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The map label for this gene is rfbD [H]

Identifier: 77461901

GI number: 77461901

Start: 6366179

End: 6367090

Strand: Direct

Name: rfbD [H]

Synonym: Pfl01_5681

Alternate gene names: 77461901

Gene position: 6366179-6367090 (Clockwise)

Preceding gene: 77461893

Following gene: 77461902

Centisome position: 98.88

GC content: 61.07

Gene sequence:

>912_bases
TTGAAAAAAAGTCTGTTCATCACGGGTCTCAGTGGATTCGTGGGACAACACATCCAGTCACGCCTGCAGGGGGAAGACGC
GTCGTGGGAGCTGCTGTCTGCCGCCTCACCCTACGACCTGACAAACCCGGACAGCCTCATCGACCTTTGGCCGCAACTGC
CCGATGCGGTCATTCATCTCGCCGGCCAGACCTTTGTCCCTGAAGCGTTCCGCGATCCGGCACGTACCTTCGACATCAAC
CTGCTCGGCACCCTGAACCTGTTGCAGGCCCTCAAGGCGCGCGGCTTCAAGGGCACGTTCCTGTATGTCAGCTCGGGCGA
TGTCTACGGCCAGGTCGAAGAACCCGCGCTGCCGATCACCGAACAACAACCACCCTGCCCGCGCAATCCTTACGCCGTGA
GCAAACTGTCGGCGGAATTTCTCAGCCTGCAATGGGGGTTGAGCGAAGGCTGGCCGGTGCTGGTGGCGCGTCCGTTCAAT
CACATCGGCACCGGGCAGAAGGACAGCTTCGTCATCGCCAGCGCCGCACGGCAGATCAACCGCATCAAGCAGGGGCTGCA
AGCGCCGCAACTGGAAGTCGGCGATATCGATGTCACCCGCGATTTCCTCGACGTCGGCGACGTGGTCTCGGCCTATTTTG
CGCTGCTGGAAAAAGGTACGCCGGGACAGGTCTACAACATTTGCTCAGGTCATGAGCAAAGCATCCGCAGCCTCATCGAA
CAGATGGGCGACCTGGCCCAGGTCAAGCTGCAACTGGTTCAGGATCCGGCACGCATGCGCCGCGCGGATCAGCGTCGCGT
CTGCGGCAGCCACCAACGGCTGGCCCGGACCACAGGATGGACGCCGCAAATCACTACACAACAATCCCTGCGGGCGATCC
TGTCCGACTGGGAGAAGCGAGTACAACAATGA

Upstream 100 bases:

>100_bases
GACTTCACCTACGGTTCTGCCGAATTATTCCTACGATCCGACAGCACATTCCCTTTAAGCCTGTGGGAAAGGTGAACGTT
AAAGACCAAGGGGTCGCTGT

Downstream 100 bases:

>100_bases
CAAAAAGTGCATTGATCACCGGGATCACCGGCCAGGACGGCGCGTATCTGGCCAAGCTGTTGCTCGACAAGGGTTACAAG
GTTCACGGCCTCGTGGCACG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKKSLFITGLSGFVGQHIQSRLQGEDASWELLSAASPYDLTNPDSLIDLWPQLPDAVIHLAGQTFVPEAFRDPARTFDIN
LLGTLNLLQALKARGFKGTFLYVSSGDVYGQVEEPALPITEQQPPCPRNPYAVSKLSAEFLSLQWGLSEGWPVLVARPFN
HIGTGQKDSFVIASAARQINRIKQGLQAPQLEVGDIDVTRDFLDVGDVVSAYFALLEKGTPGQVYNICSGHEQSIRSLIE
QMGDLAQVKLQLVQDPARMRRADQRRVCGSHQRLARTTGWTPQITTQQSLRAILSDWEKRVQQ

Sequences:

>Translated_303_residues
MKKSLFITGLSGFVGQHIQSRLQGEDASWELLSAASPYDLTNPDSLIDLWPQLPDAVIHLAGQTFVPEAFRDPARTFDIN
LLGTLNLLQALKARGFKGTFLYVSSGDVYGQVEEPALPITEQQPPCPRNPYAVSKLSAEFLSLQWGLSEGWPVLVARPFN
HIGTGQKDSFVIASAARQINRIKQGLQAPQLEVGDIDVTRDFLDVGDVVSAYFALLEKGTPGQVYNICSGHEQSIRSLIE
QMGDLAQVKLQLVQDPARMRRADQRRVCGSHQRLARTTGWTPQITTQQSLRAILSDWEKRVQQ
>Mature_303_residues
MKKSLFITGLSGFVGQHIQSRLQGEDASWELLSAASPYDLTNPDSLIDLWPQLPDAVIHLAGQTFVPEAFRDPARTFDIN
LLGTLNLLQALKARGFKGTFLYVSSGDVYGQVEEPALPITEQQPPCPRNPYAVSKLSAEFLSLQWGLSEGWPVLVARPFN
HIGTGQKDSFVIASAARQINRIKQGLQAPQLEVGDIDVTRDFLDVGDVVSAYFALLEKGTPGQVYNICSGHEQSIRSLIE
QMGDLAQVKLQLVQDPARMRRADQRRVCGSHQRLARTTGWTPQITTQQSLRAILSDWEKRVQQ

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=254, Percent_Identity=29.1338582677165, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4504031, Length=206, Percent_Identity=28.1553398058252, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI42516563, Length=328, Percent_Identity=26.8292682926829, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1788366, Length=290, Percent_Identity=26.551724137931, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788353, Length=341, Percent_Identity=25.5131964809384, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI48994969, Length=274, Percent_Identity=27.3722627737226, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786974, Length=275, Percent_Identity=26.5454545454545, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=278, Percent_Identity=24.8201438848921, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17539424, Length=278, Percent_Identity=24.8201438848921, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17539422, Length=278, Percent_Identity=24.8201438848921, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI133901788, Length=278, Percent_Identity=24.8201438848921, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI133901790, Length=278, Percent_Identity=24.8201438848921, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17507723, Length=280, Percent_Identity=25, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=315, Percent_Identity=27.3015873015873, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI115532424, Length=265, Percent_Identity=22.6415094339623, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=184, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24158427, Length=205, Percent_Identity=27.8048780487805, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356223, Length=319, Percent_Identity=26.3322884012539, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 33608; Mature: 33608

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLFITGLSGFVGQHIQSRLQGEDASWELLSAASPYDLTNPDSLIDLWPQLPDAVIHL
CCCCEEEECHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHH
AGQTFVPEAFRDPARTFDINLLGTLNLLQALKARGFKGTFLYVSSGDVYGQVEEPALPIT
CCCCCCCHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCCCCCC
EQQPPCPRNPYAVSKLSAEFLSLQWGLSEGWPVLVARPFNHIGTGQKDSFVIASAARQIN
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCHHCCCCCCCCCHHHHHHHHHH
RIKQGLQAPQLEVGDIDVTRDFLDVGDVVSAYFALLEKGTPGQVYNICSGHEQSIRSLIE
HHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHCCCCHHHHHHHHH
QMGDLAQVKLQLVQDPARMRRADQRRVCGSHQRLARTTGWTPQITTQQSLRAILSDWEKR
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
VQQ
HCC
>Mature Secondary Structure
MKKSLFITGLSGFVGQHIQSRLQGEDASWELLSAASPYDLTNPDSLIDLWPQLPDAVIHL
CCCCEEEECHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHH
AGQTFVPEAFRDPARTFDINLLGTLNLLQALKARGFKGTFLYVSSGDVYGQVEEPALPIT
CCCCCCCHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCCCCCC
EQQPPCPRNPYAVSKLSAEFLSLQWGLSEGWPVLVARPFNHIGTGQKDSFVIASAARQIN
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCHHCCCCCCCCCHHHHHHHHHH
RIKQGLQAPQLEVGDIDVTRDFLDVGDVVSAYFALLEKGTPGQVYNICSGHEQSIRSLIE
HHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHCCCCHHHHHHHHH
QMGDLAQVKLQLVQDPARMRRADQRRVCGSHQRLARTTGWTPQITTQQSLRAILSDWEKR
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
VQQ
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1372980; 7540582; 10952301 [H]