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The map label for this gene is bioA [C]

Identifier: 77465533

GI number: 77465533

Start: 615513

End: 616916

Strand: Reverse

Name: bioA [C]

Synonym: RSP_3534

Alternate gene names: 77465533

Gene position: 616916-615513 (Counterclockwise)

Preceding gene: 77465537

Following gene: 77465532

Centisome position: 65.42

GC content: 67.24

Gene sequence:

>1404_bases
ATGACGCGGAATGACGCGACGAATGCTGCCGGAGCGGTGGGCGCGGCGATGCGGGATCACATCCTCTTGCCTGCACAGGA
AATGGCGAAGCTCGGCAAGTCCGCGCAGCCGGTGCTGACTCATGCCGAGGGCATCTATGTCCATACCGAGGACGGCCGCC
GCCTGATCGACGGGCCGGCGGGCATGTGGTGCGCGCAGGTGGGCTACGGCCGCCGCGAGATCGTCGATGCCATGGCGCAT
CAGGCGATGGTGCTGCCCTATGCCTCGCCCTGGTATATGGCCACGAGCCCCGCGGCGCGGCTGGCGGAGAAGATCGCCAC
GCTGACGCCGGGCGATCTCAACCGGATCTTTTTCACCACGGGCGGGTCGACCGCGGTGGACAGCGCGCTGCGCTTCTCGG
AATTCTACAACAACGTGCTGGGCCGGCCGCAGAAGAAGCGCATCATCGTGCGCTACGACGGCTATCACGGCTCGACGGCG
CTCACCGCCGCCTGCACCGGCCGCACCGGCAACTGGCCGAACTTCGACATCGCGCAGGACCGGATCTCGTTCCTCTCGAG
CCCCAATCCGCGCCACGCCGGCAACCGCAGCCAGGAGGCGTTCCTCGACGATCTGGTGCAGGAATTCGAGGACCGGATCG
AGAGCCTCGGCCCCGACACGATCGCGGCCTTCCTGGCCGAGCCGATCCTCGCCTCGGGCGGCGTCATTATTCCGCCCGCA
GGCTATCATGCGCGCTTCAAGGCGATCTGCGAGAAGCACGACATCCTCTATATCTCGGACGAGGTGGTGACGGGCTTCGG
CCGTTGCGGCGAGTGGTTCGCCTCGGAGAAGGTGTTCGGGGTGGTGCCGGACATCATCACCTTCGCCAAGGGCGTGACCT
CGGGCTATGTGCCGCTCGGCGGCCTTGCGATCTCCGAGGCGGTGCTGGCGCGGATCTCGGGCGAGAATGCCAAGGGAAGC
TGGTTCACCAACGGCTATACCTACAGCAATCAGCCGGTGGCCTGCGCCGCGGCGCTTGCCAACATCGAGCTGATGGAGCG
CGAGGGCATCGTCGATCAGGCGCGCGAGATGGCGGACTATTTCGCCGCGGCGCTGGCTTCGCTGCGCGATCTGCCGGGCG
TGGCGGAAACCCGGTCGGTGGGCCTCGTGGGTTGCGTGCAATGCCTGCTCGACCCGACCCGGGCGGACGGCACGGCCGAG
GACAAGGCCTTCACCCTGAAGATCGACGAGCGCTGCTTCGAGCTCGGGCTGATCGTGCGCCCGCTGGGCGATCTCTGCGT
GATCTCGCCGCCGCTCATCATCTCGCGCGCGCAGATCGACGAGATGGTCGCGATCATGCGGCAGGCCATCACCGAAGTGA
GCGCCGCCCACGGTCTGACCGCGAAAGAACCGGCCGCCGTCTGA

Upstream 100 bases:

>100_bases
TGTTCCGCCGGAGTGCCCACTACTGGCGGCAGAGGCGGTGCCCGGTTGCGGGGGCTTGCCCGGGAATGAACCGAAATGCG
GACGAGAGGGGAGGTCGGCG

Downstream 100 bases:

>100_bases
GGCGGCCGCGACGAAACTCCCTCCAACAGGATCCCAGATGCTGCAAGAGATCGACTGGCACGCCCGCGCCAAAGCCGTGG
CCCTTCCGAACCGTCCCTTC

Product: hypothetical protein

Products: S-Adenosyl-4-methylthio-2-oxobutanoate; 7,8-Diaminononanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTRNDATNAAGAVGAAMRDHILLPAQEMAKLGKSAQPVLTHAEGIYVHTEDGRRLIDGPAGMWCAQVGYGRREIVDAMAH
QAMVLPYASPWYMATSPAARLAEKIATLTPGDLNRIFFTTGGSTAVDSALRFSEFYNNVLGRPQKKRIIVRYDGYHGSTA
LTAACTGRTGNWPNFDIAQDRISFLSSPNPRHAGNRSQEAFLDDLVQEFEDRIESLGPDTIAAFLAEPILASGGVIIPPA
GYHARFKAICEKHDILYISDEVVTGFGRCGEWFASEKVFGVVPDIITFAKGVTSGYVPLGGLAISEAVLARISGENAKGS
WFTNGYTYSNQPVACAAALANIELMEREGIVDQAREMADYFAAALASLRDLPGVAETRSVGLVGCVQCLLDPTRADGTAE
DKAFTLKIDERCFELGLIVRPLGDLCVISPPLIISRAQIDEMVAIMRQAITEVSAAHGLTAKEPAAV

Sequences:

>Translated_467_residues
MTRNDATNAAGAVGAAMRDHILLPAQEMAKLGKSAQPVLTHAEGIYVHTEDGRRLIDGPAGMWCAQVGYGRREIVDAMAH
QAMVLPYASPWYMATSPAARLAEKIATLTPGDLNRIFFTTGGSTAVDSALRFSEFYNNVLGRPQKKRIIVRYDGYHGSTA
LTAACTGRTGNWPNFDIAQDRISFLSSPNPRHAGNRSQEAFLDDLVQEFEDRIESLGPDTIAAFLAEPILASGGVIIPPA
GYHARFKAICEKHDILYISDEVVTGFGRCGEWFASEKVFGVVPDIITFAKGVTSGYVPLGGLAISEAVLARISGENAKGS
WFTNGYTYSNQPVACAAALANIELMEREGIVDQAREMADYFAAALASLRDLPGVAETRSVGLVGCVQCLLDPTRADGTAE
DKAFTLKIDERCFELGLIVRPLGDLCVISPPLIISRAQIDEMVAIMRQAITEVSAAHGLTAKEPAAV
>Mature_466_residues
TRNDATNAAGAVGAAMRDHILLPAQEMAKLGKSAQPVLTHAEGIYVHTEDGRRLIDGPAGMWCAQVGYGRREIVDAMAHQ
AMVLPYASPWYMATSPAARLAEKIATLTPGDLNRIFFTTGGSTAVDSALRFSEFYNNVLGRPQKKRIIVRYDGYHGSTAL
TAACTGRTGNWPNFDIAQDRISFLSSPNPRHAGNRSQEAFLDDLVQEFEDRIESLGPDTIAAFLAEPILASGGVIIPPAG
YHARFKAICEKHDILYISDEVVTGFGRCGEWFASEKVFGVVPDIITFAKGVTSGYVPLGGLAISEAVLARISGENAKGSW
FTNGYTYSNQPVACAAALANIELMEREGIVDQAREMADYFAAALASLRDLPGVAETRSVGLVGCVQCLLDPTRADGTAED
KAFTLKIDERCFELGLIVRPLGDLCVISPPLIISRAQIDEMVAIMRQAITEVSAAHGLTAKEPAAV

Specific function: Biotin biosynthesis. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=442, Percent_Identity=25.7918552036199, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI24119277, Length=423, Percent_Identity=26.0047281323877, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI37574042, Length=429, Percent_Identity=25.4079254079254, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI226442705, Length=429, Percent_Identity=25.1748251748252, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI226442709, Length=366, Percent_Identity=26.2295081967213, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI4557809, Length=424, Percent_Identity=23.8207547169811, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI284507298, Length=235, Percent_Identity=28.5106382978723, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1786991, Length=437, Percent_Identity=29.0617848970252, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1789016, Length=419, Percent_Identity=27.9236276849642, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI145693181, Length=406, Percent_Identity=28.0788177339901, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787560, Length=418, Percent_Identity=28.2296650717703, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1788044, Length=402, Percent_Identity=26.6169154228856, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789759, Length=449, Percent_Identity=24.9443207126949, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32564660, Length=448, Percent_Identity=28.3482142857143, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71992977, Length=432, Percent_Identity=24.3055555555556, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI25144274, Length=250, Percent_Identity=28.4, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=247, Percent_Identity=27.5303643724696, Blast_Score=109, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324386, Length=438, Percent_Identity=27.1689497716895, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6323470, Length=425, Percent_Identity=26.1176470588235, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324432, Length=414, Percent_Identity=25.3623188405797, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28574759, Length=450, Percent_Identity=26.6666666666667, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI161085790, Length=448, Percent_Identity=25.4464285714286, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21357415, Length=417, Percent_Identity=25.8992805755396, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21356575, Length=428, Percent_Identity=25.2336448598131, Blast_Score=115, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62 [C]

Molecular weight: Translated: 50156; Mature: 50024

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRNDATNAAGAVGAAMRDHILLPAQEMAKLGKSAQPVLTHAEGIYVHTEDGRRLIDGPA
CCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEECCCEEEEECCCCCEEECCCC
GMWCAQVGYGRREIVDAMAHQAMVLPYASPWYMATSPAARLAEKIATLTPGDLNRIFFTT
CHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCEECCHHHHHHHHHHHCCCCCCCEEEEEC
GGSTAVDSALRFSEFYNNVLGRPQKKRIIVRYDGYHGSTALTAACTGRTGNWPNFDIAQD
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHH
RISFLSSPNPRHAGNRSQEAFLDDLVQEFEDRIESLGPDTIAAFLAEPILASGGVIIPPA
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCC
GYHARFKAICEKHDILYISDEVVTGFGRCGEWFASEKVFGVVPDIITFAKGVTSGYVPLG
CCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
GLAISEAVLARISGENAKGSWFTNGYTYSNQPVACAAALANIELMEREGIVDQAREMADY
HHHHHHHHHHHHCCCCCCCCEEECCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
FAAALASLRDLPGVAETRSVGLVGCVQCLLDPTRADGTAEDKAFTLKIDERCFELGLIVR
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHH
PLGDLCVISPPLIISRAQIDEMVAIMRQAITEVSAAHGLTAKEPAAV
CCCCCEEECCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TRNDATNAAGAVGAAMRDHILLPAQEMAKLGKSAQPVLTHAEGIYVHTEDGRRLIDGPA
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEECCCEEEEECCCCCEEECCCC
GMWCAQVGYGRREIVDAMAHQAMVLPYASPWYMATSPAARLAEKIATLTPGDLNRIFFTT
CHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCEECCHHHHHHHHHHHCCCCCCCEEEEEC
GGSTAVDSALRFSEFYNNVLGRPQKKRIIVRYDGYHGSTALTAACTGRTGNWPNFDIAQD
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHH
RISFLSSPNPRHAGNRSQEAFLDDLVQEFEDRIESLGPDTIAAFLAEPILASGGVIIPPA
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCC
GYHARFKAICEKHDILYISDEVVTGFGRCGEWFASEKVFGVVPDIITFAKGVTSGYVPLG
CCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
GLAISEAVLARISGENAKGSWFTNGYTYSNQPVACAAALANIELMEREGIVDQAREMADY
HHHHHHHHHHHHCCCCCCCCEEECCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
FAAALASLRDLPGVAETRSVGLVGCVQCLLDPTRADGTAEDKAFTLKIDERCFELGLIVR
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHH
PLGDLCVISPPLIISRAQIDEMVAIMRQAITEVSAAHGLTAKEPAAV
CCCCCEEECCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Amino-7-oxononanoate; S-Adenosyl-L-methionine [C]

Specific reaction: 8-Amino-7-oxononanoate + S-Adenosyl-L-methionine <==> S-Adenosyl-4-methylthio-2-oxobutanoate + 7,8-Diaminononanoate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]