVEVREGRVETVKQAVEEG
CEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHCC
VGVRVFYEGRLGFAYTTVMTPEKIKETVEKAVQAAKITDSDPYLRLAKPSTYPTPELTDP
CCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCC
EITTASLEEKVNLALTMEREALNFSPKIKGIETAAYDDYHYQWHLANTEGFFGSFSGNYA
CCCHHHHHCCCEEEEEEEHHHCCCCCCCCCCEEEEECCEEEEEEEECCCCCCCCCCCCEE
SIYIYLASEDESGSETAFSYKVTRKFKEIDPKAVGREAAEKAVAKLGAGKVPSGRYMALL
EEEEEEECCCCCCCCEEEEEHHHHHHHHCCHHHHCHHHHHHHHHHHCCCCCCCCCEEEEE
DPYVAVNLLGVLKASFLAENVQKGKSLLKGKLGEKVFSPVFSLIDDGTLKDGLATAPFDG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCC
EGVPTRKTVLVENGTVKSFLYNLYTAAKEGAVSTGNAVRSSFKALPELGTTNFYIPRGGV
CCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCC
SRESLFKQTSRGVYITEVLGMHTANPVSGDFSVGATGFLVENGEIIRPVRGITIAGNIKD
CHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCEECCCCCEEEECCHHH
LFGKLIAVGDDLRFYGAKGSPTLLIEEVAVSGS
HHHHHHHCCCCEEEEECCCCCEEEEEEEECCCC
>Mature Secondary Structure
MVAVSTVPGGGILVEILELALAEIKAKKADGEVCYTRGEELSVEVREGRVETVKQAVEEG
CEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHCC
VGVRVFYEGRLGFAYTTVMTPEKIKETVEKAVQAAKITDSDPYLRLAKPSTYPTPELTDP
CCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCC
EITTASLEEKVNLALTMEREALNFSPKIKGIETAAYDDYHYQWHLANTEGFFGSFSGNYA
CCCHHHHHCCCEEEEEEEHHHCCCCCCCCCCEEEEECCEEEEEEEECCCCCCCCCCCCEE
SIYIYLASEDESGSETAFSYKVTRKFKEIDPKAVGREAAEKAVAKLGAGKVPSGRYMALL
EEEEEEECCCCCCCCEEEEEHHHHHHHHCCHHHHCHHHHHHHHHHHCCCCCCCCCEEEEE
DPYVAVNLLGVLKASFLAENVQKGKSLLKGKLGEKVFSPVFSLIDDGTLKDGLATAPFDG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCC
EGVPTRKTVLVENGTVKSFLYNLYTAAKEGAVSTGNAVRSSFKALPELGTTNFYIPRGGV
CCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCC
SRESLFKQTSRGVYITEVLGMHTANPVSGDFSVGATGFLVENGEIIRPVRGITIAGNIKD
CHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCEECCCCCEEEECCHHH
LFGKLIAVGDDLRFYGAKGSPTLLIEEVAVSGS
HHHHHHHCCCCEEEEECCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]