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The map label for this gene is pucI [H]

Identifier: 77465187

GI number: 77465187

Start: 226359

End: 227858

Strand: Reverse

Name: pucI [H]

Synonym: RSP_3174

Alternate gene names: 77465187

Gene position: 227858-226359 (Counterclockwise)

Preceding gene: 77465188

Following gene: 77465182

Centisome position: 24.16

GC content: 68.0

Gene sequence:

>1500_bases
ATGACACTGGCCGACCAGCTGCGACGCGCCGAGCACGACCGCTCGGCCCTGATCGAGGAATCGATCCTGCCCACCCACCT
CAACCAGCGTCCCATCGGGATGATCGGCTATGGCTGGATCTGGGTCGGCATCGCCGTCATCATCGCCACCTACTCGCTGG
GCGCCGCGGGCGTGGGCGGAGGCGTGCCGCTTGCCACGGTGATCCTCACCATCTTTGCGGCCAACCTCACCATCGGCGCC
TTCATGCTGCTCACGGCCGACATCGGCACCGAGCATGGCGTGCCCTTCGCGGTCTATCTGCGGGCGCCCTTCGGCATCCA
CGGCACCCACCTGCCCTCGCTCTCGCGCGGGCTGGTGGCGGCGATGTGGTTCGGCATCCAGACCTATCTCGGTGCGCTGG
CCCTGAACGGGATCGGCGAATATGTCCTCGGCGTCTCGAACTGGTTCGTCTGGTATCTCCTCTTCGGCCTCCTCCAGATC
GCGAGCACCATGGCGGGCATCCGCTCGGTCGAGCGGCTGGCGGCGCTGGCCGCGCCCGCGATCATCGCCATCTCGGTCTG
GATGTATTTCAGCCTCGAGGGGATCGCCGAGACCAAGGGGCTCAACATCTGGACCTTCCGCGCCGAGGGGCAGATGTCGC
TGCTCGCGCTCTTCATCGCCAACCTCGGCTTCTGGTCGACCATGGCCATCGACATTCCGAACCTGACCCGCTTCATCGCG
GTGAAGCCCGGCGCGCGCGGCTTCCTCAGCCGCAACCGCGCGGTGTTCCTGGGCCAGCTCGTGGCGCTGCCCGTCACTCA
GGCGCTGGTCGCGGGCATCGGCGGGGTCTCGTTCATCGCCACCGGCAACTGGAACCCGATCGAGGTGATCCAGGGGGATG
CGCAGGGCCTCTCGCTCCTCACGCTTCTCCTCCTCGTCGTGCTGGCGCAATGGTCGACGAACAACTCGGCCAACCTGATC
CCGGCCGCACTGACCTTCGTCAACCTCGCGCCGCGCCGGATCGATTACCGCATCGGCGTAGCGCTGGCGGGGGTCGTGGG
CACGCTCTGCTTCCCGTGGGAGATCCTGAACAACCTCTTCACCTTCCTCGGCTACTGCGGCGCCTTCCTGCTTTCCATCG
GCGGGATCATGGTGGCGGATTACTATGTGCTGCGCGGCCGGCGGGTGAACGTGCCTGCCCTCTACGATCCGCAGGGCCAG
TACCGCTACGCGGGCGGCTTCAATCCCGCGGGCCTCGTGGCCTGGATCGTCGCGGGGGCGGCGGCAGCCTGGTGGTCGGA
CTATTCCGTCTTCGTGGGCTTCCCGCTGGGCGCCCTCCTTTATCTCGCGCTGATGAAGCTCGTGGTGCTGCCGCGCCATC
CGCAGCCCGAGATGGCCGCGGCCGAGGGCTATCTCGCCACCTCCGAGGGGGTGAGCTGGGCCTATCTCGGCGGCGGCCGG
TTCACCCGCCTGCGCCCCGGCGAGACGGCGGGCGCGGTCGTCCCGCGCGAGGATCTGTAA

