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The map label for this gene is rlmL

Identifier: 77747722

GI number: 77747722

Start: 2380069

End: 2382243

Strand: Reverse

Name: rlmL

Synonym: PD2023

Alternate gene names: 77747722

Gene position: 2382243-2380069 (Counterclockwise)

Preceding gene: 28199885

Following gene: 28199881

Centisome position: 94.54

GC content: 58.21

Gene sequence:

>2175_bases
GTGAGATTTTTTGTTTCCTGCGCTAAAGGTTTGGAATATCTGTTGGTTGATGAAGTGTTGGCGCTTGGTGCCGCTGGGGC
TACGGCGACAGTGGCCGGTGTCAATGTGGAAGGTGGGTTGTGCGATGCGCAGCGTCTGGTGCTGTGGTCGCGTTTGGCCA
GCCGTGTGTTGTGGCCGTTGGCGGCGTTCGCATGTGCGGATGAGGATGCGTTATATGCGGGTGTTGCGGCGTTGCCGTGG
GTGGAGCATGTTTTGCCGGGCCAGACGTTGGCGGTGGATGCGCACGTTTCCGGGGAGGCGATCACTCATGCGCGTTACGC
TGCACAGCGTGTGAAGGATGCGGTGGTCGATACGTTGCGTGATGCTGGTGTGGTGCGGCCATCGGTTGATGTGGAGCACC
CAGATGTACGTTTGAATTTGTCGCTCCGCAAGGGGCGTGCGACGTTGTCGGTGGATCTGGGTGGTCGGGCGCTGCATCAC
CGTGGTTGGCGTCAGGCACCGCATGCGGCGTCATTGAAGGAGCATTTGGCGGCTGCGGTGTTGTTGCGTGCCGGCTGGGC
CAAGGTCTATGCCGAAGGTGGGGGGCTGCTCGATCCGATGTGTGGCAGTGGCACGCTCTTGATTGAGGGGGCGTTGATGG
TGGCCGATGTGGCACCTGGGTTGTCGCGTTATGCCGATCCTGATGCGATGTCTCATGTGTCGGTAGCAGAGCGTCCGGTG
TTGTTGCCGAGTCGTTGGCGTGGTTTTGATGTCGTGGCGTGGGAAGCCTTAGTGGTTGATGCGCAGCAGCGTGCCAGGCG
AGGGTTGGCGGAGTTGCGTCCGGTGTTGCACGGCAGTGACATTGATCCTCGGGCACTTGGTGCTGCGTTTGCGAATGCGC
GGGCGGCGGGTGTTCAAGATGCGATTGAGTTTGTTGTTGCCGGTATTGATGTGCTGCCGGCGGTGTCTGAGCCTCACGGT
GTGGTGGTGTGTAACGCGCCCTATGATGTGCGTTTGGCGGCCGATCCGGGGTTGTATCGGCATTTGGGGGATGCGTTACG
GCGTGTGGTCCCGCGCTGGCGGGCCGCGCTAGTGTGTGGCAGTAGTACGTTGGCATTTGCAACGGGGTTGCGGGCTGATA
AGAAGTATCAGTTTTTCAACGGTGCGTTGGAATGTGTGTTGATTGTCTGTGATCCGGTGGTGCCGTTAGCGCGTGAGGCA
GGGGGAGCACAGGCGTTGAGTGAGGGGGCGCAGATGGCTGCTAATCGTCTGCGCAAGAATGTGCAGCGCTTGAAGAAGTG
GCGTATCCGGGCGGGGGTGGAGTGTTACCGGGTTTACGATGCTGATTTGCCGGAGTATGCGGCTGCGATTGATGTGTATC
AGGAGGTGGATGGTGCGCGGCGGTTATTCCTGCATGTGCAGGAGTATGCGGCGCCGGCGTCGATTCCTGAGGGGGATGTG
CGGCGGAGGCGTCATGAGTTGCTTGCTGCGGTACGCGCGGTGTTTGATGTGTCGGTGGCTCAGGTGGCTTTAAAAACGCG
GCAGCGCGGTAAGGGGGGGAGTCAGTACGGTTGTTTTGCGCAGCGTGGGGAGTTTTTTCATGTGTGTGAGCATGGTGCGT
TGTTGCGTGTGAATTTGTTTGATTATCTGGATACGGGGTTGTTTCTTGATCATCGTCCTTTGCGCGGGCGCATGGCGCGT
GAGGCGGTGGGTAAGCGTTTTCTAAATGTGTTCTGTTACACGGGTGTGGCGAGTGTGGAGGCAGCAGTGGCTGGTGCTGC
GGCGACAACCAGTGTGGATTTGTCATCCACGTATTTGCATTGGTGTACGGATAATTTTGCGTTGAATGGGCAAGGTGGGG
TGAGGCATCGTTTGGTGCAGGCGGATGCGTTGGCTTGGTTGGAGGCGGAGCGTGGTCAATACGATGTCATTTTTTGTGAT
CCGCCGACGTTTTCGAATTCGGCGCGCGCTGATGATTTTGATGTGCAACGCGATCATGTGCGTTTGCTGCGTGCGGCTGT
TGCGCGGTTGACCCCTGGTGGGGTGCTTTACTTTTCCAATAATTTCCGCCGTTTCCGTTTGGACGTGGATGCGGTTGCTG
CGTTTGCGCAGTGTGAGGAAATCAGCCCGGTGACGATTGATTTAGATTTTTCGCGCAATACGAGGATCCATCGTACGTGG
CTGTTGTGGCGGTAG

