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The map label for this gene is iolD [H]

Identifier: 77465289

GI number: 77465289

Start: 340942

End: 342786

Strand: Direct

Name: iolD [H]

Synonym: RSP_3284

Alternate gene names: 77465289

Gene position: 340942-342786 (Clockwise)

Preceding gene: 77465288

Following gene: 77465290

Centisome position: 36.15

GC content: 71.76

Gene sequence:

>1845_bases
ATGACAGGCACGGACCAGGACGACGCCGGGACGCTGCGGCTGACGGCGGCGCAGGCGATGGTGCGCTGGCTCTCGGTGCA
GCGGACCGAGGAGGGCGGGCGCTTCATCGAGGGCGTCTGGGCGATCTTCGGCCACGGCAATGTGGCGGGCCTGGGCGAGG
CGCTGCACGGGATCGGCGACGCGCTGCCCACCTGGCGCGGGCAGAACGAACAGACCATGGCCCATGCCGCCGTGGCCTTC
GCCAAGCAGAAGCGGCGGCGGCAGGCGATGGCCGTCACCTCCTCGATCGGGCCGGGCGCCACGAACATGGTCACGGCCGC
GGCGCTGGCCCATGTCAACCGGCTGCCCGTGCTGTTCCTTCTGGGCGACGTCTTCGCCAACCGCCGCCCCGACCCGGTGC
TCCAGCAGATCGAGGATTTCGACGACGGCACGGTGTCGGGCAACGACTGTTTCCGGCCGGTGGTGCGCTACTTCGACCGG
ATCCAGCGGCCCGAGCATCTGCTGACGGCGCTGCAGCGGGCGCTGCAGGTGATGACGGACCCGGCGAACTGCGGCCCGGT
CTGCCTCGCCTTCTGTCAGGACGTGCAGGCCGAGGCCTTCAACTATCCCGCGCGCTTCTTCGCCCCGCGCGTCTGGCGCA
TCCGGCGGCCCGACCCCGATCCGGTCGAGGTCGAAGCGGTGGCGGTGCGGCTGCGCGGGGCGCGGCGGCCGGTGATCGTG
GCCGGAGGCGGCGTGCTCTATTCCGGCGCCGAGGCGGAGCTTGCGGCCTTCTCGCAGCGCCATCGGATCCCGGTGGTCGA
GACGCAGGCCGGCAAGGGCATCCTCGACTGGCAGGAGCCGCTCAACTTCGGCTCGCCCGGCGTGACGGGATCCGAGTGCG
GCAACCGCCTCTGTGCCGAGGCCGACGTCATCCTCGGCGTGGGCACAAGGTTTCAGGATTTTACCACCGGCAGCCGGACG
ATCTTCGCCGAGGCCGACCTTCTGTCGGTCAACCTCCATCCCTACGATGCGCATAAGCACGGGGCGCTGCCGCTCGTCGC
GGATGCGAAGGCCGCGCTGGCGCGCCTGACGGAGGCGCTCGGGGACGCGCAGTGGCCCGAGCCCGACGCGGCCCTGCGGG
CGGACTGGTTTGCGGCCACCGAGGCGGCGATGGCGCGGCCCTCCGACAATGCGCTGCCCACGGACGCGCAGGTCATCGGC
GCGGTGCAGCGGGTCGCGACCGCCCGGACGGTCGTCATGGGCGCCGCGGGCACGATGCCGGGCGCGCTGCAGCTGCTCTG
GCGGGCCTCGCCCGGCGGCTATCACATGGAATACGGCTACAGCTGCATGGGCTACGAGGTGGCCGGCGCCCTCGGGATCG
CCCTGGCCGAGCCTGACCGCGAGGTCATCTGCTTTGCAGGCGACGGCAGCTACATGATGGCCAACTCGGAACTGGCGACC
GCGGTCATGCGGCGCGTGCCCTTCACGGTCGTTCTGACCGACAACCGCGGCTATGGCTGCATCAACCGCCTGCAGCAGGC
GTGCGGCGGCGCTCCGTTCAACAACCTGTATCGGGATGCGCGCGTCGAAGCCCAGCCCGAGATCGATTTCGTGGGCCATG
CCGCCGCGATGGGCGCCCGTGCGGTCAAGGCGGACGGCATTCCCGCGCTCGAGGCCGAGATCGTGGCCGCGCGCGGGCGC
GACCGGCCGACCGTGATCGTCATCGAGACCGATCCGGAACCGGAGACGGGTGTCGGCGGTCACTGGTGGGACGTGGCCGT
GCCGCAGGCGGGCGAGGGCGCGCGGCTGGCAGAGGCTCAAGCCCGCTACGCTACCCACGCCGCGCGGCAGCGCACCTTCG
ACTAG

Upstream 100 bases:

>100_bases
TCCGGAGGTCGGGCCTCTCCGAAGCCGGGATCCGGAGCGACCGGCCCGTCGGGGTGGATCCCTCCGGGACGAGGCGTGCA
GGCATGGAGGAGAAGGCGAC

Downstream 100 bases:

>100_bases
GAGAGCCCGATGATCCTCTATGGCACCAACCCCATCGCCTGGTCGAACGACGACGACCGGAGCATCGGCGCGCATCTGAG
CCTCGACGACTGCCTGTCCG

Product: acetolactate synthase

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MTGTDQDDAGTLRLTAAQAMVRWLSVQRTEEGGRFIEGVWAIFGHGNVAGLGEALHGIGDALPTWRGQNEQTMAHAAVAF
AKQKRRRQAMAVTSSIGPGATNMVTAAALAHVNRLPVLFLLGDVFANRRPDPVLQQIEDFDDGTVSGNDCFRPVVRYFDR
IQRPEHLLTALQRALQVMTDPANCGPVCLAFCQDVQAEAFNYPARFFAPRVWRIRRPDPDPVEVEAVAVRLRGARRPVIV
AGGGVLYSGAEAELAAFSQRHRIPVVETQAGKGILDWQEPLNFGSPGVTGSECGNRLCAEADVILGVGTRFQDFTTGSRT
IFAEADLLSVNLHPYDAHKHGALPLVADAKAALARLTEALGDAQWPEPDAALRADWFAATEAAMARPSDNALPTDAQVIG
AVQRVATARTVVMGAAGTMPGALQLLWRASPGGYHMEYGYSCMGYEVAGALGIALAEPDREVICFAGDGSYMMANSELAT
AVMRRVPFTVVLTDNRGYGCINRLQQACGGAPFNNLYRDARVEAQPEIDFVGHAAAMGARAVKADGIPALEAEIVAARGR
DRPTVIVIETDPEPETGVGGHWWDVAVPQAGEGARLAEAQARYATHAARQRTFD

Sequences:

>Translated_614_residues
MTGTDQDDAGTLRLTAAQAMVRWLSVQRTEEGGRFIEGVWAIFGHGNVAGLGEALHGIGDALPTWRGQNEQTMAHAAVAF
AKQKRRRQAMAVTSSIGPGATNMVTAAALAHVNRLPVLFLLGDVFANRRPDPVLQQIEDFDDGTVSGNDCFRPVVRYFDR
IQRPEHLLTALQRALQVMTDPANCGPVCLAFCQDVQAEAFNYPARFFAPRVWRIRRPDPDPVEVEAVAVRLRGARRPVIV
AGGGVLYSGAEAELAAFSQRHRIPVVETQAGKGILDWQEPLNFGSPGVTGSECGNRLCAEADVILGVGTRFQDFTTGSRT
IFAEADLLSVNLHPYDAHKHGALPLVADAKAALARLTEALGDAQWPEPDAALRADWFAATEAAMARPSDNALPTDAQVIG
AVQRVATARTVVMGAAGTMPGALQLLWRASPGGYHMEYGYSCMGYEVAGALGIALAEPDREVICFAGDGSYMMANSELAT
AVMRRVPFTVVLTDNRGYGCINRLQQACGGAPFNNLYRDARVEAQPEIDFVGHAAAMGARAVKADGIPALEAEIVAARGR
DRPTVIVIETDPEPETGVGGHWWDVAVPQAGEGARLAEAQARYATHAARQRTFD
>Mature_613_residues
TGTDQDDAGTLRLTAAQAMVRWLSVQRTEEGGRFIEGVWAIFGHGNVAGLGEALHGIGDALPTWRGQNEQTMAHAAVAFA
KQKRRRQAMAVTSSIGPGATNMVTAAALAHVNRLPVLFLLGDVFANRRPDPVLQQIEDFDDGTVSGNDCFRPVVRYFDRI
QRPEHLLTALQRALQVMTDPANCGPVCLAFCQDVQAEAFNYPARFFAPRVWRIRRPDPDPVEVEAVAVRLRGARRPVIVA
GGGVLYSGAEAELAAFSQRHRIPVVETQAGKGILDWQEPLNFGSPGVTGSECGNRLCAEADVILGVGTRFQDFTTGSRTI
FAEADLLSVNLHPYDAHKHGALPLVADAKAALARLTEALGDAQWPEPDAALRADWFAATEAAMARPSDNALPTDAQVIGA
VQRVATARTVVMGAAGTMPGALQLLWRASPGGYHMEYGYSCMGYEVAGALGIALAEPDREVICFAGDGSYMMANSELATA
VMRRVPFTVVLTDNRGYGCINRLQQACGGAPFNNLYRDARVEAQPEIDFVGHAAAMGARAVKADGIPALEAEIVAARGRD
RPTVIVIETDPEPETGVGGHWWDVAVPQAGEGARLAEAQARYATHAARQRTFD

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=485, Percent_Identity=23.2989690721649, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI87081685, Length=551, Percent_Identity=26.497277676951, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1790104, Length=500, Percent_Identity=26.4, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1786717, Length=477, Percent_Identity=26.2054507337526, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787096, Length=475, Percent_Identity=23.1578947368421, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788716, Length=500, Percent_Identity=22.2, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323755, Length=571, Percent_Identity=23.9929947460595, Blast_Score=121, Evalue=4e-28,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 65869; Mature: 65738

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTDQDDAGTLRLTAAQAMVRWLSVQRTEEGGRFIEGVWAIFGHGNVAGLGEALHGIGD
CCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHEEHHHHHCCCCHHHHHHHHHHHHH
ALPTWRGQNEQTMAHAAVAFAKQKRRRQAMAVTSSIGPGATNMVTAAALAHVNRLPVLFL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHH
LGDVFANRRPDPVLQQIEDFDDGTVSGNDCFRPVVRYFDRIQRPEHLLTALQRALQVMTD
HHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PANCGPVCLAFCQDVQAEAFNYPARFFAPRVWRIRRPDPDPVEVEAVAVRLRGARRPVIV
CCCCCHHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCCCCCCEEEEEEECCCCCCEEE
AGGGVLYSGAEAELAAFSQRHRIPVVETQAGKGILDWQEPLNFGSPGVTGSECGNRLCAE
ECCCEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCCCCCCCCHHHHHCHHHCC
ADVILGVGTRFQDFTTGSRTIFAEADLLSVNLHPYDAHKHGALPLVADAKAALARLTEAL
CCEEEECCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHH
GDAQWPEPDAALRADWFAATEAAMARPSDNALPTDAQVIGAVQRVATARTVVMGAAGTMP
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCC
GALQLLWRASPGGYHMEYGYSCMGYEVAGALGIALAEPDREVICFAGDGSYMMANSELAT
HHHHEEEECCCCCEEEECCCEEECCHHHHHHEEEEECCCCEEEEEECCCCEEEECHHHHH
AVMRRVPFTVVLTDNRGYGCINRLQQACGGAPFNNLYRDARVEAQPEIDFVGHAAAMGAR
HHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCH
AVKADGIPALEAEIVAARGRDRPTVIVIETDPEPETGVGGHWWDVAVPQAGEGARLAEAQ
HEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHH
ARYATHAARQRTFD
HHHHHHHHHHCCCC
>Mature Secondary Structure 
TGTDQDDAGTLRLTAAQAMVRWLSVQRTEEGGRFIEGVWAIFGHGNVAGLGEALHGIGD
CCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHEEHHHHHCCCCHHHHHHHHHHHHH
ALPTWRGQNEQTMAHAAVAFAKQKRRRQAMAVTSSIGPGATNMVTAAALAHVNRLPVLFL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHH
LGDVFANRRPDPVLQQIEDFDDGTVSGNDCFRPVVRYFDRIQRPEHLLTALQRALQVMTD
HHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PANCGPVCLAFCQDVQAEAFNYPARFFAPRVWRIRRPDPDPVEVEAVAVRLRGARRPVIV
CCCCCHHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCCCCCCEEEEEEECCCCCCEEE
AGGGVLYSGAEAELAAFSQRHRIPVVETQAGKGILDWQEPLNFGSPGVTGSECGNRLCAE
ECCCEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCCCCCCCCHHHHHCHHHCC
ADVILGVGTRFQDFTTGSRTIFAEADLLSVNLHPYDAHKHGALPLVADAKAALARLTEAL
CCEEEECCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHH
GDAQWPEPDAALRADWFAATEAAMARPSDNALPTDAQVIGAVQRVATARTVVMGAAGTMP
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCC
GALQLLWRASPGGYHMEYGYSCMGYEVAGALGIALAEPDREVICFAGDGSYMMANSELAT
HHHHEEEECCCCCEEEECCCEEECCHHHHHHEEEEECCCCEEEEEECCCCEEEECHHHHH
AVMRRVPFTVVLTDNRGYGCINRLQQACGGAPFNNLYRDARVEAQPEIDFVGHAAAMGAR
HHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCH
AVKADGIPALEAEIVAARGRDRPTVIVIETDPEPETGVGGHWWDVAVPQAGEGARLAEAQ
HEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHH
ARYATHAARQRTFD
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA