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The map label for this gene is hisJ [H]

Identifier: 77465459

GI number: 77465459

Start: 527231

End: 528094

Strand: Direct

Name: hisJ [H]

Synonym: RSP_3457

Alternate gene names: 77465459

Gene position: 527231-528094 (Clockwise)

Preceding gene: 77465458

Following gene: 77465460

Centisome position: 55.91

GC content: 67.71

Gene sequence:

>864_bases
ATGACCAACCCCCGCTTCGGGGGGCTTGTGGCGCTTGCCTTCATGGCCGCGACCACGCTGCCCGCCCAGTCCCAGGTGGC
CGAGATCCGCGACAGCTATCGCGTGGCGCTCGACGGCACCTTCGCTCCCCATGCCATGCCGAAACTCTCGGGCGGTGTCG
AAGGGTTCAACGTCGATCTGGCCCAGCTCATCGGCGAGCGTCTGGGGTCCGAGATGACGGTCGATGCGGCGCAATTCTCC
GGCCTCATCCCGGCGCTGCAGGCGGGCACCTACGACTTTCTCGTGGCCCCGGTCACGGTGAATCAGGAGCGGTCCGAGAA
CCTGCTCTTCACCGAAGGCTTCATGGACACGAACTTCGCCTTCGTCGTGCCGGCGGGCTCGGAGAAGCGGGACGGGATCG
AGGCCTTCAAGGGGATGACGATCTCGGTCAACCGGGGTTCGGCCTACCACACCTATCTCGACGACCATGCCGCCGAATAC
GGCTGGACCGTCGTGCCCTTCGGCACCAACACCGATGCGGTCGAGGCGGTGGCCACCGGACGCGCGGACGCGAACCTCTC
CGGCGCGACCGTGGCGGCCTGGGCCGCGCTCCAGAACCCGGCGGTCGATCTGTCCTACGAGATCCAGACCGGGCTCGTCT
GGGGGCTGGCCTTCCGGCTGGACGATGCGGCGACGCGCAACCGCGTCGATCTCGTGCTCGAATGCCTGAAGACCGACGGC
TCGCTCGCGGCCCTTTCCGAGAAATGGTTCGGCGTGACGCCCGCGGCCGGCAGTACCATCGTGACGCCGACGCCGGGCTT
CGGCGTGCCGGGCTTCCCGGGCTATCAGGAAGATGCCCACCAACCCTCCTGCGACTTCTCCTGA

Upstream 100 bases:

>100_bases
AAGAGCCCTCATTTATATTGATGCGCCGGGGGTGCCTGTGGTCAGGATGATCTGCGGCGACAGAACCGACGAACATTCCG
CCCCATCAACAGGAGATCCC

Downstream 100 bases:

>100_bases
TCGCTGCCTGCCCGCCGCCCCGTCCCCGGCGGACGGGGCGGCGCCCCACCCCATCCCATCGGAGCCCGCATGTCCCGACC
CATGCTCGAAATCGTCGCGC

Product: ABC polar amino acid transporter, periplasmic binding protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTNPRFGGLVALAFMAATTLPAQSQVAEIRDSYRVALDGTFAPHAMPKLSGGVEGFNVDLAQLIGERLGSEMTVDAAQFS
GLIPALQAGTYDFLVAPVTVNQERSENLLFTEGFMDTNFAFVVPAGSEKRDGIEAFKGMTISVNRGSAYHTYLDDHAAEY
GWTVVPFGTNTDAVEAVATGRADANLSGATVAAWAALQNPAVDLSYEIQTGLVWGLAFRLDDAATRNRVDLVLECLKTDG
SLAALSEKWFGVTPAAGSTIVTPTPGFGVPGFPGYQEDAHQPSCDFS

Sequences:

>Translated_287_residues
MTNPRFGGLVALAFMAATTLPAQSQVAEIRDSYRVALDGTFAPHAMPKLSGGVEGFNVDLAQLIGERLGSEMTVDAAQFS
GLIPALQAGTYDFLVAPVTVNQERSENLLFTEGFMDTNFAFVVPAGSEKRDGIEAFKGMTISVNRGSAYHTYLDDHAAEY
GWTVVPFGTNTDAVEAVATGRADANLSGATVAAWAALQNPAVDLSYEIQTGLVWGLAFRLDDAATRNRVDLVLECLKTDG
SLAALSEKWFGVTPAAGSTIVTPTPGFGVPGFPGYQEDAHQPSCDFS
>Mature_286_residues
TNPRFGGLVALAFMAATTLPAQSQVAEIRDSYRVALDGTFAPHAMPKLSGGVEGFNVDLAQLIGERLGSEMTVDAAQFSG
LIPALQAGTYDFLVAPVTVNQERSENLLFTEGFMDTNFAFVVPAGSEKRDGIEAFKGMTISVNRGSAYHTYLDDHAAEYG
WTVVPFGTNTDAVEAVATGRADANLSGATVAAWAALQNPAVDLSYEIQTGLVWGLAFRLDDAATRNRVDLVLECLKTDGS
LAALSEKWFGVTPAAGSTIVTPTPGFGVPGFPGYQEDAHQPSCDFS

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=230, Percent_Identity=23.9130434782609, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788649, Length=250, Percent_Identity=24.4, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787088, Length=228, Percent_Identity=26.7543859649123, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787085, Length=250, Percent_Identity=25.2, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787031, Length=225, Percent_Identity=27.5555555555556, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788228, Length=256, Percent_Identity=25.78125, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30355; Mature: 30224

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPRFGGLVALAFMAATTLPAQSQVAEIRDSYRVALDGTFAPHAMPKLSGGVEGFNVDL
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEEECCCCCCCCCHHHCCCCCCCCCHH
AQLIGERLGSEMTVDAAQFSGLIPALQAGTYDFLVAPVTVNQERSENLLFTEGFMDTNFA
HHHHHHHCCCCEEEEHHHHHCCCHHHHCCCEEEEEEEEEECCCCCCCEEEECCCCCCCCE
FVVPAGSEKRDGIEAFKGMTISVNRGSAYHTYLDDHAAEYGWTVVPFGTNTDAVEAVATG
EEEECCCCCCCCHHHHCCEEEEEECCCEEEEHHHHHHHHCCCEEEECCCCCHHHHHHHCC
RADANLSGATVAAWAALQNPAVDLSYEIQTGLVWGLAFRLDDAATRNRVDLVLECLKTDG
CCCCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEEEEEECCHHHHHHHHHHHHHHHCCC
SLAALSEKWFGVTPAAGSTIVTPTPGFGVPGFPGYQEDAHQPSCDFS
CEEEHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TNPRFGGLVALAFMAATTLPAQSQVAEIRDSYRVALDGTFAPHAMPKLSGGVEGFNVDL
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEEECCCCCCCCCHHHCCCCCCCCCHH
AQLIGERLGSEMTVDAAQFSGLIPALQAGTYDFLVAPVTVNQERSENLLFTEGFMDTNFA
HHHHHHHCCCCEEEEHHHHHCCCHHHHCCCEEEEEEEEEECCCCCCCEEEECCCCCCCCE
FVVPAGSEKRDGIEAFKGMTISVNRGSAYHTYLDDHAAEYGWTVVPFGTNTDAVEAVATG
EEEECCCCCCCCHHHHCCEEEEEECCCEEEEHHHHHHHHCCCEEEECCCCCHHHHHHHCC
RADANLSGATVAAWAALQNPAVDLSYEIQTGLVWGLAFRLDDAATRNRVDLVLECLKTDG
CCCCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEEEEEECCHHHHHHHHHHHHHHHCCC
SLAALSEKWFGVTPAAGSTIVTPTPGFGVPGFPGYQEDAHQPSCDFS
CEEEHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]