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The map label for this gene is yfnC [H]

Identifier: 78042682

GI number: 78042682

Start: 722409

End: 723653

Strand: Direct

Name: yfnC [H]

Synonym: CHY_0815

Alternate gene names: 78042682

Gene position: 722409-723653 (Clockwise)

Preceding gene: 78044871

Following gene: 78042959

Centisome position: 30.08

GC content: 45.54

Gene sequence:

>1245_bases
ATGGGGGAGGTTATACTGTTGGAAAAAGCGGTAAAATCGGCGAAACCAAATTTAGGCGTGCTTTTACTCTTATCTATGGG
ACATTTGGTTACCGATATGGCTCAGGGAGCTTTACCGGCTTTATTACCAATATTAAAACAGGCTTTTGCTTTAAACTATG
CTAAAACCAGTCTTTTAGTATTAATGTCAAATATAGCATCGTCCGTTATTCAACCTTTTTTTGGTTACATTGCCGATAGG
CGTCCCGCCCGGTGGTTATTGCCTTTAGGGTGTTTGCTGGCCGGAGTTGGTGTAGCGGTCACCGGCCGTATGCCCTGGTT
TTCTTTACTGTTAATTGTAGTTACAATTGCAAGCGTTGGTGTTGCGGCTTATCACCCTGAAGGCTCCCGCGTAGCCAACC
TGGCCAGCGGTGTGCGCAAGGGAAGGGGAATGGCTGTATTTTCGGTGGGTGGCAATTTAGGTTTTGGTTTGGGTTCGATA
TATATGGCCTGGCTTTTAAGCAAAGGGGGCTTAAAGGCAACGGTTTTCTTCCTTCTGCCAAGCCTTTTAATAGCGGTCCT
GTTTTTGTTTTCCTGGCCTCGCCTTACTTCTCTTCGGGACGAAGTGGTGGAGAAAAAAGCAAAAGGAAATGGTCAGGGAT
CCGGGCAGGAGATAAAGGCTGAAAGTAAAAAAAGAACCATTGCTGCTTTTTTCCTTCTTCTTTTATACATTGTTTTTCGT
ACCTGGATGCATGCGGGTCTTTATACGTACGTGCCATTGTATTATACCGATTATTTAAAAGGGGATCCCCATTTAGCAGG
TTACATTGTTTCGGTATTTTTACTTGCTGGTGCCGTTGGTACATTGCTGGGAGGACCACTTACGGATATTTTAGGGGCGC
CGAAGGTAATGGCCGGTTCTATGGCATTACTAATTCCGCTAATTTTCTTCTTTTCCCGTACTAACAACGGTTTTGTCTAC
TTTGTTTTACTTGCCTTGGTTGGGGCAACTTTAATCAGTACCTTTTCGACCACGGTGGTGCTCGGGCAGCAACTTATACC
CCACAGAAAAGGACTTGCCAGCGGCTTTACAATGGGTTTTGGTGTAGGCATGGGAGGAGTGGGAGTAACTTTACTTGGGG
TGCTTGCTGACCACCTGGGAGTGCCGGCGGTATTTCATGTTTTAAACATCCTGGCCGTAGGAGGATTTGTCCTGGCCTTA
ATACTTATACGGTATTTTCCCTGGAGTTCGAAAACTATGGAGTAG

Upstream 100 bases:

>100_bases
AGGAGGAATTATCTCTAAAGAAAAACCGGTGGAAAGTGGAGCAAAATAACTGGAAATGATTAAAAGCCTCCCGCAAAACC
GGGGGGCTTATTAACATCTA

Downstream 100 bases:

>100_bases
TTATTTATTTGAATGATTCGTAACAGCAGGAAGGTGAAGCCGTTAGATGAATTCTAATATCTTTGAGCTTGAATACCGAA
TTCCTTATTACGATGTAGAC

Product: putative fosmidomycin resistance protein

Products: fosmidomycin [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MGEVILLEKAVKSAKPNLGVLLLLSMGHLVTDMAQGALPALLPILKQAFALNYAKTSLLVLMSNIASSVIQPFFGYIADR
RPARWLLPLGCLLAGVGVAVTGRMPWFSLLLIVVTIASVGVAAYHPEGSRVANLASGVRKGRGMAVFSVGGNLGFGLGSI
YMAWLLSKGGLKATVFFLLPSLLIAVLFLFSWPRLTSLRDEVVEKKAKGNGQGSGQEIKAESKKRTIAAFFLLLLYIVFR
TWMHAGLYTYVPLYYTDYLKGDPHLAGYIVSVFLLAGAVGTLLGGPLTDILGAPKVMAGSMALLIPLIFFFSRTNNGFVY
FVLLALVGATLISTFSTTVVLGQQLIPHRKGLASGFTMGFGVGMGGVGVTLLGVLADHLGVPAVFHVLNILAVGGFVLAL
ILIRYFPWSSKTME

Sequences:

>Translated_414_residues
MGEVILLEKAVKSAKPNLGVLLLLSMGHLVTDMAQGALPALLPILKQAFALNYAKTSLLVLMSNIASSVIQPFFGYIADR
RPARWLLPLGCLLAGVGVAVTGRMPWFSLLLIVVTIASVGVAAYHPEGSRVANLASGVRKGRGMAVFSVGGNLGFGLGSI
YMAWLLSKGGLKATVFFLLPSLLIAVLFLFSWPRLTSLRDEVVEKKAKGNGQGSGQEIKAESKKRTIAAFFLLLLYIVFR
TWMHAGLYTYVPLYYTDYLKGDPHLAGYIVSVFLLAGAVGTLLGGPLTDILGAPKVMAGSMALLIPLIFFFSRTNNGFVY
FVLLALVGATLISTFSTTVVLGQQLIPHRKGLASGFTMGFGVGMGGVGVTLLGVLADHLGVPAVFHVLNILAVGGFVLAL
ILIRYFPWSSKTME
>Mature_413_residues
GEVILLEKAVKSAKPNLGVLLLLSMGHLVTDMAQGALPALLPILKQAFALNYAKTSLLVLMSNIASSVIQPFFGYIADRR
PARWLLPLGCLLAGVGVAVTGRMPWFSLLLIVVTIASVGVAAYHPEGSRVANLASGVRKGRGMAVFSVGGNLGFGLGSIY
MAWLLSKGGLKATVFFLLPSLLIAVLFLFSWPRLTSLRDEVVEKKAKGNGQGSGQEIKAESKKRTIAAFFLLLLYIVFRT
WMHAGLYTYVPLYYTDYLKGDPHLAGYIVSVFLLAGAVGTLLGGPLTDILGAPKVMAGSMALLIPLIFFFSRTNNGFVYF
VLLALVGATLISTFSTTVVLGQQLIPHRKGLASGFTMGFGVGMGGVGVTLLGVLADHLGVPAVFHVLNILAVGGFVLALI
LIRYFPWSSKTME

Specific function: Confers The Resistance Against Fosmidomycin. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786686, Length=386, Percent_Identity=30.5699481865285, Blast_Score=151, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44110; Mature: 43978

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVILLEKAVKSAKPNLGVLLLLSMGHLVTDMAQGALPALLPILKQAFALNYAKTSLLV
CCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LMSNIASSVIQPFFGYIADRRPARWLLPLGCLLAGVGVAVTGRMPWFSLLLIVVTIASVG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHC
VAAYHPEGSRVANLASGVRKGRGMAVFSVGGNLGFGLGSIYMAWLLSKGGLKATVFFLLP
HHEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
SLLIAVLFLFSWPRLTSLRDEVVEKKAKGNGQGSGQEIKAESKKRTIAAFFLLLLYIVFR
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TWMHAGLYTYVPLYYTDYLKGDPHLAGYIVSVFLLAGAVGTLLGGPLTDILGAPKVMAGS
HHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHH
MALLIPLIFFFSRTNNGFVYFVLLALVGATLISTFSTTVVLGQQLIPHRKGLASGFTMGF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
GVGMGGVGVTLLGVLADHLGVPAVFHVLNILAVGGFVLALILIRYFPWSSKTME
HCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GEVILLEKAVKSAKPNLGVLLLLSMGHLVTDMAQGALPALLPILKQAFALNYAKTSLLV
CCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LMSNIASSVIQPFFGYIADRRPARWLLPLGCLLAGVGVAVTGRMPWFSLLLIVVTIASVG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHC
VAAYHPEGSRVANLASGVRKGRGMAVFSVGGNLGFGLGSIYMAWLLSKGGLKATVFFLLP
HHEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
SLLIAVLFLFSWPRLTSLRDEVVEKKAKGNGQGSGQEIKAESKKRTIAAFFLLLLYIVFR
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TWMHAGLYTYVPLYYTDYLKGDPHLAGYIVSVFLLAGAVGTLLGGPLTDILGAPKVMAGS
HHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHH
MALLIPLIFFFSRTNNGFVYFVLLALVGATLISTFSTTVVLGQQLIPHRKGLASGFTMGF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
GVGMGGVGVTLLGVLADHLGVPAVFHVLNILAVGGFVLALILIRYFPWSSKTME
HCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: fosmidomycin [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: fosmidomycin [Cytoplasm] + Proton [Periplasm] = fosmidomycin [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]