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The map label for this gene is trmJ [H]

Identifier: 77465011

GI number: 77465011

Start: 24699

End: 25430

Strand: Direct

Name: trmJ [H]

Synonym: RSP_3829

Alternate gene names: 77465011

Gene position: 24699-25430 (Clockwise)

Preceding gene: 77465009

Following gene: 77465012

Centisome position: 2.62

GC content: 69.13

Gene sequence:

>732_bases
ATGAGCATCGAACCCGTCTTCATCCTCGTCCGCCCGCAGATGGGCGAGAACATCGGTGCGGCCGCGCGCGCCATGCTGAA
CTTCGGCCTCGGCCGCCTGCGCATCGTCGATCCGCGCGACGGCTGGCCCAACCCGAAGGCGGTGGCCATGGCCTCGGGGG
CGGGGCGGCTTCTCGATCACGCCGGCCTCTTTCCCACGGTGGCCGAGGCGATCCGCGACTGCGACTATGTCTTCGCCACC
ACCGCCCGCGGGCGCGAGCTCACCAAGCCCGTGATGACGCCCGAGCGCGCCATGGCGCACGGCCGCGCGCTGACGGGCGA
GGGGCGGCGCGTGGGCATCCTCTTCGGGCCCGAGCGCACCGGGCTCGAGAACGAGGATGTGGCGCTGGCCAATGCCATCG
TGACCGTGCCGGTGAACCCCGAGTTCTTCTCGCTCAACCTCGCGCAATGCGTGCTGCTTCTGGCCTACGAATGGCGGCGC
CAGCATGACGAGACCCCGCCCGAGGTCATCGACATGGCGCGGGTGGATTTCGCGAGCGGCCTCGAGGTCGAGAAGCTCGG
CGATCATTTCGAGGAGAAGCTCGAGGCCGCGGGCTTCTTCTTCCCGCCCGAGAAGGCGCCGGGCATGAAGCTCAACCTGC
GCAACATGTGGGCCCGGCTGCCGCTGACGCGTGCCGACGTCCAGACCCTGCACGGGATGCTGCGCCAGATCGCCTGGAAG
CTGAAACAGTAG

Upstream 100 bases:

>100_bases
CGGATCGCGAAGCCTATAGCCGAGGGGCGCGCGCTTGGAAATGGCATTGCGCCGCAGGGCCCTTCTCCCCTAGGAAGCGC
CCGGATCCGAAGGGAGCGCC

Downstream 100 bases:

>100_bases
CGCCCGGCCGCTGCCGACCTTTGGATGAGGCTTTGCCCCGGCGAAACGTGCCAGAGTGGGCCCGCCAACAGGGAGGAAGA
CAATGGCGAGACTGCTCGTG

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSIEPVFILVRPQMGENIGAAARAMLNFGLGRLRIVDPRDGWPNPKAVAMASGAGRLLDHAGLFPTVAEAIRDCDYVFAT
TARGRELTKPVMTPERAMAHGRALTGEGRRVGILFGPERTGLENEDVALANAIVTVPVNPEFFSLNLAQCVLLLAYEWRR
QHDETPPEVIDMARVDFASGLEVEKLGDHFEEKLEAAGFFFPPEKAPGMKLNLRNMWARLPLTRADVQTLHGMLRQIAWK
LKQ

Sequences:

>Translated_243_residues
MSIEPVFILVRPQMGENIGAAARAMLNFGLGRLRIVDPRDGWPNPKAVAMASGAGRLLDHAGLFPTVAEAIRDCDYVFAT
TARGRELTKPVMTPERAMAHGRALTGEGRRVGILFGPERTGLENEDVALANAIVTVPVNPEFFSLNLAQCVLLLAYEWRR
QHDETPPEVIDMARVDFASGLEVEKLGDHFEEKLEAAGFFFPPEKAPGMKLNLRNMWARLPLTRADVQTLHGMLRQIAWK
LKQ
>Mature_242_residues
SIEPVFILVRPQMGENIGAAARAMLNFGLGRLRIVDPRDGWPNPKAVAMASGAGRLLDHAGLFPTVAEAIRDCDYVFATT
ARGRELTKPVMTPERAMAHGRALTGEGRRVGILFGPERTGLENEDVALANAIVTVPVNPEFFSLNLAQCVLLLAYEWRRQ
HDETPPEVIDMARVDFASGLEVEKLGDHFEEKLEAAGFFFPPEKAPGMKLNLRNMWARLPLTRADVQTLHGMLRQIAWKL
KQ

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=249, Percent_Identity=32.1285140562249, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1790865, Length=151, Percent_Identity=39.0728476821192, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26953; Mature: 26821

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEPVFILVRPQMGENIGAAARAMLNFGLGRLRIVDPRDGWPNPKAVAMASGAGRLLDH
CCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHH
AGLFPTVAEAIRDCDYVFATTARGRELTKPVMTPERAMAHGRALTGEGRRVGILFGPERT
CCCHHHHHHHHHHCCEEEEECCCCCHHHCCCCCHHHHHHCCCEECCCCCEEEEEECCCCC
GLENEDVALANAIVTVPVNPEFFSLNLAQCVLLLAYEWRRQHDETPPEVIDMARVDFASG
CCCCCCEEEEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
LEVEKLGDHFEEKLEAAGFFFPPEKAPGMKLNLRNMWARLPLTRADVQTLHGMLRQIAWK
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LKQ
HCC
>Mature Secondary Structure 
SIEPVFILVRPQMGENIGAAARAMLNFGLGRLRIVDPRDGWPNPKAVAMASGAGRLLDH
CCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHH
AGLFPTVAEAIRDCDYVFATTARGRELTKPVMTPERAMAHGRALTGEGRRVGILFGPERT
CCCHHHHHHHHHHCCEEEEECCCCCHHHCCCCCHHHHHHCCCEECCCCCEEEEEECCCCC
GLENEDVALANAIVTVPVNPEFFSLNLAQCVLLLAYEWRRQHDETPPEVIDMARVDFASG
CCCCCCEEEEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
LEVEKLGDHFEEKLEAAGFFFPPEKAPGMKLNLRNMWARLPLTRADVQTLHGMLRQIAWK
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LKQ
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA