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The map label for this gene is yfjR [H]

Identifier: 77465185

GI number: 77465185

Start: 224637

End: 225503

Strand: Direct

Name: yfjR [H]

Synonym: RSP_3171

Alternate gene names: 77465185

Gene position: 224637-225503 (Clockwise)

Preceding gene: 77465184

Following gene: 77465186

Centisome position: 23.82

GC content: 74.63

Gene sequence:

>867_bases
ATGAGCGACACCGTGGTGGGCATTGCCGGAACCGGGCGCATGGGCACGGCCTTCGCCCGCCGCCTCCGCGAGACGGGGAC
CCCGGTCCGGGTCTGGAACCGCAGTTCCGACCGGACGGGCGCCGCGGTCGATGCCGGCGCCGAGGCCGTGGCGCTCGAAG
CGCTGGCCGAGGCCGATCTCGTTCTTCTGTCGCTGACCGATGCCGCCGCAGCCGAGGCGGTGCTGGCGGGCATGGGCGCG
GCGCTGGCGGGCCGCATCGTGGTCGAGATGTCGACGCTCCTGCCCGATCAGGCCGAGGCGCTCGAGGCGCAGGCCACGGC
GCTCGGGGCGCAGTTCCTCCATTGCCCGGTGGGCGGCACCGTGGCCCCCGCGCTGAAGGGCCAGCTTCTGGGCTTCGCGG
GCGGGCCGGCCGAGACCCTCGAACAGGCCCGGCCGGTGCTCGAACGGCTCTGCCGTCGCGTCGAGCATCTGGGGTCCGTC
GGGGCCGCGGCCCGGATGAAGCTCGCCGTGAACCTGCCGCTCGCGCTCTACTGGCAGACGCTGGGCGAGAGCCTCCTCCT
CCTGCGCGGGGCGGGCATTCCGGCCGAGCAGGCGATCGGCCTGATGGCCGAAAGCTCGGGCGGTCCCGCGGTGCTGAAGA
ACCGCGCGCAGGTGGTGGTGGAGACCCTGGAAGGGGCCGATCAGCGCGGCACCTTCGACATCGCGGGCCTCGCCAAGGAT
CTGCATCTGGCTCTGGCCCTTGCCGAGCGCGAGGGGGCGGCGCTGCCGCTCTCCGCCGCCGCCGAGGAGCGTTATCGGGC
GGCGCTGGAGGCGGGCCTCGGCCGGTTCGACGGCGCAAGCCTCGCGCGGCGGACGGCCGAAGGCTGA

Upstream 100 bases:

>100_bases
CGCGGGCCGCGCGCGGGCCGGCCGCGAGGAAGAGATGAAGCGCCGGATCGCCGCGGGCGAGACGACGCTCGCGATACTCG
GACTGATGAAGGAGGCTTGA

Downstream 100 bases:

>100_bases
TGGAGCTCCTTTCCTCGGGCACGATCTTCGCGCTCATCGCGGCGCTGGCCGCGGCGGGTGCGGTGACGGGGTTCCTCGCG
GGCCTGTTCGGGATCGGCGG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSDTVVGIAGTGRMGTAFARRLRETGTPVRVWNRSSDRTGAAVDAGAEAVALEALAEADLVLLSLTDAAAAEAVLAGMGA
ALAGRIVVEMSTLLPDQAEALEAQATALGAQFLHCPVGGTVAPALKGQLLGFAGGPAETLEQARPVLERLCRRVEHLGSV
GAAARMKLAVNLPLALYWQTLGESLLLLRGAGIPAEQAIGLMAESSGGPAVLKNRAQVVVETLEGADQRGTFDIAGLAKD
LHLALALAEREGAALPLSAAAEERYRAALEAGLGRFDGASLARRTAEG

Sequences:

>Translated_288_residues
MSDTVVGIAGTGRMGTAFARRLRETGTPVRVWNRSSDRTGAAVDAGAEAVALEALAEADLVLLSLTDAAAAEAVLAGMGA
ALAGRIVVEMSTLLPDQAEALEAQATALGAQFLHCPVGGTVAPALKGQLLGFAGGPAETLEQARPVLERLCRRVEHLGSV
GAAARMKLAVNLPLALYWQTLGESLLLLRGAGIPAEQAIGLMAESSGGPAVLKNRAQVVVETLEGADQRGTFDIAGLAKD
LHLALALAEREGAALPLSAAAEERYRAALEAGLGRFDGASLARRTAEG
>Mature_287_residues
SDTVVGIAGTGRMGTAFARRLRETGTPVRVWNRSSDRTGAAVDAGAEAVALEALAEADLVLLSLTDAAAAEAVLAGMGAA
LAGRIVVEMSTLLPDQAEALEAQATALGAQFLHCPVGGTVAPALKGQLLGFAGGPAETLEQARPVLERLCRRVEHLGSVG
AAARMKLAVNLPLALYWQTLGESLLLLRGAGIPAEQAIGLMAESSGGPAVLKNRAQVVVETLEGADQRGTFDIAGLAKDL
HLALALAEREGAALPLSAAAEERYRAALEAGLGRFDGASLARRTAEG

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=273, Percent_Identity=26.3736263736264, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI23308751, Length=289, Percent_Identity=26.9896193771626, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1790315, Length=283, Percent_Identity=30.7420494699647, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI145693186, Length=283, Percent_Identity=28.2685512367491, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=26.643598615917, Blast_Score=74, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28574115, Length=295, Percent_Identity=23.0508474576271, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24655230, Length=279, Percent_Identity=26.8817204301075, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922568, Length=279, Percent_Identity=26.8817204301075, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 29430; Mature: 29299

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTVVGIAGTGRMGTAFARRLRETGTPVRVWNRSSDRTGAAVDAGAEAVALEALAEADL
CCCCEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCHHHHHHHHHHHCCE
VLLSLTDAAAAEAVLAGMGAALAGRIVVEMSTLLPDQAEALEAQATALGAQFLHCPVGGT
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCC
VAPALKGQLLGFAGGPAETLEQARPVLERLCRRVEHLGSVGAAARMKLAVNLPLALYWQT
CCHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEECCCHHHHHHH
LGESLLLLRGAGIPAEQAIGLMAESSGGPAVLKNRAQVVVETLEGADQRGTFDIAGLAKD
HHHHEEEHCCCCCCHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LHLALALAEREGAALPLSAAAEERYRAALEAGLGRFDGASLARRTAEG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
SDTVVGIAGTGRMGTAFARRLRETGTPVRVWNRSSDRTGAAVDAGAEAVALEALAEADL
CCCEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCHHHHHHHHHHHCCE
VLLSLTDAAAAEAVLAGMGAALAGRIVVEMSTLLPDQAEALEAQATALGAQFLHCPVGGT
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCC
VAPALKGQLLGFAGGPAETLEQARPVLERLCRRVEHLGSVGAAARMKLAVNLPLALYWQT
CCHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEECCCHHHHHHH
LGESLLLLRGAGIPAEQAIGLMAESSGGPAVLKNRAQVVVETLEGADQRGTFDIAGLAKD
HHHHEEEHCCCCCCHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LHLALALAEREGAALPLSAAAEERYRAALEAGLGRFDGASLARRTAEG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]