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The map label for this gene is bioB

Identifier: 78042952

GI number: 78042952

Start: 787299

End: 788276

Strand: Direct

Name: bioB

Synonym: CHY_0889

Alternate gene names: 78042952

Gene position: 787299-788276 (Clockwise)

Preceding gene: 78043635

Following gene: 78043794

Centisome position: 32.78

GC content: 42.43

Gene sequence:

>978_bases
ATGTTTAATCGGATAGTAGCCTTAACCCAAAAAGTACTGGATGGCGGACAAATTAATCAAATTGAAGCGCTGGAGCTTGC
GCAGGCTGAGGGCGCAGATATTCTGATATTGGCAGCAATGGCGGCCAAGATCAGGGAAAAGTACGTGGGGGATAAGGTGG
AGTTATGTTCAATCATCAGTGTAAAAACCGGTCATTGTCCTGAGGACTGTGCCTTTTGCGCTCAATCGGTCCATCATCAT
ACAGAAATTACACCCACTGAAATGCTCGAGGAAGAAAAAATCTTGGCAAAAGCTAAGGCTATGGAAGCGGCCGGGGCCCA
TCGTTTTGATTTGGTAACGGCCGGTCTGGGAATGACCGAGGAGGACGAGGATTTTAAAAAAATTTTAGCTATTTATAAAC
GTTTGCGTCAGGAAGTAAAACTGGAATTATGCGCTTGCCTGGGGACATTAACCGAAAAAGCAGCTATGCAGCTGCGCGAA
GTCGGAGTTACTCGGTATAATCATAATCTGGAAACAGCGCGAAGTTTTTTTAGCAATATCGTTACAACTCATACTTATGA
TGAACGGATAGAAACCATTAAAAATGTTAAAAAAGCTGGAATGGAAGTATGTTGTGGTGGTATTATCGGTATGGGAGAAA
CTATGGAACAGCGAATTGAGTTTGCTTTTACGCTAAAAGAATTAGATGTTGATGCCATACCGATAAACGTGCTCAATCCA
ATTAAAGGAACAAAACTGGAAAACCGGCCGCTTTTATCGCCCCTGGAAGTTATCAAGACTTTTGCAATTTTCCGCTTCAT
TTTGCCGGATAAAAATATCAGATATGCCGGTGGTCGGGAGGTAAATTTGCGGGATATGCAGGCTCTGGGGTTAATGGCGG
GGTTAAACGGCATGTTAATCGGGCATTATTTAACAACTAAAGGCCGGGAAGTGGAAACAGATTTACAAATGATTAGAGAT
TTAGGTTTAAAAATTTAG

Upstream 100 bases:

>100_bases
TCTGGCGAACGGAGTTTTTGTTAACTATAAAATAAAAAACGTTGACAAATGTGAAATTCAACCGGTATTATAACTATAAA
CCGAGAGAGGGGGTAGAGCC

Downstream 100 bases:

>100_bases
AAGAGGGGGCCACTGGCCAAGGAAAGCGGTGGGAGATAAATGTAGCTGCGGAAAAAACGGTGGACGAAGGCTGAGCGTGA
AATTTCGATGAATTTCGTTA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MFNRIVALTQKVLDGGQINQIEALELAQAEGADILILAAMAAKIREKYVGDKVELCSIISVKTGHCPEDCAFCAQSVHHH
TEITPTEMLEEEKILAKAKAMEAAGAHRFDLVTAGLGMTEEDEDFKKILAIYKRLRQEVKLELCACLGTLTEKAAMQLRE
VGVTRYNHNLETARSFFSNIVTTHTYDERIETIKNVKKAGMEVCCGGIIGMGETMEQRIEFAFTLKELDVDAIPINVLNP
IKGTKLENRPLLSPLEVIKTFAIFRFILPDKNIRYAGGREVNLRDMQALGLMAGLNGMLIGHYLTTKGREVETDLQMIRD
LGLKI

Sequences:

>Translated_325_residues
MFNRIVALTQKVLDGGQINQIEALELAQAEGADILILAAMAAKIREKYVGDKVELCSIISVKTGHCPEDCAFCAQSVHHH
TEITPTEMLEEEKILAKAKAMEAAGAHRFDLVTAGLGMTEEDEDFKKILAIYKRLRQEVKLELCACLGTLTEKAAMQLRE
VGVTRYNHNLETARSFFSNIVTTHTYDERIETIKNVKKAGMEVCCGGIIGMGETMEQRIEFAFTLKELDVDAIPINVLNP
IKGTKLENRPLLSPLEVIKTFAIFRFILPDKNIRYAGGREVNLRDMQALGLMAGLNGMLIGHYLTTKGREVETDLQMIRD
LGLKI
>Mature_325_residues
MFNRIVALTQKVLDGGQINQIEALELAQAEGADILILAAMAAKIREKYVGDKVELCSIISVKTGHCPEDCAFCAQSVHHH
TEITPTEMLEEEKILAKAKAMEAAGAHRFDLVTAGLGMTEEDEDFKKILAIYKRLRQEVKLELCACLGTLTEKAAMQLRE
VGVTRYNHNLETARSFFSNIVTTHTYDERIETIKNVKKAGMEVCCGGIIGMGETMEQRIEFAFTLKELDVDAIPINVLNP
IKGTKLENRPLLSPLEVIKTFAIFRFILPDKNIRYAGGREVNLRDMQALGLMAGLNGMLIGHYLTTKGREVETDLQMIRD
LGLKI

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=285, Percent_Identity=37.5438596491228, Blast_Score=202, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6321725, Length=296, Percent_Identity=34.1216216216216, Blast_Score=180, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_CARHZ (Q3ADP5)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359739.1
- ProteinModelPortal:   Q3ADP5
- SMR:   Q3ADP5
- STRING:   Q3ADP5
- GeneID:   3726814
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_0889
- NMPDR:   fig|246194.3.peg.211
- TIGR:   CHY_0889
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK289638
- BioCyc:   CHYD246194:CHY_0889-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 36196; Mature: 36196

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNRIVALTQKVLDGGQINQIEALELAQAEGADILILAAMAAKIREKYVGDKVELCSIIS
CCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHC
VKTGHCPEDCAFCAQSVHHHTEITPTEMLEEEKILAKAKAMEAAGAHRFDLVTAGLGMTE
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
EDEDFKKILAIYKRLRQEVKLELCACLGTLTEKAAMQLREVGVTRYNHNLETARSFFSNI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VTTHTYDERIETIKNVKKAGMEVCCGGIIGMGETMEQRIEFAFTLKELDVDAIPINVLNP
HHCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCCEECCHHCCC
IKGTKLENRPLLSPLEVIKTFAIFRFILPDKNIRYAGGREVNLRDMQALGLMAGLNGMLI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHEE
GHYLTTKGREVETDLQMIRDLGLKI
HHHHCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFNRIVALTQKVLDGGQINQIEALELAQAEGADILILAAMAAKIREKYVGDKVELCSIIS
CCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHC
VKTGHCPEDCAFCAQSVHHHTEITPTEMLEEEKILAKAKAMEAAGAHRFDLVTAGLGMTE
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
EDEDFKKILAIYKRLRQEVKLELCACLGTLTEKAAMQLREVGVTRYNHNLETARSFFSNI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VTTHTYDERIETIKNVKKAGMEVCCGGIIGMGETMEQRIEFAFTLKELDVDAIPINVLNP
HHCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCCEECCHHCCC
IKGTKLENRPLLSPLEVIKTFAIFRFILPDKNIRYAGGREVNLRDMQALGLMAGLNGMLI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHEE
GHYLTTKGREVETDLQMIRDLGLKI
HHHHCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA