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The map label for this gene is pglA [H]

Identifier: 77747698

GI number: 77747698

Start: 1727481

End: 1729088

Strand: Reverse

Name: pglA [H]

Synonym: PD1485

Alternate gene names: 77747698

Gene position: 1729088-1727481 (Counterclockwise)

Preceding gene: 28199367

Following gene: 28199355

Centisome position: 68.62

GC content: 52.92

Gene sequence:

>1608_bases
GTGTTTGGACTGCACTGTTTTGCCGCAATGGGCGGCACTGCTGATGTCTTTCGAGTGTCACAGCTGTCCTCATCAGATCT
TTCCGAGCCGGTATCGGTGAAAACGCTATGGGGCGAGGTGCAACGTCCCAGCCTACCTACCCACGTGTGTACGGTATTGC
CTGCGCGCCTGACTCCTAAACATGGCTCCATTGACGCACTCGATGCCAATCCAAAGGTTTCCAAGCCTGATACCAAGCGT
TTACAGGATGCCATTGATGATTGTCCCGCCGGTAGCGCGGTCAAACTAGTGATCGACAGTCATCGCAAATCCGGCTTTTT
GAGTGGGCCGTTGCACTTGAAATCTGGGGTGACGTTGTGGATTGACGAGGGTGTTACGCTGTTTGCATCGCGCAACCCCA
AGGATTACGACAAAGGCAATGGTACGTGCGGTACTGCAACGTCAACTCATGAGTTCAGTTGTATGCCTTTGATCTCCGCG
ATCAACACCACCGGTAGCGGTATCGTGGGTGGGGGTGTCATTGATGGGCGTGGCGGTAGCATTTTGACTGGCGGTAAGTA
TGCTCGGCAGCGTACCTGGTGGGACCTGGCCTATCAGAACAAGCGCCATGCACTGCATCAACAGGTACCACGGTTGATCC
AAATCAGGGGCGGTAACGACTTCACGTTGTACCGTGTTGCCATCGAAAATGCACCTAACTTCCATGTGGTTGCTGATACG
GTTAGCGGTGTCACCGCATGGGGCATCCGCATCCTGACCCCAAGCTTGGTCTATACCACGCCGGGGTACCACTGTCCGCC
CGGTACCACGCCGGATGTTGTGACGCCGGCGACATGCTTTACCCCGGACACGGTCAAGAACACCGATGGCTTTGATCCTG
GGCAATCAAACCACGTGCTGCTAGCGTACTCCTACATCAGCACTGGGGATGACCACGTTGCCATCAAAGCGCGGGGCAAG
ATGCCAAGCTACGCTCTGAGCTTCTTACACAACCACTTTGGCTATGGACATGGCATGTCCATCGGTAGCGACACCGAGTC
AGGTGTACACGATATGGAAGTCAGTGATTTGAGTATCGACGGCTTTGACAGCCCTAACAGCAATGGTTTGCAGATGAAAT
CTGATGCCGATCATGGCGGAGTGGTCGACCATGTCACCTACTCCAAAATATGCATGCGTCGTCTGAAACGTCCGCTGGCG
TTTGACACCTTCTACAAACCTTCCAACGGGAATTCTTATCCACTCTTCAAAAATATCGTGCTGCAAGATATTCATGTGCT
TGAAAGTCCTGTCTTTGGTGCTGGTCAGCTGCTTTTCATGGGGATCCTGGGCAGTGGGAATAATCTGCCAATGACGCTGA
GCATGGATAACGTCGTATTCGACGGTTTTTTGCCAACATTGATAGCGCCTCCCAGCAGTGTCGTATTCGCCAATCCGCAG
GCAGTGCATTTTCACTTCGGTCCCGGTCCGGTCAGTTTCGCTCCTTTGATTACCCCCTCGGTTGCTTACGATGTGACTGT
GAGTGGAAGTCCAGGTGTGGGCAACCCATACGATTGTTCGGCGGCATTTATCAATTTTTCCAGCGTATTTCCTGATTCGC
CTATCTAA

Upstream 100 bases:

>100_bases
GGCCTAACGATGTTGGTCCTTTTTGCCTCCGGTGCGACTGCTTCCTTACCCTTTTTTTCAGAGTGTTTTTCTTATGAACC
TTGACCGTTTCCTCCCCCTA

Downstream 100 bases:

