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The map label for this gene is proB [H]

Identifier: 77761174

GI number: 77761174

Start: 2272247

End: 2273401

Strand: Direct

Name: proB [H]

Synonym: XC_1879

Alternate gene names: 77761174

Gene position: 2272247-2273401 (Clockwise)

Preceding gene: 66768199

Following gene: 66768201

Centisome position: 44.13

GC content: 69.87

Gene sequence:

>1155_bases
ATGACCCAGCCGGTCGTCGCGACCTCGCTGTTTACCGAGCAGGTGCTGCCGCCGTGGCGGCGCGCAGTGCTCAAGGTCGG
CAGCAGCCTGCTGGCGGCCGATGGCGGCGGCCTGTCGCCGCGGTTTGCGCTGGGCCTGGCGCAGTTTGTATCGGCCAATC
TGGCGGCGGGGCGCGAAGTGGTGATCGTCTCGTCCGGCGCGGTGGCGGCCGGGCGCGCCATCCTGCCCAAGGCAGCCGAA
GCTGGCGCCGCCATCGCTGCGCGCCAGGCACTGGCCGCGCTGGGCCAGGCGCAGTTGATCGCGTTGTGGCAGCGCTTTTT
CGAGCGCCCGGTGGCGCAGGTGCTGCTCACCCACGACGACCTGCGCAACCGCCGCCGCTACCTCAATGCGCGCGCCACGC
TGGGCGAACTGTTGCGGCTGGGCGCATTGCCGGTGATCAACGAGAACGACACCGTGTCGGTGGATGAACTCAAGCTCGGC
GACAACGACAACCTGGCCGCGATCGTGGCCGCGCTGGTCGATGCCGATGCCTTGTTCATCGCCACCGATATCGATGGCCT
GTACAGCGCCGACCCGCGCAGCAACCCGCTGGCACGCCCGCTCGACGAGGTGGCCGAACTCAGCGCCGAGGTGCTGGCGA
TGGCCGGCGGCAGCGGCAGCAGCGTGGGCACCGGCGGCATGCGCACCAAGCTGGAAGCCGCCGCCAAGGCCGGTGCGGCC
GGGATCGAAACCTATCTGTTCAACGGCCGCAGTGCCGAGGTGGTGCGCGGGCTGGCGCAGGACCGGCTGTGCGGCACGCG
CATCCACGCCGCGCGCACGCGCATTGCCGCGCGCAAATACTGGCTGCGGCATGCGCCGGTGGAGCCGGGCACGATCCTGA
TCGACGCCGGCGCCGCGCTGGCGCTCACCGACAAGGGCGCCTCGCTGCTACCCGGTGGCGTGGCCGGTGCCGAAGGCGAT
TTCCGCCGTGGCGACATGGTGGAAATCCACCTGCGCGACAGCGTGGGCAGCCGCTGCCTGGCACGTGGCGTGAGCCAGTA
TTCGGCACTTGATGTGCGCCGCATCGCCCGCCGCCATTCGCGCGAGATCGAACCGATCCTCGGCTACAGCTACGGCGAGA
ATGTGGTGCATCGTGATGATCTGGTAGTTTTGTAG

Upstream 100 bases:

>100_bases
GGTGCCTCCACGCTGACGGTGTACGAATGGAACGCGCAGTGAGGCTGCACATCCGCGTGGCACACGTGGTGATCGCTGCC
GCAATCACTAGGGCACGGCC

Downstream 100 bases:

>100_bases
CCGCGAATCGGGAATCGGGACTTGGGAATCGCAACAGCGCTTACACCCGTGGTGTTTCCGATGCTCAGACGTGAACGACG
CAGTGATGCAGGCCGTTGCC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MTQPVVATSLFTEQVLPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGREVVIVSSGAVAAGRAILPKAAE
AGAAIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDDLRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLG
DNDNLAAIVAALVDADALFIATDIDGLYSADPRSNPLARPLDEVAELSAEVLAMAGGSGSSVGTGGMRTKLEAAAKAGAA
GIETYLFNGRSAEVVRGLAQDRLCGTRIHAARTRIAARKYWLRHAPVEPGTILIDAGAALALTDKGASLLPGGVAGAEGD
FRRGDMVEIHLRDSVGSRCLARGVSQYSALDVRRIARRHSREIEPILGYSYGENVVHRDDLVVL

Sequences:

>Translated_384_residues
MTQPVVATSLFTEQVLPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGREVVIVSSGAVAAGRAILPKAAE
AGAAIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDDLRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLG
DNDNLAAIVAALVDADALFIATDIDGLYSADPRSNPLARPLDEVAELSAEVLAMAGGSGSSVGTGGMRTKLEAAAKAGAA
GIETYLFNGRSAEVVRGLAQDRLCGTRIHAARTRIAARKYWLRHAPVEPGTILIDAGAALALTDKGASLLPGGVAGAEGD
FRRGDMVEIHLRDSVGSRCLARGVSQYSALDVRRIARRHSREIEPILGYSYGENVVHRDDLVVL
>Mature_383_residues
TQPVVATSLFTEQVLPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGREVVIVSSGAVAAGRAILPKAAEA
GAAIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDDLRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLGD
NDNLAAIVAALVDADALFIATDIDGLYSADPRSNPLARPLDEVAELSAEVLAMAGGSGSSVGTGGMRTKLEAAAKAGAAG
IETYLFNGRSAEVVRGLAQDRLCGTRIHAARTRIAARKYWLRHAPVEPGTILIDAGAALALTDKGASLLPGGVAGAEGDF
RRGDMVEIHLRDSVGSRCLARGVSQYSALDVRRIARRHSREIEPILGYSYGENVVHRDDLVVL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=282, Percent_Identity=29.0780141843972, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI62912457, Length=280, Percent_Identity=28.9285714285714, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786437, Length=364, Percent_Identity=44.5054945054945, Blast_Score=268, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI17569969, Length=268, Percent_Identity=32.8358208955224, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17569967, Length=268, Percent_Identity=32.8358208955224, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320506, Length=413, Percent_Identity=35.1089588377724, Blast_Score=219, Evalue=6e-58,
Organism=Saccharomyces cerevisiae, GI6321822, Length=412, Percent_Identity=34.9514563106796, Blast_Score=213, Evalue=4e-56,
Organism=Drosophila melanogaster, GI21357643, Length=309, Percent_Identity=32.6860841423948, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40441; Mature: 40310

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPVVATSLFTEQVLPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGREV
CCCCHHHHHHHHHHCCCHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEE
VIVSSGAVAAGRAILPKAAEAGAAIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDD
EEEECCCHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLGDNDNLAAIVAALVDADALFI
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEHEEEEECCCCCHHHHHHHHHCCCEEEE
ATDIDGLYSADPRSNPLARPLDEVAELSAEVLAMAGGSGSSVGTGGMRTKLEAAAKAGAA
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHCCCC
GIETYLFNGRSAEVVRGLAQDRLCGTRIHAARTRIAARKYWLRHAPVEPGTILIDAGAAL
CCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEE
ALTDKGASLLPGGVAGAEGDFRRGDMVEIHLRDSVGSRCLARGVSQYSALDVRRIARRHS
EEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHC
REIEPILGYSYGENVVHRDDLVVL
CCCCHHCCCCCCCCCEECCCEEEC
>Mature Secondary Structure 
TQPVVATSLFTEQVLPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGREV
CCCHHHHHHHHHHCCCHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEE
VIVSSGAVAAGRAILPKAAEAGAAIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDD
EEEECCCHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLGDNDNLAAIVAALVDADALFI
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEHEEEEECCCCCHHHHHHHHHCCCEEEE
ATDIDGLYSADPRSNPLARPLDEVAELSAEVLAMAGGSGSSVGTGGMRTKLEAAAKAGAA
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHCCCC
GIETYLFNGRSAEVVRGLAQDRLCGTRIHAARTRIAARKYWLRHAPVEPGTILIDAGAAL
CCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEE
ALTDKGASLLPGGVAGAEGDFRRGDMVEIHLRDSVGSRCLARGVSQYSALDVRRIARRHS
EEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHC
REIEPILGYSYGENVVHRDDLVVL
CCCCHHCCCCCCCCCEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]