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The map label for this gene is gsiB [H]

Identifier: 77465047

GI number: 77465047

Start: 66628

End: 68187

Strand: Direct

Name: gsiB [H]

Synonym: RSP_3034

Alternate gene names: 77465047

Gene position: 66628-68187 (Clockwise)

Preceding gene: 77465046

Following gene: 77465048

Centisome position: 7.07

GC content: 67.24

Gene sequence:

>1560_bases
ATGACCGGACTCCCCCTGAGCCGACGCAGGTTCGTCCAGACCGCCGCCGCCGCGGGTCTTCTGATCCCCGCCGCGGGCCT
TGTCCCCGGTGCCGCGTTCGCGCAGGAGGGCCGGATCCTGCGGATCCGTAACGACGGCGACATGCGCCGCCTCGACCCGG
CGACCCGCGGCGGCTGGTATGACGAGACGGTGATGTTCTCGATCTTCTCCGGTCTCGTGCGCTACAAGGCCGGCGACGCG
TGGGGATGGGAGCTGGACGCCGCAGAGAGCATCGACGACAGCGATCCGCAGCATGTCAAATTCACCCTGCGCCCCGGCAT
CCAGTTCACCGGCGGTTTCGGCGAGATGACCGCCGAAGATGTGAAATTCTCCTACGAGCGCTTCCGGGATCCCGACGTCA
ATGCCGTCTATGGCGCAGATTGGGCGGCTCTCGACCATGTCGAGGTCACCGGCACCCATAGCGGCATCATCCACCTCAGC
CGCCCCTTCGCGCCGCTGTTCACCTCGACCCTGCCGCATGCTTCGGGGCTGATCCTGTCCAGGGCCGCGATGGAGGCCGC
GGGCAGGTCGGTGATCGACACCGATCCTCTGGCCTGTTCCGGCCCCTATCAGATCGCGGAATGGCGCCCGCGCGAGCTGA
TCCATCTGGTGCGCAACCCCGGCTGGGTCGGGCCCGCGCCCCATTTCGACGAGATCCGCCTGCTGCCGCTGACCGACCTG
ATCTCGGCCGAGACCGCCTTCGACGCCGGCGACATCGACGTCACGCAGATCGCGGTCAACTCCATCGCCGTGCGCGATGC
GGAGGCCGACAATCTGGTGGTCAAGCCGGCGCTGGCCTACACGTGGCTCGGCATGAACATCGAGAACGAGGTGCTGCTGG
ATCTGCGCATCCGCCGCGCGATCCAGCATGCGATCAACACCCGGGAGGTCGTCGAAGGCACCTTCGGCGAGGCGGTCACT
CCGGCCCATGGCCTGATCCCGCCGCCGCTGCTCGGCGCACGCGAGGCCAATCTCTACGGCTACGATCCCGACGCCGCCCG
TGCCCTGCTGGCCGAGGCCGGGGTGCCCGCGCTGCAGCTGACCCTGTCCTTCAACGGCGATCCTGACATGATGATCGCCG
CTCAGGTCATTCAGGCGCATCTGGCCGCCGTCGGCATCACGCTGGAGATCCGCGCGATGGATGGTGCGACGCTGACCGCG
CAGCAGCAGGACAGCGAAGGCGGCACCTACAAGGACATCCAGCTGTTCTTCTCGACCTTCACCACGGCCCCCGATCCCAG
CTGGGTCACCGAATGGTTCACCTGCGCCCAGATCGGCGACTGGAACTTCCAGCGCACCTGCTCGGAAGAGTGGGACAGCA
GGAATGCCGCCGCGGCCGAGGAGAGCGACCCGACCAGCCGCGCCGCCACCTATGTCGATCTGCAGAACCAGCTGGAAGAG
ACCGGCGCCTATGTCTTCCTGATGCATGGCGTCAATGCCTGGATCTCGAAGCCCGGGATCCGAACCGCCTACAGCGCCGA
TGCGCAATGGGCGCTGCTGCGCGACTTCGCGGCCGTGTGA

Upstream 100 bases:

>100_bases
CCGGCCCGCGGCCGCCTCGTGACCTGCCATCGGGCCGAAGAGCTGATGGCAGCCCCGCAATAACCACAACAACACCTCCC
AACAGCAGAGAAACATCATC

Downstream 100 bases:

>100_bases
TCCGGCCCGGCCCCTGCGGGGGCCGGCACCACGATCCGGGCCTGCCATCGCACGCCCGGCCCCCCTGTCTCAGTTGCACG
AACCGGCCGCCCGCCTCTGC

Product: ABC peptide/opine transporter, periplasmic substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MTGLPLSRRRFVQTAAAAGLLIPAAGLVPGAAFAQEGRILRIRNDGDMRRLDPATRGGWYDETVMFSIFSGLVRYKAGDA
WGWELDAAESIDDSDPQHVKFTLRPGIQFTGGFGEMTAEDVKFSYERFRDPDVNAVYGADWAALDHVEVTGTHSGIIHLS
RPFAPLFTSTLPHASGLILSRAAMEAAGRSVIDTDPLACSGPYQIAEWRPRELIHLVRNPGWVGPAPHFDEIRLLPLTDL
ISAETAFDAGDIDVTQIAVNSIAVRDAEADNLVVKPALAYTWLGMNIENEVLLDLRIRRAIQHAINTREVVEGTFGEAVT
PAHGLIPPPLLGAREANLYGYDPDAARALLAEAGVPALQLTLSFNGDPDMMIAAQVIQAHLAAVGITLEIRAMDGATLTA
QQQDSEGGTYKDIQLFFSTFTTAPDPSWVTEWFTCAQIGDWNFQRTCSEEWDSRNAAAAEESDPTSRAATYVDLQNQLEE
TGAYVFLMHGVNAWISKPGIRTAYSADAQWALLRDFAAV

Sequences:

>Translated_519_residues
MTGLPLSRRRFVQTAAAAGLLIPAAGLVPGAAFAQEGRILRIRNDGDMRRLDPATRGGWYDETVMFSIFSGLVRYKAGDA
WGWELDAAESIDDSDPQHVKFTLRPGIQFTGGFGEMTAEDVKFSYERFRDPDVNAVYGADWAALDHVEVTGTHSGIIHLS
RPFAPLFTSTLPHASGLILSRAAMEAAGRSVIDTDPLACSGPYQIAEWRPRELIHLVRNPGWVGPAPHFDEIRLLPLTDL
ISAETAFDAGDIDVTQIAVNSIAVRDAEADNLVVKPALAYTWLGMNIENEVLLDLRIRRAIQHAINTREVVEGTFGEAVT
PAHGLIPPPLLGAREANLYGYDPDAARALLAEAGVPALQLTLSFNGDPDMMIAAQVIQAHLAAVGITLEIRAMDGATLTA
QQQDSEGGTYKDIQLFFSTFTTAPDPSWVTEWFTCAQIGDWNFQRTCSEEWDSRNAAAAEESDPTSRAATYVDLQNQLEE
TGAYVFLMHGVNAWISKPGIRTAYSADAQWALLRDFAAV
>Mature_518_residues
TGLPLSRRRFVQTAAAAGLLIPAAGLVPGAAFAQEGRILRIRNDGDMRRLDPATRGGWYDETVMFSIFSGLVRYKAGDAW
GWELDAAESIDDSDPQHVKFTLRPGIQFTGGFGEMTAEDVKFSYERFRDPDVNAVYGADWAALDHVEVTGTHSGIIHLSR
PFAPLFTSTLPHASGLILSRAAMEAAGRSVIDTDPLACSGPYQIAEWRPRELIHLVRNPGWVGPAPHFDEIRLLPLTDLI
SAETAFDAGDIDVTQIAVNSIAVRDAEADNLVVKPALAYTWLGMNIENEVLLDLRIRRAIQHAINTREVVEGTFGEAVTP
AHGLIPPPLLGAREANLYGYDPDAARALLAEAGVPALQLTLSFNGDPDMMIAAQVIQAHLAAVGITLEIRAMDGATLTAQ
QQDSEGGTYKDIQLFFSTFTTAPDPSWVTEWFTCAQIGDWNFQRTCSEEWDSRNAAAAEESDPTSRAATYVDLQNQLEET
GAYVFLMHGVNAWISKPGIRTAYSADAQWALLRDFAAV

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=400, Percent_Identity=25.25, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787495, Length=533, Percent_Identity=23.0769230769231, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787762, Length=479, Percent_Identity=26.7223382045929, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1789966, Length=494, Percent_Identity=22.2672064777328, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789397, Length=500, Percent_Identity=22.8, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1789887, Length=479, Percent_Identity=22.3382045929019, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787551, Length=418, Percent_Identity=24.4019138755981, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56556; Mature: 56425

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLPLSRRRFVQTAAAAGLLIPAAGLVPGAAFAQEGRILRIRNDGDMRRLDPATRGGWY
CCCCCCHHHHHHHHHHHCCHHCCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCC
DETVMFSIFSGLVRYKAGDAWGWELDAAESIDDSDPQHVKFTLRPGIQFTGGFGEMTAED
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCHHH
VKFSYERFRDPDVNAVYGADWAALDHVEVTGTHSGIIHLSRPFAPLFTSTLPHASGLILS
HHHHHHHCCCCCCCEEECCCEEEECCEEEECCCCCEEEECCCCHHHHHHCCCCCCCHHHH
RAAMEAAGRSVIDTDPLACSGPYQIAEWRPRELIHLVRNPGWVGPAPHFDEIRLLPLTDL
HHHHHHCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHH
ISAETAFDAGDIDVTQIAVNSIAVRDAEADNLVVKPALAYTWLGMNIENEVLLDLRIRRA
HHHHCCCCCCCCCCEEEEEHHEEEECCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHH
IQHAINTREVVEGTFGEAVTPAHGLIPPPLLGAREANLYGYDPDAARALLAEAGVPALQL
HHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEE
TLSFNGDPDMMIAAQVIQAHLAAVGITLEIRAMDGATLTAQQQDSEGGTYKDIQLFFSTF
EEEECCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHH
TTAPDPSWVTEWFTCAQIGDWNFQRTCSEEWDSRNAAAAEESDPTSRAATYVDLQNQLEE
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHEEEHHHHHHHHH
TGAYVFLMHGVNAWISKPGIRTAYSADAQWALLRDFAAV
CCCEEEEEECCHHHHCCCCCCEEECCCCCHHHHHHHHCC
>Mature Secondary Structure 
TGLPLSRRRFVQTAAAAGLLIPAAGLVPGAAFAQEGRILRIRNDGDMRRLDPATRGGWY
CCCCCHHHHHHHHHHHCCHHCCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCC
DETVMFSIFSGLVRYKAGDAWGWELDAAESIDDSDPQHVKFTLRPGIQFTGGFGEMTAED
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCHHH
VKFSYERFRDPDVNAVYGADWAALDHVEVTGTHSGIIHLSRPFAPLFTSTLPHASGLILS
HHHHHHHCCCCCCCEEECCCEEEECCEEEECCCCCEEEECCCCHHHHHHCCCCCCCHHHH
RAAMEAAGRSVIDTDPLACSGPYQIAEWRPRELIHLVRNPGWVGPAPHFDEIRLLPLTDL
HHHHHHCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHH
ISAETAFDAGDIDVTQIAVNSIAVRDAEADNLVVKPALAYTWLGMNIENEVLLDLRIRRA
HHHHCCCCCCCCCCEEEEEHHEEEECCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHH
IQHAINTREVVEGTFGEAVTPAHGLIPPPLLGAREANLYGYDPDAARALLAEAGVPALQL
HHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEE
TLSFNGDPDMMIAAQVIQAHLAAVGITLEIRAMDGATLTAQQQDSEGGTYKDIQLFFSTF
EEEECCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHH
TTAPDPSWVTEWFTCAQIGDWNFQRTCSEEWDSRNAAAAEESDPTSRAATYVDLQNQLEE
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHEEEHHHHHHHHH
TGAYVFLMHGVNAWISKPGIRTAYSADAQWALLRDFAAV
CCCEEEEEECCHHHHCCCCCCEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA