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The map label for this gene is murI

Identifier: 78043344

GI number: 78043344

Start: 268858

End: 269655

Strand: Direct

Name: murI

Synonym: CHY_0306

Alternate gene names: 78043344

Gene position: 268858-269655 (Clockwise)

Preceding gene: 78044861

Following gene: 78044463

Centisome position: 11.2

GC content: 41.23

Gene sequence:

>798_bases
TTGGACAAAGCATCATTGGGTATCGGAATTTTTGATTCGGGGGTTGGCGGAGTAACGGTATTAAAAGAACTGATAAATTT
GCTTCCCGGGGAAAACTTTTACTATTTGGGAGACACTGCGCATGTGCCTTACGGCTCAAAATCCAAAGAGGAATTATTAA
TTTTGGGCGATGCCATAATTCGCTTTTTTCTTAAGTTAGGGGTTAAGATGGTAGTTTTTGCCTGCAATACCAGTTCGGCC
ATAACCTTACCCATTTTAAAAGAGAGATACCCGGTTTTAATGGAAGGAATGCTTGAACCCTTAAGGAAAAATATGCCGGC
CAAAGCCAAGGTCGGGGTGCTGGCCACCGAGGCGACGGTGAAAAGTGGGCTTTATCAAAAAAAGCTTATGGAAACTAACA
AGTTTCAAGAAGTTTATATGATAGCTTGTCCCCAGTATGTACCCTTGATTGAAAAGGGGATTGTTTCGGGGAAAGAAGTA
GAAGAAGCTACCCGGGAATACTTTGAGCCACTTCTAAAAGAAGAAGTTAGAGATGTGGTCCTGGGTTGTACCCATTATCC
TTTTTTAACCGAAACTATTCAGAAACTTTACCCCAAAATTCAGGTAATTGACCCGGCGGCCTATGTTGCCCGGGAGGTTT
ATCGAAAATTAAAGGAAAACAAGCTTTTAGGTGAGAATGGCGGAAAAGTCCAGTTTTATGTGACCGGCGATGAAAAAAGT
CTAAAAAATCTTGGAAGCTTGTACTTAGGCTATAACATAGATAGGGTCCAAAAAATTGACTTGGAGGTAATAGTATGA

Upstream 100 bases:

>100_bases
GGTTTGTGTGGGATAATATTAAGAAAGGTACAGAGAAACGGTCTTGGCAGTAAAATATCTTGTTAAAAAACTTTAACCCA
TTTACAGGAGGTTTAGCATT

Downstream 100 bases:

>100_bases
AAGAGGTAAATTTAGTGCTCGGGGTGATTGGGGCTGATGTTCACGCCATTGGAAATAAAATTTTAGAGTATGCACTAACC
AATGCCGGTTTTAAGGTACA

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MDKASLGIGIFDSGVGGVTVLKELINLLPGENFYYLGDTAHVPYGSKSKEELLILGDAIIRFFLKLGVKMVVFACNTSSA
ITLPILKERYPVLMEGMLEPLRKNMPAKAKVGVLATEATVKSGLYQKKLMETNKFQEVYMIACPQYVPLIEKGIVSGKEV
EEATREYFEPLLKEEVRDVVLGCTHYPFLTETIQKLYPKIQVIDPAAYVAREVYRKLKENKLLGENGGKVQFYVTGDEKS
LKNLGSLYLGYNIDRVQKIDLEVIV

Sequences:

>Translated_265_residues
MDKASLGIGIFDSGVGGVTVLKELINLLPGENFYYLGDTAHVPYGSKSKEELLILGDAIIRFFLKLGVKMVVFACNTSSA
ITLPILKERYPVLMEGMLEPLRKNMPAKAKVGVLATEATVKSGLYQKKLMETNKFQEVYMIACPQYVPLIEKGIVSGKEV
EEATREYFEPLLKEEVRDVVLGCTHYPFLTETIQKLYPKIQVIDPAAYVAREVYRKLKENKLLGENGGKVQFYVTGDEKS
LKNLGSLYLGYNIDRVQKIDLEVIV
>Mature_265_residues
MDKASLGIGIFDSGVGGVTVLKELINLLPGENFYYLGDTAHVPYGSKSKEELLILGDAIIRFFLKLGVKMVVFACNTSSA
ITLPILKERYPVLMEGMLEPLRKNMPAKAKVGVLATEATVKSGLYQKKLMETNKFQEVYMIACPQYVPLIEKGIVSGKEV
EEATREYFEPLLKEEVRDVVLGCTHYPFLTETIQKLYPKIQVIDPAAYVAREVYRKLKENKLLGENGGKVQFYVTGDEKS
LKNLGSLYLGYNIDRVQKIDLEVIV

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=209, Percent_Identity=32.0574162679426, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_CARHZ (Q3AFA8)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359176.1
- HSSP:   P94556
- ProteinModelPortal:   Q3AFA8
- SMR:   Q3AFA8
- STRING:   Q3AFA8
- GeneID:   3728533
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0306
- NMPDR:   fig|246194.3.peg.353
- TIGR:   CHY_0306
- eggNOG:   COG0796
- HOGENOM:   HBG645102
- OMA:   AILGCTH
- BioCyc:   CHYD246194:CHY_0306-MONOMER
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 29668; Mature: 29668

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKASLGIGIFDSGVGGVTVLKELINLLPGENFYYLGDTAHVPYGSKSKEELLILGDAII
CCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEEHHHHH
RFFLKLGVKMVVFACNTSSAITLPILKERYPVLMEGMLEPLRKNMPAKAKVGVLATEATV
HHHHHHCCEEEEEEECCCCEEEEHHHHHCCHHHHHHHHHHHHHCCCCCHHEEEEEEHHHH
KSGLYQKKLMETNKFQEVYMIACPQYVPLIEKGIVSGKEVEEATREYFEPLLKEEVRDVV
HHHHHHHHHHHHCCHHEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGCTHYPFLTETIQKLYPKIQVIDPAAYVAREVYRKLKENKLLGENGGKVQFYVTGDEKS
HCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHH
LKNLGSLYLGYNIDRVQKIDLEVIV
HHHHHHHHHCCCCCHHEEECEEEEC
>Mature Secondary Structure
MDKASLGIGIFDSGVGGVTVLKELINLLPGENFYYLGDTAHVPYGSKSKEELLILGDAII
CCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEEHHHHH
RFFLKLGVKMVVFACNTSSAITLPILKERYPVLMEGMLEPLRKNMPAKAKVGVLATEATV
HHHHHHCCEEEEEEECCCCEEEEHHHHHCCHHHHHHHHHHHHHCCCCCHHEEEEEEHHHH
KSGLYQKKLMETNKFQEVYMIACPQYVPLIEKGIVSGKEVEEATREYFEPLLKEEVRDVV
HHHHHHHHHHHHCCHHEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGCTHYPFLTETIQKLYPKIQVIDPAAYVAREVYRKLKENKLLGENGGKVQFYVTGDEKS
HCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHH
LKNLGSLYLGYNIDRVQKIDLEVIV
HHHHHHHHHCCCCCHHEEECEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA