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The map label for this gene is stcD2 [H]

Identifier: 77465688

GI number: 77465688

Start: 801322

End: 803388

Strand: Direct

Name: stcD2 [H]

Synonym: RSP_3681

Alternate gene names: 77465688

Gene position: 801322-803388 (Clockwise)

Preceding gene: 77465685

Following gene: 77465690

Centisome position: 84.97

GC content: 68.41

Gene sequence:

>2067_bases
ATGATGCATCCGTCCCGCCAAGGAGTCTCCCATGTCGAACGACCCCCTCCTCCAGCCCTACCGGCTCAAGCATCTCACGC
TGCGCAACCGCATCCTCATCACCAGCCACGAGCCCGCCTATCCCGAGGACGGGATGCCGAAGGACCGCTATCGCGCCTAT
CATGTCGAGCGGGCGAAGGCGGGCGTGGCCCTGACCATGACGGCGGGATCGGCCAGCGTCTCGCGCGACAGTCCGCCCGT
CTTCAACAACGTCCTGGTCTGGAAGGACGAGGTGGTCGGCTGGATGAAAAAGCTTGCCGACGAATGCCACGACCATGGCG
CGGCGGTGATATCCAGCTCACCCATCTCGGGCGGCGCACGCGGTGGGACAAGGGCGACTGGCTTCCGGTGGTGGCGCCGA
GCCACGAGCGCGAGGCGGCCCACCGCGCCTTCCCCAAGCGGATGGAGGAGTGGGACATCGCCCGCATCGTGGGCGACTTC
GCCGACGCGGCCGAGCGGATGAAGGCGGCGGGCCTCGACGGGATCGAGCTCGAAGCCTACGGCCACCTGCTCGAGCAGTT
CTGGTCGCCTCTGACCAACGATCTCGAGGCGCCGTGGGGTGGCAGCCTCGAGAACCGGTTGCGCTTCACCTTCGAGGTGC
TCGGCGCGATCCGCAGGCGCGTAGGGCCCGATTTCATCGTGGGCGTCCGCTACACGGGCGATCAGGATCTGGCGGGGGGG
ATGGGCCCGGCCGAGGGGCTCGAGGTCTCGCGCCGGCTGAAGGAGAGCGGGTTGATCGATTTCCTGAACGTCGTGAAGGG
ACATATCGACACCGACCCCGGCCTTACCGACCTGATCCCGATCCAGGGGATGCGCAGCGCGCCCCATCTCGATTTCGCGG
GCGAGATCCGCGCCGCCACCGGTTTCCCCACCTTCCATGCGGCGCGCATTCCCGACGTGGCAACCGCGCGCCACGCCATT
GCCAGCGGCAAGCTCGACATGGTGGGCATGACGCGCGCCCACCTGGCCGACCCGCATCTCGTGCGCAAGATCGTCGAAGG
CCGCGAAGACGACATCCGCCCCTGCGTCGGCGCGAACTACTGCCTCGACCGGATCTATCAGGGCGGCATGGCGCTCTGCC
TGCACAACCCCTCCAGCGGGCGCGAGATCGAGCAGCCCCACGAGATCGCCCCTGCCCCGGTAAAAAAACGTATCACCGTG
GTGGGCGCCGGCCCGGCCGGCCTCGAGGCCGCGCGCGTGGCCGGCAGCCGGGGACATGCGGTGACGGTGTTCGAAGCGGC
GTCCGAGGCCGGCGGTCAGCTGCGGCTCGCCGCGCGGCAGGAGCGACGGCGCGAGATGATGTCCATAGTGGGCTGGCGTC
TCGACCAGTGCGGCAAGCTGGGGGTGAAATTTCATTTCAACAGCTTCGCCGAGGCCGAGGCGGTGCTTGCGACAGAGCCC
GATGCGGTCATCGTGGCCACCGGGGGCCTGCCGCATACCGAGGTGCTGGAAGAGGGCAACGACCTCGTCTGCTCGGCCTG
GGACATCCTCTCGGGCGATGTGAAACCGGGTGCCAGCGCCCTCGTCTTCGACGATGCGGGCGATCATGCGGGGCTTCAGG
CGGCCGACCTCATAGCCGCGACCGGAGCCGCGGTCGAGGTCGTGACACCCGACCGCAGCTTCGCACCCGAGGTGATGGCG
ATGAACCTCGTGCCCTACATGCGCAGCCTGCAGGCGCGCGACGCAACCTTCACCGTGACATGGCGCCTCAAGTCGGTCCG
GCGCGAAGGCAACCGCCTGCGCGCGGTGCTGGGCAGCGACTACGGCCCCATGCTGCGGGACCGGCTGGTCGATCAGGTGG
TGGTCAACCACGGCACGCGGCCGCTCGACGATCTCTATTTCGAGCTGAAGCCGCTCTCGTCGAACCTGGGCGAGGTAGAT
TACGACGCCCTTGCCACGGGGGGCTCGCAGGAGCGGCTGATCAATCCCGAGGGCCGCTTCCGCCTCTACCGGATCGGAGA
CGCGGTGGCTGCGCGCAACACCCACGCCGCGATCTATGACGCGCTGCGGCTGGTGCGCGACCTCTGA

Upstream 100 bases:

>100_bases
GCTTAGGCGGGCGACAGCCGGCAGGCAAGCGAATGTTCGCCACAGATCATCCATTGGTCACTGCCGAACCTTTAATTGAC
ACAACTGAACATTTTGGCAG

Downstream 100 bases:

>100_bases
CGGCCGCAGGCAGGCCCCGAGACGGGGCCTCCGCAGGTCCCTCGGGGGCCGCGCCTCGGGTCACTGCAGGAGCCGCGATC
CGAAATCGATCAGCACGCCG

Product: putative NADH-dependent oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MMHPSRQGVSHVERPPPPALPAQASHAAQPHPHHQPRARLSRGRDAEGPLSRLSCRAGEGGRGPDHDGGIGQRLARQSAR
LQQRPGLEGRGGRLDEKACRRMPRPWRGGDIQLTHLGRRTRWDKGDWLPVVAPSHEREAAHRAFPKRMEEWDIARIVGDF
ADAAERMKAAGLDGIELEAYGHLLEQFWSPLTNDLEAPWGGSLENRLRFTFEVLGAIRRRVGPDFIVGVRYTGDQDLAGG
MGPAEGLEVSRRLKESGLIDFLNVVKGHIDTDPGLTDLIPIQGMRSAPHLDFAGEIRAATGFPTFHAARIPDVATARHAI
ASGKLDMVGMTRAHLADPHLVRKIVEGREDDIRPCVGANYCLDRIYQGGMALCLHNPSSGREIEQPHEIAPAPVKKRITV
VGAGPAGLEAARVAGSRGHAVTVFEAASEAGGQLRLAARQERRREMMSIVGWRLDQCGKLGVKFHFNSFAEAEAVLATEP
DAVIVATGGLPHTEVLEEGNDLVCSAWDILSGDVKPGASALVFDDAGDHAGLQAADLIAATGAAVEVVTPDRSFAPEVMA
MNLVPYMRSLQARDATFTVTWRLKSVRREGNRLRAVLGSDYGPMLRDRLVDQVVVNHGTRPLDDLYFELKPLSSNLGEVD
YDALATGGSQERLINPEGRFRLYRIGDAVAARNTHAAIYDALRLVRDL

Sequences:

>Translated_688_residues
MMHPSRQGVSHVERPPPPALPAQASHAAQPHPHHQPRARLSRGRDAEGPLSRLSCRAGEGGRGPDHDGGIGQRLARQSAR
LQQRPGLEGRGGRLDEKACRRMPRPWRGGDIQLTHLGRRTRWDKGDWLPVVAPSHEREAAHRAFPKRMEEWDIARIVGDF
ADAAERMKAAGLDGIELEAYGHLLEQFWSPLTNDLEAPWGGSLENRLRFTFEVLGAIRRRVGPDFIVGVRYTGDQDLAGG
MGPAEGLEVSRRLKESGLIDFLNVVKGHIDTDPGLTDLIPIQGMRSAPHLDFAGEIRAATGFPTFHAARIPDVATARHAI
ASGKLDMVGMTRAHLADPHLVRKIVEGREDDIRPCVGANYCLDRIYQGGMALCLHNPSSGREIEQPHEIAPAPVKKRITV
VGAGPAGLEAARVAGSRGHAVTVFEAASEAGGQLRLAARQERRREMMSIVGWRLDQCGKLGVKFHFNSFAEAEAVLATEP
DAVIVATGGLPHTEVLEEGNDLVCSAWDILSGDVKPGASALVFDDAGDHAGLQAADLIAATGAAVEVVTPDRSFAPEVMA
MNLVPYMRSLQARDATFTVTWRLKSVRREGNRLRAVLGSDYGPMLRDRLVDQVVVNHGTRPLDDLYFELKPLSSNLGEVD
YDALATGGSQERLINPEGRFRLYRIGDAVAARNTHAAIYDALRLVRDL
>Mature_688_residues
MMHPSRQGVSHVERPPPPALPAQASHAAQPHPHHQPRARLSRGRDAEGPLSRLSCRAGEGGRGPDHDGGIGQRLARQSAR
LQQRPGLEGRGGRLDEKACRRMPRPWRGGDIQLTHLGRRTRWDKGDWLPVVAPSHEREAAHRAFPKRMEEWDIARIVGDF
ADAAERMKAAGLDGIELEAYGHLLEQFWSPLTNDLEAPWGGSLENRLRFTFEVLGAIRRRVGPDFIVGVRYTGDQDLAGG
MGPAEGLEVSRRLKESGLIDFLNVVKGHIDTDPGLTDLIPIQGMRSAPHLDFAGEIRAATGFPTFHAARIPDVATARHAI
ASGKLDMVGMTRAHLADPHLVRKIVEGREDDIRPCVGANYCLDRIYQGGMALCLHNPSSGREIEQPHEIAPAPVKKRITV
VGAGPAGLEAARVAGSRGHAVTVFEAASEAGGQLRLAARQERRREMMSIVGWRLDQCGKLGVKFHFNSFAEAEAVLATEP
DAVIVATGGLPHTEVLEEGNDLVCSAWDILSGDVKPGASALVFDDAGDHAGLQAADLIAATGAAVEVVTPDRSFAPEVMA
MNLVPYMRSLQARDATFTVTWRLKSVRREGNRLRAVLGSDYGPMLRDRLVDQVVVNHGTRPLDDLYFELKPLSSNLGEVD
YDALATGGSQERLINPEGRFRLYRIGDAVAARNTHAAIYDALRLVRDL

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=386, Percent_Identity=33.419689119171, Blast_Score=187, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17559804, Length=256, Percent_Identity=28.90625, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=255, Percent_Identity=28.6274509803922, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI72001454, Length=254, Percent_Identity=27.9527559055118, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17559802, Length=254, Percent_Identity=29.5275590551181, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17565138, Length=241, Percent_Identity=28.2157676348548, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=209, Percent_Identity=31.5789473684211, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321973, Length=207, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 74968; Mature: 74968

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHPSRQGVSHVERPPPPALPAQASHAAQPHPHHQPRARLSRGRDAEGPLSRLSCRAGEG
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHCCCCCC
GRGPDHDGGIGQRLARQSARLQQRPGLEGRGGRLDEKACRRMPRPWRGGDIQLTHLGRRT
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCC
RWDKGDWLPVVAPSHEREAAHRAFPKRMEEWDIARIVGDFADAAERMKAAGLDGIELEAY
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
GHLLEQFWSPLTNDLEAPWGGSLENRLRFTFEVLGAIRRRVGPDFIVGVRYTGDQDLAGG
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
MGPAEGLEVSRRLKESGLIDFLNVVKGHIDTDPGLTDLIPIQGMRSAPHLDFAGEIRAAT
CCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECC
GFPTFHAARIPDVATARHAIASGKLDMVGMTRAHLADPHLVRKIVEGREDDIRPCVGANY
CCCCCHHCCCCCHHHHHHHHHCCCEEEEECHHHHCCCHHHHHHHHCCCCCCCCCCCCHHH
CLDRIYQGGMALCLHNPSSGREIEQPHEIAPAPVKKRITVVGAGPAGLEAARVAGSRGHA
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCCCE
VTVFEAASEAGGQLRLAARQERRREMMSIVGWRLDQCGKLGVKFHFNSFAEAEAVLATEP
EEEEECCHHCCCEEEEHHHHHHHHHHHHHHHHCHHCCCCCCEEEECCCCCCCCEEEECCC
DAVIVATGGLPHTEVLEEGNDLVCSAWDILSGDVKPGASALVFDDAGDHAGLQAADLIAA
CEEEEEECCCCHHHHHHCCCCEEEEEHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHH
TGAAVEVVTPDRSFAPEVMAMNLVPYMRSLQARDATFTVTWRLKSVRREGNRLRAVLGSD
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHCCCCEEHHHCCC
YGPMLRDRLVDQVVVNHGTRPLDDLYFELKPLSSNLGEVDYDALATGGSQERLINPEGRF
CCHHHHHHHHHHHHHHCCCCCHHHHHEEEECCCCCCCCCCHHHHHCCCCCCCEECCCCCE
RLYRIGDAVAARNTHAAIYDALRLVRDL
EEEEECCHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMHPSRQGVSHVERPPPPALPAQASHAAQPHPHHQPRARLSRGRDAEGPLSRLSCRAGEG
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHCCCCCC
GRGPDHDGGIGQRLARQSARLQQRPGLEGRGGRLDEKACRRMPRPWRGGDIQLTHLGRRT
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCC
RWDKGDWLPVVAPSHEREAAHRAFPKRMEEWDIARIVGDFADAAERMKAAGLDGIELEAY
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
GHLLEQFWSPLTNDLEAPWGGSLENRLRFTFEVLGAIRRRVGPDFIVGVRYTGDQDLAGG
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
MGPAEGLEVSRRLKESGLIDFLNVVKGHIDTDPGLTDLIPIQGMRSAPHLDFAGEIRAAT
CCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECC
GFPTFHAARIPDVATARHAIASGKLDMVGMTRAHLADPHLVRKIVEGREDDIRPCVGANY
CCCCCHHCCCCCHHHHHHHHHCCCEEEEECHHHHCCCHHHHHHHHCCCCCCCCCCCCHHH
CLDRIYQGGMALCLHNPSSGREIEQPHEIAPAPVKKRITVVGAGPAGLEAARVAGSRGHA
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCCCE
VTVFEAASEAGGQLRLAARQERRREMMSIVGWRLDQCGKLGVKFHFNSFAEAEAVLATEP
EEEEECCHHCCCEEEEHHHHHHHHHHHHHHHHCHHCCCCCCEEEECCCCCCCCEEEECCC
DAVIVATGGLPHTEVLEEGNDLVCSAWDILSGDVKPGASALVFDDAGDHAGLQAADLIAA
CEEEEEECCCCHHHHHHCCCCEEEEEHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHH
TGAAVEVVTPDRSFAPEVMAMNLVPYMRSLQARDATFTVTWRLKSVRREGNRLRAVLGSD
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHCCCCEEHHHCCC
YGPMLRDRLVDQVVVNHGTRPLDDLYFELKPLSSNLGEVDYDALATGGSQERLINPEGRF
CCHHHHHHHHHHHHHHCCCCCHHHHHEEEECCCCCCCCCCHHHHHCCCCCCCEECCCCCE
RLYRIGDAVAARNTHAAIYDALRLVRDL
EEEEECCHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]