>100_bases
TCAATGTGTGTCCAATCGCAGCTGTACAGCGGCCATGCGGATGTTGAAACAGCAGTGGCCGGACGTTGATCCAAGCAACA
CAGCTGCTGTCGGTCACAAT

Product: polygalacturonase precursor

Products: NA

Alternate protein names: PGA; Pectinase [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MFGLHCFAAMGGTADVFRVSQLSSSDLSEPVSVKTLWGEVQRPSLPTHVCTVLPARLTPKHGSIDALDANPKVSKPDTKR
LQDAIDDCPAGSAVKLVIDSHRKSGFLSGPLHLKSGVTLWIDEGVTLFASRNPKDYDKGNGTCGTATSTHEFSCMPLISA
INTTGSGIVGGGVIDGRGGSILTGGKYARQRTWWDLAYQNKRHALHQQVPRLIQIRGGNDFTLYRVAIENAPNFHVVADT
VSGVTAWGIRILTPSLVYTTPGYHCPPGTTPDVVTPATCFTPDTVKNTDGFDPGQSNHVLLAYSYISTGDDHVAIKARGK
MPSYALSFLHNHFGYGHGMSIGSDTESGVHDMEVSDLSIDGFDSPNSNGLQMKSDADHGGVVDHVTYSKICMRRLKRPLA
FDTFYKPSNGNSYPLFKNIVLQDIHVLESPVFGAGQLLFMGILGSGNNLPMTLSMDNVVFDGFLPTLIAPPSSVVFANPQ
AVHFHFGPGPVSFAPLITPSVAYDVTVSGSPGVGNPYDCSAAFINFSSVFPDSPI

Sequences:

>Translated_535_residues
MFGLHCFAAMGGTADVFRVSQLSSSDLSEPVSVKTLWGEVQRPSLPTHVCTVLPARLTPKHGSIDALDANPKVSKPDTKR
LQDAIDDCPAGSAVKLVIDSHRKSGFLSGPLHLKSGVTLWIDEGVTLFASRNPKDYDKGNGTCGTATSTHEFSCMPLISA
INTTGSGIVGGGVIDGRGGSILTGGKYARQRTWWDLAYQNKRHALHQQVPRLIQIRGGNDFTLYRVAIENAPNFHVVADT
VSGVTAWGIRILTPSLVYTTPGYHCPPGTTPDVVTPATCFTPDTVKNTDGFDPGQSNHVLLAYSYISTGDDHVAIKARGK
MPSYALSFLHNHFGYGHGMSIGSDTESGVHDMEVSDLSIDGFDSPNSNGLQMKSDADHGGVVDHVTYSKICMRRLKRPLA
FDTFYKPSNGNSYPLFKNIVLQDIHVLESPVFGAGQLLFMGILGSGNNLPMTLSMDNVVFDGFLPTLIAPPSSVVFANPQ
AVHFHFGPGPVSFAPLITPSVAYDVTVSGSPGVGNPYDCSAAFINFSSVFPDSPI
>Mature_535_residues
MFGLHCFAAMGGTADVFRVSQLSSSDLSEPVSVKTLWGEVQRPSLPTHVCTVLPARLTPKHGSIDALDANPKVSKPDTKR
LQDAIDDCPAGSAVKLVIDSHRKSGFLSGPLHLKSGVTLWIDEGVTLFASRNPKDYDKGNGTCGTATSTHEFSCMPLISA
INTTGSGIVGGGVIDGRGGSILTGGKYARQRTWWDLAYQNKRHALHQQVPRLIQIRGGNDFTLYRVAIENAPNFHVVADT
VSGVTAWGIRILTPSLVYTTPGYHCPPGTTPDVVTPATCFTPDTVKNTDGFDPGQSNHVLLAYSYISTGDDHVAIKARGK
MPSYALSFLHNHFGYGHGMSIGSDTESGVHDMEVSDLSIDGFDSPNSNGLQMKSDADHGGVVDHVTYSKICMRRLKRPLA
FDTFYKPSNGNSYPLFKNIVLQDIHVLESPVFGAGQLLFMGILGSGNNLPMTLSMDNVVFDGFLPTLIAPPSSVVFANPQ
AVHFHFGPGPVSFAPLITPSVAYDVTVSGSPGVGNPYDCSAAFINFSSVFPDSPI

Specific function: Contributes to the wilt disease production on tomato [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 28 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 57184; Mature: 57184

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00502 POLYGALACTURONASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGLHCFAAMGGTADVFRVSQLSSSDLSEPVSVKTLWGEVQRPSLPTHVCTVLPARLTPK
CCCEEEEECCCCCHHHEEEHHCCCCCCCCCEEEEEHHCCCCCCCCCHHHHHHCCCCCCCC
HGSIDALDANPKVSKPDTKRLQDAIDDCPAGSAVKLVIDSHRKSGFLSGPLHLKSGVTLW
CCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEECCCCEEE
IDEGVTLFASRNPKDYDKGNGTCGTATSTHEFSCMPLISAINTTGSGIVGGGVIDGRGGS
EECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCEEECCCCC
ILTGGKYARQRTWWDLAYQNKRHALHQQVPRLIQIRGGNDFTLYRVAIENAPNFHVVADT
EEECCHHHHHCEEEHHHHCCHHHHHHHHCCEEEEEECCCCEEEEEEEECCCCCEEEEEEC
VSGVTAWGIRILTPSLVYTTPGYHCPPGTTPDVVTPATCFTPDTVKNTDGFDPGQSNHVL
CCCHHHCCEEEECCEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEE
LAYSYISTGDDHVAIKARGKMPSYALSFLHNHFGYGHGMSIGSDTESGVHDMEVSDLSID
EEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECEEEEC
GFDSPNSNGLQMKSDADHGGVVDHVTYSKICMRRLKRPLAFDTFYKPSNGNSYPLFKNIV
CCCCCCCCCEEECCCCCCCCEEHHHHHHHHHHHHHCCCCEEECEECCCCCCCCCHHHHHH
LQDIHVLESPVFGAGQLLFMGILGSGNNLPMTLSMDNVVFDGFLPTLIAPPSSVVFANPQ
HHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEEECCEEECCCHHHHCCCCCCEEEECCC
AVHFHFGPGPVSFAPLITPSVAYDVTVSGSPGVGNPYDCSAAFINFSSVFPDSPI
EEEEEECCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCEEEEEHHHCCCCCCC
>Mature Secondary Structure
MFGLHCFAAMGGTADVFRVSQLSSSDLSEPVSVKTLWGEVQRPSLPTHVCTVLPARLTPK
CCCEEEEECCCCCHHHEEEHHCCCCCCCCCEEEEEHHCCCCCCCCCHHHHHHCCCCCCCC
HGSIDALDANPKVSKPDTKRLQDAIDDCPAGSAVKLVIDSHRKSGFLSGPLHLKSGVTLW
CCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEECCCCEEE
IDEGVTLFASRNPKDYDKGNGTCGTATSTHEFSCMPLISAINTTGSGIVGGGVIDGRGGS
EECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCEEECCCCC
ILTGGKYARQRTWWDLAYQNKRHALHQQVPRLIQIRGGNDFTLYRVAIENAPNFHVVADT
EEECCHHHHHCEEEHHHHCCHHHHHHHHCCEEEEEECCCCEEEEEEEECCCCCEEEEEEC
VSGVTAWGIRILTPSLVYTTPGYHCPPGTTPDVVTPATCFTPDTVKNTDGFDPGQSNHVL
CCCHHHCCEEEECCEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEE
LAYSYISTGDDHVAIKARGKMPSYALSFLHNHFGYGHGMSIGSDTESGVHDMEVSDLSID
EEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECEEEEC
GFDSPNSNGLQMKSDADHGGVVDHVTYSKICMRRLKRPLAFDTFYKPSNGNSYPLFKNIV
CCCCCCCCCEEECCCCCCCCEEHHHHHHHHHHHHHCCCCEEECEECCCCCCCCCHHHHHH
LQDIHVLESPVFGAGQLLFMGILGSGNNLPMTLSMDNVVFDGFLPTLIAPPSSVVFANPQ
HHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEEECCEEECCCHHHHCCCCCCEEEECCC
AVHFHFGPGPVSFAPLITPSVAYDVTVSGSPGVGNPYDCSAAFINFSSVFPDSPI
EEEEEECCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCEEEEEHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2193922 [H]