Upstream 100 bases:

>100_bases
GGCGTGATGGCCCAACACGGCGCTCCGTGTGGTGGCTTCTGTTGCTGTTTTCCCCATCGTGGCAGGTGTGAGGTGCTGGT
TTCCGGTTTGGGTTGATGAT

Downstream 100 bases:

>100_bases
TGGGGTGTTTGCAGGGAGTTGTTGGGGTGTTTCTCTGGAGGTAATCGGCAGTGTTGCGCTGTACGGTTGCGGTGGTAGGG
CCGTTAGGGGCAATGGTGGG

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 724; Mature: 724

Protein sequence:

>724_residues
MRFFVSCAKGLEYLLVDEVLALGAAGATATVAGVNVEGGLCDAQRLVLWSRLASRVLWPLAAFACADEDALYAGVAALPW
VEHVLPGQTLAVDAHVSGEAITHARYAAQRVKDAVVDTLRDAGVVRPSVDVEHPDVRLNLSLRKGRATLSVDLGGRALHH
RGWRQAPHAASLKEHLAAAVLLRAGWAKVYAEGGGLLDPMCGSGTLLIEGALMVADVAPGLSRYADPDAMSHVSVAERPV
LLPSRWRGFDVVAWEALVVDAQQRARRGLAELRPVLHGSDIDPRALGAAFANARAAGVQDAIEFVVAGIDVLPAVSEPHG
VVVCNAPYDVRLAADPGLYRHLGDALRRVVPRWRAALVCGSSTLAFATGLRADKKYQFFNGALECVLIVCDPVVPLAREA
GGAQALSEGAQMAANRLRKNVQRLKKWRIRAGVECYRVYDADLPEYAAAIDVYQEVDGARRLFLHVQEYAAPASIPEGDV
RRRRHELLAAVRAVFDVSVAQVALKTRQRGKGGSQYGCFAQRGEFFHVCEHGALLRVNLFDYLDTGLFLDHRPLRGRMAR
EAVGKRFLNVFCYTGVASVEAAVAGAAATTSVDLSSTYLHWCTDNFALNGQGGVRHRLVQADALAWLEAERGQYDVIFCD
PPTFSNSARADDFDVQRDHVRLLRAAVARLTPGGVLYFSNNFRRFRLDVDAVAAFAQCEEISPVTIDLDFSRNTRIHRTW
LLWR

Sequences:

>Translated_724_residues
MRFFVSCAKGLEYLLVDEVLALGAAGATATVAGVNVEGGLCDAQRLVLWSRLASRVLWPLAAFACADEDALYAGVAALPW
VEHVLPGQTLAVDAHVSGEAITHARYAAQRVKDAVVDTLRDAGVVRPSVDVEHPDVRLNLSLRKGRATLSVDLGGRALHH
RGWRQAPHAASLKEHLAAAVLLRAGWAKVYAEGGGLLDPMCGSGTLLIEGALMVADVAPGLSRYADPDAMSHVSVAERPV
LLPSRWRGFDVVAWEALVVDAQQRARRGLAELRPVLHGSDIDPRALGAAFANARAAGVQDAIEFVVAGIDVLPAVSEPHG
VVVCNAPYDVRLAADPGLYRHLGDALRRVVPRWRAALVCGSSTLAFATGLRADKKYQFFNGALECVLIVCDPVVPLAREA
GGAQALSEGAQMAANRLRKNVQRLKKWRIRAGVECYRVYDADLPEYAAAIDVYQEVDGARRLFLHVQEYAAPASIPEGDV
RRRRHELLAAVRAVFDVSVAQVALKTRQRGKGGSQYGCFAQRGEFFHVCEHGALLRVNLFDYLDTGLFLDHRPLRGRMAR
EAVGKRFLNVFCYTGVASVEAAVAGAAATTSVDLSSTYLHWCTDNFALNGQGGVRHRLVQADALAWLEAERGQYDVIFCD
PPTFSNSARADDFDVQRDHVRLLRAAVARLTPGGVLYFSNNFRRFRLDVDAVAAFAQCEEISPVTIDLDFSRNTRIHRTW
LLWR
>Mature_724_residues
MRFFVSCAKGLEYLLVDEVLALGAAGATATVAGVNVEGGLCDAQRLVLWSRLASRVLWPLAAFACADEDALYAGVAALPW
VEHVLPGQTLAVDAHVSGEAITHARYAAQRVKDAVVDTLRDAGVVRPSVDVEHPDVRLNLSLRKGRATLSVDLGGRALHH
RGWRQAPHAASLKEHLAAAVLLRAGWAKVYAEGGGLLDPMCGSGTLLIEGALMVADVAPGLSRYADPDAMSHVSVAERPV
LLPSRWRGFDVVAWEALVVDAQQRARRGLAELRPVLHGSDIDPRALGAAFANARAAGVQDAIEFVVAGIDVLPAVSEPHG
VVVCNAPYDVRLAADPGLYRHLGDALRRVVPRWRAALVCGSSTLAFATGLRADKKYQFFNGALECVLIVCDPVVPLAREA
GGAQALSEGAQMAANRLRKNVQRLKKWRIRAGVECYRVYDADLPEYAAAIDVYQEVDGARRLFLHVQEYAAPASIPEGDV
RRRRHELLAAVRAVFDVSVAQVALKTRQRGKGGSQYGCFAQRGEFFHVCEHGALLRVNLFDYLDTGLFLDHRPLRGRMAR
EAVGKRFLNVFCYTGVASVEAAVAGAAATTSVDLSSTYLHWCTDNFALNGQGGVRHRLVQADALAWLEAERGQYDVIFCD
PPTFSNSARADDFDVQRDHVRLLRAAVARLTPGGVLYFSNNFRRFRLDVDAVAAFAQCEEISPVTIDLDFSRNTRIHRTW
LLWR

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=737, Percent_Identity=42.3337856173677, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI145693111, Length=152, Percent_Identity=32.2368421052632, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_XYLF2 (B2IA87)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830797.1
- ProteinModelPortal:   B2IA87
- GeneID:   6203194
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_2125
- HOGENOM:   HBG289536
- ProtClustDB:   PRK11783
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 78820; Mature: 78820

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS51165 THUMP; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFFVSCAKGLEYLLVDEVLALGAAGATATVAGVNVEGGLCDAQRLVLWSRLASRVLWPL
CCEEHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
AAFACADEDALYAGVAALPWVEHVLPGQTLAVDAHVSGEAITHARYAAQRVKDAVVDTLR
HHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
DAGVVRPSVDVEHPDVRLNLSLRKGRATLSVDLGGRALHHRGWRQAPHAASLKEHLAAAV
HCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHH
LLRAGWAKVYAEGGGLLDPMCGSGTLLIEGALMVADVAPGLSRYADPDAMSHVSVAERPV
HHHHCHHHEEECCCCCCCCCCCCCEEEEECCEEEEHHCCCHHHCCCCCHHHHHHHHCCCC
LLPSRWRGFDVVAWEALVVDAQQRARRGLAELRPVLHGSDIDPRALGAAFANARAAGVQD
CCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCHHCCHHH
AIEFVVAGIDVLPAVSEPHGVVVCNAPYDVRLAADPGLYRHLGDALRRVVPRWRAALVCG
HHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEC
SSTLAFATGLRADKKYQFFNGALECVLIVCDPVVPLAREAGGAQALSEGAQMAANRLRKN
CCHHHHHHCCCCCCCHHHHHHHEEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
VQRLKKWRIRAGVECYRVYDADLPEYAAAIDVYQEVDGARRLFLHVQEYAAPASIPEGDV
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
RRRRHELLAAVRAVFDVSVAQVALKTRQRGKGGSQYGCFAQRGEFFHVCEHGALLRVNLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEECCCCEEEEEHH
DYLDTGLFLDHRPLRGRMAREAVGKRFLNVFCYTGVASVEAAVAGAAATTSVDLSSTYLH
HHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHCCEEECCCCEEE
WCTDNFALNGQGGVRHRLVQADALAWLEAERGQYDVIFCDPPTFSNSARADDFDVQRDHV
EECCCEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHH
RLLRAAVARLTPGGVLYFSNNFRRFRLDVDAVAAFAQCEEISPVTIDLDFSRNTRIHRTW
HHHHHHHHHCCCCCEEEEECCCEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEE
LLWR
EEEC
>Mature Secondary Structure
MRFFVSCAKGLEYLLVDEVLALGAAGATATVAGVNVEGGLCDAQRLVLWSRLASRVLWPL
CCEEHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
AAFACADEDALYAGVAALPWVEHVLPGQTLAVDAHVSGEAITHARYAAQRVKDAVVDTLR
HHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
DAGVVRPSVDVEHPDVRLNLSLRKGRATLSVDLGGRALHHRGWRQAPHAASLKEHLAAAV
HCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHH
LLRAGWAKVYAEGGGLLDPMCGSGTLLIEGALMVADVAPGLSRYADPDAMSHVSVAERPV
HHHHCHHHEEECCCCCCCCCCCCCEEEEECCEEEEHHCCCHHHCCCCCHHHHHHHHCCCC
LLPSRWRGFDVVAWEALVVDAQQRARRGLAELRPVLHGSDIDPRALGAAFANARAAGVQD
CCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCHHCCHHH
AIEFVVAGIDVLPAVSEPHGVVVCNAPYDVRLAADPGLYRHLGDALRRVVPRWRAALVCG
HHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEC
SSTLAFATGLRADKKYQFFNGALECVLIVCDPVVPLAREAGGAQALSEGAQMAANRLRKN
CCHHHHHHCCCCCCCHHHHHHHEEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
VQRLKKWRIRAGVECYRVYDADLPEYAAAIDVYQEVDGARRLFLHVQEYAAPASIPEGDV
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
RRRRHELLAAVRAVFDVSVAQVALKTRQRGKGGSQYGCFAQRGEFFHVCEHGALLRVNLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEECCCCEEEEEHH
DYLDTGLFLDHRPLRGRMAREAVGKRFLNVFCYTGVASVEAAVAGAAATTSVDLSSTYLH
HHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHCCEEECCCCEEE
WCTDNFALNGQGGVRHRLVQADALAWLEAERGQYDVIFCDPPTFSNSARADDFDVQRDHV
EECCCEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHH
RLLRAAVARLTPGGVLYFSNNFRRFRLDVDAVAAFAQCEEISPVTIDLDFSRNTRIHRTW
HHHHHHHHHCCCCCEEEEECCCEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEE
LLWR
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA