Click here to switch to the map view.

The map label for this gene is cspA [C]

Identifier: 77465631

GI number: 77465631

Start: 733277

End: 733510

Strand: Direct

Name: cspA [C]

Synonym: RSP_3621

Alternate gene names: 77465631

Gene position: 733277-733510 (Clockwise)

Preceding gene: 77465629

Following gene: 77465632

Centisome position: 77.76

GC content: 61.97

Gene sequence:

>234_bases
GTGGCGAACAAGACAGGAGAGATCACGATGGCCAATGGCACCGTGAAATGGTTCAACGCAACTAAAGGGTTCGGCTTCAT
CGCGCCGACGGGCGGCTCGAAGGACGTTTTCGTCCATGTGACCGCGCTTGAGCGCGCTGGCATTCGGCAACTCGACGATG
GCCAGGCGGTGAGCTTCGACCTCGAGCGCGATCGCAACGGCCGCGAGTCGGCGACGAACCTCGTTCTCGCCTGA

Upstream 100 bases:

>100_bases
GTCATAAGGGCCGGGCTAAGCATCCTCTTTCGAACTTCTGTTTCCTTGCGGCTTCAGTTGCGACTCTGACGAAACTGAGC
CAGGCCACCGCATCACGCGG

Downstream 100 bases:

>100_bases
GCGACGACTGCTGAGACCGGAAAGCGGGGCGGCTTGAAGCTGCCCGCTTTCATTCGAACCGCAGCGGCCTCTCAGGCTGC
GCGGTTCTCTTTATCCGAAA

Product: cold-shock DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 77; Mature: 76

Protein sequence:

>77_residues
MANKTGEITMANGTVKWFNATKGFGFIAPTGGSKDVFVHVTALERAGIRQLDDGQAVSFDLERDRNGRESATNLVLA

Sequences:

>Translated_77_residues
MANKTGEITMANGTVKWFNATKGFGFIAPTGGSKDVFVHVTALERAGIRQLDDGQAVSFDLERDRNGRESATNLVLA
>Mature_76_residues
ANKTGEITMANGTVKWFNATKGFGFIAPTGGSKDVFVHVTALERAGIRQLDDGQAVSFDLERDRNGRESATNLVLA

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=55, Percent_Identity=60, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=51.5625, Blast_Score=70, Evalue=2e-14,
Organism=Escherichia coli, GI1787839, Length=63, Percent_Identity=50.7936507936508, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1787107, Length=63, Percent_Identity=46.031746031746, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=48.4375, Blast_Score=69, Evalue=9e-14,
Organism=Escherichia coli, GI1788126, Length=55, Percent_Identity=52.7272727272727, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787834, Length=55, Percent_Identity=50.9090909090909, Blast_Score=62, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 8271; Mature: 8140

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKTGEITMANGTVKWFNATKGFGFIAPTGGSKDVFVHVTALERAGIRQLDDGQAVSFD
CCCCCCCEEECCCEEEEEECCCCCEEEEECCCCCEEEEEEEECCCCCCEECCCCCEEEEE
LERDRNGRESATNLVLA
ECCCCCCCCCCCEEEEC
>Mature Secondary Structure 
ANKTGEITMANGTVKWFNATKGFGFIAPTGGSKDVFVHVTALERAGIRQLDDGQAVSFD
CCCCCCEEECCCEEEEEECCCCCEEEEECCCCCEEEEEEEECCCCCCEECCCCCEEEEE
LERDRNGRESATNLVLA
ECCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]