Click here to switch to the map view.

The map label for this gene is garL [H]

Identifier: 77465027

GI number: 77465027

Start: 46296

End: 47048

Strand: Reverse

Name: garL [H]

Synonym: RSP_3014

Alternate gene names: 77465027

Gene position: 47048-46296 (Counterclockwise)

Preceding gene: 77465028

Following gene: 77465026

Centisome position: 4.99

GC content: 68.79

Gene sequence:

>753_bases
ATGCCCGGCGATCGCACCGGTTTCCGTGCCCGCCTGCGGGCGCCCGAGCCGATCCTCGGCACCTTCCTGAAGATCCCCTC
GACCCAGCCGGCCGAGATCCTCGCGACGCTCGGCTTCGACTTCGTCGTGATCGACGAGGAACATGCCCCCTTCAACCCGG
AAACCACCGATGCGATCCTGCTGGCCTGTCGCGCACACGGGATGGCCGGGCTGGTACGCGTGCGGCAACCGGCCGACATC
CTGCGGGTGCTCGACTGCGGGGCGGATGGGGTGCTCGTGCCGCATGTGGCATCGCCCGAGGCCGCCCGCGAGGCCGTCGC
CCTCGCGCGATACAGCGGAGCGCGCGGCCTGTCGCCCGCGACCCGTGCCGGCGCGTTCGGCGCGACGGATGTCGCGCAAC
ACATGAGACGGCAGGATCGACGCTGTGCCGTCATCGCGATGATGGAGGACAGGGAGTGCCTCGACAGGCTCGATGCGATC
CTCGCCACCGACGGACTGGACGCGATCTTCATCGGACGGGCGGATCTCGCCGCCAGCATGGGACCGGCAGCCGCAGACGG
AGGGATAGAGCGCGCGGTGGATCTTGTCCTCGCCAGCGCACGGGATGCCGGGATGCCTGCTCTTGTCCTCAGTTCCAACC
GCAGCGAGGCGGAGGAGTTTCGATCCAGAGGCGCGACCGGCTTCCTGAGCATGTCGGACCAGGGCTTCCTTCGAGAACAG
GCCGCCCGTATCCGCGCCATCTTTTCGGGATAG

Upstream 100 bases:

>100_bases
AGGTGCGCCGCATCCTCGAGGAGCTGGGCCATGAGATCGCCACTCCCGACGAGGCACGCAGGATGCTCGGCACCAAGGGC
GCAGGCGAGGTGGCTTTCTG

Downstream 100 bases:

>100_bases
GTTTGAACAGGCCCACGTCCGCATCCGACCAGCGCTCGCCCTGCCCGGGAAGGATGGCATAGGACACATCCGCTCCCATG
GGTCCGACCCGCTACGAGTG

Product: putative aldolase protein

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MPGDRTGFRARLRAPEPILGTFLKIPSTQPAEILATLGFDFVVIDEEHAPFNPETTDAILLACRAHGMAGLVRVRQPADI
LRVLDCGADGVLVPHVASPEAAREAVALARYSGARGLSPATRAGAFGATDVAQHMRRQDRRCAVIAMMEDRECLDRLDAI
LATDGLDAIFIGRADLAASMGPAAADGGIERAVDLVLASARDAGMPALVLSSNRSEAEEFRSRGATGFLSMSDQGFLREQ
AARIRAIFSG

Sequences:

>Translated_250_residues
MPGDRTGFRARLRAPEPILGTFLKIPSTQPAEILATLGFDFVVIDEEHAPFNPETTDAILLACRAHGMAGLVRVRQPADI
LRVLDCGADGVLVPHVASPEAAREAVALARYSGARGLSPATRAGAFGATDVAQHMRRQDRRCAVIAMMEDRECLDRLDAI
LATDGLDAIFIGRADLAASMGPAAADGGIERAVDLVLASARDAGMPALVLSSNRSEAEEFRSRGATGFLSMSDQGFLREQ
AARIRAIFSG
>Mature_249_residues
PGDRTGFRARLRAPEPILGTFLKIPSTQPAEILATLGFDFVVIDEEHAPFNPETTDAILLACRAHGMAGLVRVRQPADIL
RVLDCGADGVLVPHVASPEAAREAVALARYSGARGLSPATRAGAFGATDVAQHMRRQDRRCAVIAMMEDRECLDRLDAIL
ATDGLDAIFIGRADLAASMGPAAADGGIERAVDLVLASARDAGMPALVLSSNRSEAEEFRSRGATGFLSMSDQGFLREQA
ARIRAIFSG

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=247, Percent_Identity=32.7935222672065, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788578, Length=226, Percent_Identity=32.3008849557522, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 26511; Mature: 26379

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGDRTGFRARLRAPEPILGTFLKIPSTQPAEILATLGFDFVVIDEEHAPFNPETTDAIL
CCCCCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCHHHHH
LACRAHGMAGLVRVRQPADILRVLDCGADGVLVPHVASPEAAREAVALARYSGARGLSPA
HHHHHCCCHHHHHHCCHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCH
TRAGAFGATDVAQHMRRQDRRCAVIAMMEDRECLDRLDAILATDGLDAIFIGRADLAASM
HHCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEECHHHHHHC
GPAAADGGIERAVDLVLASARDAGMPALVLSSNRSEAEEFRSRGATGFLSMSDQGFLREQ
CCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCCEEEECCCCHHHHHH
AARIRAIFSG
HHHHHHHCCC
>Mature Secondary Structure 
PGDRTGFRARLRAPEPILGTFLKIPSTQPAEILATLGFDFVVIDEEHAPFNPETTDAIL
CCCCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCHHHHH
LACRAHGMAGLVRVRQPADILRVLDCGADGVLVPHVASPEAAREAVALARYSGARGLSPA
HHHHHCCCHHHHHHCCHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCH
TRAGAFGATDVAQHMRRQDRRCAVIAMMEDRECLDRLDAILATDGLDAIFIGRADLAASM
HHCCCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEECHHHHHHC
GPAAADGGIERAVDLVLASARDAGMPALVLSSNRSEAEEFRSRGATGFLSMSDQGFLREQ
CCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCCEEEECCCCHHHHHH
AARIRAIFSG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA