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The map label for this gene is dat [H]

Identifier: 77465457

GI number: 77465457

Start: 524927

End: 525784

Strand: Direct

Name: dat [H]

Synonym: RSP_3455

Alternate gene names: 77465457

Gene position: 524927-525784 (Clockwise)

Preceding gene: 77465456

Following gene: 77465458

Centisome position: 55.66

GC content: 69.81

Gene sequence:

>858_bases
ATGACCCGCACCCTCTATCTCAACGGCGCCTGGTTGCCCGAATCGGAGGCCAAGGTTTCGGTCTTCGACCGCGGCTTCCT
CATGGCGGACGGCATCTACGAGGTGACCTGCGTGCTCGACGGCAAGCTCGTCGATTATGCGGGCCATGCCGCGCGGCTGC
AGCGGTCGGCGCAGGAGCTGGGCATGGCCCTGCCGCTCACCGAAGCGGAACTGCTCGAGGTCCATCGCGAGATCGTGGCC
CGCAACGCGCTCGATCAGGGGCTGATCTACCTCCAGCTCACCCGCGGCGTGGCCGAGCGCGACTTCGTCTATCCGCCCGC
CGGCACGCCGCCGACGCTCGTGATGTTCACACAGGCCAAGAACGTGCTCGAGAATGCCGCGGCCGAGGCAGGCATCCGGG
TCGCGCTGCTGCCCGACCTGCGCTGGGGACGGCGCGACATCAAGACGGTGCAGCTCCTCTATCCCTGCATGGCGAAGATG
GAGGCCAAGGCCCGCGGCGCCGACGATGCCTGGCTCGTCGAAGACGGTTTCGTGACCGAGGGCAGCGCCGCCACCTCGCA
TATCGTGACCGCCGAGGGCGTGCTCGTCACCCGAGACCTCTCGCATGCGCTCCTGCCCGGCATCACCCGCGCCTCGGTGC
TGGAGCTGGCCGCGGCGCATGGCGTGCGGGTCGAGGAACGCGCCTTCACCCCGGACGAGGCGCGCGCCGCGCGCGAGGCC
TTCATCACCTCGGCCACCAACTTCGTGGTTCCCGTGGTGGCCATCGACGGCGCGACCGTGGGCGACGGCAAGCCCGGCGC
GCTGACCCGCGACCTGCGGCGCTTCTACATCGAGACCCGCCGCGCGTCGGCAATCTGA

Upstream 100 bases:

>100_bases
ATACCGCGGGCGCGGTCGCCATTGCCCGCCGCCTGCGCAGCGCCCTCGAAGAGAACGGCATCGCCATCGCGCCGTTCGCC
CAAGCCTGAAGGATCCGACC

Downstream 100 bases:

>100_bases
GGAGAGAGACCATGGCCGAACGGATCAACCGCGACACGCGCGGCGTCTATATCATCGCCGCGACCCCCTTCACGGATCGG
GGCGAGATCGACCACGACAG

Product: branched chain amino acid: 2-keto-4-methylthiobutyrate aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTRTLYLNGAWLPESEAKVSVFDRGFLMADGIYEVTCVLDGKLVDYAGHAARLQRSAQELGMALPLTEAELLEVHREIVA
RNALDQGLIYLQLTRGVAERDFVYPPAGTPPTLVMFTQAKNVLENAAAEAGIRVALLPDLRWGRRDIKTVQLLYPCMAKM
EAKARGADDAWLVEDGFVTEGSAATSHIVTAEGVLVTRDLSHALLPGITRASVLELAAAHGVRVEERAFTPDEARAAREA
FITSATNFVVPVVAIDGATVGDGKPGALTRDLRRFYIETRRASAI

Sequences:

>Translated_285_residues
MTRTLYLNGAWLPESEAKVSVFDRGFLMADGIYEVTCVLDGKLVDYAGHAARLQRSAQELGMALPLTEAELLEVHREIVA
RNALDQGLIYLQLTRGVAERDFVYPPAGTPPTLVMFTQAKNVLENAAAEAGIRVALLPDLRWGRRDIKTVQLLYPCMAKM
EAKARGADDAWLVEDGFVTEGSAATSHIVTAEGVLVTRDLSHALLPGITRASVLELAAAHGVRVEERAFTPDEARAAREA
FITSATNFVVPVVAIDGATVGDGKPGALTRDLRRFYIETRRASAI
>Mature_284_residues
TRTLYLNGAWLPESEAKVSVFDRGFLMADGIYEVTCVLDGKLVDYAGHAARLQRSAQELGMALPLTEAELLEVHREIVAR
NALDQGLIYLQLTRGVAERDFVYPPAGTPPTLVMFTQAKNVLENAAAEAGIRVALLPDLRWGRRDIKTVQLLYPCMAKME
AKARGADDAWLVEDGFVTEGSAATSHIVTAEGVLVTRDLSHALLPGITRASVLELAAAHGVRVEERAFTPDEARAAREAF
ITSATNFVVPVVAIDGATVGDGKPGALTRDLRRFYIETRRASAI

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=287, Percent_Identity=24.390243902439, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 30930; Mature: 30799

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTLYLNGAWLPESEAKVSVFDRGFLMADGIYEVTCVLDGKLVDYAGHAARLQRSAQEL
CCEEEEECCCCCCCCCCEEEEEECCEEEECCCEEEEEEECCEEEHHCCHHHHHHHHHHHC
GMALPLTEAELLEVHREIVARNALDQGLIYLQLTRGVAERDFVYPPAGTPPTLVMFTQAK
CCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEECCCCCCCCEEEEEHHHH
NVLENAAAEAGIRVALLPDLRWGRRDIKTVQLLYPCMAKMEAKARGADDAWLVEDGFVTE
HHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEC
GSAATSHIVTAEGVLVTRDLSHALLPGITRASVLELAAAHGVRVEERAFTPDEARAAREA
CCCCCCEEEEECCEEEEECHHHHHHCCCHHHHHHHHHHHCCCEEHHCCCCCHHHHHHHHH
FITSATNFVVPVVAIDGATVGDGKPGALTRDLRRFYIETRRASAI
HHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRTLYLNGAWLPESEAKVSVFDRGFLMADGIYEVTCVLDGKLVDYAGHAARLQRSAQEL
CEEEEECCCCCCCCCCEEEEEECCEEEECCCEEEEEEECCEEEHHCCHHHHHHHHHHHC
GMALPLTEAELLEVHREIVARNALDQGLIYLQLTRGVAERDFVYPPAGTPPTLVMFTQAK
CCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEECCCCCCCCEEEEEHHHH
NVLENAAAEAGIRVALLPDLRWGRRDIKTVQLLYPCMAKMEAKARGADDAWLVEDGFVTE
HHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEC
GSAATSHIVTAEGVLVTRDLSHALLPGITRASVLELAAAHGVRVEERAFTPDEARAAREA
CCCCCCEEEEECCEEEEECHHHHHHCCCHHHHHHHHHHHCCCEEHHCCCCCHHHHHHHHH
FITSATNFVVPVVAIDGATVGDGKPGALTRDLRRFYIETRRASAI
HHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]