Upstream 100 bases:

>100_bases
GCCGCGCGCCGGACCCCGATGCGCAGAGCCGCGCGGCGGCGCCCGGTTCCTTCCCGACAGGCGGCGGACCGGCCCGCCGG
CCGTCCAACGGGGAGAAAAA

Downstream 100 bases:

>100_bases
CGCGCGCCGGGGGCGGGTCCCCTCACCCGTCCCCCGCGATCATCGCCACGAGGAACCGCGCGCAGACAAGGAGCAGGAAG
CAGCCGAAGCTGCGCGCTAG

Product: NCS1 nucleoside transporter

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MTLADQLRRAEHDRSALIEESILPTHLNQRPIGMIGYGWIWVGIAVIIATYSLGAAGVGGGVPLATVILTIFAANLTIGA
FMLLTADIGTEHGVPFAVYLRAPFGIHGTHLPSLSRGLVAAMWFGIQTYLGALALNGIGEYVLGVSNWFVWYLLFGLLQI
ASTMAGIRSVERLAALAAPAIIAISVWMYFSLEGIAETKGLNIWTFRAEGQMSLLALFIANLGFWSTMAIDIPNLTRFIA
VKPGARGFLSRNRAVFLGQLVALPVTQALVAGIGGVSFIATGNWNPIEVIQGDAQGLSLLTLLLLVVLAQWSTNNSANLI
PAALTFVNLAPRRIDYRIGVALAGVVGTLCFPWEILNNLFTFLGYCGAFLLSIGGIMVADYYVLRGRRVNVPALYDPQGQ
YRYAGGFNPAGLVAWIVAGAAAAWWSDYSVFVGFPLGALLYLALMKLVVLPRHPQPEMAAAEGYLATSEGVSWAYLGGGR
FTRLRPGETAGAVVPREDL

Sequences:

>Translated_499_residues
MTLADQLRRAEHDRSALIEESILPTHLNQRPIGMIGYGWIWVGIAVIIATYSLGAAGVGGGVPLATVILTIFAANLTIGA
FMLLTADIGTEHGVPFAVYLRAPFGIHGTHLPSLSRGLVAAMWFGIQTYLGALALNGIGEYVLGVSNWFVWYLLFGLLQI
ASTMAGIRSVERLAALAAPAIIAISVWMYFSLEGIAETKGLNIWTFRAEGQMSLLALFIANLGFWSTMAIDIPNLTRFIA
VKPGARGFLSRNRAVFLGQLVALPVTQALVAGIGGVSFIATGNWNPIEVIQGDAQGLSLLTLLLLVVLAQWSTNNSANLI
PAALTFVNLAPRRIDYRIGVALAGVVGTLCFPWEILNNLFTFLGYCGAFLLSIGGIMVADYYVLRGRRVNVPALYDPQGQ
YRYAGGFNPAGLVAWIVAGAAAAWWSDYSVFVGFPLGALLYLALMKLVVLPRHPQPEMAAAEGYLATSEGVSWAYLGGGR
FTRLRPGETAGAVVPREDL
>Mature_498_residues
TLADQLRRAEHDRSALIEESILPTHLNQRPIGMIGYGWIWVGIAVIIATYSLGAAGVGGGVPLATVILTIFAANLTIGAF
MLLTADIGTEHGVPFAVYLRAPFGIHGTHLPSLSRGLVAAMWFGIQTYLGALALNGIGEYVLGVSNWFVWYLLFGLLQIA
STMAGIRSVERLAALAAPAIIAISVWMYFSLEGIAETKGLNIWTFRAEGQMSLLALFIANLGFWSTMAIDIPNLTRFIAV
KPGARGFLSRNRAVFLGQLVALPVTQALVAGIGGVSFIATGNWNPIEVIQGDAQGLSLLTLLLLVVLAQWSTNNSANLIP
AALTFVNLAPRRIDYRIGVALAGVVGTLCFPWEILNNLFTFLGYCGAFLLSIGGIMVADYYVLRGRRVNVPALYDPQGQY
RYAGGFNPAGLVAWIVAGAAAAWWSDYSVFVGFPLGALLYLALMKLVVLPRHPQPEMAAAEGYLATSEGVSWAYLGGGRF
TRLRPGETAGAVVPREDL

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=452, Percent_Identity=28.7610619469027, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322219, Length=529, Percent_Identity=23.8185255198488, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319495, Length=432, Percent_Identity=21.7592592592593, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319429, Length=503, Percent_Identity=21.8687872763419, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53460; Mature: 53329

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLADQLRRAEHDRSALIEESILPTHLNQRPIGMIGYGWIWVGIAVIIATYSLGAAGVGG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCC
GVPLATVILTIFAANLTIGAFMLLTADIGTEHGVPFAVYLRAPFGIHGTHLPSLSRGLVA
CHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHH
AMWFGIQTYLGALALNGIGEYVLGVSNWFVWYLLFGLLQIASTMAGIRSVERLAALAAPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIAISVWMYFSLEGIAETKGLNIWTFRAEGQMSLLALFIANLGFWSTMAIDIPNLTRFIA
HHHHHHHHHHHHCCHHHCCCCEEEEEECCCCHHHHHHHHHHCCCHHEEEEECCCCEEEEE
VKPGARGFLSRNRAVFLGQLVALPVTQALVAGIGGVSFIATGNWNPIEVIQGDAQGLSLL
ECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCHHHHH
TLLLLVVLAQWSTNNSANLIPAALTFVNLAPRRIDYRIGVALAGVVGTLCFPWEILNNLF
HHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFLGYCGAFLLSIGGIMVADYYVLRGRRVNVPALYDPQGQYRYAGGFNPAGLVAWIVAGA
HHHHHHHHHHHHHCHHHHHHHHHHCCCEECCCEEECCCCCEEECCCCCHHHHHHHHHHHH
AAAWWSDYSVFVGFPLGALLYLALMKLVVLPRHPQPEMAAAEGYLATSEGVSWAYLGGGR
HHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCEEEEECCCE
FTRLRPGETAGAVVPREDL
EEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLADQLRRAEHDRSALIEESILPTHLNQRPIGMIGYGWIWVGIAVIIATYSLGAAGVGG
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCC
GVPLATVILTIFAANLTIGAFMLLTADIGTEHGVPFAVYLRAPFGIHGTHLPSLSRGLVA
CHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHH
AMWFGIQTYLGALALNGIGEYVLGVSNWFVWYLLFGLLQIASTMAGIRSVERLAALAAPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIAISVWMYFSLEGIAETKGLNIWTFRAEGQMSLLALFIANLGFWSTMAIDIPNLTRFIA
HHHHHHHHHHHHCCHHHCCCCEEEEEECCCCHHHHHHHHHHCCCHHEEEEECCCCEEEEE
VKPGARGFLSRNRAVFLGQLVALPVTQALVAGIGGVSFIATGNWNPIEVIQGDAQGLSLL
ECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCHHHHH
TLLLLVVLAQWSTNNSANLIPAALTFVNLAPRRIDYRIGVALAGVVGTLCFPWEILNNLF
HHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFLGYCGAFLLSIGGIMVADYYVLRGRRVNVPALYDPQGQYRYAGGFNPAGLVAWIVAGA
HHHHHHHHHHHHHCHHHHHHHHHHCCCEECCCEEECCCCCEEECCCCCHHHHHHHHHHHH
AAAWWSDYSVFVGFPLGALLYLALMKLVVLPRHPQPEMAAAEGYLATSEGVSWAYLGGGR
HHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCEEEEECCCE
FTRLRPGETAGAVVPREDL
EEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]