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The map label for this gene is proW [H]

Identifier: 77465073

GI number: 77465073

Start: 98492

End: 99403

Strand: Reverse

Name: proW [H]

Synonym: RSP_3058

Alternate gene names: 77465073

Gene position: 99403-98492 (Counterclockwise)

Preceding gene: 77465074

Following gene: 77465072

Centisome position: 10.54

GC content: 66.89

Gene sequence:

>912_bases
ATGGCCTCCGCCTGCTGGAGCTTGCCCGAAGCCTTCTGCCAGTTTCCCGCGCTGGACGGCAGAACCCTGCGCATGATGCG
CAAGACCATCGACGGAAGCTTCCGCGACCTCGTGCGCAGCTTCTCCGACGCGATCGACACGGCCACCGCGCCGCTTCAGC
TGGCGCTGAACGCCATCGAGCGCGCCTTCACCTCCACCCCCTGGATCGTCGTGCTCGCGGTGCTCCTCGCCATCGTCTGG
GCCGGATCGCGCAGCTGGCGGATCGTCGCGGGCTCGGCGGTGGCCATGTGCGCCATCGGCTGGTTCGGCCTGTGGGAGGA
CACGATGGTGACGCTCGCCATGGTGTCGGTCTGCACCGTCGCCGCCGTCATCATCGGCCTGCCCATCGGCATCCTCGCGG
CCCGCTCCGACCGGATGCAGCGCCTCGTCAATCCCGTGCTCGACGTGATGCAGACGCTGCCCTCCTTCGTCTATCTCATT
CCCGTCGTGGTGATCTTCGGCATCGGCAAGGTGCCGGGCATGATCGCCGTGGTGATCTATGCCGTGCCGCCCATGATCCG
CCTGACCAATCTCGGCATCCGGCTCGTCGATGCCGAGGTGCTGGAGGCGGCCGAGGCCTTCGGCTCGTCAGCGCGCCAGA
AGCTCTGGAACGTGCAGCTGCCGCTGGCGCTGCCCGCGCTGATGACCGGGGTGAACCAGACGATCATGATGTCGCTTTCG
ATGGTCGTCGTGGCCTCGATGGTGGGCGTGGGCGGTCTCGGCAAGAACGTGCTGCAGGCCATCAACAACCAGTTCTTCAC
GCTGGGCTTCATGAACGGCTTCGCCCTCGTGGCCATCGCCATCGTCTTCGACCGCACGAGCCAGGCCTTCGGCCGGCGGC
TGCAGAAACACCGGGAGGCCGCGCATGACTGA

Upstream 100 bases:

>100_bases
GACGTGGCCGACCGGGTCGCCGCGGCGCTCTGACCGCCCGACCCGAAGCCGGGCAGTCCCTGCCCGGCTTCGTTCATTGA
CCTTACCCGAGAGGAACAGG

Downstream 100 bases:

>100_bases
CCAGACCCTCGTGCAGATCGAGGACGAGGACGAGGCCCCCACCCTCCCGGGCCTCGAGATCCGCAACCTCTACAAGATCT
TCGGGCCCGACGGCGCCTCT

Product: ABC proline/glycine betaine transporter, inner membrane subunit

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MASACWSLPEAFCQFPALDGRTLRMMRKTIDGSFRDLVRSFSDAIDTATAPLQLALNAIERAFTSTPWIVVLAVLLAIVW
AGSRSWRIVAGSAVAMCAIGWFGLWEDTMVTLAMVSVCTVAAVIIGLPIGILAARSDRMQRLVNPVLDVMQTLPSFVYLI
PVVVIFGIGKVPGMIAVVIYAVPPMIRLTNLGIRLVDAEVLEAAEAFGSSARQKLWNVQLPLALPALMTGVNQTIMMSLS
MVVVASMVGVGGLGKNVLQAINNQFFTLGFMNGFALVAIAIVFDRTSQAFGRRLQKHREAAHD

Sequences:

>Translated_303_residues
MASACWSLPEAFCQFPALDGRTLRMMRKTIDGSFRDLVRSFSDAIDTATAPLQLALNAIERAFTSTPWIVVLAVLLAIVW
AGSRSWRIVAGSAVAMCAIGWFGLWEDTMVTLAMVSVCTVAAVIIGLPIGILAARSDRMQRLVNPVLDVMQTLPSFVYLI
PVVVIFGIGKVPGMIAVVIYAVPPMIRLTNLGIRLVDAEVLEAAEAFGSSARQKLWNVQLPLALPALMTGVNQTIMMSLS
MVVVASMVGVGGLGKNVLQAINNQFFTLGFMNGFALVAIAIVFDRTSQAFGRRLQKHREAAHD
>Mature_302_residues
ASACWSLPEAFCQFPALDGRTLRMMRKTIDGSFRDLVRSFSDAIDTATAPLQLALNAIERAFTSTPWIVVLAVLLAIVWA
GSRSWRIVAGSAVAMCAIGWFGLWEDTMVTLAMVSVCTVAAVIIGLPIGILAARSDRMQRLVNPVLDVMQTLPSFVYLIP
VVVIFGIGKVPGMIAVVIYAVPPMIRLTNLGIRLVDAEVLEAAEAFGSSARQKLWNVQLPLALPALMTGVNQTIMMSLSM
VVVASMVGVGGLGKNVLQAINNQFFTLGFMNGFALVAIAIVFDRTSQAFGRRLQKHREAAHD

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=256, Percent_Identity=45.703125, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1788451, Length=179, Percent_Identity=32.4022346368715, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788449, Length=189, Percent_Identity=32.2751322751323, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786564, Length=152, Percent_Identity=28.9473684210526, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32710; Mature: 32579

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASACWSLPEAFCQFPALDGRTLRMMRKTIDGSFRDLVRSFSDAIDTATAPLQLALNAIE
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAFTSTPWIVVLAVLLAIVWAGSRSWRIVAGSAVAMCAIGWFGLWEDTMVTLAMVSVCTV
HHHCCCCHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVIIGLPIGILAARSDRMQRLVNPVLDVMQTLPSFVYLIPVVVIFGIGKVPGMIAVVIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AVPPMIRLTNLGIRLVDAEVLEAAEAFGSSARQKLWNVQLPLALPALMTGVNQTIMMSLS
HHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHH
MVVVASMVGVGGLGKNVLQAINNQFFTLGFMNGFALVAIAIVFDRTSQAFGRRLQKHREA
HHHHHHHHCCCCCCHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHD
CCC
>Mature Secondary Structure 
ASACWSLPEAFCQFPALDGRTLRMMRKTIDGSFRDLVRSFSDAIDTATAPLQLALNAIE
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH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CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]