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The map label for this gene is rnfE [H]

Identifier: 77465211

GI number: 77465211

Start: 250706

End: 251440

Strand: Direct

Name: rnfE [H]

Synonym: RSP_3197

Alternate gene names: 77465211

Gene position: 250706-251440 (Clockwise)

Preceding gene: 77465210

Following gene: 77465212

Centisome position: 26.59

GC content: 68.16

Gene sequence:

>735_bases
ATGTCCACGGACTTCGGCAAGATCGCCCGCGACGGGCTCTGGGACAAGAACATCGTGGTGGGCCAGATGCTGGCGCTCTG
TCCCACGCTCGCCATCACCGGCACGGCCACCAATGGGCTCGGGATGGGGCTGGCCACCACCGCCGTCCTCATCCTGTCGA
ACGCGGCCATCTCGGCCCTGCGCCGCGCCATCACGCCCGAGATCCGCATCCCGGCCTTCGTCCTCGTGATCGCGGCCATC
GTGACGGTGGTCGATCTCGCGCTGAATGCCTGGGTCCACGACCTGCACAAGGTTCTGGGCCTCTTCATCGCGCTGATCGT
CACGAACTGCGCCATTCTCGGGCGCGCCGAAGCCTTCGCCTCGCGCCACGGCATCCTCGCTTCGGCCTTCGACGGGCTGA
CCATGGGCGTGGGCTTCACGCTGGCGCTGGTCCTCGTCGGGGCGGTGCGCGAGCTGGTGGGGTCTGGCACGCTCTTCGCG
CAGGCCTCGGTGCTGCTCGGGCCGCATTTCGCCTTCATGGAGCTCACGATCCTGCCCGACTATCCGGGCTTCCTGATCGC
CATCCTGCCGCCGGGCGGCTTCATCCTGCTCGGCGTCATCCTCGCGCTGAAGTCCATCGTCGACAGCCGCAAGAAGAGCC
TCGAGCAGGAGATCCGGGATCTGCGCGTCGAACGCGTGTTCACCGCGGCCGGCGTGCTGACGCCGCGGCTGCAAGAGGCG
GGGGACGACGCATGA

Upstream 100 bases:

>100_bases
GGTGGTGGGCACCATCCACCGCGGCCTCACCCTGTTCGACCGTCACCGCGCGGAGCTGCTCGCCCCGCTCCCACCCCGCT
CCTGAGGGAGGAGGCCCCCC

Downstream 100 bases:

>100_bases
TCGTGGGAGTGGCCTATGCCAAGCCGACCGTGCAGGTCTGGAAACATGTGGACGTGCCCGAGGGCACCTCTGCGCGCGAG
GCGATCGAACGGTCCGGGCT

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: Nitrogen fixation protein rnfE [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MSTDFGKIARDGLWDKNIVVGQMLALCPTLAITGTATNGLGMGLATTAVLILSNAAISALRRAITPEIRIPAFVLVIAAI
VTVVDLALNAWVHDLHKVLGLFIALIVTNCAILGRAEAFASRHGILASAFDGLTMGVGFTLALVLVGAVRELVGSGTLFA
QASVLLGPHFAFMELTILPDYPGFLIAILPPGGFILLGVILALKSIVDSRKKSLEQEIRDLRVERVFTAAGVLTPRLQEA
GDDA

Sequences:

>Translated_244_residues
MSTDFGKIARDGLWDKNIVVGQMLALCPTLAITGTATNGLGMGLATTAVLILSNAAISALRRAITPEIRIPAFVLVIAAI
VTVVDLALNAWVHDLHKVLGLFIALIVTNCAILGRAEAFASRHGILASAFDGLTMGVGFTLALVLVGAVRELVGSGTLFA
QASVLLGPHFAFMELTILPDYPGFLIAILPPGGFILLGVILALKSIVDSRKKSLEQEIRDLRVERVFTAAGVLTPRLQEA
GDDA
>Mature_243_residues
STDFGKIARDGLWDKNIVVGQMLALCPTLAITGTATNGLGMGLATTAVLILSNAAISALRRAITPEIRIPAFVLVIAAIV
TVVDLALNAWVHDLHKVLGLFIALIVTNCAILGRAEAFASRHGILASAFDGLTMGVGFTLALVLVGAVRELVGSGTLFAQ
ASVLLGPHFAFMELTILPDYPGFLIAILPPGGFILLGVILALKSIVDSRKKSLEQEIRDLRVERVFTAAGVLTPRLQEAG
DDA

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787919, Length=203, Percent_Identity=49.2610837438424, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1787914, Length=142, Percent_Identity=36.6197183098592, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR010968 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 25634; Mature: 25503

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDFGKIARDGLWDKNIVVGQMLALCPTLAITGTATNGLGMGLATTAVLILSNAAISAL
CCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RRAITPEIRIPAFVLVIAAIVTVVDLALNAWVHDLHKVLGLFIALIVTNCAILGRAEAFA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRHGILASAFDGLTMGVGFTLALVLVGAVRELVGSGTLFAQASVLLGPHFAFMELTILPD
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHEEEEECCC
YPGFLIAILPPGGFILLGVILALKSIVDSRKKSLEQEIRDLRVERVFTAAGVLTPRLQEA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHC
GDDA
CCCC
>Mature Secondary Structure 
STDFGKIARDGLWDKNIVVGQMLALCPTLAITGTATNGLGMGLATTAVLILSNAAISAL
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RRAITPEIRIPAFVLVIAAIVTVVDLALNAWVHDLHKVLGLFIALIVTNCAILGRAEAFA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRHGILASAFDGLTMGVGFTLALVLVGAVRELVGSGTLFAQASVLLGPHFAFMELTILPD
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHEEEEECCC
YPGFLIAILPPGGFILLGVILALKSIVDSRKKSLEQEIRDLRVERVFTAAGVLTPRLQEA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHC
GDDA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268 [H]