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The map label for this gene is paaK2 [H]

Identifier: 78043286

GI number: 78043286

Start: 641262

End: 642542

Strand: Direct

Name: paaK2 [H]

Synonym: CHY_0728

Alternate gene names: 78043286

Gene position: 641262-642542 (Clockwise)

Preceding gene: 78044212

Following gene: 78042649

Centisome position: 26.7

GC content: 46.68

Gene sequence:

>1281_bases
ATGTTTCGCAATCGGGAAGAAATTAAAGAAATACAGCTTCACCGCTTGAAAGACTTAGTGAAAAGGTTGTACGAACGGGT
GCCTTTTTACCGGCAGAAATTAGATGCCATTGGTTTTACACCGGACAAACTAAAATCCTTGGAAGATCTTCGGCATCTTC
CTTTTACTACCAAACAGGACCTGCGGGATAACTACCCCTTTGGCCTTTTTGCGGTAAGCCAGGAAGAAATTGTGAGAATT
CATGCTTCTTCCGGTACCACCGGCAAACCCACGGTGGTGGGTTATACCAGAAATGATATTGACCTCTGGAGTGAATTAAT
GGCAAGGTCAATGAGAAATGCCGGGGTAACTTCCCGCGATATCATTCACAATGCTTATGGCTACGGGCTTTTTACCGGGG
GGCTGGGTTTTCATTACGGCGCTGAAAAATTAGGGGCAACGGTGGTGCCGGTTTCCGGGGGTAACAGCAAACGGCAGTTA
ATGCTGATGGAAGATTTTGGAGCTACAGTCTTAACCTGTACCCCTTCTTATGCTTTGCATTTGGCCGAAGAAATGGAAGA
GCAGGGCATTTCCCGGGACAGGTTAAAATTGCGACTGGGGATTTTTGGTGCCGAGCCCTGGAGTGAAGAATTAAGGACCA
AAATTGAAGAAAAGTTGAAAATAAAAGCCATTGACGTTTACGGCTTAAGTGAAATCCTGGGACCGGGAGTAGCAATGGAG
TGTCAGGAACAAAAAGGCTTGCACATTAATGAAGACCATTTCCTTCCGGAAATCATTGACCCGGATACCGGTGAGGTATT
GCCGTACGGGGAAAAAGGGGAGCTGGTAATAACCACCCTGACCAAGGAAGGAATTCCCCTTTTAAGGTACCGCACCAGGG
ATATTACCGCCCTTTATCCGGAACCGTGTGCCTGCGGGAACGGCTTAATTAGAATGGACCGGGTAACCGGCAGAAGCGAT
GATATGTTGATTATTCGCGGGGTTAACGTCTTTCCTTCCCAAATTGAAACGGTGCTTTTATCTCTGGGTTATACCGAGCC
CCACTACATGATTGTGGTGGATAAAAAAGGAGCCTTGGACCAGTTAGAAGTTTGGGTGGAACTGTCGGAACAGTTCTTTT
CCGATGAAATAAAGAAGCTTGAACAATGGGAAAAGAAAGTGGAAGGAGAACTTTTCACGGTCCTGGGGATTAATGCTAAA
GTGCGGTTAGTGGAGCCCCGCAGTATTCCGCGCAGTGAAGGTAAGGCCAAGCGGATTTGGGATAGAAGAGAGTTAAAATA
A

Upstream 100 bases:

>100_bases
ACGGAAATTTTCTAAAAAAGTTTTAAGCTAAAAGGAAAAATTTTTGTTTTGTTGAATATATAAAAAAATTTTATCTATTT
GAAGGGGGGTAACCTGGAAA

Downstream 100 bases:

>100_bases
AAGGAGGGAGTTAGATGATTGTTAAGCAGCTGTCGGTTTTTTTGGAAAACAAATCGGGAAGACTTGCCCGGGTGACCGAG
ATTTTAGGGGAAAATAACAT

Product: phenylacetate-coenzyme A ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MFRNREEIKEIQLHRLKDLVKRLYERVPFYRQKLDAIGFTPDKLKSLEDLRHLPFTTKQDLRDNYPFGLFAVSQEEIVRI
HASSGTTGKPTVVGYTRNDIDLWSELMARSMRNAGVTSRDIIHNAYGYGLFTGGLGFHYGAEKLGATVVPVSGGNSKRQL
MLMEDFGATVLTCTPSYALHLAEEMEEQGISRDRLKLRLGIFGAEPWSEELRTKIEEKLKIKAIDVYGLSEILGPGVAME
CQEQKGLHINEDHFLPEIIDPDTGEVLPYGEKGELVITTLTKEGIPLLRYRTRDITALYPEPCACGNGLIRMDRVTGRSD
DMLIIRGVNVFPSQIETVLLSLGYTEPHYMIVVDKKGALDQLEVWVELSEQFFSDEIKKLEQWEKKVEGELFTVLGINAK
VRLVEPRSIPRSEGKAKRIWDRRELK

Sequences:

>Translated_426_residues
MFRNREEIKEIQLHRLKDLVKRLYERVPFYRQKLDAIGFTPDKLKSLEDLRHLPFTTKQDLRDNYPFGLFAVSQEEIVRI
HASSGTTGKPTVVGYTRNDIDLWSELMARSMRNAGVTSRDIIHNAYGYGLFTGGLGFHYGAEKLGATVVPVSGGNSKRQL
MLMEDFGATVLTCTPSYALHLAEEMEEQGISRDRLKLRLGIFGAEPWSEELRTKIEEKLKIKAIDVYGLSEILGPGVAME
CQEQKGLHINEDHFLPEIIDPDTGEVLPYGEKGELVITTLTKEGIPLLRYRTRDITALYPEPCACGNGLIRMDRVTGRSD
DMLIIRGVNVFPSQIETVLLSLGYTEPHYMIVVDKKGALDQLEVWVELSEQFFSDEIKKLEQWEKKVEGELFTVLGINAK
VRLVEPRSIPRSEGKAKRIWDRRELK
>Mature_426_residues
MFRNREEIKEIQLHRLKDLVKRLYERVPFYRQKLDAIGFTPDKLKSLEDLRHLPFTTKQDLRDNYPFGLFAVSQEEIVRI
HASSGTTGKPTVVGYTRNDIDLWSELMARSMRNAGVTSRDIIHNAYGYGLFTGGLGFHYGAEKLGATVVPVSGGNSKRQL
MLMEDFGATVLTCTPSYALHLAEEMEEQGISRDRLKLRLGIFGAEPWSEELRTKIEEKLKIKAIDVYGLSEILGPGVAME
CQEQKGLHINEDHFLPEIIDPDTGEVLPYGEKGELVITTLTKEGIPLLRYRTRDITALYPEPCACGNGLIRMDRVTGRSD
DMLIIRGVNVFPSQIETVLLSLGYTEPHYMIVVDKKGALDQLEVWVELSEQFFSDEIKKLEQWEKKVEGELFTVLGINAK
VRLVEPRSIPRSEGKAKRIWDRRELK

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=422, Percent_Identity=54.2654028436019, Blast_Score=460, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 48501; Mature: 48501

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRNREEIKEIQLHRLKDLVKRLYERVPFYRQKLDAIGFTPDKLKSLEDLRHLPFTTKQD
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHH
LRDNYPFGLFAVSQEEIVRIHASSGTTGKPTVVGYTRNDIDLWSELMARSMRNAGVTSRD
HHCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHH
IIHNAYGYGLFTGGLGFHYGAEKLGATVVPVSGGNSKRQLMLMEDFGATVLTCTPSYALH
HHHHHCCCEEEECCCCCCCCHHHHCCEEEEECCCCCCEEEEEEECCCCEEEEECCHHHHH
LAEEMEEQGISRDRLKLRLGIFGAEPWSEELRTKIEEKLKIKAIDVYGLSEILGPGVAME
HHHHHHHCCCCHHHEEEEEEEECCCCHHHHHHHHHHHHHEEEEEEEECHHHHHCCCCEEE
CQEQKGLHINEDHFLPEIIDPDTGEVLPYGEKGELVITTLTKEGIPLLRYRTRDITALYP
EHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCEEECC
EPCACGNGLIRMDRVTGRSDDMLIIRGVNVFPSQIETVLLSLGYTEPHYMIVVDKKGALD
CCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHH
QLEVWVELSEQFFSDEIKKLEQWEKKVEGELFTVLGINAKVRLVEPRSIPRSEGKAKRIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHH
DRRELK
HHHCCC
>Mature Secondary Structure
MFRNREEIKEIQLHRLKDLVKRLYERVPFYRQKLDAIGFTPDKLKSLEDLRHLPFTTKQD
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHH
LRDNYPFGLFAVSQEEIVRIHASSGTTGKPTVVGYTRNDIDLWSELMARSMRNAGVTSRD
HHCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHH
IIHNAYGYGLFTGGLGFHYGAEKLGATVVPVSGGNSKRQLMLMEDFGATVLTCTPSYALH
HHHHHCCCEEEECCCCCCCCHHHHCCEEEEECCCCCCEEEEEEECCCCEEEEECCHHHHH
LAEEMEEQGISRDRLKLRLGIFGAEPWSEELRTKIEEKLKIKAIDVYGLSEILGPGVAME
HHHHHHHCCCCHHHEEEEEEEECCCCHHHHHHHHHHHHHEEEEEEEECHHHHHCCCCEEE
CQEQKGLHINEDHFLPEIIDPDTGEVLPYGEKGELVITTLTKEGIPLLRYRTRDITALYP
EHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCEEECC
EPCACGNGLIRMDRVTGRSDDMLIIRGVNVFPSQIETVLLSLGYTEPHYMIVVDKKGALD
CCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHH
QLEVWVELSEQFFSDEIKKLEQWEKKVEGELFTVLGINAKVRLVEPRSIPRSEGKAKRIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHH
DRRELK
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]