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The map label for this gene is lguL [H]

Identifier: 77465248

GI number: 77465248

Start: 294958

End: 295353

Strand: Direct

Name: lguL [H]

Synonym: RSP_3237

Alternate gene names: 77465248

Gene position: 294958-295353 (Clockwise)

Preceding gene: 77465247

Following gene: 77465249

Centisome position: 31.28

GC content: 63.13

Gene sequence:

>396_bases
TTGGCCAAGATGATCCACAGCATGATCCGCGTGCAGGACGAGGCGCGCTCGCTTGCCTTCTACCGCGAGGCCTTCGGCCT
CGAGGTGGCCGAGCGGCTCGATTTCGAGAGTTTCACGCTGCTCTATCTCTCGAACGCCGAAAGCGAGTTCGAGCTGGAGC
TCACCGTCAACAAGGGCCGCGCGGAGCCCTATGCCCTTGGCGACGGTTACGGCCATCTGGCGGTCTCGGTCGCAGATGTC
GCAGCCGAGCATGCACGGCTGACGGAGGCGGGCCTCAACCCCCGGAAAATAGTGGATTTTGCCCCCGGCGGCGAGGTGAT
CGCCCGCTTCTTCTTCATCGCGGATCCCGACGGATACCAGATTGAAGTCTTGGAGCGCGGCGGCCGCTACAAGTAA

Upstream 100 bases:

>100_bases
TGACGCCTCCGCGCGGCCCGTCCGGCCGAGGGAGGCGACCTCCGGCGGCAGCGCTGCCGCCCCTCCCTTTCCCTGCGGCA
CCTGTTCAGGAGGACCACCC

Downstream 100 bases:

>100_bases
GCTTCCGCCCGGTGCCGGCGCCCGGGTCGATCGGAGCGCTCCGGTCTCAGTTGGAAGCAAGGGGGTATCATGCCGAAGAG
CATGGGAATGGCCGTCGCCG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MAKMIHSMIRVQDEARSLAFYREAFGLEVAERLDFESFTLLYLSNAESEFELELTVNKGRAEPYALGDGYGHLAVSVADV
AAEHARLTEAGLNPRKIVDFAPGGEVIARFFFIADPDGYQIEVLERGGRYK

Sequences:

>Translated_131_residues
MAKMIHSMIRVQDEARSLAFYREAFGLEVAERLDFESFTLLYLSNAESEFELELTVNKGRAEPYALGDGYGHLAVSVADV
AAEHARLTEAGLNPRKIVDFAPGGEVIARFFFIADPDGYQIEVLERGGRYK
>Mature_130_residues
AKMIHSMIRVQDEARSLAFYREAFGLEVAERLDFESFTLLYLSNAESEFELELTVNKGRAEPYALGDGYGHLAVSVADVA
AEHARLTEAGLNPRKIVDFAPGGEVIARFFFIADPDGYQIEVLERGGRYK

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Escherichia coli, GI1787940, Length=127, Percent_Identity=33.0708661417323, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14635; Mature: 14503

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMIHSMIRVQDEARSLAFYREAFGLEVAERLDFESFTLLYLSNAESEFELELTVNKGR
CHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEEECCCCEEEEEEEEECCC
AEPYALGDGYGHLAVSVADVAAEHARLTEAGLNPRKIVDFAPGGEVIARFFFIADPDGYQ
CCCEEECCCCCEEEEHHHHHHHHHHHHHHCCCCCHHEEEECCCHHHEEEEEEEECCCCCE
IEVLERGGRYK
EEEEECCCCCC
>Mature Secondary Structure 
AKMIHSMIRVQDEARSLAFYREAFGLEVAERLDFESFTLLYLSNAESEFELELTVNKGR
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEEECCCCEEEEEEEEECCC
AEPYALGDGYGHLAVSVADVAAEHARLTEAGLNPRKIVDFAPGGEVIARFFFIADPDGYQ
CCCEEECCCCCEEEEHHHHHHHHHHHHHHCCCCCHHEEEECCCHHHEEEEEEEECCCCCE
IEVLERGGRYK
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 9719565 [H]