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The map label for this gene is rpoN [H]

Identifier: 77465608

GI number: 77465608

Start: 701300

End: 702598

Strand: Direct

Name: rpoN [H]

Synonym: RSP_3606

Alternate gene names: 77465608

Gene position: 701300-702598 (Clockwise)

Preceding gene: 77465607

Following gene: 77465609

Centisome position: 74.37

GC content: 73.29

Gene sequence:

>1299_bases
ATGAAGTCCCGCCAGCGCATCAGTATCGCCCAGACCCAGAGGCTGCAGCTCAATCTCGGCCTCACCGCCTCGATCCGCGT
CCTGAATTCCGATGCCGAGGGCCTCACGCGCTACCTGCAGGAGCAGGCGGCGGAGAACCCCCATATCCAGCTCGAACCGG
CAACCTCGACCGACTGGCTGCCGCGCTGGACGAGCGTTCTGTCGCGCCTCGCGCAGGGCGAGGGGTCGGCGGGCGGAGAG
ACGGTGGCGGCGGCGGGGCCGAGCCTCATGGCGCATGTGATGGCGCGCATCGACACGCTTTATCCGCGCGGGCCCGAGCG
GCGGATCGCCATCCTTCTGGCCGAAGCGCTGGAGCCCACCGGCTGGCTCGGGACGGGACCGGACGAGATCGCCCGGCAGG
CCCGCGTCCCCTCCGCCGAGGTCGAGGCCGTGCTGGCCGGGCTGCAGAAGATCGAGCCCGCCGGCCTCTTCGCCCGAACC
CTCGCCGAGTGCCTGCGGCTTCAGGCCATCGAGGCCGAGCGGCTCGATTCCACCCTGAGCTGCCTTCTCGACCATCTCGA
CCTGGTGGCAGAGGGGGCCCTCGGGCGGCTCGCGCGGCTCTGCAACACGGACGAGGCCGGGGTGACCGCGCGCCTGCGGC
TCCTGCGGACCTTCGACCCGAAGCCCGGCGCGCAGTTCGATCCGGGCGCGGCGCCGGTGCGCGAGCCCGACCTGATCGCG
ACGAAGGGCGAGGCCGGGTGGGAGGTGTCGCTGAACCGCTCGGCCATGCCCACGGTGCAGATCCGCAAGCCGGACAAGCG
CCCGACGACGCCGGCCGCCCGCGCGGCCTGGACCCAGGCGCAGGCGGTGGGCCGGATGATCGAGAACCGCAATGCCACGC
TGCTGAGGGTCGCGCGCGAGATCCTCGCCCGGCAGGAGGCGGCGCTCGACGAAGGTCCCTCGGCGCTCGTGGCCCTGACC
ATGACCGAGGTGGCCGAGGCGCTCGGCATCCACGAGAGCACGGTGAGCCGCGTGGTCGCGGGCACCTGCGTGGACACGCC
GCGCGGCACCTGGTGGCTGCGGCGCATGTTCAGCGGCCGCCTTGCCGAGGGCGGTCCCTCGGCCGCGGCCATCCGCGCCG
CCATCGCCCGCCTCGTCGCGCAGGAAGATCCGGCCGCGCCTTTGTCCGACGGCGCTCTGGTCGAGGCGCTGGCGGCCGAG
GACATGCAGCTGGCGCGCCGCACCGTCGCCAAATATCGCGAGATGCTGAACATCCCCCCCGGACACCGCCGCCGCCGCAG
GCCCTCGCGCTCGGCCTGA

Upstream 100 bases:

>100_bases
GTTCCGGGACTGATCCCGCCGGGCGGCGTGGCGCATCCAAGCGCCGGGGCTCCCGCCCCGGTTGCGCCATGGGCGCGCGG
GCACTAGGCTCGACGGAACC

Downstream 100 bases:

>100_bases
TCCGCCAGCACGACGCCGGCGGCCTGTCCCGCGTCGCATGGGGCCTTTCGGACCGCGCGCGATCGGCTAGAACCGGGACA
GTTTGTCGCGGAGACTGCCA

Product: Sigma54-2 (RNA polymerase sigma-54 factor)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKSRQRISIAQTQRLQLNLGLTASIRVLNSDAEGLTRYLQEQAAENPHIQLEPATSTDWLPRWTSVLSRLAQGEGSAGGE
TVAAAGPSLMAHVMARIDTLYPRGPERRIAILLAEALEPTGWLGTGPDEIARQARVPSAEVEAVLAGLQKIEPAGLFART
LAECLRLQAIEAERLDSTLSCLLDHLDLVAEGALGRLARLCNTDEAGVTARLRLLRTFDPKPGAQFDPGAAPVREPDLIA
TKGEAGWEVSLNRSAMPTVQIRKPDKRPTTPAARAAWTQAQAVGRMIENRNATLLRVAREILARQEAALDEGPSALVALT
MTEVAEALGIHESTVSRVVAGTCVDTPRGTWWLRRMFSGRLAEGGPSAAAIRAAIARLVAQEDPAAPLSDGALVEALAAE
DMQLARRTVAKYREMLNIPPGHRRRRRPSRSA

Sequences:

>Translated_432_residues
MKSRQRISIAQTQRLQLNLGLTASIRVLNSDAEGLTRYLQEQAAENPHIQLEPATSTDWLPRWTSVLSRLAQGEGSAGGE
TVAAAGPSLMAHVMARIDTLYPRGPERRIAILLAEALEPTGWLGTGPDEIARQARVPSAEVEAVLAGLQKIEPAGLFART
LAECLRLQAIEAERLDSTLSCLLDHLDLVAEGALGRLARLCNTDEAGVTARLRLLRTFDPKPGAQFDPGAAPVREPDLIA
TKGEAGWEVSLNRSAMPTVQIRKPDKRPTTPAARAAWTQAQAVGRMIENRNATLLRVAREILARQEAALDEGPSALVALT
MTEVAEALGIHESTVSRVVAGTCVDTPRGTWWLRRMFSGRLAEGGPSAAAIRAAIARLVAQEDPAAPLSDGALVEALAAE
DMQLARRTVAKYREMLNIPPGHRRRRRPSRSA
>Mature_432_residues
MKSRQRISIAQTQRLQLNLGLTASIRVLNSDAEGLTRYLQEQAAENPHIQLEPATSTDWLPRWTSVLSRLAQGEGSAGGE
TVAAAGPSLMAHVMARIDTLYPRGPERRIAILLAEALEPTGWLGTGPDEIARQARVPSAEVEAVLAGLQKIEPAGLFART
LAECLRLQAIEAERLDSTLSCLLDHLDLVAEGALGRLARLCNTDEAGVTARLRLLRTFDPKPGAQFDPGAAPVREPDLIA
TKGEAGWEVSLNRSAMPTVQIRKPDKRPTTPAARAAWTQAQAVGRMIENRNATLLRVAREILARQEAALDEGPSALVALT
MTEVAEALGIHESTVSRVVAGTCVDTPRGTWWLRRMFSGRLAEGGPSAAAIRAAIARLVAQEDPAAPLSDGALVEALAAE
DMQLARRTVAKYREMLNIPPGHRRRRRPSRSA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=366, Percent_Identity=32.2404371584699, Blast_Score=195, Evalue=4e-51,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 46611; Mature: 46611

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRQRISIAQTQRLQLNLGLTASIRVLNSDAEGLTRYLQEQAAENPHIQLEPATSTDWL
CCCCHHEHHHHHHEEEEEECCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCC
PRWTSVLSRLAQGEGSAGGETVAAAGPSLMAHVMARIDTLYPRGPERRIAILLAEALEPT
HHHHHHHHHHHCCCCCCCCCEEHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
GWLGTGPDEIARQARVPSAEVEAVLAGLQKIEPAGLFARTLAECLRLQAIEAERLDSTLS
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLLDHLDLVAEGALGRLARLCNTDEAGVTARLRLLRTFDPKPGAQFDPGAAPVREPDLIA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
TKGEAGWEVSLNRSAMPTVQIRKPDKRPTTPAARAAWTQAQAVGRMIENRNATLLRVARE
ECCCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ILARQEAALDEGPSALVALTMTEVAEALGIHESTVSRVVAGTCVDTPRGTWWLRRMFSGR
HHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
LAEGGPSAAAIRAAIARLVAQEDPAAPLSDGALVEALAAEDMQLARRTVAKYREMLNIPP
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GHRRRRRPSRSA
CHHHHCCCCCCC
>Mature Secondary Structure
MKSRQRISIAQTQRLQLNLGLTASIRVLNSDAEGLTRYLQEQAAENPHIQLEPATSTDWL
CCCCHHEHHHHHHEEEEEECCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCC
PRWTSVLSRLAQGEGSAGGETVAAAGPSLMAHVMARIDTLYPRGPERRIAILLAEALEPT
HHHHHHHHHHHCCCCCCCCCEEHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
GWLGTGPDEIARQARVPSAEVEAVLAGLQKIEPAGLFARTLAECLRLQAIEAERLDSTLS
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLLDHLDLVAEGALGRLARLCNTDEAGVTARLRLLRTFDPKPGAQFDPGAAPVREPDLIA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
TKGEAGWEVSLNRSAMPTVQIRKPDKRPTTPAARAAWTQAQAVGRMIENRNATLLRVARE
ECCCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ILARQEAALDEGPSALVALTMTEVAEALGIHESTVSRVVAGTCVDTPRGTWWLRRMFSGR
HHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
LAEGGPSAAAIRAAIARLVAQEDPAAPLSDGALVEALAAEDMQLARRTVAKYREMLNIPP
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GHRRRRRPSRSA
CHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317839 [H]