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The map label for this gene is gutB [H]

Identifier: 78043661

GI number: 78043661

Start: 1164148

End: 1165185

Strand: Direct

Name: gutB [H]

Synonym: CHY_1307

Alternate gene names: 78043661

Gene position: 1164148-1165185 (Clockwise)

Preceding gene: 78043034

Following gene: 78044568

Centisome position: 48.48

GC content: 39.6

Gene sequence:

>1038_bases
ATGAAAGCTGCGGTATTAATGGAGCCGAAAAAAATAATTTTTAAAGAAGTCCCAGTACCAGAACTGGAAAAAGGTAAGGT
GCTTATTAAAGTAGAGGCGGTTGGCATTTGTGGGAGCGATATGCATCTTTATCTTGACGGGCACATAGGGGCAACAGTTC
TGGATAAACCATTAGTGTTAGGACATGAAATCGTAGGCACGGTTATTGAAGTGGGTGAAGGAGTTAATAGAGAATTATTA
GGCCAAAGAGTAATTGTTGATCCTGGTGAAAATTGTGGACAATGTGAGCACTGTCGGACGGGGGCTTATAACCTGTGTTC
ATTTTCCAAATTTAAAGGTATTCCACCAGTAGATGGTGGAATGGCGGAGTATATAACAGCATTGGCTACGCATGTTATAC
CGCTACCTGAAAACCTTGATTCACCAACAGCTACGTTATTAGAACCATTTAGTGTTGGTTTACAAGCTGTAGATGTGGCT
GATTTTCGAGCTGGAGCTAAAATTGCAGTTTTAGGTGGAGGACCTGTAGGAGTGTTAACAGCAATTGCTGCAAAAATTAG
AGGGTGTGGTGACTTATGGCTAACTGAATTATACGAAAGAAGAATAGAAATTGCCCGAAAATTGGGAATAGAAAAGGTAA
TCAACGTAGCTCAAGAGGACCCATTGAAAACAATAATGGAAGTCACTAAAAAATCAGGTGTGGATGTTGTTTTTGAAACT
ACTGGAAATCCTAAAGCTGTCGAACAAGCGCTTCAGATTGTAAAACGGGGAGGTACGGTGGTATTTCTGGGGATTGGTGC
AGGAAAAGTCCCTATTAATATTGATCAAGTGACAAGAACAGGTCTTAAACTTTTGGGGAGTTTTCGCTATCAATATCACT
TTGCTGGAGCAGTTGCCTTGGTAAAAAAACATAACCTAGACTTTTCTCCCCTTGTCACCAATATCTACGACTTTGTAGAT
ATACCTAAAGCTTTAGAAGAAGTTTCAACTTATAAAGACAGAATTATGAAGGGAGTAATAGTCTTTCCCCAAAAATAA

Upstream 100 bases:

>100_bases
CCCCTGAAGAGGTTGCCGAAGCGATTTTGTTTTTAGTTTCCCCTAAAGCTTCGTATATAACTGGAATTAATTTGCTGGTG
GATGGAGGCTGGACAGCCTA

Downstream 100 bases:

>100_bases
ATTGTTTGAGGTGAATGTATGAGTAAAAAAATTAAAGTACGATTTTTACCGAAAGGCCGTGGTCCCAAACATGGAGCGCC
GGTAACTATCGCGTATGTTG

Product: sorbitol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKAAVLMEPKKIIFKEVPVPELEKGKVLIKVEAVGICGSDMHLYLDGHIGATVLDKPLVLGHEIVGTVIEVGEGVNRELL
GQRVIVDPGENCGQCEHCRTGAYNLCSFSKFKGIPPVDGGMAEYITALATHVIPLPENLDSPTATLLEPFSVGLQAVDVA
DFRAGAKIAVLGGGPVGVLTAIAAKIRGCGDLWLTELYERRIEIARKLGIEKVINVAQEDPLKTIMEVTKKSGVDVVFET
TGNPKAVEQALQIVKRGGTVVFLGIGAGKVPINIDQVTRTGLKLLGSFRYQYHFAGAVALVKKHNLDFSPLVTNIYDFVD
IPKALEEVSTYKDRIMKGVIVFPQK

Sequences:

>Translated_345_residues
MKAAVLMEPKKIIFKEVPVPELEKGKVLIKVEAVGICGSDMHLYLDGHIGATVLDKPLVLGHEIVGTVIEVGEGVNRELL
GQRVIVDPGENCGQCEHCRTGAYNLCSFSKFKGIPPVDGGMAEYITALATHVIPLPENLDSPTATLLEPFSVGLQAVDVA
DFRAGAKIAVLGGGPVGVLTAIAAKIRGCGDLWLTELYERRIEIARKLGIEKVINVAQEDPLKTIMEVTKKSGVDVVFET
TGNPKAVEQALQIVKRGGTVVFLGIGAGKVPINIDQVTRTGLKLLGSFRYQYHFAGAVALVKKHNLDFSPLVTNIYDFVD
IPKALEEVSTYKDRIMKGVIVFPQK
>Mature_345_residues
MKAAVLMEPKKIIFKEVPVPELEKGKVLIKVEAVGICGSDMHLYLDGHIGATVLDKPLVLGHEIVGTVIEVGEGVNRELL
GQRVIVDPGENCGQCEHCRTGAYNLCSFSKFKGIPPVDGGMAEYITALATHVIPLPENLDSPTATLLEPFSVGLQAVDVA
DFRAGAKIAVLGGGPVGVLTAIAAKIRGCGDLWLTELYERRIEIARKLGIEKVINVAQEDPLKTIMEVTKKSGVDVVFET
TGNPKAVEQALQIVKRGGTVVFLGIGAGKVPINIDQVTRTGLKLLGSFRYQYHFAGAVALVKKHNLDFSPLVTNIYDFVD
IPKALEEVSTYKDRIMKGVIVFPQK

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=330, Percent_Identity=31.2121212121212, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI4501929, Length=379, Percent_Identity=28.7598944591029, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4501933, Length=377, Percent_Identity=29.4429708222812, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788073, Length=341, Percent_Identity=36.950146627566, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1790045, Length=332, Percent_Identity=31.3253012048193, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1790718, Length=309, Percent_Identity=28.8025889967638, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI226510992, Length=343, Percent_Identity=27.9883381924198, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1787863, Length=347, Percent_Identity=25.3602305475504, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1788075, Length=314, Percent_Identity=26.4331210191083, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87081918, Length=291, Percent_Identity=31.6151202749141, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87082125, Length=296, Percent_Identity=29.7297297297297, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786825, Length=315, Percent_Identity=29.5238095238095, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788407, Length=325, Percent_Identity=24.6153846153846, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786552, Length=356, Percent_Identity=29.4943820224719, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082401, Length=130, Percent_Identity=36.9230769230769, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=338, Percent_Identity=35.7988165680473, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17562878, Length=331, Percent_Identity=35.6495468277946, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI71988145, Length=280, Percent_Identity=29.6428571428571, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17562584, Length=262, Percent_Identity=26.7175572519084, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71997431, Length=301, Percent_Identity=27.5747508305648, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25146526, Length=299, Percent_Identity=27.4247491638796, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17562582, Length=260, Percent_Identity=26.5384615384615, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323099, Length=350, Percent_Identity=33.7142857142857, Blast_Score=203, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6319955, Length=350, Percent_Identity=32.2857142857143, Blast_Score=203, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6322619, Length=350, Percent_Identity=32.2857142857143, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6319258, Length=319, Percent_Identity=31.974921630094, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319257, Length=331, Percent_Identity=29.9093655589124, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324486, Length=285, Percent_Identity=28.0701754385965, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323961, Length=293, Percent_Identity=27.3037542662116, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320033, Length=363, Percent_Identity=26.1707988980716, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323729, Length=274, Percent_Identity=27.007299270073, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323980, Length=147, Percent_Identity=36.734693877551, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319621, Length=275, Percent_Identity=26.9090909090909, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17737897, Length=326, Percent_Identity=32.5153374233129, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI17137530, Length=326, Percent_Identity=33.4355828220859, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI17737895, Length=296, Percent_Identity=29.0540540540541, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221457811, Length=301, Percent_Identity=23.9202657807309, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45551930, Length=315, Percent_Identity=23.4920634920635, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45550770, Length=315, Percent_Identity=23.4920634920635, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 37205; Mature: 37205

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAAVLMEPKKIIFKEVPVPELEKGKVLIKVEAVGICGSDMHLYLDGHIGATVLDKPLVL
CCCCEEECCHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCEEEEEECCCCCHHHCCCHHH
GHEIVGTVIEVGEGVNRELLGQRVIVDPGENCGQCEHCRTGAYNLCSFSKFKGIPPVDGG
HHHHHHHHHHHCCCCCHHHHCCEEEECCCCCCCCCHHHCCCCCCCCCHHHCCCCCCCCCC
MAEYITALATHVIPLPENLDSPTATLLEPFSVGLQAVDVADFRAGAKIAVLGGGPVGVLT
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEHHHHCCCCEEEEECCCHHHHHH
AIAAKIRGCGDLWLTELYERRIEIARKLGIEKVINVAQEDPLKTIMEVTKKSGVDVVFET
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEC
TGNPKAVEQALQIVKRGGTVVFLGIGAGKVPINIDQVTRTGLKLLGSFRYQYHFAGAVAL
CCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHEEEHHHHHEE
VKKHNLDFSPLVTNIYDFVDIPKALEEVSTYKDRIMKGVIVFPQK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
>Mature Secondary Structure
MKAAVLMEPKKIIFKEVPVPELEKGKVLIKVEAVGICGSDMHLYLDGHIGATVLDKPLVL
CCCCEEECCHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCEEEEEECCCCCHHHCCCHHH
GHEIVGTVIEVGEGVNRELLGQRVIVDPGENCGQCEHCRTGAYNLCSFSKFKGIPPVDGG
HHHHHHHHHHHCCCCCHHHHCCEEEECCCCCCCCCHHHCCCCCCCCCHHHCCCCCCCCCC
MAEYITALATHVIPLPENLDSPTATLLEPFSVGLQAVDVADFRAGAKIAVLGGGPVGVLT
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEHHHHCCCCEEEEECCCHHHHHH
AIAAKIRGCGDLWLTELYERRIEIARKLGIEKVINVAQEDPLKTIMEVTKKSGVDVVFET
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEC
TGNPKAVEQALQIVKRGGTVVFLGIGAGKVPINIDQVTRTGLKLLGSFRYQYHFAGAVAL
CCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHEEEHHHHHEE
VKKHNLDFSPLVTNIYDFVDIPKALEEVSTYKDRIMKGVIVFPQK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]