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The map label for this gene is bioA [C]

Identifier: 77465266

GI number: 77465266

Start: 318320

End: 319702

Strand: Direct

Name: bioA [C]

Synonym: RSP_3261

Alternate gene names: 77465266

Gene position: 318320-319702 (Clockwise)

Preceding gene: 77465254

Following gene: 77465267

Centisome position: 33.76

GC content: 68.91

Gene sequence:

>1383_bases
ATGAACAGCCTCTCGAACTCGCTCGCCGCCGCAGACATAGCCCATTCGCTGCACCCCTATACCAACGCCCGCGCACACGA
ACGGAACGGACCCCTCATCATCACGCGCGGTCAGGGCGTGCATGTCTATGACAGCGAGGGGCGGGAATATATCGAGGGTC
TGGCAGGGCTCTGGTCGGTGGCCGTGGGCTTCTCCGAGCCGCGTCTGGCCGAGGCTGCCGCGCGGCAGATGGCCGAGCTG
CCCTATTATCACACCTTCGCCGGCAAGGCGCACGAGCCGCAGATCCGGCTGGCCGAGAAGCTGGTCGAGATGACGCCCGA
GGCGCTGACGCGGGTGTTCTTCACCAACTCGGGCTCCGAGGCCAACGACACGGTGGTCAAGCTCGTCTGGTTCATGAACA
ACGCCCTCGGCCGGCCGCAGAAGAAGAAGTTCCTGTCGCGGATCAAGGGCTATCACGGCATCACCATCGCCTCGGGGTCG
CTCACGGGGCTGCCCGTCAACCACAAGGGCTTCGATCTGCCCGCCATCCCGGTCGTGCATCTGGGCTGCCCGCACCACTG
GCGCTTCGGCGCCGAGGGCGAGAGCGAGGCCGAGTTCTGCGCCCGCCTCCTGCGCGAGGCCGAGGAGACGATCCTCGCCG
AGGGGCCCGAGACCGTCGCCGCCTTCATCGGCGAGCCGGTCATGGGCGCGGGCGGGGTGATCGTGCCGCCGGACGGCTAC
TGGTCGGGGATCGAGGCGCTCTGCCGGAAATACGACATCCTTCTCGTCTCGGACGAGGTCATCAACGGCTTCGGCCGGAC
CGGCGCGCGCTGGGGATGCGAGAAGTTCGGCTTCACGCCCGACATCCTCGTGACCTCGAAGCAGCTCACCTCCTCCTATA
TGCCGCTCGCGGCGGTGCTGATGACCGACAAGGTCTATCAGACCATCGCCGACCATACGGAGACGCTGGGCACGCTCGGC
CACGGCTTCACCGGTTCGGGCCATCCGGTCGCCACGGCGGTGGGGCTCGAGAACCTGACCGTCATCGAGGAGCGCGACCT
GATGGGCAATGCGGCCCGTCTCGAGCGCCCGTTCCAGGACGGGCTGCGCGCGTTCCGGGACAATCCGCTGGTGGGCGAGG
TGCGGGGCACGGGGCTCATCGCCGCGATCGAGATGGTGGCCGACCGGGCCACGAAGCGCAGCTTCGAGCCCTTGGGCAAG
GTGGGGGCGCGCGCCGCGGCCTTCGCGGCCGAAGAAGGTCTCCTCTGCCGGGCGCTCGGCGACACGCTGGCGCTCTGCCC
GCCGCTGATCGTGACCGAGGCCGACGTGGGCCGCATCCTCGAACGGATGGGCCGTGCGCTCGACCGGACGGCGGACTGGG
CCCGGGCCGAGGGGCTGATCTGA

Upstream 100 bases:

>100_bases
AACCTGTCGTGAGCACCGGGTCCATGGCATCCGGGACAGAGATCGACAAACGGCCCGGAAACGGCTAGATGCAAAGATGC
AACCCTCGCATGGCCTTATG

Downstream 100 bases:

>100_bases
GACATGGCGGCCCGTCCCGCACCGGGAGGCCGGGCCGATTACCGGATCTTCCGCGAGCACAGCACGCGCTGGATGGACAA
TGATGCCTACGGCCATTTGA

Product: aminotransferase

Products: S-Adenosyl-4-methylthio-2-oxobutanoate; 7,8-Diaminononanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNSLSNSLAAADIAHSLHPYTNARAHERNGPLIITRGQGVHVYDSEGREYIEGLAGLWSVAVGFSEPRLAEAAARQMAEL
PYYHTFAGKAHEPQIRLAEKLVEMTPEALTRVFFTNSGSEANDTVVKLVWFMNNALGRPQKKKFLSRIKGYHGITIASGS
LTGLPVNHKGFDLPAIPVVHLGCPHHWRFGAEGESEAEFCARLLREAEETILAEGPETVAAFIGEPVMGAGGVIVPPDGY
WSGIEALCRKYDILLVSDEVINGFGRTGARWGCEKFGFTPDILVTSKQLTSSYMPLAAVLMTDKVYQTIADHTETLGTLG
HGFTGSGHPVATAVGLENLTVIEERDLMGNAARLERPFQDGLRAFRDNPLVGEVRGTGLIAAIEMVADRATKRSFEPLGK
VGARAAAFAAEEGLLCRALGDTLALCPPLIVTEADVGRILERMGRALDRTADWARAEGLI

Sequences:

>Translated_460_residues
MNSLSNSLAAADIAHSLHPYTNARAHERNGPLIITRGQGVHVYDSEGREYIEGLAGLWSVAVGFSEPRLAEAAARQMAEL
PYYHTFAGKAHEPQIRLAEKLVEMTPEALTRVFFTNSGSEANDTVVKLVWFMNNALGRPQKKKFLSRIKGYHGITIASGS
LTGLPVNHKGFDLPAIPVVHLGCPHHWRFGAEGESEAEFCARLLREAEETILAEGPETVAAFIGEPVMGAGGVIVPPDGY
WSGIEALCRKYDILLVSDEVINGFGRTGARWGCEKFGFTPDILVTSKQLTSSYMPLAAVLMTDKVYQTIADHTETLGTLG
HGFTGSGHPVATAVGLENLTVIEERDLMGNAARLERPFQDGLRAFRDNPLVGEVRGTGLIAAIEMVADRATKRSFEPLGK
VGARAAAFAAEEGLLCRALGDTLALCPPLIVTEADVGRILERMGRALDRTADWARAEGLI
>Mature_460_residues
MNSLSNSLAAADIAHSLHPYTNARAHERNGPLIITRGQGVHVYDSEGREYIEGLAGLWSVAVGFSEPRLAEAAARQMAEL
PYYHTFAGKAHEPQIRLAEKLVEMTPEALTRVFFTNSGSEANDTVVKLVWFMNNALGRPQKKKFLSRIKGYHGITIASGS
LTGLPVNHKGFDLPAIPVVHLGCPHHWRFGAEGESEAEFCARLLREAEETILAEGPETVAAFIGEPVMGAGGVIVPPDGY
WSGIEALCRKYDILLVSDEVINGFGRTGARWGCEKFGFTPDILVTSKQLTSSYMPLAAVLMTDKVYQTIADHTETLGTLG
HGFTGSGHPVATAVGLENLTVIEERDLMGNAARLERPFQDGLRAFRDNPLVGEVRGTGLIAAIEMVADRATKRSFEPLGK
VGARAAAFAAEEGLLCRALGDTLALCPPLIVTEADVGRILERMGRALDRTADWARAEGLI

Specific function: Biotin biosynthesis. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=446, Percent_Identity=28.0269058295964, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI37574042, Length=428, Percent_Identity=28.7383177570093, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI226442705, Length=428, Percent_Identity=28.7383177570093, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI24119277, Length=439, Percent_Identity=27.5626423690205, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI4557809, Length=442, Percent_Identity=26.9230769230769, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI226442709, Length=397, Percent_Identity=28.9672544080605, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI284507298, Length=335, Percent_Identity=28.0597014925373, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI188536080, Length=386, Percent_Identity=23.0569948186528, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI38679950, Length=386, Percent_Identity=23.0569948186528, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI38679946, Length=386, Percent_Identity=23.0569948186528, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1786991, Length=445, Percent_Identity=31.0112359550562, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1788044, Length=420, Percent_Identity=30.2380952380952, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI145693181, Length=357, Percent_Identity=32.4929971988796, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789016, Length=427, Percent_Identity=29.03981264637, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789759, Length=423, Percent_Identity=28.3687943262411, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787560, Length=411, Percent_Identity=27.9805352798054, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1786349, Length=324, Percent_Identity=24.6913580246914, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32564660, Length=451, Percent_Identity=29.0465631929047, Blast_Score=151, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI25144271, Length=414, Percent_Identity=26.5700483091787, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71992977, Length=443, Percent_Identity=24.6049661399549, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25144274, Length=234, Percent_Identity=30.7692307692308, Blast_Score=111, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323470, Length=450, Percent_Identity=29.3333333333333, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324386, Length=473, Percent_Identity=26.8498942917548, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324432, Length=434, Percent_Identity=25.5760368663594, Blast_Score=113, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6321456, Length=290, Percent_Identity=24.1379310344828, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28574759, Length=458, Percent_Identity=31.0043668122271, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI161085790, Length=455, Percent_Identity=31.4285714285714, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI21356575, Length=374, Percent_Identity=31.283422459893, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI21357415, Length=432, Percent_Identity=28.2407407407407, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI281366494, Length=444, Percent_Identity=22.7477477477477, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667139, Length=444, Percent_Identity=22.7477477477477, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667143, Length=444, Percent_Identity=22.7477477477477, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62 [C]

Molecular weight: Translated: 49682; Mature: 49682

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLSNSLAAADIAHSLHPYTNARAHERNGPLIITRGQGVHVYDSEGREYIEGLAGLWSV
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHH
AVGFSEPRLAEAAARQMAELPYYHTFAGKAHEPQIRLAEKLVEMTPEALTRVFFTNSGSE
HHCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCC
ANDTVVKLVWFMNNALGRPQKKKFLSRIKGYHGITIASGSLTGLPVNHKGFDLPAIPVVH
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCEECCCCCCCCCCCCCCCEEE
LGCPHHWRFGAEGESEAEFCARLLREAEETILAEGPETVAAFIGEPVMGAGGVIVPPDGY
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEECCCCC
WSGIEALCRKYDILLVSDEVINGFGRTGARWGCEKFGFTPDILVTSKQLTSSYMPLAAVL
HHHHHHHHHHCCEEEEEHHHHHHCCCCCCCCCHHHCCCCCCCEEEHHHHHHHHHHHHHHH
MTDKVYQTIADHTETLGTLGHGFTGSGHPVATAVGLENLTVIEERDLMGNAARLERPFQD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEHHHHCCCHHHHCCCHHH
GLRAFRDNPLVGEVRGTGLIAAIEMVADRATKRSFEPLGKVGARAAAFAAEEGLLCRALG
HHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
DTLALCPPLIVTEADVGRILERMGRALDRTADWARAEGLI
CHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSLSNSLAAADIAHSLHPYTNARAHERNGPLIITRGQGVHVYDSEGREYIEGLAGLWSV
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHH
AVGFSEPRLAEAAARQMAELPYYHTFAGKAHEPQIRLAEKLVEMTPEALTRVFFTNSGSE
HHCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCC
ANDTVVKLVWFMNNALGRPQKKKFLSRIKGYHGITIASGSLTGLPVNHKGFDLPAIPVVH
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCEECCCCCCCCCCCCCCCEEE
LGCPHHWRFGAEGESEAEFCARLLREAEETILAEGPETVAAFIGEPVMGAGGVIVPPDGY
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEECCCCC
WSGIEALCRKYDILLVSDEVINGFGRTGARWGCEKFGFTPDILVTSKQLTSSYMPLAAVL
HHHHHHHHHHCCEEEEEHHHHHHCCCCCCCCCHHHCCCCCCCEEEHHHHHHHHHHHHHHH
MTDKVYQTIADHTETLGTLGHGFTGSGHPVATAVGLENLTVIEERDLMGNAARLERPFQD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEHHHHCCCHHHHCCCHHH
GLRAFRDNPLVGEVRGTGLIAAIEMVADRATKRSFEPLGKVGARAAAFAAEEGLLCRALG
HHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
DTLALCPPLIVTEADVGRILERMGRALDRTADWARAEGLI
CHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Amino-7-oxononanoate; S-Adenosyl-L-methionine [C]

Specific reaction: 8-Amino-7-oxononanoate + S-Adenosyl-L-methionine <==> S-Adenosyl-4-methylthio-2-oxobutanoate + 7,8-Diaminononanoate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]