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The map label for this gene is dpnM [H]

Identifier: 77465767

GI number: 77465767

Start: 889223

End: 890029

Strand: Reverse

Name: dpnM [H]

Synonym: RSP_3769

Alternate gene names: 77465767

Gene position: 890029-889223 (Counterclockwise)

Preceding gene: 77465768

Following gene: 77465766

Centisome position: 94.38

GC content: 66.91

Gene sequence:

>807_bases
ATGAAACAAGTACCTCCCGCCGCCCCGGTTGCCCCCTGGCTCGGCGGCAAGAAACGTCTCCACCCGCTCATCCTCGAGCG
GATCGAGGCCATCCCGCACCGCGCCTATGTCGAGCCCTTCGTCGGCATGGGCGGGATCTTCCTCCGCCGCCGCTTCCGGC
CCCGCCTCGAGGTGATGAACGACCGCAACGGCGAGATCATCAATCTCTTCCGGATCCTGCAGCGGCATTACCCCCAACTC
CTCGAGATCATGCGGTTCCAGATCTGCAGCCGGCGCGAGTTCGATCGGCTGCGGCTCACCGATCCCGCCACGCTCACCGA
CCTCGAGCGGGCGGCGCGGTTCCTCTACCTCCAGCGCCTCAGCTTCGGCGGCAAGCCGAACGGCAGCTTCGGCATATCGC
CCGGCAATGGGCCGCTCTTCTCGCTGGCGCGCCTCGAGCCGCTCCTCGACGCCGCCCGCACGCGGCTCGATGGCGTGGTC
TTCGAGTGCCTCGACTGGGCCGACCTCATCCCGCGCTACGACACAGCCGAGACGCTTTTCTATCTCGATCCGCCCTACTT
CGGCGGCGAGAACGACTATGGGAAAGGTCTCTTCGACCGGGCGCAGTTCGCGCGGATCGCCGAGATCCTCGGCAGCCTCA
AGGGCGCCTTCCTCCTGTCGATCAATGACACGCCCGAGATCCGGGCGCTCTTCGGCCGGTTCCATCTCGAGCCGGTGCGG
CTGAATTACTCGGTCTCCGCTGCAGGCAGCACCGAGGCGCAGGAGCTCCTCGTCTCGAACCGCGAGCGGATCGCGACCCT
CCTCTGA

Upstream 100 bases:

>100_bases
GGCCAATGTGCAACCAGCGACCGCGCCAGCCTGTCCGAAGCCTTTCAGACTGCCTGCCACCCCTCGCGAGGGCAGGCGCC
TTGTGAGGCAGAATCACCTT

Downstream 100 bases:

>100_bases
AAAACCCCACCAGTCCGACCACCCACGCCCCGCCCTCGCGCGGGGCTTTTGCATATGGAGAAAGACGTGACGACATCCGA
CATCCAGCGGCTGCTCGCGG

Product: adenine-specific DNA methyltransferase

Products: NA

Alternate protein names: M.DpnIIA; Adenine-specific methyltransferase DpnIIA; M.DpnII 1 [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKQVPPAAPVAPWLGGKKRLHPLILERIEAIPHRAYVEPFVGMGGIFLRRRFRPRLEVMNDRNGEIINLFRILQRHYPQL
LEIMRFQICSRREFDRLRLTDPATLTDLERAARFLYLQRLSFGGKPNGSFGISPGNGPLFSLARLEPLLDAARTRLDGVV
FECLDWADLIPRYDTAETLFYLDPPYFGGENDYGKGLFDRAQFARIAEILGSLKGAFLLSINDTPEIRALFGRFHLEPVR
LNYSVSAAGSTEAQELLVSNRERIATLL

Sequences:

>Translated_268_residues
MKQVPPAAPVAPWLGGKKRLHPLILERIEAIPHRAYVEPFVGMGGIFLRRRFRPRLEVMNDRNGEIINLFRILQRHYPQL
LEIMRFQICSRREFDRLRLTDPATLTDLERAARFLYLQRLSFGGKPNGSFGISPGNGPLFSLARLEPLLDAARTRLDGVV
FECLDWADLIPRYDTAETLFYLDPPYFGGENDYGKGLFDRAQFARIAEILGSLKGAFLLSINDTPEIRALFGRFHLEPVR
LNYSVSAAGSTEAQELLVSNRERIATLL
>Mature_268_residues
MKQVPPAAPVAPWLGGKKRLHPLILERIEAIPHRAYVEPFVGMGGIFLRRRFRPRLEVMNDRNGEIINLFRILQRHYPQL
LEIMRFQICSRREFDRLRLTDPATLTDLERAARFLYLQRLSFGGKPNGSFGISPGNGPLFSLARLEPLLDAARTRLDGVV
FECLDWADLIPRYDTAETLFYLDPPYFGGENDYGKGLFDRAQFARIAEILGSLKGAFLLSINDTPEIRALFGRFHLEPVR
LNYSVSAAGSTEAQELLVSNRERIATLL

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the DpnII endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=274, Percent_Identity=29.1970802919708, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 30514; Mature: 30514

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVPPAAPVAPWLGGKKRLHPLILERIEAIPHRAYVEPFVGMGGIFLRRRFRPRLEVMN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCHHHCCCHHHHHHHHCCHHHHHC
DRNGEIINLFRILQRHYPQLLEIMRFQICSRREFDRLRLTDPATLTDLERAARFLYLQRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHH
SFGGKPNGSFGISPGNGPLFSLARLEPLLDAARTRLDGVVFECLDWADLIPRYDTAETLF
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
YLDPPYFGGENDYGKGLFDRAQFARIAEILGSLKGAFLLSINDTPEIRALFGRFHLEPVR
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCEEE
LNYSVSAAGSTEAQELLVSNRERIATLL
EEEEEECCCCHHHHHHHHHCHHHHHHCC
>Mature Secondary Structure
MKQVPPAAPVAPWLGGKKRLHPLILERIEAIPHRAYVEPFVGMGGIFLRRRFRPRLEVMN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCHHHCCCHHHHHHHHCCHHHHHC
DRNGEIINLFRILQRHYPQLLEIMRFQICSRREFDRLRLTDPATLTDLERAARFLYLQRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHH
SFGGKPNGSFGISPGNGPLFSLARLEPLLDAARTRLDGVVFECLDWADLIPRYDTAETLF
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
YLDPPYFGGENDYGKGLFDRAQFARIAEILGSLKGAFLLSINDTPEIRALFGRFHLEPVR
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCEEE
LNYSVSAAGSTEAQELLVSNRERIATLL
EEEEEECCCCHHHHHHHHHCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2989823; 3019562; 2824782; 9862809 [H]