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The map label for this gene is phaD [H]

Identifier: 77465720

GI number: 77465720

Start: 833681

End: 835303

Strand: Reverse

Name: phaD [H]

Synonym: RSP_3713

Alternate gene names: 77465720

Gene position: 835303-833681 (Counterclockwise)

Preceding gene: 77465721

Following gene: 77465719

Centisome position: 88.58

GC content: 69.69

Gene sequence:

>1623_bases
ATGAGCGCGCACCATCTTATCGTCGCCCCGATCCTCATCCCGTTCGTGGCGGGGGCGCTGATGCTGCTCTACGACGACCG
GCAGCGGAAGGCGAAGGTCGCGATCTCGGTCGGATCGACGGTGGCGCTTCTTCTGGTGGCGGCAGAGCTTCTGGCGGGGT
CGAAGGGCAACGCGGCCTCGGGCGGCAACGACATCGGCTTCTACCTGCTCGGAGACTGGGCGGTGCCCTTCGGCATCGCG
CTGGTGATCGACCGGCTCTCGGCGCTGATGCTGCTGCTGACGGCGCTGCTGGCGATCCCGACGCTGCTCTATGCCTCGGC
CGGCTGGCACCGGCAGGGCAAGCACTTCCATTCGCTGTTCCAGTTCCTCCTGATGGGGCTGAACGGGGCCTTCCTGACCG
GCGACCTCTTCAACCTCTTCGTCTTCTTCGAGGTGCTGCTCGCGGCCTCCTACGGGCTGCTCCTGCACGGGTCGGGGCAG
CTGCGCATCCGGGCGGGGCTGCATTACATCGCGATCAACCTCGCGGCCTCGCTGCTGTTCCTGATCGGGGTCAGCCTGAT
CTACGGCGTGACCGGCACGCTCAACATGGCGCATCTGGCGCGGATCGTGCCGACGCTGGCCGAGGCCGACCTGCCGATCC
TGCATGCCGGAGCGGAGCTTCTGGGGCTTGCCTTCCTCGTGAAGGCGGGGATCTGGCCGCTCTCCTTCTGGCTGCCCACC
GCCTACATGGCGGGCGCGGCACCCGTTGCAGCCATGTTCGCGATCATGACCAAGGTGGGCGTCTATGTCATCCTGCGGGT
CTCGATGCTGCTCTTCGGGCCGGCGGCAGCCGCCTCCGCGGGCTTCGGATCGGGGGTGCTGATCGCGGGCGGCATGGCCA
CCGTCGCCTTCGGCCTGCTGGGCGTGCTGGCCTCGCAGGGGCTCGGACGGATGACGGCCAATATGGTGCTGATCTCGTCG
GGCACCGTGCTGGGCGTCGTGGGCTTCGCGCTCGCGGGCGGCGGCCCCGAGATGCTGGGCGGCGCGCTCTATTACCTCGT
CAGTTCGACCCTCGCCACCGGGGCGCTCTTCCTGCTCATCGAGCCGATGAGCCGCGAGGAGGGCGGCATCGCCGCCATGC
TGGCCCTGACCGCCGATGCCTACGGGCTCGACGAGGCCGAGGAGGACGAGCCCGAGGTGGGGCTCGCCATCCCGGGCACG
CTGACCGTGCTGGGGCTGAGCTTCGGCGCCTGTGTCCTCGCGCTGGCGGGCCTGCCGCCGCTGTCGGGCTTCATCGGCAA
GATCGCGATGCTGGCGCCCCTGCTGGCCGCGCCGGGCTTCGATCCGCTCCTCGGCTGGGCCTTCCTGTCGCTCCTGTTCC
TTGCCGGGTTCGCCACCCTGATCGGCCTCGTGCGGATCGGCATCCAGACCTTCTGGGCCACCGACGGTTCGGTGCCGAAG
GTGCTCGCGCTCGAGATCGCGCCGGTGCTGGGGCTGATCGCGCTGCTCGTGACGCTGACCGTCCGGGCCGAGGATACGCT
GCGCTATATGCAGGCCACCGCGCGGGCGCTGCACGAGCCTGCCATCTATGCCGACGGCGTCTTCGCCGCCCCGCGCGTCA
CCGAGGGGAGCGCCGCCCCATGA

Upstream 100 bases:

>100_bases
TCGTCATCAGCTTCGCCACCACCGCGCTCTTTCTCGTGGTGATGATGGCCTCGCGCGGGGTGACGGGCACCGACCATGTC
GACGGAAAGGAGCGCGAGGA

Downstream 100 bases:

>100_bases
GCCGTCTTGTGCCCTATCCGCTCCTGTCGCTGGCGCTTCTCGTGATGTGGCTTCTGCTCACGCGCTTCTCGCTGGGCCAC
CTGATCCTCGGCAGCGGCAT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSAHHLIVAPILIPFVAGALMLLYDDRQRKAKVAISVGSTVALLLVAAELLAGSKGNAASGGNDIGFYLLGDWAVPFGIA
LVIDRLSALMLLLTALLAIPTLLYASAGWHRQGKHFHSLFQFLLMGLNGAFLTGDLFNLFVFFEVLLAASYGLLLHGSGQ
LRIRAGLHYIAINLAASLLFLIGVSLIYGVTGTLNMAHLARIVPTLAEADLPILHAGAELLGLAFLVKAGIWPLSFWLPT
AYMAGAAPVAAMFAIMTKVGVYVILRVSMLLFGPAAAASAGFGSGVLIAGGMATVAFGLLGVLASQGLGRMTANMVLISS
GTVLGVVGFALAGGGPEMLGGALYYLVSSTLATGALFLLIEPMSREEGGIAAMLALTADAYGLDEAEEDEPEVGLAIPGT
LTVLGLSFGACVLALAGLPPLSGFIGKIAMLAPLLAAPGFDPLLGWAFLSLLFLAGFATLIGLVRIGIQTFWATDGSVPK
VLALEIAPVLGLIALLVTLTVRAEDTLRYMQATARALHEPAIYADGVFAAPRVTEGSAAP

Sequences:

>Translated_540_residues
MSAHHLIVAPILIPFVAGALMLLYDDRQRKAKVAISVGSTVALLLVAAELLAGSKGNAASGGNDIGFYLLGDWAVPFGIA
LVIDRLSALMLLLTALLAIPTLLYASAGWHRQGKHFHSLFQFLLMGLNGAFLTGDLFNLFVFFEVLLAASYGLLLHGSGQ
LRIRAGLHYIAINLAASLLFLIGVSLIYGVTGTLNMAHLARIVPTLAEADLPILHAGAELLGLAFLVKAGIWPLSFWLPT
AYMAGAAPVAAMFAIMTKVGVYVILRVSMLLFGPAAAASAGFGSGVLIAGGMATVAFGLLGVLASQGLGRMTANMVLISS
GTVLGVVGFALAGGGPEMLGGALYYLVSSTLATGALFLLIEPMSREEGGIAAMLALTADAYGLDEAEEDEPEVGLAIPGT
LTVLGLSFGACVLALAGLPPLSGFIGKIAMLAPLLAAPGFDPLLGWAFLSLLFLAGFATLIGLVRIGIQTFWATDGSVPK
VLALEIAPVLGLIALLVTLTVRAEDTLRYMQATARALHEPAIYADGVFAAPRVTEGSAAP
>Mature_539_residues
SAHHLIVAPILIPFVAGALMLLYDDRQRKAKVAISVGSTVALLLVAAELLAGSKGNAASGGNDIGFYLLGDWAVPFGIAL
VIDRLSALMLLLTALLAIPTLLYASAGWHRQGKHFHSLFQFLLMGLNGAFLTGDLFNLFVFFEVLLAASYGLLLHGSGQL
RIRAGLHYIAINLAASLLFLIGVSLIYGVTGTLNMAHLARIVPTLAEADLPILHAGAELLGLAFLVKAGIWPLSFWLPTA
YMAGAAPVAAMFAIMTKVGVYVILRVSMLLFGPAAAASAGFGSGVLIAGGMATVAFGLLGVLASQGLGRMTANMVLISSG
TVLGVVGFALAGGGPEMLGGALYYLVSSTLATGALFLLIEPMSREEGGIAAMLALTADAYGLDEAEEDEPEVGLAIPGTL
TVLGLSFGACVLALAGLPPLSGFIGKIAMLAPLLAAPGFDPLLGWAFLSLLFLAGFATLIGLVRIGIQTFWATDGSVPKV
LALEIAPVLGLIALLVTLTVRAEDTLRYMQATARALHEPAIYADGVFAAPRVTEGSAAP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=368, Percent_Identity=28.5326086956522, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788829, Length=448, Percent_Identity=25.6696428571429, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788827, Length=387, Percent_Identity=28.9405684754522, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 55702; Mature: 55571

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHHLIVAPILIPFVAGALMLLYDDRQRKAKVAISVGSTVALLLVAAELLAGSKGNAAS
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCC
GGNDIGFYLLGDWAVPFGIALVIDRLSALMLLLTALLAIPTLLYASAGWHRQGKHFHSLF
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
QFLLMGLNGAFLTGDLFNLFVFFEVLLAASYGLLLHGSGQLRIRAGLHYIAINLAASLLF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHHHHHHHHHHH
LIGVSLIYGVTGTLNMAHLARIVPTLAEADLPILHAGAELLGLAFLVKAGIWPLSFWLPT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCHHHHHHHH
AYMAGAAPVAAMFAIMTKVGVYVILRVSMLLFGPAAAASAGFGSGVLIAGGMATVAFGLL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEECCHHHHHHHHH
GVLASQGLGRMTANMVLISSGTVLGVVGFALAGGGPEMLGGALYYLVSSTLATGALFLLI
HHHHHCCCCHHHHCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCEEEEEE
EPMSREEGGIAAMLALTADAYGLDEAEEDEPEVGLAIPGTLTVLGLSFGACVLALAGLPP
ECCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCC
LSGFIGKIAMLAPLLAAPGFDPLLGWAFLSLLFLAGFATLIGLVRIGIQTFWATDGSVPK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
VLALEIAPVLGLIALLVTLTVRAEDTLRYMQATARALHEPAIYADGVFAAPRVTEGSAAP
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAHHLIVAPILIPFVAGALMLLYDDRQRKAKVAISVGSTVALLLVAAELLAGSKGNAAS
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCC
GGNDIGFYLLGDWAVPFGIALVIDRLSALMLLLTALLAIPTLLYASAGWHRQGKHFHSLF
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
QFLLMGLNGAFLTGDLFNLFVFFEVLLAASYGLLLHGSGQLRIRAGLHYIAINLAASLLF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHHHHHHHHHHH
LIGVSLIYGVTGTLNMAHLARIVPTLAEADLPILHAGAELLGLAFLVKAGIWPLSFWLPT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCHHHHHHHH
AYMAGAAPVAAMFAIMTKVGVYVILRVSMLLFGPAAAASAGFGSGVLIAGGMATVAFGLL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEECCHHHHHHHHH
GVLASQGLGRMTANMVLISSGTVLGVVGFALAGGGPEMLGGALYYLVSSTLATGALFLLI
HHHHHCCCCHHHHCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCEEEEEE
EPMSREEGGIAAMLALTADAYGLDEAEEDEPEVGLAIPGTLTVLGLSFGACVLALAGLPP
ECCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCC
LSGFIGKIAMLAPLLAAPGFDPLLGWAFLSLLFLAGFATLIGLVRIGIQTFWATDGSVPK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
VLALEIAPVLGLIALLVTLTVRAEDTLRYMQATARALHEPAIYADGVFAAPRVTEGSAAP
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]